2nd edition. — Humana Press, 2022. — 316 p. — (Methods in Molecular Biology 2657). — ISBN 978-1-0716-3150-8. This second edition provides new and updated tools for studying protein-carbohydrate interactions ranging from traditional biochemical methods to state-of-the-art techniques. This book focuses on four different research themes detailing methods for screening and...
New York: Humana Press, 2023. — 331 p. This volume details protocols for computer-assisted design and experimental characterization of RNA nanostructures. Chapters guide readers through RNA nanotechnology, design and characterization of RNA nanostructures, assessment of immunology of nanomaterials, biosensing and drug delivery, various biomedical applications, and delivery...
New York: Humana Press, 2022. — 505 p. This meticulous book explores the leading methodologies, techniques, and tools for microarray data analysis, given the difficulty of harnessing the enormous amount of data. The book includes examples and code in R, requiring only an introductory computer science understanding, and the structure and the presentation of the chapters make it...
Humana Press, 1993. — 372 p. — (Methods in Molecular Biology 26). — ISBN: 0896032523. Protecting Groups in Oligonucleotide Synthesis Incorporation of Modified Bases into Oligonucleotides Functionalization of Oligonucleotides with Amino Groups and Attachment of Amino Specific Reporter Groups Functionalization of Oligonucleotides by the Incorporation of Thio-Specific Reporter...
Humana Press, 1993. — 496 p. —— (Methods in Molecular Biology 20). — ISBN: 0896032477. Leading researchers summarize and describe their experience and techniques for synthesizing oligonucleotides and their analogs. The protocols presented cover all DNA analogs that have been studied to date and constitute the single most extensive source of knowledge about DNA chemistry available.
New York: Humana, 2022. — 468 p. This detailed book compiles a series of laboratory protocols covering the most important aspects of R-loop biology. Beginning with a range of methods allowing for the detection of DNA-RNA hybrids, as well as their purification and visualization by electron microscopy, the volume continues with methods based on the use of RNase H-derived tools to...
2nd edition. — Humana Press, 2024. — 244 p. — (Methods in Molecular Biology 2722). — ISBN 978-1-0716-3477-6 This second edition provides detailed techniques used for the study and characterization of the plant vascular system, with a central focus on the xylem tissue. Chapters are organized in three main sections covering; analysis of xylem development, xylem characterization...
2nd edition. — Humana Press, 2024. — 244 p. — (Methods in Molecular Biology 2722). — ISBN 978-1-0716-3476-9. This second edition provides detailed techniques used for the study and characterization of the plant vascular system, with a central focus on the xylem tissue. Chapters are organized in three main sections covering; analysis of xylem development, xylem characterization...
ITexLi, 2024. — 155 p. — ISBN 1837682534 9781837682539 1837682097 9781837682096 1837682542 9781837682546. This volume is a comprehensive book that delves into the intricacies of Reactive Oxygen Species (ROS). It provides invaluable insights to researchers in the field, equipping them with the essential tools and knowledge to advance their work in this critical area, leading to...
Humana Press, 2014. — 313 p. — (Methods in Molecular Biology 1118). — ISBN: 978-1-62703-782-2; 978-1-62703-781-5. Cell-free protein expression promises to narrow the technological gap between DNA and protein technologies and provide a platform for broad application of synthetic biology principles in the Life Sciences. It is a rapid and high throughput methodology for the...
Humana Press, 2006. — 744 p. — (Methods in Molecular Biology 332). The complete sequencing of the human genome and many new microarray and proteomic methods for analyzing global changes in both gene expression and protein profiles have greatly increased our ability to understand transmembrane signaling processes in close detail. In this fully updated and expanded second edition...
New York: Humana Press, 2015. — 233 p. — (Methods in Molecular Biology 1234). The book is a compilation of a number of conceptual and methodological aspects important for the validation and characterization of intacrine signaling systems. To date, the best-characterized intracrine signaling system is that of angiotensin II (Ang II), covered in depth in various chapters....
Humana Press, 2013. — 230 p. — (Methods in Molecular Biology 1031). The innate immune system represents a critical arm of the immune response by providing immediate and robust host defense; however, human studies of its function are often limited by ethical, logistical, and technical obstacles. In this volume experts in the field explore the design and execution of experiments...
2nd Edition. — Springer, 2015. — 549 p. — (Methods in Molecular Biology 1284). — ISBN: 978-1-4939-2443-1. This second edition volume discusses the revolutionary development of faster and less expensive DNA sequencing technologies from the past 10 years and focuses on general technologies that can be utilized by a wide array of plant biologists to address specific questions in...
Humana Press, 2013. — 487 p. — (Methods in Molecular Biology 985). With the ultimate goal of systematically and robustly defining the specific perturbations necessary to alter a cellular phenotype, systems metabolic engineering has the potential to lead to a complete cell model capable of simulating cell and metabolic function as well as predicting phenotypic response to...
New York: Springer, 2021. — 207 p. In Neuronal Cell Culture: Methods and Protocols, the latest aspects of the culture of neural cells are explored by experts in the field who also explain the practical and theoretical considerations of the techniques involved. Starting with a general overview of the neuronal culturing principles that are described, this detailed volume covers...
Humana, 2024. — 230 p. This detailed book explores the interactions between different types of cells and immune cells, focusing on how their functionality is affected by immunosenescence. Beginning with traditional approaches, such as quantifying TRECs and measuring telomere lengths, the book continues with chapters on characterization of immune cell populations in aging,...
Humana Press, 2014. — 360 p. — (Methods in Molecular Biology 1169). The last decade has completely changed our understanding of pathogen recognition in terms of how the innate immune system manages to integrate molecular patterns of completely different pathogens into rather uniform innate immune responses. This immunostimulatory effect of pathogen-derived nucleic acids is of...
Humana, 2009. — 460 p. Grown exponentially by the genomic revolution, the use of the rat as a model of choice for physiological studies continues in popularity and at a much greater depth of understanding. In Rat Genomics: Methods and Protocols, world-wide experts provide both practical information for researchers involved in genomic research in the rat along with a more...
Humana Press, 2012. — 1021 p. — (Methods in Molecular Biology 855). Discovery of genetic material propelled the power of classical evolutionary studies across the diversity of living organisms. Together with early theoretical work in population genetics, the debate on sources of genetic makeup initiated by proponents of the neutral theory made a solid contribution to the...
Humana Press, 2021. — 390 p. — (Methods in Molecular Biology 2285). — ISBN 978-1-0716-1310-8. The aim of this volume is to provide a comprehensive description of methods and protocols useful for the further study of T-helper cells. Chapters guide readers through T-helper cell recovery, molecular study, signal transduction pathways, T-cell manipulation and, last but not least,...
Humana Press, 2018. — 351 p. — (Methods in molecular biology). — ISBN: 1493978187, 9781493978182, 1493978195, 9781493978199. This detailed book aims to address the need for standardizing key steps in plant metabolomics research that is still considered a critical issue in the field. The collection unites diverse mass spectrometry (MS)-based protocols, from sample preparation...
Humana Press, 2011. — 288 p. — (Methods in Molecular Biology 718). The recent expansion in diversity of RNA and DNA editing types has stimulated the development of many unique genetic, molecular, biochemical, and computational approaches to biological issues. In RNA and DNA Editing: Methods and Protocols, leading experts in the field introduce methods developed over the last...
ITexLi, 2021. — 231 p. — ISBN 1839627425 9781839627422 1839627441 9781839627446. This book contains information on epidemiology, resistance mechanisms, and alternative ways to curtail S. aureus infection, as well as future research opportunities. Staphylococcus aureus is a coccus, gram-positive, non-spore forming, and non-motile bacterium. Its commensal and opportunistic...
Humana Press, 2023 — 204 p. — (Methods in Molecular Biology 2610). — ISBN 978-1-0716-2894-2. This detailed volume spotlights methods to investigate a variety of virus-host interactions in humans, other mammals, fish, or insects. It explores viruses such as white spot syndrome virus (WSSV), honeybee viruses, Nipah virus, EBV, SVCV, HSV-1, HIV-1, A H1N1, and SARS-CoV-2, as well...
Humana Press, 2023. — 318 p. — (Methods in Molecular Biology 2632). — ISBN 978-1-0716-2995-6. This volume provides comprehensive dry and wet experiments, methods, and applications on nanopore sequencing. Chapters guide readers through bioinformatic procedures, genome sequencing, analysis of repetitive regions, structural variations, rapid and on-site microbial identification,...
Springer, 2016. — 208 p. — (Biological and Medical Physics, Biomedical Engineering). — ISBN 978-3-319-24446-4. Физические модели подвижности клеток Multicellular organisms are composed of individual cells that form the tissues, organs, and nervous system. In these organisms, the cells are replaced roughly every 100 days via controlled division and cell death. However, both...
Humana Press, 2014. — 197 p. — (Methods in Molecular Biology 1095). In this volume, expert researchers in the field detail many of the methods which are now commonly used to study miRNA maturation. These included established methods such as fluorescent and non-fluorescent methods for homogenous assays of Dicer-mediated miRNA maturation or an in vivo assay for Drosha activity....
3 rd edition. — Humana Press, 2012. — 642 p. — (Methods in Molecular Biology 824). — ISBN: 978-1-61779-432-2. This third edition of Recombinant Gene Expression is a lot more than just an update of the previous edition. Although some of the authors that contributed in 2004 were also invited to participate in this new project, this volume contains brand new protocols and topics...
Humana Press, 2018. - 394 p. - Volume 1737 of Methods in Molecular Biology. This volume details the most important methods used for studying prokaryotic non-coding RNAs and their protein accomplices. Chapters present methods in sections covering different aspects of the biology of that field: identification of ncRNAs, their differential expression, characterization of their...
New York: Humana, 2022. — 336 p. This detailed volume introduces different methods to assess the structure and folding of bacterial amyloids and analyze their functions, either in vitro or in vivo. Despite their initial association with neurodegenerative diseases, there is now increasing evidence of alternative roles for amyloids, with beneficial aspects for cells. In...
Humana Press, 2020. — 357 p. — (Methods in Molecular Biology 2113). — ISBN: 978-1-07-160277-5 This volume looks at the different spectroscopic and biophysical methods used by researchers to study the structure and folding of RNA, and to follow their interactions with proteins. The chapters in this book cover topics such as single-molecule spectroscopy of multiple RNA species;...
Humana Press, 2002. — 372 p. — (Methods in Molecular Biology 196). Cytochemical Localization of H 2 O 2 in Biological Tissues Localization of Intracellular Lipid Hydroperoxides Using the Tetramethylbenzidine Reaction for Transmission Electron Microscopy The Immunohistochemical Localization of Glutathione Peroxidase 4-Hydroxy-2-Nonenal (4-HNE) Staining by Anti-HNE Antibody...
Springer, 2021. — 340 p. — (Methods in Molecular Biology 2247). — ISBN-13 978-1-0716-1125-8. This volume explores strategies and detailed protocols for the preparation of macromolecular complexes and their characterization in view of structural analysis. The chapters in this book are separated into three parts: Part One focuses on sample preparation, and covers strategies for...
New York: Humana Press, 2015. — 355 p. This volume is designed to be a resource of proven techniques and approaches for probing the activities of bacterial, eukaryotic, and archaeal RNA polymerases. This book features a collection of in vitro and in vivo technologies that will permit researchers to purify and probe the position and stability of RNA polymerase complexes at...
Humana Press, 2023. — 472 p. — (Methods in Molecular Biology 2640). — ISBN 978-1-0716-3035-8. This volume looks at the latest technologies and methods--combined with new genetic tools available in animal models--used in this constantly evolving field. The chapters in this book are organized into three sections: Section one covers muscle stem cells and progenitor cells; Section...
Humana, 2009. — 272 p. In past decades, the area of bioinformatics has proved to be both dynamic and vital, producing a wide spectrum of novel approaches and assuming an increasingly important role in modern bio-technological development. In Biomedical Informatics, expert researchers explore cutting-edge new advances in the field, providing an overview of novel...
Humana Press, 2019. — 272 p. — (Methods in Molecular Biology 2063). — ISBN: 978-1-07-160137-2. This book volume provides in depth coverage of the nucleic acid field and aims to represent a broad diversity of the methodologies and a wide range of nucleic-acid-related topics within the fields of molecular biology and biomedicine. Chapters are divided in three sections detailing...
Humana Press, 2009. — 440 p. — (Methods in Molecular Biology 482). As regenerative medicine involves replacing diseased cells, tissues or organs, or repairing tissues in vivo, the manipulation of stem cells underlie its goals. In this volume leading experts in the field provide an updated representation of the landscape of stem cell-based therapies in a wide spectrum of tissue...
Humana Press, 2013. — 383 p. — (Neuromethods 74). — ISBN: 978-1-62703-103-5. The growth of the field of eating disorder research has led to a vast array of empirical articles, and the development of new animal models that can be used to study these disorders continues to stimulate new research. Animal Models of Eating Disorders serves as a collection of detailed techniques...
Springer, 2021. — 265 p. — (Methods in Molecular Biology 2255). — ISBN-13 978-1-0716-1161-6. This volume provides detailed protocols for the performance, analysis, and troubleshooting of in vitro and in vivo experiments related to programmed cell death. Chapters compile conventional techniques such as western blot and qPCR and state-of-the art transmission electron microscopy...
Humana Press, 2018. — 300 p. — (Methods in Molecular Biology 1693). This volume details the experimental approaches suitable for isolating and characterizing bacteriophages to formulating bacteriophage medicinal products and clinical application. Chapters guide readers through regulatory compliance and safety aspects of bacteriophage therapy. Written in the highly successful...
2nd edition. — Humana Press, 2024. — 316 p. — (Methods in Molecular Biology 2734). — ISBN 978-1-0716-3522-3. This highly anticipated second edition details cutting-edge experimental approaches for bacteriophage research. This comprehensive volume details new and updated experimental approaches for isolating, formulating, and engineering bacteriophage medicinal products....
2nd edition. — Humana Press, 2024. — 316 p. — (Methods in Molecular Biology 2734). — ISBN 978-1-0716-3523-0. This highly anticipated second edition details cutting-edge experimental approaches for bacteriophage research. This comprehensive volume details new and updated experimental approaches for isolating, formulating, and engineering bacteriophage medicinal products....
Humana Press, 2021. — 338 p. — (Methods in Molecular Biology 2246). — ISBN 978-1-0716-1114-2. This volume provides a comprehensive review of concepts and protocols related to fluorescence in situ hybridization (FISH) applied to microbial cells. Chapters will serve guide for the design of new probes and the development of novel FISH-based protocols. Written in the highly...
Humana Press, 2013. — 237 p. — (Methods in Molecular Biology 1023). Small proteins with molecular weights of <25 kDa are involved in major biological processes such as ribosome formation, stress adaption and cell cycle control. The study of the low-molecular-weight proteome has identified many central regulators of biology such as cytokines, chemokines, peptide hormones and...
Humana Press, 2014. — 268 p. — (Methods in Molecular Biology 1098). The book distills a wide range of techniques that use bioluminescence imaging as a tool for visualizing and tracking various biological processes. Covering diverse fields such as cellular and molecular biology, oncology, neurology, infectious diseases, immunology, and others, the detailed chapters of this volume...
Humana Press, 2013. — 344 p. — (Methods in Molecular Biology 952). All three peroxisome proliferator-activated receptor (PPAR) subtypes share a high degree of structural homology but differ in function, tissue distribution and ligand specificity. PPARs play critical roles as regulators of numerous physiological as well as pathophysiological pathways, and efforts are currently...
Humana Press, 2023. — 237 p. — (Methods in Molecular Biology 2606). — ISBN 978-1-0716-2878-2. This volume explores base editors (BEs), an invaluable CRISPR-based genome editing tool with a wide variety of versatile applications. Beginning with an overview of BEs, their diverse variants, and computational tools, the book continues with experimental applications of BEs for...
Humana Press, 2021. — 275 p. — (Methods in Molecular Biology 2195). — ISBN 978-1-0716-0859-3. This volume presents the latest techniques used to study germ cell tumors (GCTs), including histopathology; in vitro and in vivo techniques for investigating GCT biology; novel methods for detecting minimal residual diseases; and sequencing considerations as they apply to GCTs....
Humana Press, 2016. - 225 p. - Volume 1444 of Methods in Molecular Biology. This detailed volume includes a rich variety of applications using various instrumentations, probes, disease models, and targets in order to account for the multidisciplinary nature of the use of in vivo fluorescence imagine. The book also includes chapters on the emerging fields of cell tracking,...
Humana Press, 2006. — 704 p. Series: Methods in Molecular Biology, № 350 Protein Folding Protocols presents protocols for studying and characterizing protein folding from the unfolded to the folded state. Covering experiment and theory, bioinformatics approaches, and state-of-the-art simulation protocols for better sampling of the conformational space, this volume describes a...
New York: Humana, 2022. — 243 p. — ISBN 978-1-0716-2512-5, ISBN 978-1-0716-2513-2. This volume discusses the latest techniques used to identify cancer drug resistance determinants at the molecular, cellular, and functional levels. Chapters in this book cover up-to-date topics including tumor-microenvironment cell co-culture methods and microfluidics systems; workflows for...
2nd edition. — Humana Press, 2022 — 817 p. — (Methods in Molecular Biology 2303). — ISBN 978-1-0716-1397-9. This second edition volume expands on the previous edition with new information on the structural analysis of glycosaminoglycans (GAGs); chemical and enzymatic synthesis of GAGs; biophysical, biochemical, and computational analysis of GAG-protein interactions; molecular...
Humana Press, 2019. - 347 p. - Volume 1999 of Methods in Molecular Biology. The volume outlines techniques used to study the primary mechanisms of important DNA repair pathways. The chapters in this book are organized into five sections, each discussing a specific aspect of repair biology. Part I reviews the role of post-translational modifications of HDR, and DNA damage caused by...
Humana Press, 2000. — 233 p. A strong cohort of established investigators from a variety of disciplines detail their best techniques for advancing NPY research. Among the many readily reproducible methods described are those for cloning, for the production of transgenic and knockout models, and for the use of antisense technology to define the receptors and the signal...
2 nd ed. — Humana Press, 2004. — 523 P. — E-ISBN: 1-59259-774-2. General issues about recombinant gene expression . Host Cell Compatibility in Protein Expression. Production of Recombinant Proteins: Challenges and Solutions. Folding-Promoting Agents in Recombinant Protein Production. Prokaryotes . Back to Basics: pBR322 and Protein Expression Systems in E.сoli....
Humana Press, 2017. — 581 p. — ISBN10: 1493968793. — ISBN13: 978-1493968794. Series: Methods in Molecular Biology, № 1584 This volume provides all the essential protocols that are currently used to study the immune synapse. Chapters in The Immune Synapse: Methods and Protocols cover methods for the study of the dynamics of immune synapse assembly, traffic at the immune synapse,...
2nd edition. — Humana Press, 2023. — 518 p. — (Methods in Molecular Biology 2654). — ISBN 978-1-0716-3134-8. This new collection features the most up-to-date essential protocols that are currently being used to study the immune synapse. Beginning with methods for making biophysical measurements, the volume continues by covering the cell biology of synapses, methods for advanced...
2nd edition. — Humana Press, 2023. — 1213 p. — (Methods in Molecular Biology 2654). — ISBN 978-1-0716-3135-5. This new collection features the most up-to-date essential protocols that are currently being used to study the immune synapse. Beginning with methods for making biophysical measurements, the volume continues by covering the cell biology of synapses, methods for...
Humana Press, 2013. — 233 p. Series: Methods in Molecular Biology, № 995 Many advances in modern neuroscience are enabled by the availability of chemical tools that allow sensitive, precise, and quantitative measurements of, and control over, biological processes. These powerful reagents are widely used for investigating the nervous system at levels of detail ranging from ion...
Humana Press, 2024. — 256 p. Induced pluripotent stem cells (iPSCs) have become a useful in vitro disease model and a potential therapeutic cell source for ex vivo gene therapy due to their unlimited proliferation capacity and multidirectional potential. To avoid invasive sampling, several methods have been developed to generate human iPSCs from urine-derived cells. Classical...
Humana, 2023. — 191 p. This second edition volume expands on the previous edition with in-depth discussions on the rapid advancements in epithelial cell biology, and the cutting-edge research and techniques used by researchers in the field. The chapters in this book cover topics such as detailed methodologies applicable to epithelial cells derived from primates, pigs, bovines,...
Humana Press, 2023. — 286 p. — (Methods in Molecular Biology 2770). — ISBN 978-1-0716-3697-8. This detailed volume explores techniques for the isolation, purification, and establishment of in vitro germ cell (GS) systems at different stages of development in a variety of organisms, including chickens, mice, rats, and humans. The book additionally describes cutting-edge analytic...
Humana Press, 2023. — 286 p. — (Methods in Molecular Biology 2770). — ISBN 978-1-0716-3698-5. This detailed volume explores techniques for the isolation, purification, and establishment of in vitro germ cell (GS) systems at different stages of development in a variety of organisms, including chickens, mice, rats, and humans. The book additionally describes cutting-edge analytic...
Humana, 2008. — 273 p. RNA interference (RNAi), in which RNA silences RNA, is the most recent discovery to revolutionize the study of biology. In RNAi: Design and Applications, leaders in the field contribute state-of-the-art, easy to follow methods and bench protocols designed for practical, everyday use of RNAi in biological research. Divided into two parts, this...
Humana, 2010. — 242 p. Inositol, in its native or lipid derived forms, serves as a master building block which, when phosphorylated, leads to the construction of more than 30 unique isomeric forms that are employed in vital but diverse regulatory roles in cells. In Inositol Phosphates and Lipids: Methods and Protocols, expert researchers introduce the basic methodological tools...
Humana Press, 2005. — 481 p. — (Methods in Molecular Medicine 105). — ISBN: 978-1-58829-296-4. Over the past decade technical and conceptual breakthroughs, cross-species comparisons, and multidisciplinary approaches have led to a virtual revolution in developmental biology. In Developmental Hematopoiesis: Methods and Protocols, a panel of expert investigators critically surveys...
Humana Press, 2025. — 347 p. Preface Contributors Molecular Profiling of Chromatin and Genome Composition Whole-Genome Bisulfite Sequencing with a Small Amount of DNA Quantitative Profiling of Histone Variants and Posttranslational Modifications by Tandem Mass Spectrometry in Arabi... HiChIP for Plant Tissues A Practical Approach to High-Throughput and Accurate...
Humana, 2012. — 293 p. — ISBN-13 978-1617799174 Carotenoids are a family of yellow to orange-red terpenoid pigments synthesized by photosynthetic organisms and many bacteria and fungi. They have beneficialhealth effects protecting against oxidative damage, and may be responsible for the colours associated with plants and animals. In Microbial Carotenoids From Fungi: Methods and...
Humana Press, 2021. — 443 p. — (Methods in Molecular Biology, 2296). — ISBN 978-1-0716-1357-3. Antimicrobial resistance will become a global health threat since antimicrobial treatments continue at the forefront of the defense against microbial infections. To respond to the issue, this detailed book explores vital methodologies currently in use to advance our understanding of...
Humana Press, 2021. — 443 p. — (Methods in Molecular Biology, 2296). — ISBN 978-1-0716-1357-3. Antimicrobial resistance will become a global health threat since antimicrobial treatments continue at the forefront of the defense against microbial infections. To respond to the issue, this detailed book explores vital methodologies currently in use to advance our understanding of...
2nd edition. — Humana Press, 2023. — 351 p. — (Methods in Molecular Biology 2704). — ISBN13 9781071633847. — ISBN10 1071633848. This detailed volume explores experimental laboratory procedures for a wide range of steroid bioconversions. After an overview on the current trends and perspectives, the book continues with sections covering microbial screening and synthetic biology...
Humana Press, USA, 2016. — 211 p.— ISBN: 1493963910 Series: Methods in Molecular Biology, №1481 Now poised to evaluate the potential in modulating Wnt signaling for therapeutic agendas in cancer, wound healing, and degenerative disease, this book collects protocols from an exciting area of study. The methodologies include using Wnt modulating chemicals in engineering tissues...
New York: Humana Press, 2023. — 346 p. This volume provides classic and new methods to study the structure, assembly pathway, and protein synthesis ability of mitoribosomes across species. Following an introduction of fundamental concepts on the topic, method chapters present detailed protocols based on cryo-electron tomography, cryo-EM approaches, mitoribosome purification...
Humana Press, 2013. — 617 p. — (Methods in Molecular Biology 947). Teratology is the study of chemical-induced birth defects. This book is a comprehensive guide to the procedures and methods commonly employed in the safety testing of all classes of chemical for teratogenicity (also referred to as embryotoxicity, developmental toxicity or prenatal toxicity). The various...
Humana Press, 2021. — 472 p. — ISBN 978-1-0716-1361-0. This volume explores analytical methods to study complex lipid mixtures from plants and algae. The chapters in this book are organized into five parts and cover topics such as basic methods of lipid isolation and analysis; mass spectrometry and NMR analysis; lipid isolation and analysis from plant tissues, cell compartments...
Humana Press, 2021. — 472 p. — ISBN 978-1-0716-1361-0. This volume explores analytical methods to study complex lipid mixtures from plants and algae. The chapters in this book are organized into five parts and cover topics such as basic methods of lipid isolation and analysis; mass spectrometry and NMR analysis; lipid isolation and analysis from plant tissues, cell compartments...
New York: Humana Press, 2023. — 265 p. This second edition volume expands on the previous edition with discussions on the latest approaches used in the spectral cytometry field. Beginning with a brief history of spectral cytometry development, this book continues with a section addressing new protocols in different areas of imaging cytometry based on the unique technology of...
2nd edition. — Humana Press, 2003. — 519 p. — (Methods in Molecular Biology 226) — ISBN: 978-0-89603-627-7 Drawing on the proven qualities of the much praised and widely used first edition, John M. S. Bartlett and David Stirling have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research...
3rd edition. — Humana Press, 2022. — 270 p. — (Methods in Molecular Biology 2392). — ISBN 978-1-0716-1798-4. This third edition provides new and updated chapters on design PCR primers for successful DNA amplification. Chapters are divided into seven parts, including primer design strategies for quantitative PCR, genotyping, multiplex PCR, in silico PCR primer design, and primer...
Humana Press, 2013. — 393 p. — (Methods in Molecular Biology 1001). Tissue engineering and regenerative medicine represents a wide array of cell, biomaterial and cell/biomaterial based approaches focusing on the repair, augmentation, and regeneration of diseases tissues and organs. The book has been assembled in response to the growing interest in organ and tissue regeneration as...
Humana Press, 2015. — 329 p. Series: Methods in Molecular Biology, № 1245 The ability to produce vast amounts of DNA sequence data has enabled the discovery of molecular markers in model organisms, crops, as well as orphan species making genotyping the rate limiting factor, and this volume focuses on the different markers available and the low to high throughput genotyping of...
Humana Press, 2019. - 294 p. - Volume 2054 of Methods in Molecular Biology. This volume explores disease diagnosis and therapy in developing theranostics. The chapters in this book are divided into four parts and cover various techniques used in studying bioengineering, molecular diagnostics, in vivo imaging, and imaging-guided therapy. Written in the highly successful Methods in...
Humana Press, 2010. — 276 p. — (Methods in Molecular Biology 608). — ISBN: 978-1-59745-363-9 (eBook), 978-1-58829-950-5 (Hardcover). Recent work has revealed that stabilizing G-quadruplexes in telomeric DNA inhibits telomerase activity, providing impetus for the development of G-quartet-interacting drugs, while G-quartet-containing oligonucleotides have been recognized as a...
Humana Press, 2014. — 239 p. Series: Methods in Molecular Biology, № 1155 Exploring these type II trans-membrane proteins, this volume focuses on various techniques to investigate aspects of the TNF Superfamily members in health and disease. Opening with protocols to understand the signaling process of TNF family members, this detailed volume continues with technical examples of...
New York: Humana Press, 2015. — 288 p. Although nuclear reprogramming-the cellular process underlying cloning and the artificial generation of stem cells from somatic cells-may be considered a "black box" whose interior events are either completely unknown or at least imperfectly characterized, tantalizing clues about its operation are beginning to emerge. In Nuclear...
Humana Press, 2018. — 353 p. — (Methods in Molecular Biology 1765). — ISBN: 978-1-4939-7764-2; 978-1-4939-7765-9 This volume explores the latest developments in the study of the mechanisms, diagnostics, screening methods, and therapeutics of colorectal cancer. The book’s chapters are divided into three parts: the chapters in Part One examine techniques used to study the molecular...
Humana Press, 2013. — 373 p. Series: Methods in Molecular Biology, № 988 Glyco-engineering is being developed as a method to control the composition of carbohydrates and to enhance the pharmacological properties of monoclonal antibodies (mAbs) and other proteins. In this volume experts in the field provide readers with production and characterization protocols of glycoproteins...
Humana Press, 2013. — 235 p. — (Methods in Molecular Biology № 975). Plants are amazing organisms to study, some are important sources for pharmaceuticals, and others can help to elucidate molecular mechanisms required for a plant’s development and its interactions with the biotic or abiotic environment. Functional genomics is vastly lagging behind the speed of genome...
New York: Humana Press, 2014. — 365 p. Notch Signaling: Methods and Protocols focuses on molecular, biochemical and bioinformatics aspects of Notch signaling. Chapters analyze the Notch interactome, post-translational modifications of Notch, ligand binding assays, methods to assess proteolytic cleavage and transcriptional targets. Written in the highly successful Methods in...
Humana Press, 2019. — 223 p. — (Methods in Molecular Biology 2007). — ISBN 978-1-4939-9527-1. This book explores techniques for exploring hydrogen sulfide (H2S) and its effects on the vascular system through numerous experimental animal models and vascular preparations. Alterations of vascular H2S generation/signaling may be involved in the pathogenesis of systemic and...
Humana Press, 2019. - 294 p. - Volume 1938 of Methods in Molecular Biology. This detailed volume gathers together a broad variety of methods essential to the investigation of the biology of astrocytes and their multifaceted roles in both healthy and diseased brains. Beginning with some overviews of the subject, the book continues by covering techniques for the isolation of...
Humana Press, 2008. — 536 p.
Series: Methods in Molecular Biology, № 395
The book provides a collection of robust protocols for molecular biologists beginning to use comparative genomic analysis tools in a variety of areas. Volume I contains the first four of seven sections. In the first section, the reader is introduced to genomes via a number of visualization tools that...
Humana Press, 2007. — 426 p.
Series: Methods in Molecular Biology, № 396
The book provides a collection of robust protocols for molecular biologists beginning to use comparative genomic analysis tools in a variety of areas. Volume 2 contains the last three of seven sections. In the second volume, the fifth section describes a number of tools for comparative analysis of domain...
Humana Press, 2018. — 281 p. — (Methods in Molecular Biology 1687). — ISBN 978-1-4939-7373-6. This volume explores experimental approaches used to study Duchenne muscular dystrophy (DMD), an X-linked degenerative skeletal muscle disease caused by mutations in the dystrophin gene. Including the latest progress and scientific achievements, the book covers recent discoveries...
Humana Press, 2014 — 402p. — (Methods in Molecular Biology 1115) — ISBN: 978-1-62703-767-9 (eBook), 978-1-62703-766-2 (Hardcover). Plant taxonomy is an ancient discipline facing new challenges with the current availability of a vast array of molecular approaches which allow reliable genealogy-based classifications. Although the primary focus of plant taxonomy is on the...
2nd edition. — Humana, 2021. — 407 p. — (Methods in Molecular Biology, 2222). — ISBN 978-1-0716-0996-5. This fully updated edition explores conceptual as well as technical guidelines for plant taxonomists and geneticists, such as the increasing use of next-generation sequencing (NGS) technologies for numerous applications in plant taxonomy. The volume provides molecular...
Humana Press, 2023. — 267 p. — (Methods in Molecular Biology 2701). — ISBN 978-1-0716-3372-4. This detailed volume provides a comprehensive set of experimental protocols and useful strategies to examine the repair of damaged bases via the Base Excision Repair (BER) pathway in vitro and in cells. Beginning with multiple molecular and cellular techniques to examine the excision...
Humana Press, 2017. — 309 p. — (Methods in Molecular Biology 1679). — ISBN: 978-1-4939-7337-8. This detailed volume provides background on recent new technology developments highlighting the potential of the genomic era in wheat breeding with invaluable instruction on the methodology, which is complemented by overview chapters on the status of new technology application in...
Humana, 2020. — 539 p. This book provides detailed information on methodologies used in biological, serological and nucleic acid based assays for the detection, diagnosis and management of plant viruses. The content is divided into six main parts, the first of which presents techniques used in the biological characterization and transmission of viruses, while Part II covers...
2nd edition. — Humana Press, 2023. — 375 p. — (Methods in Molecular Biology 2625). — ISBN 978-1-0716-2965-9. This fully updated book presents an account of areas of utility, techniques, and bioinformatic advancements in the field of lipidomics. Beginning with protocols for lipid isolation and extraction, the volume continues with techniques from extractive mass spectrometry to...
Humana Press, 2014. — 245 p. Series: Methods in Molecular Biology, № 1160 The book focuses on different applications of qPCR ranging from microbiological detections (both viral and bacterial) to pathological applications. Several chapters deal with quality issues which regard the quality of starting material, the knowledge of the minimal information required to both perform an...
2nd ed. — Humana, 2025. — 374 p. — ISBN-13 : 978-1071645093 This second volume provides protocols describing the isolation and culture of diverse cell types stemming from the skin and the use of these cells and cell constructs for wound healing, bioengineering applications, and translational medicine purposes. Chapters describe the isolation, culture of diverse skin cells, skin...
Humana Press, 2015. — 263 p. — (Neuromethods 103). — ISBN: 978-1-4939-2509-4. This volume presents cutting-edge techniques to design, fabricate and use compartmentalized microfluidic devices. Chapters encompass axon guidance and manipulation, compartmentalized devices for synapse manipulation, cell populations interaction either in physiological or pathological condition and...
Humana Press, 2013. — 395 p. — ISBN: 978-1-62703-283-4. Series: Methods in Molecular Biology, № 977 The goal of the book is to provide scientists in academia, food, and pharmaceutical industry, as well as public institutions, complementary technologies for investigating various facets of regulatory systems that contribute to the control of protein- coding genes in mammalian cells.
New York: Humana Press, 2017. — 270 p. This volume provides a collection of protocols aimed toward the study of ethylene signaling in plants. Ethylene Signaling: Methods and Protocols is divided into three sections: ethylene biosynthesis, the signal transduction pathway, and the diverse ethylene responses of dicots and monocots. The chapters in section one discuss techniques...
Humana Press, 2014 — 2nd Ed. — 306p. — (Methods in Molecular Biology 1127) — ISBN: 978-1-62703-986-4 (eBook), 978-1-62703-985-7 (Hardcover).
Plant-Pathogen Interactions: Methods and Protocols, Second Edition expands upon the first edition with current, detailed protocols for the study of plant pathogen genome sequences. It contains new chapters on techniques to help identify...
Springer Science+Business Media LLC., 2017. — 265 p. — (Methods in Molecular Biology 1616) — ISBN: 1493970356. This volume provides a comprehensive collection of protocols on molecular diagnostics of bacteria that will suit the needs of molecular biologists, clinical laboratorians, and physician scientists alike. Chapters detail common bacterial pathogens, protocols that can be...
Humana Press, 2019. - 337 p. - Volume 1946 of Methods in Molecular Biology. This detailed volume serves clinicians and basic science researchers studying the increasingly antibiotic resistant Gram-negative bacterium Acinetobacter baumannii. Chapters detail microbiological techniques, biochemical techniques, clinical samples, and next generation omics techniques to characterize...
Humana Press, 2018. — 382 p. — ISBN: 978-1-4939-7456-6 Series: Methods in Molecular Biology, № 1702 There are many definitions striving to capture the hidden meaning of “Systems Biology.” Indeed, despite many efforts, the concept of Systems Biology remains quite uncertain. As already highlighted, currently two primary streams can be recognized within Systems Biology: (1)...
Springer, 2015. — 263 p. — (Methods in Molecular Biology 1277). — ISBN: 978-1-4939-2376-2. This volume on metabonomics provides detailed information on the procedures involved in nuclear magnetic resonance (NMR) spectroscopy, gas chromatography-mass spectrometry (GS-MS), liquid chromatography-mass spectrometry (LC-MS), and capillary electrophoresis-mass spectrometry (CE-MS)....
Humana Press, 2015. — 339 p. — (Methods in Molecular Biology 1309). This volume provides the plant scientific community with a collection of established and recently developed experimental protocols to study plant gravitropism. The first few chapters in this book discuss topics such as methods to properly orient plant material for gravitropism studies; protocols for data...
Springer, 2022. — 673 p. This Open Access volume provides a comprehensive overview of the latest tools available to scientists to study the many facets of whole-body regeneration (WBR). The chapters in this book are organized into six parts. Part One provides a historical overview on the study of the WBR phenomena focusing on the primary challenges of this research. Parts Two...
Humana Press, 2021. — 322 p. — (Methods in Molecular Biology 2157). This detailed book collects methods based on the evolution of the chromosome conformation capture (3C) technique and other complementary approaches to dissect chromatin conformation with an emphasis on dissection of nuclear compartmentalization and visualization in imaging. Written for the highly successful...
Humana, 2024. — 300 p. This edition provides a collection of state-of-art methods and tools for human leukocyte antigen (HLA) and major histocompatibility complex (MHC) research. The book explores updated as well as novel in silico tools, resources, and wet lab protocols for HLA typing, including determination of the HLA class I and class II type of an individual in clinical...
Springer, 2020. — 309 p. — (Methods in Molecular Biology 2120). — ISBN: 978-1-0716-0326-0. This volume focuses on a variety of in silico protocols of the latest bioinformatics tools and computational pipelines developed for neo-antigen identification and immune cell analysis from high-throughput sequencing data for cancer immunotherapy. The chapters in this book cover topics...
Humana Press, 2021. — 322 p. — (Methods in Molecular Biology 2272). — ISBN 978-1-0716-1293-4. This volume explores the latest methods used to study various aspects of TET proteins and their biology. Chapters in this book are divided into five parts. Part One describes technologies aimed at detecting and quantifying DNA methylation turnover using massively parallel sequencing,...
Humana Press, 2018. - 351 p. - Volume 1722 of Methods in Molecular Biology/ This volume provides readers with the latest techniques and tools to assess modifications and functions of the surfaceome. The chapters in this book are divided into 4 sections: discovery-based approaches to surfaceome content; targeted approaches for surfaceome content; cell-based function analyses...
Humana Press, 2019. — 299 p. — (Methods in Molecular Biology 1986). This book provides a comprehensive, interdisciplinary collection of the main, up-to-date methods, tools, and techniques for microarray data analysis, covering the necessary steps for the acquisition of the data, its preprocessing, and its posterior analysis. Featuring perspectives from biology, computer science,...
Humana Press, 2016 — 165p. — (Methods in Molecular Biology 1393) — ISBN: 978-1-4939-3338-9 (eBook), 978-1-4939-3336-5 (Hardcover). This volume explores the still undiscovered secrets of tumor immunology and cancer immunology by discussing the methods and techniques that world-renowned experts in the field use in their laboratories. This book provides a better understanding of...
Humana Press, 2019. — 580 p. — (Methods in Molecular Biology 2022) — ISBN: 978-1-4939-9607-0 This volume explores the recent advancements in biomolecular simulations of proteins, small molecules, and nucleic acids, with a primary focus on classical molecular dynamics (MD) simulations at atomistic, coarse-grained, and quantum/ab-initio levels. The chapters in this book are...
Humana Press, 2019. — 1432 p. — (Methods in Molecular Biology 2022) — ISBN: 978-1-4939-9608-7 This volume explores the recent advancements in biomolecular simulations of proteins, small molecules, and nucleic acids, with a primary focus on classical molecular dynamics (MD) simulations at atomistic, coarse-grained, and quantum/ab-initio levels. The chapters in this book are...
Humana Press, 2018. — 373 p. — (Methods in Molecular Biology 1715). This detailed volume describes a spectrum of methods and protocols that can be used for the bench-to-bedside development and evaluation of retinal gene therapy. Methods for the successful delivery of these gene therapy vector systems to the retina are examined, as well as assays to test the efficacy in vitro in...
Humana Press, 2017. — 278 p. — (Methods in Molecular Biology 1639). — ISBN 978-1-4939-7161-9. This volume provides protocols that expand on the latest alpha-1-antitrypsin (AAT) research. The chapters in this book are divided in to three sections: Part I is dedicated to patient-oriented research; part II discusses animal models; and Part III focuses on in vitro studies. Written...
Humana Press, 2021. — 296 p. — ISBN 978-1-0716-1165-4. This volume provides readers with a comprehensive look at the latest techniques used to identify and characterize PDZ-mediated interactions. Chapters cover topics such as promiscuity, multimodularity, regulation, and viral recognition by PDZ domains. Written in the highly successful Methods in Molecular Biology series...
New York: Humana Press, 2022. — 367 p. This volume covers the most up-to-date methods and techniques used to further the understanding of chromaffin cell biology and pharmacology. Chapters guide readers through the basic mechanisms that regulate the stimulus-secretion coupling, chromaffin, tumor-derived cell PC-12 , morphology, biochemistry, pharmacology, electrophysiology, and...
Humana Press, 2021. — 376 p. — ISBN 978-1-0716-1596-6. This volume contains cutting-edge techniques to study the function of enhancers and promoters in depth. Chapters are divided into six sections and describe enhancer-promoter transcripts, nucleosome occupancy, DNA accessibility, chromatin interactions, protein-DNA interactions, functional analyses, and DNA methylation...
Humana Press, 2018. - 347 p. - Volume 1685 of Methods in Molecular Biology. This volume details basic and advanced protocols for both stages of protein engineering: the library design phase and the identification of improved variants by screening and selection. Chapters focus on enzyme engineering using rational and semi-rational approaches. Written in the highly successful...
Humana Press, 2016. — 186 p. — (Methods in Molecular Biology 1373).
This detailed book contains chapters using unbiased approaches such as chemical and transposon mutagenesis, as well as a protocol for allelic exchange, to make targeted mutants in Staphylococcus, as generating mutants of this bacteria can be accomplished in many ways, depending on the intended changes. Using...
2nd edition. — Humana Press, 2023. — 382 p. — (Methods in Molecular Biology 2580). — ISBN 978-1-0716-2739-6. This second edition volume provides new and updated chapters detailing simple and accessible experiment protocols to explore thymus biology. Chapters are divided into three parts presenting short reviews, analysis strategies, protocols for cell preparation, flow...
Humana Press, 2016. — 311 p. This volume provides simple and accessible experiment protocols to explore thymus biology. The book is divided into three parts presenting short reviews on T cell development, analysis strategies, protocols for cell preparation, flow cytometry analyses, and multiple aspects of thymocyte biology. As a volume in the highly successful Methods in Molecular...
Humana Press, 2017. — 357 p. — (Methods in Molecular Biology 1519). This volume details experimental approaches used to investigate phagocytosis and phagosome maturation. Chapters present methods and protocols on quantifying uptake and phagosome maturation using biophysical and biochemical approaches, proteomics, microscopy, and flow cytometry. Written in the highly successful...
2nd edition. — Humana Press, 2017. — 385 p. — (Methods in Molecular Biology 2692). — ISBN 978-1-0716-3337-3. This volume explores updated and entirely new experimental approaches used to investigate phagocytosis and phagosome maturation. In order to aid in the study of engulfment, maturation, resolution, and pathogen manipulation of phagocytes, the book features methodology to...
New York: Humana Press, 2016. — 248 p. This fully updated volume reflects the spectacular advances in our knowledge of signal transduction pathways with a selection of ‘classic’ as well as newly developed approaches. These detailed approaches expand into the fields of molecular biology, biochemistry, physiology, cell biology, genetics, and genomics. Written in the highly...
New York: Humana Press, 2015. — 485 p. This volume provides readers with current methods to study RNA remodeling proteins. The methods, ranging from basic to complex, help the scientific community understand the role and fate of RNA species in cells, and their structures and interactions with other biomolecules. The book begins with two introductory chapters, followed by chapters...
Springer, 2019. — 238 p. — (Methods in Molecular Biology 1899). — ISBN10: 1493989367. This edition provides a detailed, up-to-date overview of methods used in the field of immune tolerance. Chapters guide readers through tolerogenic cell types, isolating tolerogenic cell populations for study and therapeutic utility, multiple methods to study the mechanisms underpinning...
Humana Press, 2014. — 279 p. Series: Methods in Molecular Biology, № 1133 The book is a collection of laboratory protocols covering current methods that are employed to measure and detect activities of these proteases in diverse biological systems, ranging from unicellular organisms to mammals. Broken into two parts, the first part focuses on methods to measure, detect, and...
Humana, 2024. — 356 p. This detailed volume explores the production and purification of glycoproteins for therapeutic use or basic research, as well as the necessary in-depth understanding of cellular and acellular systems available for these purposes, their advantages and disadvantages, and considerations for choosing the most appropriate system for the desired application....
Humana Press, 2011. - 523 p. - Volume 736 of Methods in Molecular Biology. - With its ability to explore the surface of the sample by means of a local scanning probe and its use of dedicated software allows to be visualize results, atomic force microscopy (AFM) has revolutionized the study of the smallest aspects of life. Atomic Force Microscopy in Biomedical Research: Methods...
Humana Press, 2023. — 387 p. — (Methods in Molecular Biology 2607). — ISBN 978-1-0716-2882-9. The volume presents a small selection of state-of-the-art approaches for studying transposable elements(TE). Chapters guide readers through HTS-based approaches, bioinformatic tools, methods to studyTE protein complexes, and the functional impact on the host. Written in the successful...
Humana Press, 2012. — 614 p. — (Methods in Molecular Biology 845). Microbiologists, medical mycologists, immunologists, and biochemists are increasingly working together to focus on the processes involved in the progression and treatment of fungal disease. This volume is designed for research scientists who are involved in this work and interested in undertaking new or...
Humana, 2025. — 395 p. — ISBN-13 : 978-1071643778 This detailed volume collects techniques for increasing our understanding of wheat rust pathogens, the host plant, and the interactions between the two. Beginning with routine and advanced tools used for pathogen surveillance, characterization, and field assessment of germplasm for resistance, the book continues with sections on...
Humana Press, 2019. - 314 p. - Volume 1954 of Methods in Molecular Biology. This book provides a selection of recently developed methods and protocols in bacterial glycomics to aid in bettering our understanding of the structures and functions of bacterial polysaccharides, their attachments to proteins and lipids, their role in biofilm formation, as well as their biosynthesis....
Humana Press, 2013. — 445 p. — (Methods in Molecular Biology 1022). Glycosyltransferases (GTs) are essential for the biosynthesis of complex glycoconjugates and are powerful tools to study the functions of complex glycans in health, development and disease. Complex glycoconjugates, such as glycoproteins, proteoglycans and glycolipids, are assembled by GTs which synthesize...
Humana Press, 2006. - 401 p. Series: Methods in Molecular Biology, №347 Glycobiology involves studies of complex carbohydrates and posttrans- tional modifications of proteins, and has become an important interdiscip- nary field encompassing chemistry, biochemistry, biology, physiology, and pathology. Although initial research was directed toward elucidation of the different...
Humana Press, 2019. — 308 p. — (Methods in Molecular Biology, №1983). — ISBN: 978-1-4939-9433-5. The book focuses on the regulation of protein function and biological activity by the post-translational modification known as acetylation. Chapters detail a wide range of topics in protein acetylation dealing with pathways relevant to cellular homeostasis, gene expression, and DNA...
Humana Press, 2016 — 228p. — (Methods in Molecular Biology 1396) — ISBN: 978-1-4939-3344-0 (eBook), 978-1-4939-3342-6 (Hardcover). This volume combines protocols that encompass the true variety of investigation done on superantigens in the fields of microbiology, immunology, molecular biology, biochemistry, and cellular biology, with a strong focus on disease models utilized to...
Humana, 2024. — 279 p. — ISBN-13 : 978-1071639016 This volume covers the latest advancements in the study of lipid signaling. The chapters in this book cover topics such as neuronal cells such as retinal ganglion cells, microglial cells to muscle, and epithelial cells; extraction for signaling studies, including clinical studies in humans; and discussions on different cells,...
Humana Press, 2019. — 294 p. — (Methods in Molecular Biology 2042). — ISBN: 978-1-4939-9693-3. This book explores cutting-edge methods to work with the notoriously difficult, but highly prevalent, obligate intracellular pathogen, Chlamydia trachomatis. These include techniques to identify Chlamydia trachomatis in patient samples, ranging from simple point-of-care tests to whole...
Humana Press, 2021. — 349 p. — (Methods in Molecular Biology 2130). — ISBN 978-1-0716-0380-2. This volume presents techniques used by researchers from all branches of biology to study daily changes at a molecular level in many physiological systems. The chapters are organized into three parts and cover topics such as measuring and modeling physiological and behavioral rhythms;...
Springer, 2019. — 294 p. — (Methods in Molecular Biology 1959). — ISBN: 978-1-4939-9163-1. This volume presents modern and enhanced methods that detail techniques to perform proteomics analyses dedicated to biomarker discovery for human health. Chapters guide readers through pre/post analytical factors, protocols for the preparation of extracellular vesicles and exosomes, and...
Humana Press, 2016 — 544p. — (Methods in Molecular Biology 1377) — ISBN: 978-1-4939-3179-8 (eBook), 978-1-4939-3178-1 (Hardcover).
This volume provides a broad collection of protocols for many of the common experimental techniques used for the characterization of P-type ATPases. P-Type ATPases: Methods and Protocols provides comprehensive practical instructions for all...
2nd Edition. - Humana Press, 2019. - 479 p. - Volume 1921 of Methods in Molecular Biology. The methods described in the second edition on Legionella are for the study of distinct features of L. pneumophila. Chapters guide readers through ecology and physiology of legionella, legionella genetics, cellular microbiology of legionella, biochemical assays to study legionella effectors...
Humana Press, 2013. — 630 p. — (Methods in Molecular Biology 954). Legionnaires’ disease is a severe form of pneumonia that can occur in epidemics of several hundred cases. This atypical pneumonia is characterized by a high mortality rate and affects primarily elderly and immuno-compromised individuals. An in-depth understanding of the ecology and virulence of Legionella spp....
Humana Press, 2015 — 261p. — (Methods in Molecular Biology 1310) — ISBN: 978-1-4939-2690-9 (eBook), 978-1-4939-2689-3 (Hardcover). This volume aims to bring together a variety of protocols useful for DNA-based typing of blood cell antigens. Protocols range from simple approaches with low technical complexity to highly sophisticated modern developments. Written for the Methods...
Humana Press, 2019. - 257 p. - Volume 1941 of Methods in Molecular Biology. This volume aims to provide clear and detailed methods to probe glutamate receptor function. Chapters in this book feature methods to study synaptic ultrastructure, receptor dynamics/receptor interactions and trafficking, cellular plasticity, receptor gene regulation, epigenetics, and clinical...
Humana Press, 1991. - 488 p. ISBN: 0896032299 Series: Methods in Molecular Biology Series , №12 Electrophoretic Techniques. Field-Inversion Gel Electrophoresis. Resolving Multi-megabase DNA Molecules Using Contour-Clamped Homogeneous Electric Fields (CHEF). Separation and Size-Determination of DNA over a 10-200 kbp Range. PFGE Using Double-Inhomogeneous Fields or Orthogonal...
Humana Press, 1993. - 380 p. ISBN: 0896032345 Series: Methods in Molecular Biology, №16 Nucleases: An Overview Deoxyribonuclease I (EC 3.1.21.1) and II (EC 3.1.22.1) DNA Polymerases (EC 2.7.7.7) Taq Polymerase (EC 2.7.7.7): With Particular Emphasis on Its Use in PCR Protocols Eukaryotic Nuclear RNA Polymerases (EC 2.7.7.6) Reverse Transcriptase (EC 2.7.7.49): The Use of Cloned...
Humana Press, 2001. — 452 p. — (Methods in Molecular Biology 43). With the molecular mechanisms underlying the various forms of muscular dystrophy now rapidly clarifying, precise diagnosis has become a reality, and even a requirement in clinical practice. In Muscular Dystrophy: Methods and Protocols, Katherine Bushby and Louise Anderson have assembled an outstanding collection of...
3rd edition. — Humana Press, 2023. — 283 p. — (Methods in Molecular Biology 2677). — ISBN 978-1-0716-3258-1. This third edition volume expands on the previous editions with new discussion on the latest techniques and model systems used to study germline stem cells. The chapters in this book cover topics such as analysis of the C. elegans germline stem cell pool; live imaging of...
Humana Press, 2017. — 276 p. — (Methods in Molecular Biology 1512). Campylobacter jejuni (C. jejuni) is often regarded as the one of the most common causes of bacterial gastroenteritis worldwide. The goal of this volume is to highlight key protocols for working with C. jejuni. In particular, chapters aim to highlight recent developments with regards to in vivo models for C. jejuni...
Humana Press, 2024. — 379 p. — (Methods in Molecular Biology 2717). — ISBN 978-1-0716-3452-3. This detailed volume provides a comprehensive collection of methods and protocols in food allergy and food allergens studies. The selected protocols explore the study of food allergens, from recombinant production, purification procedures, IgE and T cell epitopes characterization, to...
Humana Press, 2024. — 379 p. — (Methods in Molecular Biology 2717). — ISBN 978-1-0716-3453-0. This detailed volume provides a comprehensive collection of methods and protocols in food allergy and food allergens studies. The selected protocols explore the study of food allergens, from recombinant production, purification procedures, IgE and T cell epitopes characterization, to...
Humana Press, 2016 — 243p. — (Methods in Molecular Biology 1370) — ISBN: 978-1-4939-3142-2 (eBook), 978-1-4939-3141-5 (Hardcover). This volume aims to present a large panel of techniques for the study of Plant Cell Division. Plant Cell Division: Methods and Protocols captures basic experimental protocols that are commonly used to study plant cell division processes, as well as...
Humana Press, 2016. — 240 p. — (Methods in Molecular Biology 1356).
This volume provides detailed discussion of a variety of important techniques that researchers use to study fungal molecular biology and pathogenesis. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents,...
2nd edition. — Humana Press, 2022. — 388 p. — (Methods in Molecular Biology 2542). — ISBN 978-1-0716-2548-4. This updated volume explores Candida and candidiasis methods, useful to a wide variety of Candida scientists including those new to the field. Beginning with a section on the Candida genome, the book continues by covering techniques for antifungal discovery and studying...
Humana Press, 2018. — 447 p. — (Methods in Molecular Biology 1709). This volume is a compilation of laboratory protocols and methodology required for the study of molecular chaperones and the cellular stress response. Chapters detail stress response in Hsf1, Hsf2 and Hsf4 knockout mice, mapping HSP interaction networks, the LUminescence-based Mammalian IntERactome (LUMIER), Hsp70...
2nd edition. — Humana Press, 2023 — 328 p. — (Methods in Molecular Biology 2693). — ISBN 978-1-0716-3341-0. This second edition volume expands on the previous edition with new discussions on the latest techniques used to study molecular chaperones and the stress response. The chapters in this book cover such as analysis of the initiation and regulation of the stress response;...
New York: Humana Press, 2022. — 394 p. This volume provides up-to-date methods on single cell wet and bioinformatics protocols based on the researcher experiment requirements. Chapters detail basic analytical procedures, single-cell data QC, dimensionality reduction, clustering, cluster-specific features selection, RNA velocity, multi-modal data integration, and single cell RNA...
Humana Press, 2014. — 289 p. Series: Methods in Molecular Biology, № 1148 The book focuses on proven applications of photoswitching proteins in cell and neurobiology. Optical approaches have been mostly embraced by the neurosciences to allow fast, high-resolution characterization and manipulation of single cells in complex neuronal tissue. These tools, including photoswitching of...
Humana Press, 2016. — 305 p. — (Methods in Molecular Biology 1445). — ISBN 978-1-4939-3716-5. This volume provides readers with a wide collection of the latest and readily reproducible technical protocols available in the field of non-viral gene delivery vectors. The chapters in this book are organized into three major parts: Part I is a section on conventional bolus gene...
Humana Press, 2021. — 351 p. — (Methods in Molecular Biology 2254). — ISBN 978-1-0716-1157-9. This detailed volume presents a comprehensive bioinformatic and experimental toolbox for prioritizing, annotating, and functionally analyzing long non-coding RNAs (lncRNAs). Playing a vital role in diverse biological progresses and human disease, lncRNAs have proven to be a challenging...
Humana Press, 2016 — 332p. — (Methods in Molecular Biology 1406) — ISBN: 978-1-4939-3444-7 (eBook), 978-1-4939-3442-3 (Hardcover). This volume provides resources, ideas, and bench manuals for the study of breast cancer. This book is divided into five sections: methods used in clinical laboratory for diagnosis (Detection of Molecular Markers of Breast Cancer); methods used in...
Humana Press, 2013. — 243 p. Series: Methods in Molecular Biology, № 1013 Chemokines constitute a large family of structurally similar cytokines that contain a signature of conserved cysteine residues joined by disulfide bridges. Binding of chemokines to specific G protein-coupled receptors followed by downstream signaling defines their biological function. Initially,...
New York: Humana Press, 2015. — 186 p. The book offers a collection of methods for those interested in the discovery, localization, and functional analysis of these non-coding transcripts that have the potential and ability to orchestrate and control gene expression. After a review of the field, this detailed volume continues with methods useful for the study of siRNAs, microRNAs...
Humana Press, 2005. — 352 p. — (Methods in Molecular Biology 309). — ISBN: 1-59259-935-4. Identification of Components of RNAi Pathways Using the Tandem Affinity Purification Method Separation of Drosophila RNA Silencing Complexes by Native Gel Electrophoresis Preparation and Analysis of Drosha RNA Interference in Caenorhabditis elegans Down-Regulating Gene Expression by RNA...
Humana Press, 2004. — 326 p. Series: Methods in Molecular Biology, № 274 A comprehenive and cutting-edge collection of basic and advanced biochemical techniques for isolating, treating, and analyzing the photosynthetic materials and processes. All protocols follow the successful Methods in Molecular Biology series format, each one offering step-by-step laboratory instructions,...
Humana Press, 2013. — 554 p. Series: Methods in Molecular Biology, № 927 Semen analysis methods. Tests of sperm function. Sperm DNA damage testing. Advanced clinical testing. Sperm preparation and selection techniques. Staging and histology techniques. Oxidative stress testing. Analytical tools and methods. Methodologies to evaluate sperm epigenetics. In vitro and ex vivo...
Humana Press, 2014 — 347p. — (Methods in Molecular Biology 1128) — ISBN: 978-1-62703-974-1 (eBook), 978-1-62703-973-4 (Hardcover). In Developmental Biology of the Sea Urchin and Other Marine Invertebrates: Methods and Protocols, expert researchers in the field detail many of the methods which are now used to study sea urchins and other marine invertebrates in the laboratory....
Humana Press, 2010.— 407 p. Series: Methods in Molecular Biology, ISBN: 978-1603272407, e-ISBN: 978-1603272414. Whereas getting exact data about living systems and sophisticated experimental procedures have primarily absorbed the minds of researchers previously, the development of high-throughput technologies has caused the weight to increasingly shift to the problem of...
3rd edition. — Humana Press, 2022.— 391 p. — (Methods in Molecular Biology 2449). — ISBN 978-1-0716-2094-6. This third edition details new and updated methods and protocols on important databases and data mining tools. Chapters guides readers through archives of macromolecular sequences and three-dimensional structures, databases of protein-protein interactions, methods for...
Humana Press, 2017. — 221 p. — (Methods in Molecular Biology 1582). While HIV-1 continues to be well-researched, this detailed volume draws attention to other members of the Retrovirus family, namely the Human T-lymphotropic Viruses (HTLVs), featuring the most updated technical information about HTLV determination and the methods to investigate their interaction with the host...
Humana Press, 2015 — 439p. — (Methods in Molecular Biology 1321) — ISBN: 978-1-4939-2760-9 (eBook), 978-1-4939-2759-3 (Hardcover).
Conceived with the intention of providing an array of strategies and technologies currently in use for glyco-engineering distinct living organisms, this book contains a wide range of methods being developed to control the composition of...
Humana Press, 2019. — 420 p. This volume provides a complete and timely guide to the use of adeno-associated virus (AAV) vectors for genetic manipulation of mammalian tissues. Beginning with methods for the design and characterization of AAV vectors, the book continues with protocols for AAV delivery to various components of the central nervous system, to a number of sensory...
Humana: 2024. — 299 p. This detailed volume collects techniques to study the highly regulated cell cycle process. Beginning with chapters investigating these processes and assessing how cells respond when these complicated pathways are simplified by using synthetic biology and in vitro reconstitutions, the book continues by exploring how cells sense and respond to environmental...
Springer, 2009. — 211 p. — (Methods in Molecular Biology 551). — ISBN 978-1-60327-998-7. The development of molecular genotyping methods has revolutionized the possibility for classification of microorganisms at the sub-species level. This investigation of species diversity is crucial for deciding the molecular relatedness of isolates for epidemiological studies. In Molecular...
Totowa, New Jersey: Humana Press, 2008. — 334 p. — (Methods in Molecular Biology, № 454). — ISBN: 1588298671. In 2003, the word "coronavirus" spread across the globe, somewhat further than the virus that sparked the panic. In SARS- and Other Coronaviruses: Laboratory Protocols, expert researchers examine these devastating viruses through detailed laboratory protocols. Chapters...
Humana Press, 2021. — 326 p. — ISBN 978-1-0716-1640-6. This thorough book collects methods and strategies to analyze proteomics data. It is intended to describe how data obtained by gel-based or gel-free proteomics approaches can be inspected, organized, and interpreted to extrapolate biological information. Organized into four sections, the volume explores strategies to...
Humana Press, 2021. — 326 p. — ISBN 978-1-0716-1640-6. This thorough book collects methods and strategies to analyze proteomics data. It is intended to describe how data obtained by gel-based or gel-free proteomics approaches can be inspected, organized, and interpreted to extrapolate biological information. Organized into four sections, the volume explores strategies to...
Humana Press, 2012. — 269 p. — (Methods in Molecular Biology 825). The germline is unique in mammals as it is the only cell lineage that undergoes mitosis, meiosis, and differentiation, making these cells amenable to in-depth genomic and mechanistic studies. In this volume expert researchers provide a wide-ranging collection of techniques that can be applied to the study of...
Humana Press, 2022. — 528 p. — (Methods in Molecular Biology 2438). — ISBN 978-1-0716-2034-2. This volume explores detailed methods to investigate various aspects of biology related to cell polarity, or asymmetry within a cell. Molecular, cellular, and tissue-level regulation and function as well as diseases caused by impairment of cell polarity are explored by these methods....
New York: Humana Press, 2011. — 276 p. The numerous vital applications of complementary DNA (cDNA) technology have changed dramatically as the technology has advanced over recent years. In cDNA Libraries: Methods and Protocols, expert researchers provide current techniques that reflect the latest advances in the construction and application of cDNA libraries. The first half of...
Humana, 2021. — 199 p. This detailed book focuses on various aspects of Invariant Natural Killer T-Cells (iNKT), which are known to contribute to homeostasis and autoimmunity and can also cause various pathological responses such as allergy, infection, excessive autoimmune response, and cancer. The volume explores techniques for identification and isolation of iNKT cells, iNKT...
Humana press, 1996. — 344 p. Series: Methods in Molecular Biology, № 61 The purpose of the preface is to explain the book's objectives and how to use it; give warnings, disclaimers, and the like.* The main objective of Protein and Peptide Analysis by Mass Spec trometry is quite straightforward—to present authoritative, up-to-date, and practical accounts of the use of mass...
2nd edition. — Springer, 2021. — 363 p. — (Methods in Molecular Biology 2199). — ISBN: 978-1-0716-0891-3. This updated edition emphasizes the benefits of structural genomics to the wider structural research community with a diverse range of applicable methods. The book opens with an extensive section on protein production, including new methodologies for membrane and...
ITexLi, 2023. — 138 p. — ISBN 180356816X 9781803568164 1803568151 9781803568157 1803568178 9781803568171. CRISPR technology has been extensively used in vitro and in vivo as a tool in basic research for genetic editing (e.g., genome encoding, silencing, enhancing, and modification). Although there are many technical and ethical challenges to overcome, such as off-target...
Humana Press, 2014. — 247 p. — ISBN: 1627036784 Series: Methods in Molecular Biology, №1089 Reflecting the development of powerful new tools and high-throughput methods to analyze adenoviral particles and their interactions with host cells, the third edition of Adenovirus Methods and Protocols calls upon experts in the field to convey advances in molecular biology, genomics and...
Humana Press, 1994. - 476 p. ISBN: 9780896032804 Series: Methods in Molecular Biology, №33 Preparation of Human Chromosomal Painting Probes from Somatic Cell Hybrids. Reverse Chromosome Painting. FISH Detection on DAPI Banded Chromosomes. Generation of Alphoid DNA Probes for Fluorescence In Situ Hybridization (FISH) Using the Polymerase Chain Reaction. In Situ Hybridization...
Humana Press, 2012. - 690 p. ISBN: 161779841X Series: Methods in Molecular Biology, №882 The HLA molecules are important regulators of the immune response through mediating antigen presentation and interaction between key immune mediating cells. They are also the major histocompatibility barriers to transplantation, which is the clinical paradigm of the self versus non self...
Humana Press, 2019. — 468 p. — (Methods in Molecular Biology 1997). — ISBN: 978-1-4939-9495-3. This detailed book addresses the continual development and application of new methods and protocols to understand the biology of the pathogen Neisseria gonorrhoeae. Beginning with gonococcus biology and antimicrobial resistance, the volume continues with areas of study such as...
Humana Press, 2022. — 1081 p. — (Methods in Molecular Biology 2452). — ISBN 978-1-0716-2111-0. This detailed volume provides the increasing number of SARS-CoV-2 researchers with a useful handbook covering multidisciplinary approaches on various aspects of SARS-CoV-2 research, brought together by leading laboratories across the globe. Topics covered include techniques in...
Humana Press, 2022. — 479 p. — (Methods in Molecular Biology 2452). — ISBN 978-1-0716-2110-3. This detailed volume provides the increasing number of SARS-CoV-2 researchers with a useful handbook covering multidisciplinary approaches on various aspects of SARS-CoV-2 research, brought together by leading laboratories across the globe. Topics covered include techniques in clinical...
Humana Press, 1995. — 398 p. ISBN: 0896033236 Series: Methods in Molecular Biology, №50 University of York, U.K. Manual of protocols in the identification of many organisms using nucleic acid analysis: plant, insect, and vertebrate DNA; human and animal viruses; bacteria; fungi; protozoans; parasites; and mosquitoes.
Humana Press, 2010. — 2nd Ed. — 472 p. — (Methods in Molecular Biology 622). — ISBN: 978-1-60327-299-5 (eBook), 978-1-60327-298-8 (Hardcover). Since the discovery of a collagen-degrading protease in the tadpole tail in 1962, matrix metalloproteinase research has led to the discovery of more than twenty distinct vertebrate MMPs, along with a variety of homologues from diverse...
Humana Press, 2012. - 250 p. - Methods in Molecular Biology, v 818. - SELDI is distinct from other TOF-MS technologies in that it couples features of chromatography and mass spectrometry, facilitating analyte enrichment and sample cleanup on an array surface. In the growing field of proteomics, SELDI technology has been widely used for biomarker discovery and characterization...
Humana Press, 2017. — 461 p. — (Methods in Molecular Biology 1559). This volume presents a broad selection of cutting-edge methods and tools that will enable the reader to investigate the multi-faceted manifestations of inflammation. Inflammation: Methods and Protocols is divided into four sections: the first three sections describe protocols investigating immune-mediated...
Humana Press, 2009. — 313 p. — (Methods in Molecular Biology 501) — ISBN: 978-1-58829-682-5. Ranging from the evolution of pathogenicity to oceanic carbon cycling, the many and varied roles that bacteriophages play in microbial ecology and evolution have inspired increased interest within the scientific community. Bacteriophages: Methods and Protocols pulls together the vast...
Humana Press, 2021. — 1058 p. — (Methods in Molecular Biology 2185). — ISBN 978-1-0716-0810-4. The detailed volume aims to provide a comprehensive hands-on manual covering all the techniques involved in the cellular and molecular identification and characterization of both normal hematopoietic and leukemic stem cells, both from human patients and from mouse models of human...
New York: Humana Press, 2023. — 217 p. This second edition details new and updated chapters on key methodologies and breakthroughs in the mass spectrometry imaging (MSI) field. Chapters guide readers through nano-Desorption Electrospray Ionisation (nDESI), Matrix Assisted Laser Desorption Ionisation-2 (MALDI-2), Laser Ablation - Inductively Coupled Plasma-Mass Spectrometry...
Humana Press Inc., 2006. — 306 p. — (Methods in Molecular Biology 341). — ISBN: 1588295230. A versatile collection of readily reproducible cell-cell interaction assays for uncovering cellular interactions at the molecular level, both in vitro and in vivo. The protocols cover a diverse set of cell-cell interaction models in both normal and pathological states, are readily...
Humana Press, 1991. - 316 p. ISBN: 089603299X Series: Methods in Molecular Biology, №8 The Retroviral Life Cycle and the Molecular Construction of Retrovirus Vectors Construction of Retroviral Packaging Cell Lines Making High Titer Retroviral Producer Cells Methods of Titrating Nonselectable Recombinant Retroviruses Selectable Eukaryotic-Cell Markers Virus Genome Detection by...
Humana Press, 1991. - 458 p. ISBN: 0896032051 Series: Methods in Molecular Biology, №9 Protocols in Human Molecular Genetics highlights the tremendous advances in our ability to work on the human genome that have emerged in the past few years. The latest techniques are set forth in the clear, concise, easy-to-follow format that is the hallmark of Humana's Methods in Molecular...
Humana Press, 1999. - 412 p. Series: Methods in Molecular Biology, №121 Cloning Human Natural Killer Cells Generation of Short-Term Murine Natural Killer Cell Clones to Analyze Ly49 Gene Expression Cloning and Culturing of Fetal Mouse Natural Killer Cells Techniques for Studying Development of Human Natural Killer Cells and T Cells Murine Natural Killer Cell Cloning from Fetal...
Humana Press, 2016. - 309 p. - ISBN: 978-1-4939-3668-7 Series: Methods in Molecular Biology, №1435 Protocols for West Nile Virus Research is a collection of research methods, techniques, and approaches to investigate the molecular biology of West Nile virus (WNV). The target audience is comprised of researchers such as virologists, molecular biologists, microbiologists, and...
Humana Press, 2017. — 375 p. — (Methods in Molecular Biology 1550). This volume aims to provide protocols on a wide range of biochemical methods, analytical approaches, and bioinformatics tools developed to analyze the proteome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the...
Humana Press, 2014. — 317 p. Series: Methods in Molecular Biology, № 1153 The book is a collection of detailed techniques that will be a useful tool for a wide range of plant biologists, as well as for scientists of other fields interested in plant isoprenoids. Isoprenoids are an incredibly diverse family and they participate in a large variety of processes. Divided into four...
Humana Press, 2012. - 278 p. ISBN: 162703112X The discovery of catalytic RNAs in the mid-1980s marked the beginning of a new era in RNA biology and an ever increasing appreciation of the diverse and critical roles played by this fascinating molecule. In Recombinant and In Vitro RNA Synthesis: Methods and Protocols, expert researchers in the field detail approaches from the...
Humana Press, 2023. — 423 p. — (Methods in Molecular Biology 2685). — ISBN 978-1-0716-3294-9. This volume focuses on the latest techniques used in forensic DNA analysis. The chapters include a comprehensive collection of extraction, quantification, STR amplification, and detection methods for routine forensic samples, including manual, semi-automated, and automated procedures...
Humana Press, 2023. — 423 p. — (Methods in Molecular Biology 2685). — ISBN 978-1-0716-3295-6. This volume focuses on the latest techniques used in forensic DNA analysis. The chapters include a comprehensive collection of extraction, quantification, STR amplification, and detection methods for routine forensic samples, including manual, semi-automated, and automated procedures...
Humana Press, 2025. — 225 p. Preface Contributors An Overview Thyroid Hormones and Co-workers: An Overview Thyroid Hormone Action by Genomic and Nongenomic Molecular Mechanisms Thiroid Hormones A Sensitive Radioimmunoassay for T3 and T4 Determinations in Plasma and Tissues Generation of a Mouse Model for the Study of Thyroid Hormones Regulatory Effect on the Immune System...
Humana Press, 2010. — 229 p. — (Methods in Molecular Biology). — ISBN10: 1617371173, 13 978-1617371172. Amino acid analysis is a technique that has become commonplace in biotechnology, biomedical, and food analysis laboratories. This book describes a variety of amino acid analysis techniques and how each technique can be used to answer specific biological questions. The first...
Humana Press, 2002. — 414 р. — (Methods in Molecular Biology, № 197). — ISBN: 0-89603-972-2. Mutations within mitochondrial DNA (mtDNA) and the nuclear genes involved in the maintenance of mitochondrial DNA have been linked to a wide range of human diseases, including several of the most common diseases of aging. In Mitochondrial DNA: Methods and Protocols internationally...
Humana Press, 2000. — 418 p. Series: Methods in Molecular Biology, № 125 Anthony P. Corfield and a group of hands-on expermentalists bring together in this volume a state-of-the-art collection of reliable and tested methods for the study of all aspects of mucins. These powerful methods range from the preparation of mucins to their detection and quantitation with molecular...
Humana Press, 2022. — 273 p. — (Methods in Molecular Biology 2420). — ISBN 978-1-0716-1935-3. This detailed volume explores techniques in clinical proteomics, an emerging discipline aimed at deciphering the molecular mechanisms underlying the progression of diseases and at identifying new biomarkers and therapeutic targets to expand the physicians’ toolbox for precision...
Humana Press, 2007. — 240 p.
Series: Methods in Molecular Biology, № 370
The second edition of Adhesion Protein Protocols combines traditional techniques with cutting-edge and novel techniques that can be adapted easily to different molecules and cell types. The topics discussed include novel techniques for studying cell-cell adhesion, neutrophil chemotaxis, in vitro assays...
New York: Humana Press, 2016. - 349 p. Series: Methods in Molecular Biology, № 1342 This volume brings together a unique collection of protocols that cover standard, novel, and specialized techniques. Cell Cycle Oscillators: Methods and Protocols guides readers through recent progress in the field from both holistic and reductionist perspectives, providing the latest...
Humana Press, 2018. - 356 p. - Volume 1770 of Methods in Molecular Biology. This user-friendly book provides a range of classical and modern techniques for the study of photosynthesis in a manner accessible to a broad spectrum of researchers. Broken into four sections, it explores the measurement of physiological photosynthetic parameters, quantifying photosynthetic enzyme...
Humana Press, 1997. — 332 p. Series: Methods in Molecular Biology, № 69 The first libraries of complementary DNA (cDNA) clones were con structed in the mid-to-late 1970s using RNA-dependent DNA polymerase (reverse transcriptase) to convert poly A* mRNA into double-stranded cDNA suitable for insertion into prokaryotic vectors. Since then cDNA technology has become a fundamental...
Humana Press, 2012. — 416 p. — (Methods in Molecular Biology 854). Protein analysis is increasingly becoming a cornerstone in deciphering the molecular mechanisms of life. Proteomics, the large-scale and high-sensitivity analysis of proteins, is already pivotal to the new life sciences such as Systems Biology and Systems Medicine. Proteomics, however, relies heavily on the past...
Humana Press, 2013. — 262 p. Series: Methods in Molecular Biology, № 1003 Despite the best efforts of many and despite landmark discoveries and experimental ingenuity, challenges in the pursuit of research related to olfactory receptors (ORs) continue to exist. In this volume, experts in the field contribute chapters that serve to address these challenges. The volume consists...
Humana Press, 2007. — 260 p.
Series: Methods in Molecular Biology, № 389
The use of Pichia Pastoris as a recombinant protein system has increased greatly in popularity over the years. In Pichia Protocols 2nd Edition, the author focuses on new information that has come to light in recent years but repeats key basic methods so that researchers are not absolutely dependent on...
Humana Press, 2016. — 298 p.
Series: Methods in Molecular Biology №1352.
This expert volume provides insights into the technological fundamentals together with a comprehensive overview of the potentialities of peptide microarray technology in basic research and clinical assays. Advancements made in recent years in peptide library synthesis, immobilization chemistry and array...
3rd edition. — Humana Press, 2023. — 261 p. — (Methods in Molecular Biology 2578). — ISBN 978-1-0716-2731-0. This detailed volume provides an updated overview of current uses of peptide microarray technology, showcasing consolidated applications while highlighting some of the most intriguing novelties and emerging fields of use. The methodologies within this collection are of...
Humana Press, 1995. — 214 p. — (Methods in Molecular Biology 42). — ISBN: 0896032795. Basic Immunology Basic Principles of ELISA Stages in ELISA Theoretical Considerations Direct ELISA Titration of Antigen and Antibody Indirect ELISA Use of Antibodies on Solid-Phase in Capture ELISA Competitive ELISA Immunochemical Techniques
Second Edition. — Humana Press, 2009. — 574 p. Series: Methods in Molecular Biology, №516 The Enzyme Linked Immunosorbent Assay (ELISA) continues, as predicted, to be the predominant assay used in biomedical and agricultural research. While little has changed in the principles of the ELISAs used since the publication of The ELISA Guidebook, many technological advantages,...
Humana Press, 1995. — 223 p. — ISBN: 0-89603-279-5. ELISA: Theory and Practice introduces to scientists at all levels of expertise the principles of the most commonly used assay technique known as the Enzyme Linked Immunosorbent Assay. The book provides readers with full descriptions of the basic systems that make ELISA one of the most powerful techniques in science today, and...
Humana Press, 2018. — 269 p. — (Methods in Molecular Biology 1786). — ISBN 978-1-4939-7843-4. This volume presents methods and protocols on modern analysis of steroid receptor and prostate cancer function. Chapters detail androgen, androgen receptor action, anti-androgen enzalutamide, in vitro and in vivo models, imaging, gene fusions, and help facilitate studies with novel...
Humana Press, 2010. — 290 p. Series: Methods in Molecular Biology, № 599. Since its first systematic application during the 1970s, bioremediation, or the exploitation of a biological system’s degradative potential to combat toxic pollutants such as heavy metals, polyaromatic hydrocarbons (PAH), cyanides, and radioactive material, has proven itself over time, and the many...
Humana Press, 2011 — 473p. — (Methods in Molecular Biology 677) — ISBN: 978-1-60761-869-0 (eBook), 978-1-60761-868-3 (Hardcover). Over the past several years, a high diversity of regulatory cells and suppressive molecules has taken centre stage in the field of immunoregulation.In Suppression and Regulation of Immune Responses: Methods and Protocols, expert researchers highlight...
Humana Press, 2016 — 260p. — (Methods in Molecular Biology 1371) — ISBN: 978-1-4939-3139-2 (eBook), 978-1-4939-3138-5 (Hardcover).
This second volume expands upon the previous edition with new research and objectives in immunoregulation and immune tolerance. Chapters cover topics ranging from new molecular and cellular mechanisms of tolerance; generation and characterization of...
Humana Press, 2003. — 269 p. Series: Methods in Molecular Biology, № 239 Cell migration is now well recognized as a critical component of the inflammatory disease process, so that its proper understanding promises to generate both ground-breaking basic discoveries and the development of novel therapeutics. In this volume leading cell biologists and immunologists present their most...
Humana Press, 2019. — 242 p. — (Methods in Molecular Biology 1919). This volume details various technological advances to isolate, perpetuate, and characterize neural stem cells from various sources and formulates them for the delivery into animal models of neurological disorders. Chapters guide the reader through methods and protocols on definition and isolation of neural stem...
Springer, 2023. — 266 p. This book provides protocols on the preparation of synaptic vesicles from different model systems and provides instructions for functional investigations of their transport and formation, scaffolding, filling and priming, release, recycling, and degradation. Chapters guide readers through computational approaches, simulating different aspects of...
3rd edition. — New York: Humana Press, 2022. — 423 p. This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and metabolism, recent...
Humana Press, 2008. — 438 p. — (Methods in Molecular Biology).
Drosophila is a comprehensive collection of methods and protocols for Drosophila, one of the oldest and most commonly used model organisms in modern biology. The protocols are written by the scientists who invented the methods. The text presents a diverse set of techniques that range from the basic handling of flies...
Humana Press, 2021. — 333 p. — (Methods in Molecular Biology 2188). — ISBN: 978-1-0716-0817-3. This volume describes a range of standard and novel methodological approaches used to probe ion channel function across different modalities. Chapters guide readers through methods and protocols from an introduction to the decades old patch clamp method for the ion channel neophyte to...
Humana Press, 2017. — 305 p. — ISBN: 1493968645. Series: Methods in Molecular Biology, N 1580 This volume is a collection of miRNA detection and target identification protocols, detailing new developments in the traditional detection approaches such as northern blot, quantitative real-time PCR, array, next generation sequencing, and in situ hybridization. The chapters in...
2nd edition. — Humana Press, 2023. — 267 p. — (Methods in Molecular Biology 2630). — ISBN 978-1-0716-2981-9. This updated volume reflects new and evolved techniques to study detection, profiling, and manipulation of microRNAs (miRNAs) in plants and animals. After overviews of how best to detect, identify, and validate microRNAs, the book continues by exploring state-of-the-art...
Springer, 2021. — 218 p. — ISBN: 1071608444. This detailed book explores a diverse range of topics related to wound healing. Some areas include wound generation as a tool to study cellular interactions and growth, mechanisms by which different tissues regenerate, ischemic wounds and their treatment, repairing corneal wound, as well as a number of emerging technologies to help...
3rd edition. — Humana Press, 2022. — 412 p. — (Methods in Molecular Biology 2404). — ISBN 978-1-0716-1850-9. This volume presents the most recent advances in techniques for studying the post-transcriptional regulation of gene expression (PTR). With sections on bioinformatics approaches, expression profiling, the protein and RNA interactome, the mRNA lifecycle, and RNA...
3rd edition. — Humana Press, 2022. — 905 p. — (Methods in Molecular Biology 2404). — ISBN 978-1-0716-1851-6. This volume presents the most recent advances in techniques for studying the post-transcriptional regulation of gene expression (PTR). With sections on bioinformatics approaches, expression profiling, the protein and RNA interactome, the mRNA lifecycle, and RNA...
Humana Press, 2010. — 324 p. — (Methods in Molecular Biology, vol.626). — ISBN-10: 1607615843, ISBN-13: 978-1607615842. Product Description Spanning from discoveries in fundamental immunology to industrial and commercial concerns, the study of vaccine adjuvants has developed into an exciting area of work with great, vital potential in innovating techniques in which adjuvants...
Humana Press, 1995, 260 p. ISBN: 0896033082 Series: Methods in Molecular Biology, №45 Methods of Immunization to Enhance the Immune Response to Specific Antigens In Vivo in Preparation for Fusions Yielding Monoclonal Antibodies. Methods of Immunization to Enhance the Immune Response to Specific Antigens In Vitro. Culture Conditions that Optimize Outgrowth of Hybridomas....
Humana Press, 2014. — 431 p. — (Methods in Molecular Biology 1180). — ISBN: 978-1-4939-1049-6. Histopathology: Methods and Protocols provides a comprehensive guide to the current issues in histopathology. With chapters on organ-based approaches with specific protocols for morphologic, molecular examination and pathological observations governing the therapeutic management of...
Humana Press, 1995. - 240 p. ASIN B0019K5V18 Series: Methods in Molecular Biology, №38 Overview Virus Cryopreservation and Storage Freeze-drying and Cryopreservation of Bacteria Freeze-drying of Yeasts Cryopreservation of Yeasts Cultures Cryopreservation and Freeze-drying of Fungi Cryopreservation of Pathogenic and Nonpathogenic Free-Living Amoebae Long-Term Cryopreservation of...
2nd Edition. - Humana Press, 2007. - 362 p. - Volume 368 of Methods in Molecular Biology. - Cryopreservation and Freeze-Drying Protocols: Second Edition is a compilation of robust, reproducible techniques for the conservation of a wide range of biological materials. This widely expanded second edition includes novel approaches and protocols for biological materials that were not...
Springer, 2019. — 224 p. — (Methods in Molecular Biology 1913). – ISBN10: 1493989782. This detailed volume describes a series of techniques that are essential for evaluating the efficacy of new checkpoint blockade therapies as well as understanding the mechanisms behind the therapies that have already been approved. Beginning with a section on describing the tumor...
Humana Press, 2019. - 357 p. - Volume 1932 of Methods in Molecular Biology. This detailed volume provides a collection of protocols for the study of miRNA functions in plants. Beginning with coverage of miRNA function, biogenesis, activity, and evolution in plants, the book continues by guiding readers through methods on the identification and detection of plant miRNAs,...
Humana, 2009. — 316 p. Protein phosphorylation controls many basic cellular processes, such as cell growth, differentiation, migration, metabolism, and cell death, and its study can provide key insights into the signal transduction pathways that are activated in cells in response to different stimuli, such as growth factor stimulation or exposure to toxicants. In...
Humana Press, 2019. — 411 p. — (Methods in Molecular Biology 2075) — ISBN: 978-1-4939-9876-0 This book focuses on technologies used to study horizontal gene transfer (HGT) in prokaryotes. Beginning with a section on the detection and isolation of mobile genetic elements (MGEs), the volume continues with sections concentrating on the analysis of conjugation, transformation, and...
Humana Press Inc., Totowa, NJ. - 2003. -248 p. Methods in Molecular Biology, vol. 249 A collection of biochemical, cellular, and molecular techniques for unraveling and quantifying the events occurring between the initial contact of a cytokine at the membrane receptor and the eventual activation of gene transcription. The techniques used include the generation of transfectants,...
Humana Press, 2017. — 257 p. — (Methods in Molecular Biology 1544). This volume provides detailed techniques used for the study and characterization of the plant vascular system, with a central focus on the xylem tissue. Chapters are organized in three main sections covering; analysis of xylem development, xylem characterization though imaging techniques, and analysis of the xylem...
Humana Press, 2010. - 287 p. Methods in Molecular Biology, Vol 627 While commercial instruments have expanded the usage and the related literature has increased, the quality of surface plasmon resonance (SPR) research has been hindered by a lack of knowledge of the processes that influence the SPR signal. In Surface Plasmon Resonance: Methods and Protocols, experts in the field...
Humana Press, 2013. — 228 p. Series: Methods in Molecular Biology, № 1040 Inflammasomes have a seemingly simple architecture. A sensor molecule of the NLR or PYHIN protein family recruits the common adapter molecule apoptosis-associated speck-like protein containing a CARD (ASC). Even so, inflammasome activation is highly regulated at multiple steps and precise details of how...
Humana, 2021. — 333 p. This detailed book provides a comprehensive series of innovative research techniques and methodologies applied to the parasite genomics research area, all applying different approaches to analyzing parasite genomes and furthering the study of genetic complexity and the mechanisms of regulation. Beginning with chapters on novel sequencing and the...
New York: Humana Press, 2023. — 514 p. This volume provides protocols on evidence for polyploidy and how it can be unveiled. Chapters guide readers through evolutionary experiments, measure effects of polyploidy, evidence for (remnants of) ancient WGDs, models of chromosome number evolution, population genomics approaches to study polyploidy, analysing genetic data from...
2nd Edition. — Humana Press, 2014. — 607 p. Series: Methods in Molecular Biology, №1184 At the intersection of experimental and computational sciences, the second edition of Immunoinformatics provides biological insights as well as a simpler way to implement approaches and algorithms in the immunoinformatics research domain. After an introductory section, this extensive volume...
Humana Press, 2013. — 373 p. Series: Methods in Molecular Biology, № 959 Organogenesis entails the regulation of cell division, cell expansion, cell and tissue type differentiation, and patterning of the organ as a whole. It is essential to gain insight into how organs are initiated and how they develop. However, this very often is subject to technical difficulties as these...
2nd Edition. — Humana Press, 2013. — 238 p. — ISBN: 978-1-62703-235-3 Series: Methods in Molecular Biology, № 962 We are proud to present the second edition of p53 protocols to keep up with the progress in p53 research since the publication of its fi rst edition. The present edition includes 19 chapters delineating state-of-the art methods to address the challenging questions of...
2nd ed. — Humana Press, 2025. — 292 p. This second edition volume expands on the previous edition with new chapters and updated discussions on the latest advancements in the development and practice of metabolic phenotyping. The chapters in this book cover topics such as quality control in untargeted metabolic phenotyping; implementation of quality assurances processes; careful...
Humana Press, 2015 — 275p. — (Methods in Molecular Biology 1293) — ISBN: 978-1-4939-2519-3 (eBook), 978-1-4939-2518-6 (Hardcover). The identification of normal and breast cancer stem cells has offered a new vision of this heterogeneous disease and new hopes for its prognosis and treatment. This volume provides an overview of recent developments in mammary stem cell research and...
Humana Press, 2022. — 284 p. — (Methods in Molecular Biology 2422). — ISBN 978-1-0716-1947-6. This volume provides a comprehensive reference guide for researchers to study the applications of labeled antibodies. Chapters guide reader through the the and practice of immunohistochemistry, immunocytochemistry and immunofluorescence techniques. Written in the highly successful...
Humana Press, 2013. — 402 p. Series: Methods in Molecular Biology, № 966 In recent years, molecular microbiology has emerged as a top, cutting-edge biological discipline, thanks to the multi-disciplinary and integrative approaches taken by investigators seeking to understand the intricacies of the microbial world and how it affects human health and the biosphere. In this...
New York: Springer, 2022. — 229 p. This updated edition collects cutting-edge techniques used to study neural stem and progenitor cells as well as the brain microenvironment. Featuring a wide range of technological advances in the study of neural stem cells, the volume highlights the promises of stem cell-based therapeutic applications for central nervous system ailments....
Springer, 2012. — 292 p. 77 illus., 18 illus. in color. — ISBN: 1627031065, 9781627031066 Series: Methods in Molecular Biology, №939 The post-genomic revolution is witnessing the generation of petabytes of data annually, with deep implications ranging across evolutionary theory, developmental biology, agriculture, and disease processes. Data Mining for Systems Biology: Methods...
Humana Press, 2015. — 278 р. — ISBN10: 1493920197, ISBN13: 978-1493920198 Methods in Molecular Biology, №1248 This volume provides an overview of modern and emerging methods for production, analysis, and utility of peptide libraries. Chapter focus on methods and techniques for synthesis, genetic expression, hybrid synthesis-expression, examples of modern utility of these...
Humana Press, 2013. — 460 p. Series: Methods in Molecular Biology, № 1044 Genetic toxicology is recognized by geneticists and researchers concerned with the genetic impact of man-made chemicals. In this volume expert researchers in the field provide comprehensive genetic toxicology protocols. These include in vitro and in vivo protocols on mutation assays, cytogenetic techniques,...
2nd ed. — Humana Press, 2024. — 341 p. This second edition volume expands on the previous edition with updated chapters covering a broader range of tissues and techniques pertaining to stem cell technologies. The chapters also cover topics such as the generation of iPSC-derived cells unique to the individual human genome addressing the possibility of more personalized clinical...
Humana Press, 2017. - 320 p. Volume 1553 of Methods in Molecular Biology. This volume contains a collection of protocols from some of the major laboratories involved in stem cell research across the world. The research discussed in this book covers topics such as: isolating, characterizing and expanding dental pulp stem cells; manipulating the proliferative potential of...
Springer, 2016. — 257 p. — (Methods in Molecular Biology 1417). — ISBN 978-1-4939-3564-2. This volume provides a sound basis for the molecular investigation of NLR function in health and disease. Chapters focus on of innate immune receptors, “atypical” inflammasomes, biochemical and novel bioluminescence techniques for the measurement of IL-1b, bioluminescent probe, biochemical...
Humana Press, 2012. - 419 p. ISBN: 1627030883 Series: Methods in Molecular Biology, №938 Phytoplasmas are a group of bacteria that are capable of multiplying and causing severe diseases in a wide range of plant species and are transported between plants by insect vectors in which the bacteria can also multiply. Phytoplasmas can cause serious and devastating problems for crop...
Humana Press, 2011. - 328 p. - Methods in Molecular Biology, Vol. 682. - Recent advances in organic chemistry, fluorescent microscopy, and materials science have created an entirely new range of techniques and probes for imaging DNA damage in molecular and cellular biology. In DNA Damage Detection In Situ, Ex Vivo, and In Vivo: Methods and Protocols, expert researchers explore...
Humana Press, 2006. — 389 p. Series: Methods in Molecular Biology, № 335 Although fluorescent probes and assays, which use energy transfer (ET) for monitoring DNA reactions, have multiplied in recent years, until now there have been no manuals summarizing the many different protocols and probe designs. Fluorescent Energy Transfer Nucleic Acid Probes: Designs and Protocols is...
Humana Press, 2002. - 279 p. - Methods in Molecular Biology, Vol. 203. - Detection and analysis of DNA damage is critically important in the study of the widest variety of biological phenomena, ranging from apoptosis and aging to heart diseases and anticancer drug development. In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts...
Humana Press, 2017. — 213 p. — (Methods in Molecular Biology 1644). — ISBN10: 1493971859, ISBN13: 978-1493971855. This volume presents a comprehensive collection of quick assays for the detection of nuclear and mitochondrial DNA damage and its effects in live and fixed cells and tissues, and in bacterial genomes. Although, such rapid techniques are in demand in the "research...
Humana, 2025. — 286 p. — ISBN-13 : 978-1071644133 This volume provides detailed, up-to-date methods used in basic and applied research on T-cell activation. Chapters explore the fundamentals in T-cell biology a board range cutting-edge techniques from initial Ca2+ live-cell imaging to downstream effector functions in autoimmune disease. Written in the format of the highly...
Humana Press, 2018. — 223 p. — (Methods in Molecular Biology 1724). This volume provides established approaches for identifying, characterizing, and manipulating circRNAs in vitro, in vivo, and in silico. Chapters highlight the breakthroughs and the challenges in this new field of research. Written in the highly successful Methods in Molecular Biology series format, chapters...
Humana Press, 2014. — 366 p. Series: Methods in Molecular Biology, № 1090 In this volume, expert researchers in the field provide detailed experimental procedures for each step of the flux quantification workflow. Steady state and dynamic modeling are considered, as well as recent developments for the reconstruction of metabolic networks and for a predictive modeling. Written in...
Humana Press, 2011. — 434 p. — (Methods in Molecular Biology 675). International biobank collaborations allow for studies with large number of subjects where generalizability of findings across populations can be investigated, which means establishing quality criteria concerning the nature of the sample, conditions of sample storage, and the adequacy of available information is...
Humana Press, 2018. — 396 p. This volume presents detailed laboratory procedures in an easy to follow format that can be carried out with success by investigators lacking previous exposure to a specific research method. Chapter guide readers through the application of molecular approaches to disease gene identification and overviews, and case studies are also presented. Written...
Humana Press, 2021 — 180p. — (Methods in Molecular Biology 2354). — ISBN 978-1-0716-1608-6. This volume offers a comprehensive collection of experimental approaches to study Solanum tuberosum (potato). Chapters are divided into five sections covering a general overview of potato, the research fields of molecular biology, omics approaches, and bioinformatics, and finally, the...
Springer Science+Business Media LLC, 2017. — 283 p. — (Methods in Molecular Biology 1620) — ISBN10: 1493970593. This volume details PCR technique with focus on its application specificities to the biotechnology and bioengineering field. Chapters are broken into five sections covering sgeneral PCR protocols, different applied examples to molecular and synthetic biotechnology,...
2nd edition. — Humana Press, 2023. — 255 p. — (Methods in Molecular Biology 2967). — ISBN 978-1-0716-3357-1. This second volume focuses on PCR methods and PCR application specificities to the biotechnology and bioengineering field. New and updated chapters detail real-time PCR protocols, synthetic biology applications, pathogen detection, microfluidics, digital, multiplex...
Humana Press, 2014. — 395 p. Series: Methods in Molecular Biology, № 1147 The discovery that most of the chronic infections in humans, including the oral, lung, vaginal and foreign body-associated infections, are biofilm-based, has prompted the need to design new and properly focused preventive and therapeutic strategies for these diseases. Microbial Biofilms: Methods and...
Springer, 2021. — 352 p. — (Methods in Molecular Biology 2353). — ISBN 978-1-0716-1604-8. This volume explores current technologies used to investigate the formation, insertion, and function of metalloclusters associated with proteins. Chapters describe relevant topics about Fe-S cluster metabolism are explored through genetic, biochemical, spectroscopic methods. Written in the...
Humana Press, 2012. — 419 p. — (Methods in Molecular Biology 805). Display technologies have become a very powerful way of generating therapeutic lead molecules and specific reagents for increasing our understanding of biology; however, despite being first described shortly after phage display, the use of ribosome display and related methods have been much less widespread....
2 nd ed. — Humana Press, 2004. — 500 p. — e-ISBN: 1-59259-809-9 Series: Methods in Molecular Biology, № 278 Screening and Optimizing Protein Production in E. coli Isotopic Labeling of Recombinant Proteins From the Methylotrophic Yeast Pichia pastoris Perdeuteration/Site-Specific Protonation Approaches for High-Molecular-Weight Proteins Segmental Isotopic Labeling for Structural...
Humana Press, 2012. - 326 p. Series: Methods in Molecular Biology, №803 The multidisciplinary science of chemical proteomics studies how small molecules of synthetic or natural origin bind to proteins and modulate their function. In Chemical Proteomics: Methods and Protocols, expert researchers in the field provide key techniques to investigate chemical proteomics focusing on...
Humana Press, 2019. - 347 p. - Volume 1949 of Methods in Molecular Biology. This detailed book provides technical approaches to tackle a variety of questions related to intracellular lipid transport in order to improve our understanding at different scales of how lipids are accurately displaced between organelles, across long distances or at membrane contact sites, or within...
2nd ed. — Humana Press, 2024. — 330 p. This fully updated volume explores different classes of lipid transporters, such as lipid transfer proteins, flippases, and scramblases, and significant advances in solving their biological functions. The first half of the book features methodologies to measure the movement of lipids between or in organelle membranes inside eukaryotic...
Humana Press, 2024. — 233 p. — (Methods in Molecular Biology 2730). — ISBN 978-1-0716-3502-5. This volume provides methods to study ice-binding proteins (IBPs), and applications involving these proteins. Chapters are divided into three parts describing identifying, isolating, and purifying ice-binding proteins, characterize both IBPs and ice-nucleating proteins (INPs), and...
Humana Press, 2023. — 230 p. — (Methods in Molecular Biology 2667). — ISBN 978-1-0716-3198-0. This volume aims to help new investigators to the field by providing them with the protocols needed to set up clinically relevant animal models of fungal infection, as well as methods for in vivo immunology.Written in the format of the highly successful Methods in Molecular Biology...
Humana Press, 2017. — 289 p. — (Methods in Molecular Biology 1522). This second edition volume expands on the previous edition by discussing classic techniques, as well as new protocols that focus on the preparation of liposomes, lipid characterization, particle size and charge analysis, drug encapsulation, surface modification, stimuli response, and cellular interaction and...
3rd edition. — Humana Press, 2023. — 301 p. — (Methods in Molecular Biology 2622). — ISBN 978-1-0716-2953-6. This third edition provides new and updated chapters detailing preparation of liposomes, physicochemical characterization of liposomes, lipid analysis, drug encapsulation, surface modification, stimuli response as well as cellular interaction, and biodistribution. Also...
Humana Press, 2009. — 302 p. Series: Methods in Molecular Biology, № 529 DNA microarray technology has revolutionized research in the past decade. Initially an application for mRNA expression studies, the technology now has spread to other applications such as comparative genomic hybridization, SNP and mutation analysis. In this volume experts explore these now commonly used...
New York: Humana Press, 2012. — 492 p. — (Methods in Molecular Biology 863). — ISBN: 978-1-61779-611-1, 978-1-61779-612-8. Includes epigenetic alterations that can be use in clinical practice Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts The epigenetic regulation plays an important role in normal...
Humana Press, 1994. - 310 p. ISBN: 0896032736 Series: Methods in Molecular Biology, №35 Procedures to Improve Difficult Couplings Methods for Removing the Fmoc Group Solvents for Solid-Phase Peptide Synthesis HF Cleavage and Deprotection Procedures for Peptides Synthesized Using a Boc/Bzl Strategy Acid Cleavage/Deprotection in Fmoc/tBu-Solid Phase Peptide Synthesis...
Humana Press, 2012. — 497p. — (Methods in Molecular Biology 847). — ISBN: 978-1-61779-557-2 In 2010 the global area of transgenic crops reached 148 million hectares, an 87-fold increase since 1996, making it the most rapidly adopted technology in the history of modern agriculture. In Transgenic Plants: Methods and Protocols, Second Edition expert researchers in the field...
Humana Press, 2016 — 387p. — (Methods in Molecular Biology 1398) — ISBN: 978-1-4939-3356-3 (eBook), 978-1-4939-3354-9 (Hardcover). This volume describes different up-to-date methodological approaches, ranging from physiological assays to imaging and molecular techniques, to study a wide variety of plant responses to environmental cues. Environmental Responses in Plants: Methods...
2nd edition. — Humana Press, 2022 — 347 p. — (Methods in Molecular Biology 2494). — ISBN 978-1-0716-2296-4. This second edition describes up-to-date methodological approaches, ranging from physiological assays to imaging and molecular techniques, to study a wide variety of plant responses to environmental cues. Chapters are divided into four sections detailing protocols to...
Springer, 2021. — 467 p. — (Methods in Molecular Biology). — ISBN 978-1-0716-0198-3. With the advent of so-called “next generation” sequencing technologies, the study of genetic variation in populations gained a new dimension, turning population genetics into population genomics. While a scaling-up in data set size accompanied this shift, population genomics is more than just...
Springer, 2021. — 467 p. — (Methods in Molecular Biology). — ISBN 978-1-0716-0198-3. With the advent of so-called “next generation” sequencing technologies, the study of genetic variation in populations gained a new dimension, turning population genetics into population genomics. While a scaling-up in data set size accompanied this shift, population genomics is more than just...
Springer, 2019. — 259 p. — (Methods in Molecular Biology 1906). — ISBN: 1493989634. This detailed book provides a set of protocols necessary for the development of a variety of microchip-based electrophoretic assays. It compiles a range of such electrophoretic methods by leading researchers in the field, covering subjects such as microfluidic device fabrication, on-chip sample...
Springer, 2018. — 194 p. — (Methods and Protocols 1895). — ISBN: 1493989219. This detailed volume explores the methods used for most of the recent approaches to suicide gene therapy of cancer, which exploits promoters that are specific to cancer cells, thereby ensuring (or greatly increasing the likelihood) that the therapeutic gene is expressed only in cancer cells. The book...
Humana, 2024. — 243 p. This volume will serve as a guide for students in the field of neurobiology, and be a bridge between basic science researchers, doctors, and surgeons in clinical practice. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents,...
New York: Humana, 2021. — 319 p. This volume explores scientific methodologies currently employed to integrate observational developmental biology, tissue explant and cell-based approaches and genetic/molecular technologies to develop a holistic understanding of craniofacial development. Chapters guide readers through the use of disparate models to study formation of the head...
Humana Press, 2002. — 267 р. — (Methods in Molecular Biology, № 189). — ISBN: 978-1-59259-281-4. Small GTPase binding proteins (GTPases) are an ancient group of proteins that play key roles in almost every aspect of cell biology, from cell proliferation to nuclear transport. In GTPase Protocols: The Ras Superfamily, Edward J. Manser and Thomas Leung have collected the key...
Humana Press, 2014 — 284p. — (Methods in Molecular Biology 1123) — ISBN: 978-1-62703-968-0 (eBook), 978-1-62703-967-3 (Hardcover).
Homing Endonucleases: Methods and Protocols aims at providing molecular biologists with a comprehensive resource to identify and characterize homing endonucleases from genomic sequence, to deduce the biological basis of binding and cleavage...
3rd edition. — Humana Press, 2022. — 541 p. — (Methods in Molecular Biology 2443). This new edition focuses on applied bioinformatics with specific applications to crops, model and diverse plant species. The scope extends from the genome to the phenome and includes aspects of data management, analysis, visualization, and integration. The methods and approaches found within...
3rd edition. — Humana Press, 2022. — 1173 p. — (Methods in Molecular Biology 2443). — ISBN 978-1-0716-2067-0. This new edition focuses on applied bioinformatics with specific applications to crops, model and diverse plant species. The scope extends from the genome to the phenome and includes aspects of data management, analysis, visualization, and integration. The methods and...
New York: Humana Press, 2015. — 477 p. This volume is essential for geneticists, molecular biologists, biochemists, and medical doctors interested in the use of mouse models in cancer research. Recent genome studies, together with refined genetic engineering techniques, have greatly increased the value of using mice for research on cancer and other human disorders. The chapters of...
Humana Press, 2014. — 283 p. Series: Methods in Molecular Biology, № 1134 The book describes a number of genetic, biochemical and immunological techniques. These techniques provide an advancing understanding of the pathology, breakdown of the immune system and therapeutic challenges of SLE in both humans and animal models. Written in the highly successful Methods in Molecular...
Humana Press, 2006. — 1598 p.
Series: Methods in Molecular Biology, № 346
Dictyostelium discoideum Protocols presents a comprehensive collection of cutting-edge molecular biological protocols for studying D. discoideum, with emphasis on its utility in the study of fundamental cellular processes including signal transduction, chemotaxis, cell motility, cytokinesis,...
Springer, 2016. - 317 p. - Volume 1345 of Methods in Molecular Biology. This detailed volume focuses on methods for the characterization of aggregation processes that lead to the formation of amyloid fibrils and amyloid oligomers which feature in the etiology of a variety of human disorders collectively known as amyloidoses. The scope of the collection includes techniques for...
Humana Press, 2012. — 564 p. — ISBN: 1617795542, 1617795569 Series: Methods in Molecular Biology, №850 Recent advances in genetics over the last quarter of a century, especially in molecular techniques, have dramatically reduced the cost of determining genetic markers and hence opened up a field of research that is increasingly helping to detect, prevent and/or cure many...
Humana Press, 2012. — 564 p. — ISBN: 1617795542, 1617795569. Series: Methods in Molecular Biology, № 850 Recent advances in genetics over the last quarter of a century, especially in molecular techniques, have dramatically reduced the cost of determining genetic markers and hence opened up a field of research that is increasingly helping to detect, prevent and/or cure many...
Humana Press, 2012. - 296 p. ISBN: 1627030107 Genomic imprinting, the process by which the non-equivalence of the paternal and maternal genomes is established, has been fascinating us for over three decades and has provided many emerging scientists with the chance to hit their stride in a frontier posing many unexpected questions and even more surprising answers. In Genomic...
Humana Press, 2020. — 808 p. — (Methods in Molecular Biology 2058). — ISBN: 978-1-4939-9793-0. This book aims to provide a guide for virologists, translational researchers, and clinicians in the field of cancer research by providing reference protocols and methodologies from vector development through clinical translation. Written in the highly successful Methods in Molecular...
New York: Humana Press, 2014. — 812 p. — (Methods in Molecular Biology 1076). Reflecting the expanding field’s need for reliable protocols, this volume offers techniques from a worldwide team of experts on this versatile and vital subject. The topics covered fall into four broad categories: steady-state fluorescence spectroscopy, time-resolved fluorescence spectroscopy,...
Humana Press, 2002. — 396 p. Series: Methods in Molecular Biology, №201. Library Synthesis and Quality Control Using a Noncovalent Protection Strategy to Enhance Solid-Phase Synthesis Quality Control of Solid-Phase Synthesis by Mass Spectrometry Preparation of Encoded Combinatorial Libraries for Drug Discovery Simple Tools for Manual Parallel Solid Phase Synthesis Cleavage of...
Humana Press, 2015. — 356 p. — (Methods in Molecular Biology 1320).
This volume includes comprehensive and up-to-date coverage of all nucleic-acid-specific steps used in X-ray crystallography, from macromolecule production to structure determination. Chapters dedicated to RNA preparation and crystallogenesis will be of interest to beginners, while chapters focused on data...
Humana Press, 2022. — 287 p. — (Methods in Molecular Biology 2533). — ISBN 978-1-0716-2500-2. This Open Access volume provides comprehensive reviews and describes the latest techniques to study eukaryotic ribosome biogenesis. For more than 50 years ribosomes are a major research topic. Our knowledge about ribosome biogenesis and function such as transcription, mRNA...
Humana Press, 2022 — 287 p. — (Methods in Molecular Biology 2533). — ISBN 978-1-0716-2500-2. This volume provides comprehensive reviews and describes the latest techniques to study eukaryotic ribosome biogenesis. For more than 50 years ribosomes are a major research topic. Our knowledge about ribosome biogenesis and function such as transcription, mRNA modification, and...
Springer, 2017. — 224 p. — (Mvtthods in Molecular Biology 1663). — ISBN10: 1493972642. This volume presents current advanced technologies and methods used in super-resolution microscopy. The chapters in this book cover a wide range of topics such as introducing super-resolution microscopy into a core facility; two-photon STED microscopy for nanoscale imaging of neural...
Humana Press, 2009. — 385 p. Series: Methods in Molecular Biology, № 559 The ability to detect and quantify apoptosis, to understand its biochemistry, and to identify its regulatory genes and proteins is crucial to biomedical research in the 21st century. In Apoptosis Methods and Protocols, highly experienced researchers in the field of apoptosis describe in step-by-step detail...
Humana Press, 2018. — 258 p. — (Methods in Molecular Biology 1771). — ISBN 978-1-4939-7791-8. This volume provides a comprehensive look at the current fabrication methods, materials, cell patterning techniques, and practical application of live-cell microarrays. The chapters in this book are categorized into six parts: introduction, biointerface engineering and live-cell...
Humana Press, 2018. — 526 p. — (Methods in Molecular Biology 1771). — ISBN 978-1-4939-7792-5. This volume provides a comprehensive look at the current fabrication methods, materials, cell patterning techniques, and practical application of live-cell microarrays. The chapters in this book are categorized into six parts: introduction, biointerface engineering and live-cell...
New York: Humana Press, 2015. — 241 p. — (Methods in Molecular Biology 1291). This volume brings together the skills and protocols of numerous laboratories that are at the heart of investigation into the biology of Tfh cells in both mice and humans. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics,...
2nd Edition. — Springer Science+Business Media LLC, 2017. — 412 p. — (Methods in Molecular Biology 1606) — ISBN: 9781493969890. This second edition provides an update on technology and an accelerated tutorial to assist, students, entrepreneurs, new investigators, and established investigators who want to quickly become versed in, and immersed in, the entire process from...
Humana Press, 2015. — 245 p. Series: Methods in Molecular Biology, № 1242 This volume covers broad aspects of cell expansion in three different cell types: root hairs, pollen tubes, and hypocothyl cells. Chapters focus on the cutting-edge methods to study in detail several complex aspects of cell expansion such as secretion, endocytosis and recycling, cellular signaling and...
New York: Humana, 2021. — 321 p. This volume presents state-of-the art design, analysis and integration approaches for biomedical data including novel statistical models for a comprehensive and powerful synthesis and assessment of scientific evidence. Chapters detail principles of systematic reviews, semi-automated tools for systematic searches, fixed- and random-effects...
Humana Press, 2016 — 281p. — (Methods in Molecular Biology 1401) — ISBN: 978-1-4939-3375-4 (eBook), 978-1-4939-3373-0 (Hardcover).
This volume presents an overview of nonribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) structure and function. It then continues with methods for the analysis of these pathways including conventional enzymological assays,...
Humana Press, 1999. — 206 p. Septic shock remains a serious medical condition with high mortality. Despite many advances in intensive care medicine and antibiotic development, this has not changed appreciably in the last 20 years. Frustratingly, over the same period of time, enormous advances have been made in understanding the underlying pathogenic mechanisms of this...
Humana Press, 2008. — 452 p. Series: Methods in Molecular Biology, № 415 Immunologists today are interested in all of the diverse cell-types involved in host defense and have a deeper appreciation of the importance of innate immune mechanisms as a first line of protection against pathogens. This volume thus discusses the isolation and functional characterization of cells involved...
2nd edition. — Humana Press, 2022. — 424 p. — (Methods in Molecular Biology 2418). — ISBN 978-1-0716-1919-3. This fully updated collection includes chapters on a wide array of techniques that are vital for advancing our understanding of the physiological and pathological effects of estrogen, mediated by estrogen receptor α (ERα) and estrogen receptor β (ERβ) as well as the...
Humana Press, 2007. — 494 p. — (Methods in Molecular Biology 380). The book is a comprehensive guide to the techniques currently used for culturing and characterising the cell types responsible for imposing self-tolerance and the experimental models employed to study their function both in vitro and in vivo. Provides up-to-date reviews of the literature on essential topics in...
Humana Press, 2023. — 272 p. — (Methods in Molecular Biology 2700). — ISBN13 9781071633656. — ISBN10 1071633651. This volume provides methods on the role of TLRs in immune responses and on the various approaches targeting different TLRs in cancer, autoimmunity and in vaccine development. Written in the highly successful Methods in Molecular Biology series format, chapters...
Humana Press, 2012. — 354 p. — (Methods in Molecular Biology 822). The rapid pace of microRNA (miRNA) research continues to drive the advances of techniques for miRNA expression profiling, and innovative technologies that are more sensitive, specific, quantitative, and that are compatible with a wide range of biospecimens have been developed during the past few years. In this...
Humana Press, 2017. — 550 p. — (Methods in Molecular Biology 1646). — ISBN: 978-1-4939-7194-7. This collection thoroughly explores the dynamic and ever-developing field of hemostasis and thrombosis diagnostics and research. After an introductory section covering the basics and preanalytical issues, the book continues with in-depth sections that explore how to get the best...
Humana Press, 2022. — 243 p. — (Methods in Molecular Biology 2504). This detailed book provides an exhaustive picture of current methods to detect, isolate, and analyze extracellular vesicles (EVs) from diverse sources, now seen as potential disease biomarkers as well as tools for the development of new therapies. Beginning with a section on detection and isolation of EVs, the...
New York: Humana Press, 2019. — 298 p. This book provides protocols to quantify metabolism, to identify metabolic crosstalk, and to setup and develop tools and models to gain insight into metabolic signaling using experimental and computational approaches. Chapters detail protocols to quantify metabolism, identify metabolic crosstalk, and develop tools and models to gain a...
Humana Press, 2016 — 298p. — (Methods in Molecular Biology 1402) — ISBN: 978-1-4939-3378-5 (eBook), 978-1-4939-3376-1 (Hardcover). This volume assembles a broad spectrum of methods used in long non-coding RNAs (lncRNA) research, ranging from computational annotation of lncRNA genes to molecular and cellular analyses of the function of individual lncRNA. Long Non-Coding RNAs:...
Humana, 2025. — 280 p. — ISBN-13 : 978-1071644577 This volume covers a range of fundamental and advanced techniques and methods used by researchers to study the control of yellow fever. The chapters in this book cover a broad range of topics including epidemiological studies that track the spread of disease and sophisticated laboratory techniques that investigate the disease’s...
Humana Press, 2017. — 279 p. — ISBN10: 1493972243, 13 978-1493972241. Series: Methods in Molecular Biology, № 1653 This volume presents a comprehensive overview of contemporary methods to analyze photorespiration in higher plants. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the...
Humana Press, 2017. — 297 p. — (Methods in Molecular Biology 1581). — ISBN 978-1-4939-6867-1. This volume provides readers with methods and protocols for understanding the development of recombinant viruses and their use as vaccines platforms. Recombinant Virus Vaccines: Methods and Protocols details the use of recombinant vaccines that are employed to either produce immunogens...
Garland Science, 2021. — 285 p. How can we understand the complexity of genes, RNAs, and proteins and the associated regulatory networks? One approach is to look for recurring types of dynamical behavior. Mathematical models prove to be useful, especially models coming from theories of biochemical reactions such as ordinary differential equation models. Clever, careful...
Humana Press, 2016 — 180p. — (Methods in Molecular Biology 1405) — ISBN: 978-1-4939-3393-8 (eBook), 978-1-4939-3391-4 (Hardcover). This volume describes up-to-date techniques for improved production of secondary metabolites of economic interest using field and laboratory methods. Biotechnology of Plant Secondary Metabolism: Methods and Protocols explores different secondary...
Humana Press, 2014 — 208p. — (Methods in Molecular Biology 1106) — ISBN: 978-1-62703-736-5 (eBook), 978-1-62703-735-8 (Hardcover). Due to the highly collaborative nature of investigators working in the field, we have rapidly advanced our understanding of Staphylococcus epidermidis and other staphylococci in the last two decades. The chapters in Staphylococcus Epidermidis:...
New York: Humana Press, 2015. — 226 p. This volume provides a collection of protocols for studying and manipulating VEGF signaling pathways in vitro and in vivo, and in particular, aims to present a range of both firmly established and newly emerging technologies, including those that are amenable to aiding in drug discovery or pre-clinical investigations. Each of the three...
Humana, 2007. — 244 p. Peptide Characterization and Application Protocols is dedicated entirely to the characterization of peptides and their applications for the study of biochemical systems and the contributing authors are all leaders in the field of peptide research. Part I, Characterization, presents the most recent advances in select analytical techniques, including...
ITexLi, 2018. — 57 p. — ISBN 1789847281 9781789847284 183881597X 9781838815974. In this book are presented both methods for the determination of homocysteine and its involvement in human pathology. The first description of homocysteine (Hcy), a non-proteinogenic amino acid, was introduced in a case study more than 70 years ago. In recent years, numerous studies have shown a...
Humana Press, 2023. — 379 p. — (Methods in Molecular Biology 2627). — ISBN 978-1-0716-2973-4. This detailed volume provides state-of-the-art methodologies and reviews of important topics in the field of homology modeling. From homology modeling in the twilight zone and improving accuracy through sequence space analysis to approaches to construct multi-protein complex models,...
Humana Press, 2014. — 800 p. Series: Methods in Molecular Biology, № 1149 In this volume, expert researchers in the field detail many of the methods which are now commonly used to study this fascinating microorganism. Chapters include microbiological methods to high-throughput molecular techniques that have been developed over the last decade. Written in the highly successful...
New York: Humana Press, 2023. — 228 p. This volume explores techniques used to study and understand the latest research on pyroptosis. The chapters in this book cover topics such as experimental methods to induce pyroptosis via inflammasomes; approaches to identifying inflammasome activation in humans; ways to induce and detect pore formation; and techniques to evaluate...
Humana Press, 2014. — 267 p. Series: Methods in Molecular Biology, № 1145 The recent advent of molecular technologies has changed the way plant breeders identify and select their germplasm as genetic variation can now be assessed at the DNA level. This volume presents detailed guidelines and tutorials that suit different needs and capacity from small laboratories analyzing...
Springer, 2021. — 337 p. — (Methods in Molecular Biology 2226). — ISBN 978-1-0716-1019-0. This volume aims at providing a selection of the most effective and widely employed protocols used in the Ewing sarcoma field. Chapters are divided into five sections detailing the molecular biology techniques used to analyze Ewing sarcoma including tissue preservation and methods used in...
Humana Press, 2018. - 412 p. - Volume 1716 of Methods in Molecular Biology. This volume looks at the latest methodologies used to study cellular metabolism with in silico approaches. The chapters in this book are divided into 3 parts: part I discusses tools and methods used for metabolic reconstructions and basic constraint-based metabolic modeling (CBMM); Part II explores...
Humana Press, 2014 — 299p. — (Methods in Molecular Biology 1122) — ISBN: 978-1-62703-794-5 (eBook), 978-1-62703-793-8 (Hardcover). Metalloproteins are involved in many key biological processes such as gas transport and metabolism, photosynthesis, cell respiration, the Krebs cycle and many other vital redox reactions. Metalloproteins: Methods and Protocols addresses multiple...
3rd Edition. — Springer, 2020. — 302 p. — (Methods in Molecular Biology 2135). — ISBN: 978-1-0716-0462-5. This third edition provides revised and expanded protocols of consolidated approaches as well as new trends in the field. Chapters guide readers through new approaches to optimize Quantum Dots’ (QD) properties, to evaluate their quantum yields, important features about...
2nd edition. — Humana Press, 2014. — 258 p. — (Methods in Molecular Biology 1199). — ISBN: 978-1-4939-1280-3, 978-1-4939-1279-7. In Quantum Dots: Applications in Biology, Second Edition, expert researchers in the field detail consolidated approaches as well as new trends in the field. Organized into five parts, the first part comprises an introduction on Quantum Dots (QDs) as...
Humana Press, 2017. — 279 p. — (Methods in Molecular Biology 1633). — ISBN 978-1-4939-7140-4. This volume describes up-to-date techniques used in understanding the molecular biology of acute myeloid leukemia (AML) and addressing advances in diagnosis, classification, prognostication, and therapeutic strategies to potentially impact overall patient survival. The chapters in this...
2nd Edition. — Springer, 2021. — 255 p. — (Methods in Molecular Biology 2220). — ISBN: 978-1-0716-0982-8. This updated book explores a wide repertoire of tools and approaches that have been created, modified, and applied to the study of L. monocytogenes, forming the basis of our understanding of the bacterium today. Many of these key experimental techniques are gathered...
New York: Humana Press, 2010. — 321 p. The last few years have witnessed the creation of new generations of sequence reading compounds, which have incredible potential for targeting specific DNA sequences. In Drug-DNA Interaction Protocols, Second Edition, expert researchers explore these compounds, revealing how a deeper understanding of the structure of DNA, along with an...
Humana Press, 2021. — 359 p. — ISBN 978-1-0716-1668-0. This detailed book serves as a systematic examination of the analytical methods to study the transcription factor NF-κB in physiology and disease. It provides an up-to-date guidebook to navigate both conventional and highly specialized methods to detect and analyze the different signaling pathways of NF-κB activation and...
Humana Press, 2021. — 359 p. — ISBN 978-1-0716-1668-0. This detailed book serves as a systematic examination of the analytical methods to study the transcription factor NF-κB in physiology and disease. It provides an up-to-date guidebook to navigate both conventional and highly specialized methods to detect and analyze the different signaling pathways of NF-κB activation and...
Humana Press, 2019. - 399 p. - Volume 1860 of Methods in Molecular Biology. This book details multiple ways that soluble N-ethylmaleimide-sensitive factor attachment protein receptors( SNAREs) and their function are examined in the laboratory. The methods described in each chapter are described in detail so that novice as well as experienced researchers can explore the mechanisms...
3rd ed. — Humana Press, 2024. — 307 p. This updated volume provides a concise set of protocols on the conceptual principles and practical considerations pertinent to research in mitochondrial function. Driven by the impact of drug- and environmental chemical-induced disturbances on mitochondrial function, hereditary deficiencies, and the age-related decline in bioenergetic...
Humana Press, 2013. — 385 p. Series: Methods in Molecular Biology, № 1027 The lipid-rich and otherwise challenging nature of many key tissues complicates many aspects of current research, and applications of the unique nature of lipoproteins and their biological effects has engendered unique and vital methodologies. In this volume experts in the field present a compendium of...
Humana Press, 2023. — 297 p. — (Methods in Molecular Biology 2682). — ISBN13 9781071632826. — ISBN10 1071632825. This volume summarizes key methods and advancements in the field of henipavirus molecular biology. Chapters guide readers through protocols focusing on emerging bat-borne paramyxoviruses as well as pathogenicity caused by Nipah and Hendra viruses. Protocols are also...
6th edition. — Wiley-Blackwell, 2010. — 796 p. This is the sixth edition of the leading text in the basic methodology of cell culture, worldwide. Rigorously revised, it features updates on specialized techniques in stem cell research and tissue engineering; updates on molecular hybridization, somatic cell fusion, hybridomas, and DNA transfer; new sections on vitrification and...
New York: Humana Press, 2023. — 377 p. This volume provides a collection of protocols from researchers in the statistical genomics field. Chapters focus on integrating genomics with other “omics” data, such as transcriptomics, epigenomics, proteomics, metabolomics, and metagenomics. Written in the highly successful Methods in Molecular Biology series format, chapters include...
2nd edition. — Humana Press, 2023. — 501 p. — (Methods in Molecular Biology 2644). — ISBN 978-1-0716-3051-8. This updated edition explores assessing cell viability as a measure for cell fitness under conditions of physiological and patho-physiological stress as well as challenging conditions to cellular and tissue homeostasis, and accounts for the ongoing 2D-to-3D development...
2nd edition. — New York: Humana Press, 2022. — 526 p. This volume serves to aid researchers working in the recombinant protein production field by describing a wide number of protocols and examples. Chapters describe recombinant protein production in different expression systems, prokaryotic and eukaryotic expression systems, purification protocols, characterization of...
Humana Press, 2004. — 501 p.
Series: Methods in Molecular Biology, № 261
Proteins continuously interact with each other to determine cell fate. Consequently, an examination of just when such protein-protein interactions occur and how they are controlled is essential for understanding the molecular mechanism of biological processes, elucidating the molecular basis of diseases,...
2nd Edition. - Humana Press, 2019. - 380 p. - Volume 2024 of Methods in Molecular Biology. This second edition volume expands on the previous edition with new sections describing the characterization of peptides bound to major histocompatibility complexes (MHC) on the surface of the cell. Chapters also cover topics such as using SERPA for antigen identification; antigen content...
Humana Press, 2014. — 384 p. — (Methods in Molecular Biology 1100). The book is composed of 32 individual chapters that describe a variety of protocols to purify and analyze the activity of the individual complement components or pathways. It includes assays that describe detection of complement SNPs, clinical methods to evaluate complement system activation and data...
Humana Press, 2019. - 222 p. - Volume 1951 of Methods in Molecular Biology. This book covers a wide range of state-of-the-art methodologies and detailed protocols currently used to study the actions that lipid-activated nuclear receptors and their co-regulators have in tissues and immune cell types considered classic metabolic “powerhouses”. This includes the liver, adipose...
Humana Press, 2013. — 534 p. Series: Methods in Molecular Biology, № 965 Cell senescence is the process whereby cells permanently lose the possibility to proliferate without undergoing cell death, and occurs in a plethora of distinct model organisms. In this volume, expert researchers in the field detail the methods that are now commonly used to study cell senescence, in model...
Second Edition. — Humana Press, 2013. — 430 p. Series: Methods in Molecular Biology, № 998 Every cell in our bodies contains a great variety and number of permeability pathways for various organic and inorganic ions, water, metabolites, nutrients, and signalling molecules. Maintenance and precise control of gating within these pathways are fundamental principles of life as...
Humana Press, 2023. — 252 p. — (Methods in Molecular Biology 2703). — ISBN 978-1-0716-3388-5. This volume discusses the latest online plant genomics and cytogenetic resources used by plant evolutionary biologists and plant breeders. The chapters in this book are organized into two parts. Part One looks at plant genomic databases, and covers topics such as plant phenomics and...
Humana Press, 2023. — 252 p. — (Methods in Molecular Biology 2703). — ISBN 978-1-0716-3389-2. This volume discusses the latest online plant genomics and cytogenetic resources used by plant evolutionary biologists and plant breeders. The chapters in this book are organized into two parts. Part One looks at plant genomic databases, and covers topics such as plant phenomics and...
Humana Press, 2023. — 327 p. — (Methods in Molecular Biology 2679). — ISBN 978-1-0716-3270-3. This detailed volume explores recent developments in microfluidics technologies for cancer diagnosis and monitoring. The book is divided into two sections that delve into techniques for liquid biopsy for cancer diagnosis and platforms for precision oncology or personalized medicine in...
New York: Humana Press, 2015. — 443 p. With insolubility proving to be one of the most crippling bottlenecks in the protein production and purification process, this volume serves to aid researchers working in the recombinant protein production field by describing a wide number of protocols and examples. Insoluble Proteins: Methods and Protocols includes chapters that describe not...
Humana Press, 2016 — 434p. — (Methods in Molecular Biology 1400) — ISBN: 978-1-4939-3372-3 (eBook), 978-1-4939-3370-9 (Hardcover). This volume covers the latest protocols designed to identify and characterize TEs in genomes, ancient or recently inserted. Additionally, this book includes a series of protocols designed to understand how host genomes act to regulate the activity...
New York: Humana Press, 2023. — 469 p. This fully updated volume describes methods and protocols for a number of drugs and toxins in a stepwise manner. Exploring the versatility and flexibility of mass spectrometry, the book covers the advantages of this technology, which typically include elimination of the need for special reagents such as antibodies, increased sensitivity...
Humana Press, 1995. - 412 p. ISBN: B001DA0B6O Series: Methods in Molecular Biology, №44 Agrobacterium Protocols offers beginning and experienced researchers the most comprehensive collection of step-by-step protocols for the genetic manipulation of plants using Agrobacterium. The topics range from the maintenance of bacterial culture collections to aspects of the metabolism and...
Humana Press, 2018. - 492 p. - Volume 1649 of Methods in Molecular Biology. This volume introduces different concepts and methods of detecting RNA in biological material in a variety of model systems. The chapters in this book discuss methods that will answer numerous biological questions that arise in the study of RNAs. Some of the topics covered in this book are single mRNA...
Springer, 2020. — 265 p. — (Methods in Molecular Biology 2112). — ISBN: 978-1-0716-0269-0. This volume looks at the latest techniques used to perform comparative structure analyses, and predict and evaluate protein-ligand interactions. Cutting-edge and comprehensive, Structural Bioinformatics: Methods and Protocols is a practical guide for researchers to learn more about the...
Springer Science+Business Media LLC, 2017. — 268 p. — (Methods in Molecular Biology 1536) — ISBN: 1493966804 Oat is a cereal grain belonging to the genus Avenae which contains more than 20 species occurring at three ploidy levels, diploid, tetraploid, and hexaploid. The primary cultivated oat (Avena sativa L.) is a hexaploid (2n = 6x = 42) consisting of three genomes, AA, CC,...
Humana Press, 2019. - 434 p. - Volume 1923 of Methods in Molecular Biology. This volume provides an overview of the main yeast production platforms currently used and future yeast cell factories for recombinant protein production. Chapters detail approaches of genetic and metabolic engineering, co-factor containing proteins and virus-like particles, glycoproteins, and...
2nd ed. — Humana, 2025. — 303 p. — ISBN 978-1071644850 This second edition provides new and updated chapters on histones for molecular biologists, biochemists and geneticists. Chapters cover protein complexes that modify chromatin either by adding or removing post-translational modifications, or by exchanging histone variants within the nucleosome. Written in the highly...
Humana Press, 2015 — 267p. — (Methods in Molecular Biology 1266) — ISBN: 978-1-4939-2272-7 (eBook), 978-1-4939-2271-0 (Hardcover).
This detailed volume provides in-depth protocols for protein labeling techniques and applications, with an additional focus on general background information on the design and generation of the organic molecules used for the labeling step. Chapters...
Humana Press, 2010. — 263 p. Series: Methods in Molecular Biology, № 161 In the ten years since the publication of the first edition, great advances in fluorescent labeling, optics, and sample preparation have significantly improved the imaging capability of microscopy, allowing for a continual refinement of our understanding of the cytoskeleton as a dynamic synergy of...
Humana Press, 2013. — 285 p. Series: Methods in Molecular Biology, № 1016 Over the last two decades there has been a growing interest in cyclic nucleotide research in plants with an emphasis on the elucidation of the roles of cGMP and cAMP. In this volume, expert researchers in the field detail many approaches to better understand the biological role of this important signaling...
Humana Press, 2017. — 551 p. — (Methods in Molecular Biology 1486). The aim of this volume is to provide a comprehensive overview of optical tweezers setups, both in practical and theoretical terms, to help biophysicists, biochemists, and cell biologists to build and calibrate their own instruments and to perform force measurements on mechanoenzymes both in isolation in vitro and...
2nd edition. — Humana Press, 2022. — 753 p. — (Methods in Molecular Biology 2478). — ISBN 978-1-0716-2228-5. This detailed volume explores a wide variety of techniques involving optical tweezers, a technology that has become increasingly more accessible to a broad range of researchers. Beginning with recent technical advances, the book continues by covering the application of...
Humana Press, 2023. — 278 p. — (Methods in Molecular Biology 2680). — ISBN 978-1-0716-3274-1. This detailed volume examines fine-tuned methodologies using the planarian species, Schmidtea mediterranea. The book features experimental protocols covering topics from in situ hybridization, immunohistochemistry, cell dissociation and flow cytometry, to pipelines for the analysis of...
New York: Humana Press, 2015. — 195 p. The book explores on the most widely studied of the protein classes, known for their central role in several cellular functions and in a variety of human pathologies. Beginning with a section on activation and signaling of Receptor Tyrosine Kinases (RTKs), the book continues with chapters on aberrant expression of RTKs, trafficking and...
New York: Humana Press, 2022. — 173 p. This volume discusses the latest imaging and molecular techniques for studying microcephaly through neural progenitor proliferation, survival, and gene expression. All of the methods covered in this book use the mouse as a model system, and include cellular, metabolic, transcriptomic studies of neural progenitors, MR imaging studies of...
Humana, 2011. — 498 p. With its complex and extensively regulated metabolism, the study of the RNA lifecycle demands tools that allow for the localization of RNAs to be observed either in an in situ setting or, preferably, under in vivo conditions. In RNA Detection and Visualization: Methods and Protocols, the best and brightest investigators provide an up-to-date and in-depth...
Humana Press, 2013. — 330 p. Series: Methods in Molecular Biology, № 982 While many cytokines are known for their inflammatory action, there is a growing interest in the tissue-protective effects of some cytokines. The prototypic tissue-protective cytokine is EPO. Initially described as neuro-protective, it is beneficial in animal models of ischemic and other types of injury....
Humana Press, 2018. - 448 p. - Volume 1688 of Methods in Molecular Biology. This volume covers state-of-the-art applications of solid-state and solution nuclear magnetic resonance( NMR) spectroscopy to study protein structure, dynamics and interactions. Chapters detail various aspects of data acquisition and processing, determination of the structure, multi-timescale dynamics of...
Humana Press, 2018. — 288 p. — (Methods in Molecular Biology 1812). — ISBN 978-1-4939-8584-5. The main aim of this fourth volume is to complement the first three volumes published in 2004 and 2012 by adding advanced methodologies and perspectives. Chapters guide readers through new techniques into the study of platelets and megakaryocytes, including new imaging approaches, new...
Humana. 2020. — 379 p. This second edition provides updated and new chapters to build on and extend the strengths of the first edition. Chapters guide readers through basic biology of basophils, obtaining the cells by purification, culture of stem cells progenitors, peripheral CD34+ stem cell-derived mast cells, basophils from CD34+ progenitors, diagnostic applications, gene...
Humana, 2024. — 563 p. This second edition volume presents new and updated protocols on comprehensive compendium of clinical metabolomics protocols covering LC-MS, GC-MS, CE-MS, and NMR-based clinical metabolomics as well as bioinformatics and study design considerations. Chapters explore the core of several promising initiatives evolving around personalized health care and...
Humana, 2024. — 262 p. This volume explores the latest ways to detect hypoxia in the context of cancer, including techniques to study gene expression changes, protein responses, and cellular adaptations to low-oxygen conditions. This book also covers new protocols to characterize hypoxia in tumors with high spatial resolution and novel therapeutic approaches that consider the...
2nd Edition. — Springer, 2014. — 383 p. Directed Evolution Library Creation: Methods and Protocols, Second Edition presents user-friendly protocols for both proven strategies and cutting-edge approaches for the creation of mutant gene libraries for directed evolution. As well as experimental methods, information on current computational approaches is provided in a user-friendly...
Humana Press, 2004. — 200 p. — (Methods in Molecular Biology 285). — E-ISBN: 1-59259-822-6. Cell Cycle Control and Dysregulation Protocols focuses on emerging methodologies for studying the cell cycle, kinases, and kinase inhibitors. It addresses the issue of gene expression in vivo and in vitro, the analysis of cyclin-dependent kinase inhibitors, protein degradation mediated...
Humana Press, 2016. — 235 p. — ISBN: 1493963651 Series: Methods in Molecular Biology, №1477 This volume focuses on the developmental areas of biopesticides: production, formulation, application and field efficacy. Chapters guide readers through methods and techniques on environmental, mammalian, safety, and registration. Written in the highly successful Methods in Molecular...
2nd edition. — Humana Press, 2016. — 591 p. — (Methods in Molecular Biology 1416). — ISBN: 978-1-4939-3582-6. This volume aims to outline the current status of the Mesenchymal Stem Cells(MSC) field in regenerative medicine and to propose clear and reproducible protocols to better define the identity, function and use of these cells that are today, more than ever, “under the...
Humana Press, 2020. — 337 p. — (Methods in Molecular Biology 2067). — ISBN 978-1-4939-9840-1. This book provides a toolkit of novel research approaches for investigators to study diabetic nephropathy, including critical experimental models from the fly to the fish, cells in culture, and in vivo mammalian approaches. The collection also explores powerful techniques to image the...
Humana Press, 2022. — 506 p. — (Methods in Molecular Biology 2502). — ISBN 978-1-0716-2336-7. This volume covers all methods used to discover the composition, structure, and dynamics of the nuclear pore complex (NPC), as well as the soluble transport factors involved in the transport process. Chapters detail structural biology methods, biophysical, transport factors , and a...
Humana, 2023. — 227 p. This volume discusses the latest approaches used to investigate molecular biology and physiology in the peripheral and central olfactory system. Understanding both normal function and pathobiology provides readers with insight into understanding human olfactory disorders. The chapters in this book cover topics such as techniques for manipulating and...
Humana Press, 2019. — 338 p. — (Methods in Molecular Biology 1873). This detailed book gathers a broad collection of experimental approaches to assist researchers in setting up different methods to investigate protein conformational disorders. Beginning with a section on assays focusing on biophysical approaches to study protein (mis)folding, the volume continues with sections...
Springer Science+Business Media, 2013. — 565 p. — ISBN: 1627034463 Series: Methods in Molecular Biology, №1019 With the detailed genomic information that is now becoming available, we have a plethora of data that allows researchers to address questions in a variety of areas. Genome-wide association studies (GWAS) have become a vital approach to identify candidate regions...
Springer, 2021. — 346 p. — (Methods in Molecular Biology 2215). — ISBN 978-1-0716-0965-1. This volume details the most up-to-date cryo-EM techniques from leading researchers. Chapters are organized into four parts with emphasis on electron cryotomography, single particle analysis, and the crystal based cryo-EM methods of 2D electron crystallography, and MicroED for the study of...
Humana Press, 2013. — 467 p. — (Methods in Molecular Biology 1030). Although antiviral drugs have been successfully developed for some viral diseases, there remains a clear, unmet medical need to develop novel antiviral agents for the control and management of many viruses that currently have no or limited treatment options as well as a need to overcome the limitations...
New York: Humana Press, 2022. — 277 p. This detailed volume provides a comprehensive overview of state-of-the-art metabolomics methods based on mass spectrometry (MS), and their application in food, nutrition, and biomedical research. The chapters assembled herein cover hot topics related to sample preparation, chromatographic and electrophoretic separation, MS-based analysis,...
2nd edition. — Humana Press, 2016. — 297 p. — (Methods in Molecular Biology 1420). — ISBN 978-1-4939-3595-6. This volume presents a series of protocols and methods, some of which are not widely used by researchers/practitioners, and will aid in the execution of different laboratory techniques. Forensic DNA Typing Protocols, Second Edition is arranged into a series of related...
Humana Press, 2013. — 363 p. Series: Methods in Molecular Biology, № 1011 It is now well established that jasmonates, originally identified as the major component of jasmine scent, play a universal role in the plant kingdom and are involved in the regulation of diverse aspects of plant biology, including growth, development, metabolism, and interaction with the environment. In...
Humana Press, 2014. — 547 p. Series: Methods in Molecular Biology, №1097 The existence of noncoding RNA genes (ncRNAs) was proposed simultaneously with protein coding genes in 1961 by Jacob and Monod. Since then, a substantial focus has been on protein coding genes, while the area of ncRNA evolved more slowly and received less attention even though major breakthroughs were...
Humana, 2024. — 283 p. This detailed volume explores techniques for performing calcium signaling in diverse cell types, alongside detailed methods for data analysis. Beginning with chapters on cellular assays to assess calcium signaling, the book continues with sections on imaging methods, methods to assess calcium within primary cilia, the endoplasmic reticulum, and the...
Humana Press, 1994. - 492 p. ISBN: 0896032892 Series: Methods in Molecular Biology, №29 Mitotic Metaphase Chromosome Preparation from Peripheral Blood for High Resolution. Chromosome Preparation from Hematological Malignancies. Meiotic Chromosome Preparation. Preparation of Chromosomes for Scanning Electron Microscopy. Immortalized Cell Lines: Chromosome Preparation and...
New York: Humana, 2022. — 195 p. This volume provides essential and fundamental protocols on manipulation chromosome. Chapters details methods on the preparation of mitotic chromosome, chromosome aberration, micronucleus (MN), comet assay, karyotyping, Fluorescent in situ hybridization (FISH), premature chromosome condensation (PCC), immunohistochemistry (IHC) staining, new...
Humana Press, 2004. — 445 p. Series: Methods in Molecular Biology, № 263 The study of RNA interference, editing, and modification has led to major shifts in our understanding of how genes are expressed and regulated. In RNA Interference, Editing, and Modification: Methods and Protocols, hands-on experimentalists describe in detail the protocols and assays they have developed to...
Humana Press, 2022. — 288 p. — (Methods in Molecular Biology 2380). — ISBN 978-1-0716-1735-9. The volume provides a comprehensive overview of methods that have been developed for the study of Tfh and Tfr biology in mice, and in different human diseases. The chapters detail methods to investigate the function of Tfh cells in vivo and in vitro, including the use of mathematical...
Humana Press, 2022. — 595 p. — (Methods in Molecular Biology 2380). — ISBN 978-1-0716-1736-6. The volume provides a comprehensive overview of methods that have been developed for the study of Tfh and Tfr biology in mice, and in different human diseases. The chapters detail methods to investigate the function of Tfh cells in vivo and in vitro, including the use of mathematical...
Second Edition. — Humana Press, 2001. — 182 p. Series: Methods in Molecular Biology, № 167 Since the publication of the first edition of DNA Sequencing Protocols in 1993, major advances in its technology have made PCR-based semiautomated fluorescent sequencing the norm and enabled the complete sequencing of complex genomes. In DNA Sequencing Protocols, 2nd Edn., Colin Graham...
Humana Press, 1993. — 354 p. ISBN: 0896032361 Series: Methods in Molecular Biology, №19 Expert practitioners combine the traditional basic techniques of membrane isolation and analysis with modern advances in molecular biology, immunology, and clinical sciences to devise analytical techniques that are successful for expert and novice alike. The techniques are used in the...
Humana Press, 1993. — 352 p. ISBN: 0896032507 Series: Methods in Molecular Biology, №27 Crystallization of Membrane Proteins for X-Ray Analysis Determination of Cell-Surface Polarity by Solid-Phase Lactoperoxidase Iodination Biochemical Probes of Topography Antipeptide Antibodies The Production of Monoclonal Antibodies to Membrane Proteins
Humana Press, 2000. — 274 p. Series: Methods in Molecular Biology, № 34 In this volume James Gray and Ulrich Desselberger have assembled a comprehensive collection of established and cutting-edge methods for studying and illuminating the structure, molecular biology, pathogenesis, epidemiology, and prevention in animal models of infection with rotaviruses, an important cause of...
3rd edition. — Humana Press, 2023. — 591 p. — (Methods in Molecular Biology 2628). — ISBN 978-1-0716-2977-2. This third volume provides comprehensive protocols on pre-analytical, analytical, plasma, and serum proteomics. New and updated chapters are divided into nine sections, detailing blood processing and handling strategies, discovery- and targeted-based mass spectrometry,...
Humana Press, 1994. — 366 p.
Series: Methods in Molecular Biology, № 24
DNA sequencing has become increasingly efficient over the years, resulting in an enormous increase in the amount of data gen- ated. In recent years, the focus of sequencing has shifted, from being the endpoint of a project, to being a starting point. This is especially true for such major initiatives as...
Humana Press, 1994. — 424 p.
Series: Methods in Molecular Biology, № 25
DNA sequencing has become increasingly efficient over the years, resulting in an enormous increase in the amount of data gener ated. In recent years, the focus of sequencing has shifted, from being the endpoint of a project, to being a starting point. This is especially true for such major initiatives as...
Humana Press, 1993. — 384 p. ISBN: 0896032485 Series: Methods in Molecular Biology, №23 DNA Sequencing Protocols provides you with the knowledge to become a sequencing expert. An all-star cast of investigators covers virtually all aspects, including developments in cycle sequencing, sequencing PCR products, sequencing lambda and cosmids, multiplex sequencing, direct blotting...
ITexLi, 2020. — 433 p. — ISBN 1839680407 9781839680403 1839680415 9781839680410. This book covers a wide range of modern methods involved in experimental biology. This book provides an overview of practical and theoretical aspects of fluorescence methods and their successful application in the investigation of static and dynamic processes in living cells and microorganisms. It...
Springer, 2023. — 800 p. Language, as a system we use to communicate, represents the brain’s biologically perfected machinery for converting thoughts (ideas, concepts, and reflections of both the outside world and our inner feelings) into words and sentences. Crucially, this process occurs in real time. How hundreds of billions of neurons within the dark of the skull control...
Humana Press, 2010. — 395 p. — (Methods in Molecular Biology 624). — ISBN 978-1-60761-608-5. Early detection of cancer at the cellular level, even before anatomic anomalies are visible, is critical to more efficacious and cost effective diagnosis and therapeutic advances. In Cancer Nanotechnology: Methods and Protocols, an international panel of experts provide the most recent,...
Humana, 2025. — 311 p. — ISBN-13 : 978-1071640869 This detailed volume presents state-of-the-art techniques in ocular regeneration research, pulling together recent animal model systems identifying key genes and regulatory networks that contribute to the regenerative response as well as advances in induced pluripotent stem cell (iPSC) biology. The book opens with a section on...
Humana Press, 2003. — 433 p. As more and more plant genomes are sequenced, plant researchers have been inundated with an avalanche of novel methodologies to identify the function of tens of thousands of plant genes. In Plant Functional Genomics, Erich Grotewold has assembled a team of leading plant scientists to describe in detail the most commonly used methods for...
Humana Press, 2016 — 2nd Ed. — 256p. — (Methods in Molecular Biology 1381) — ISBN: 978-1-4939-3204-7 (eBook), 978-1-4939-3203-0 (Hardcover). This second edition volume brings together the experiences of leading scientists in the discipline of cancer gene profiling. Because cancer genes can be profiled in many different ways, Cancer Gene Profiling: Methods and Protocols, Second...
Humana Press, 2004. - 428 p. Series: Methods in Molecular Biology, №243 Prominent experts from around the world detail the chromatographic and electroseparation techniques they have developed for chiral separations on an analytical scale. Described in step-by-step detail to ensure successful experimental results, the procedures are presented as either general methods or as...
Humana Press, 2022. — 417 p. — (Methods in Molecular Biology 2511). This detailed volume explores the application of multiplex biomarker methods in the critical area of COVID-19 research through state-of-the-art technologies in the fields of genomics, proteomics, transcriptomics, metabolomics, and imaging. The book features a series of protocols from labs across the globe...
Humana Press, 2016. — 315 p. — ISBN: 1493967290. Series: Methods in Molecular Biology, № 1546 This detailed book arrives as there is an increasing need for multiplex biomarker readouts for improved clinical management and to support the development of new drugs by pharmaceutical companies, due to continuous technical developments and new insights into the high complexity of...
Humana Press, 2019. — 334 p. — (Methods in Molecular Biology 1916). — ISBN: 978-1-4939-8994-2 This volume details reviews and protocols on the development and analysis of both cellular and animal-based pre-clinical models in a number of medical areas, including metabolic disorders, longevity, cancer, heart disease and psychiatric disorders. Written in the highly successful Methods...
Humana Press, 1999. — 213 p. — Methods in Molecular Biology, Vol. 127. In Molecular Methods in Developmental Biology: Xenopus and Zebrafish, Matthew Guille assembles a hands-on collection of basic and essential molecular and embryological techniques for studying Xenopus and zebrafish. Easily reproducible and designed to succeed, these detailed methods include cellular techniques,...
Humana Press, 2017. — 277 p. — (Methods in Molecular Biology 1528). This volume provides a comprehensive list of protocols for molecular biologists, biochemists and geneticists. Chapters cover protocols that further the study into protein complexes that modify chromatin either by adding or removing post-translational modifications, or by exchanging histone variants within the...
Humana Press, 2017. — 301 p. — (Methods in Molecular Biology 1540). This volume serves as a reference for the dissemination of advances made in the study of Hepatitis B Virus (HBV). Hepatitis B Virus: Methods and Protocols details protocols and techniques ranging from cell culture studies to in vivo and clinical immunology. The chapters in this book discuss treatments of in vitro...
2nd Ed. — Humana Press, 2016 — 226p. — (Methods in Molecular Biology 1375) — ISBN: 978-1-4939-3173-6 (eBook), 978-1-4939-3172-9 (Hardcover).
This volume covers a large area, from the description of methodologies for data analysis to the real application. Chapters focus on methodologies for preprocessing of microarray data, a survey of miRNA Data analysis, Cloud-based...
Humana Press, 2018. — 311 p. — (Methods in Molecular Biology 1850). — ISBN: 978-1-4939-8730-6 (eBook). About this book This detailed volume explores advances in vector design, DNA delivery, cell cultivation, host cell engineering, and bioprocess optimization within the study of recombinant protein expression in mammalian cells. The majority of the protocols employ either...
Humana Press, 2017. — 210 p. — (Methods in Molecular Biology 1589). This detailed volume focuses on population epigenetics, which is of increasing interest to policy makers searching for explanations for complex epidemiological observations and conceptual models on which to base interventions. With a concentration on DNA methylation, the emphasis is on the most commonly studied...
Humana Press, 2003. — 276 p.
Series: Methods in Molecular Biology. Vol. 117
General Preparation of Material and Staining of Sections (Heather A. Davies)
Negative Staining of Thinly Spread Biological Particulates (J. Robin Harris)
Preparation of Thin-Film Frozen-Hydrated/Vitrified Biological Specimens for Cryoelectron Microscopy (J. Robin Harris and Marc Adrian)
The...
Humana Press, 2013. — 279 p. Series: Methods in Molecular Biology, № 981 Thousands of proteins have been identified to be acetylated. Immense research power has been dedicated to experiments to solve the biological implications of each and every protein acetylation. Two particular sites of protein acetylation have been described intensively: the N-terminal methionine residue of...
New York: Humana Press, 2019. — 201 p. This volume explores the scope of the cellular redox analysis and the importance of not being limited by frequently changing and evolving technology. The chapters in this book cover a wide range of topics such as redox components in animal and plant cells and the role of reactive oxygen species, reactive nitrogen species, and hydrogen...
3rd edition. — New York: Humana, 2020. — 221 p. This detailed new edition delves into recently developed protocols for those working on cell nucleus research, including some of the less well-explored areas of study. Like the previous editions, this book features contributions from top experts in their respective fields. Written for the highly successful Methods in Molecular...
2nd Edition. — Humana Press, 2015. — 236 p. — ISBN: 9781493916795 Series: Methods in Molecular Biology, № 1228 This volume presents detailed, recently-developed protocols ranging from isolation of nuclei to purification of chromatin regions containing single genes, with a particular focus on some less well-explored aspects of the nucleus. The methods described include new...
Humana Press, 2008. — 323 p. — (Methods in Molecular Biology 463). — ISBN: 978-1-59745-406-3 (eBook), 978-1-61737-863-8 (Softcover), 978-1-58829-977-2 (Hardcover). Although our understanding of the structure and activities of the cell nucleus and of the nanomachines which it contains is increasing rapidly, much remains to be learned. The application and continuing development...
Humana Press, 2008. — 444 p. — (Methods in Molecular Biology 464). — ISBN: 978-1-60327-461-6 (eBook), 978-1-61737-898-0 (Softcover), 978-1-60327-460-9 (Hardcover). Although our understanding of the structure and activities of the cell nucleus and of the nanomachines which it contains is increasing rapidly, much remains to be learned. The application and continuing development...
Humana Press, 2017. - 431 p. Volume 1548 of Methods in Molecular Biology. This volume provides basic and advanced laboratory protocols for the non-specialist and the experienced researcher. Chapters are divided into three parts covering synthesis and characterization of AMPs, studying the interaction of AMPs with model systems or bacteria, and assaying selected biological...
Springer, 2019. — 229 p. — (Methods in Molecular Biology 2245). — ISBN 978-1-0716-1118-0. This detailed book collects a variety of methods from investigators at the forefront of chondrocyte biology and pathology research that they have tailored for their research projects in order to tackle the unique properties and challenges of the chondrocyte and those of its tissue matrix....
New Jersey: John Wiley & Sons, Inc., 2016. — 412 p. As a modern composite scientific discipline, Cell Biology has expanded and moved forward rapidly in recent years. Cell Biologists now require a wide range of techniques, including those of analytical biochemistry and microscopy in all its diverse forms. These are often used alongside the techniques of molecular biology and...
Humana Press, 2012. — 289 p. — (Methods in Molecular Biology 801). — ISBN 978-1-61779-351-6. Through all of the recent progress provided by high throughput DNA sequencing technologies, it has become clearer and clearer that the study of proteins and protein organelles will be the key to unlocking our ability to manipulate cells and intervene in human disease. In Protein...
Humana Press, 1996. — 512 p. — (Methods in Molecular Biology 58). An essential core collection of the latest molecular and genetic techniques for cloning, subcloning, sequencing, PCR, protein expression, and much more. Each protocol represents a time-tested, step-by-step recipe that creates an understanding of the procedure, easily reproducible results, and confidence that the...
Humana Press, 1994. - 388 p. ISBN: 0896032582 Series: Methods in Molecular Biology, №31 Basic Recombinant DNA Techniques:Transformation of Bacteria by Electroporation. Direct Cloning of lgt11 cDNA Inserts into a Plasmid Vector. PCR Cloning Using T- Vectors. Thermal Cycle Dideoxy DNA Sequencing. Ordered Deletions Using Exonuclease III. In Vitro Mutagenesis: Site Directed...
Humana Press, 2017. — 258 p. — (Methods in Molecular Biology 1630). — ISBN: 978-1-4939-7128-2. This volume details protocols that can be used for generation of knockout animals. Chapters guide the reader through basic protocols for three genome editing technologies, target design tools, and specific protocols for each animal. Written in the highly successful Methods in...
2nd edition. — Humana Press, 2023. — 396 p. — (Methods in Molecular Biology 2637). — ISBN 978-1-0716-3015-0. This second edition provides new and updated protocols that can be used for generation of knockout animals. Chapters guide the reader through basic protocols for three genome editing technologies, target design tools, and specific protocols for each animal. Written in...
Humana Press, 2013. — 271 p. — (Methods in Molecular Biology 1017). The genomes of humans, as well as many other species, are interspersed with hundreds of thousands of tandem repeats of DNA sequences. Those tandem repeats located as codons within open reading frames encode amino acid runs, such as polyglutamine and polyalanine. Tandem repeats have not only been implicated in...
4th Edition. - Humana Press, 2018. - 495 p. - Volume 1678 of Methods in Molecular Biology. This fourth edition volume expands on the previous editions by presenting readers with new and emerging methodologies in cytometry. The chapters in this book cover various topics such as quantifying surface and intracellular protein biomarkers, intracellular cytokine staining, apoptosis...
2nd Edition. - Humana Press, 2004. - 425 p. Series: Methods in Molecular Biology, №263 In addition to providing chapters on the theoretical and practical aspects of flow cytometry, this book contains protocols used specifically for immediate use in the diagnostic and experimental measurements enabled by modern flow cytometric instrumentation. Research protocols cover cell cycle...
Humana Press, 2019. — 491 p. — (Methods in Molecular Biology 1928). This volume details common experimental approaches in studies designed to illuminate various processes used to study cancer metabolism. Chapters are divided into three parts, the first part focuses on protocols commonly utilized in cancer metabolism studies, such as protocols comprising stable isotope labeling...
Humana Press, 2015. — 213 р. Series Methods in Molecular Biology, №1203 Mass Spectroscopy Imaging (MSI) has emerged as an enabling technique to provide insight into the molecular entities within cells, tissues and whole-body samples and to understand inherent complexities within biological metabolomes. In Mass Spectrometry Imaging of Small Molecules: Methods and Protocols,...
Humana, 2016. — 286 p. This volume brings together the most widely used and important protocols currently being employed in researching and understanding bacterial cell wall homeostasis. Chapters in Bacterial Cell Wall Homeostasis cover a variety of subjects, such as: modern microscopy techniques and other biophysical methods used to characterize the subcellular structure of...
Humana Press, 2018. — 437 p. — (Methods in Molecular Biology 1705). This volume looks at modern computational strategies and techniques used in GPCR drug discovery including structure and ligand-based approaches and cheminformatics. The chapters in this book describe how these approaches can be applied to address key drug discovery issues, such as receptor structure modelling,...
New York: Humana Press, 2015. — 341 p. The book provides a unique compendium of currently applied methods to explore plasmodesmata (PD). Opening with overviews of PD structure and their function during development as well as the role of viruses as models for addressing cellular mechanisms in macromolecular trafficking and movement through PD, the book continues with detailed...
Humana Press, 2023. — 563 p. — (Methods in Molecular Biology 2672). This book provides an up-to-date account of the most widespread methods used by specialists in the field of plant cytogenetics and the emerging field of cytogenomics that will likely soon be adapted by more labs. From the classical basic karyological approaches to the most recent genomics-informed and...
Humana Press, 2019. — 770 p. — (Methods in Molecular Biology 1929). This detailed volume explores protocols for studying the many facets of Ca2+-imaging, Ca2+-signaling, and Ca2+-binding along with background information on the principles and application of these techniques. The content of the book delves into 48 chapters including subjects such as data analysis and modern...
Humana Press, 2012. — 657 p. — (Methods in Molecular Biology 816). Studies over the past decade have continued to bring tremendous advances to our understanding of bone biology. New pathways have been discovered and expanded our knowledge of the ways in which genes and gene products affect bone cells and thereby bone mass and bone strength. In this volume expert researchers in...
3rd Edition. - Humana Press, 2004. - 365 p. - In this fully revised edition of an established classic, expert researchers and clinicians describe in step-by-step detail updated techniques for the isolation and growth of the major primary cell types, such as kidney proximal tubule cells, hepatocytes, keratinocytes, and cardiomyocytes. The authors offer readily reproducible new...
3rd edition. — Humana Press, 2024. — 511 p. — (Methods in Molecular Biology 2694). — ISBN 978-1-0716-3376-2. This third edition volume expands on the previous editions with new discussions on the latest techniques and developments in the field. The chapters in this book are organized into four parts, and cover topics such as optical tweezers; single-molecule fluorescence tools;...
Humana Press, 2015. — 331 p. Series: Methods in Molecular Biology, № 1263 This volume seeks to enable the discovery of tools in chemical biology by providing readers with various techniques ranging from initial chemical genetic screening to target identification. To successfully highlight the essential components of the chemical biology tool discovery process, the book is...
Humana Press, 2010. — 443 p. Series: Methods in Molecular Biology, № 655 The beauty and variety of plants has fascinated mankind for thousands of years, and this fascination continues today as plant developmental biologists discover mechanisms that control the establishment of specialized cell types, tissues, and organs from the fertilized egg during a plant’s life. In Plant...
Humana Press, 2006. — 249 p. Series: Methods in Molecular Biology, № 339 Microchip capillary electrophoresis, a new and emerging technology that can rapidly analyze biological compounds as small as or smaller than a single cell, will have a major impact on biological sciences in the next 10 to 20 years. In Microchip Capillary Electrophoresis: Methods and Protocols, leading...
Humana Press, 2014. — 300 p. Series: Methods in Molecular Biology, № 1099 In this volume, expert researchers provides modern protocols for the analysis and manipulation of cereal genomes. Techniques for isolation and analysis of DNA and RNA from both the vegetative tissues and from the more challenging seeds of cereals are described. Tools for the isolation, characterization and...
Humana Press, 2014 — 427p. — (Methods in Molecular Biology 1126) — ISBN: 978-1-62703-980-2 (eBook), 978-1-62703-979-6 (Hardcover).
Providing a guide to classical experimental approaches to decipher splicing mechanisms and experimental strategies that rely on novel multi-disciplinary approaches, Spliceosomal Pre-mRNA Splicing: Methods and Protocols describes the theory of...
Humana Press, 2016 — 2nd Ed. — 292p. — (Methods in Molecular Biology 1397) — ISBN: 978-1-4939-3353-2 (eBook), 978-1-4939-3351-8 (Hardcover). This second edition expands upon the previous volume with additional emphasis on recent innovation in basic renal research and has a more holistic approach on associated disorders such as complications associated with peritoneal dialysis,...
3rd edition. — Humana Press, 2023 — 352 p. — (Methods in Molecular Biology 2664). — ISBN 978-1-0716-3178-2. This third edition provides updated and new chapters on recent innovations in basic renal research. Chapters are divided into three sections covering in vitro, in vivo, and ex vivo models of kidney disease, recent advances in imaging techniques and protocols for...
3rd edition. — Humana Press, 2023 — 352 p. — (Methods in Molecular Biology 2664). — ISBN 978-1-0716-3179-9. This third edition provides updated and new chapters on recent innovations in basic renal research. Chapters are divided into three sections covering in vitro, in vivo, and ex vivo models of kidney disease, recent advances in imaging techniques and protocols for...
Humana Press, 2014. — 261 p. Series: Methods in Molecular Biology, № 1056 In this volume, expert researchers in the field detail many of the techniques used for identification of bioactive compounds from a large selection of fields in plant biology including plant pathogenesis, immune responses, small RNA processing, endomembrane trafficking, lipids, plant hormone signaling and...
Humana Press, 2017. — 234 p. — (Methods in Molecular Biology 1545). This volume covers methods for the analysis of extracellular vesicles (EV) that can be applied to isolated EVs from a wide variety of sources. This includes the use of electron microscopy, tunable resistance pulse sensing, and nanoparticle tracking analysis. The chapters in this book discuss EV cargoes containing...
Humana Press, 2011. — 252 p. — (Methods in Molecular Biology 752). — ISBN 978-1-60327-221-6. Protein misfolding is a key feature of many disorders in humans, given that over twenty proteins are known to misfold and cause disease. In Protein Folding, Misfolding, and Disease: Methods and Protocols, experts in the field present a collection of current methods for studying the...
Humana Press, 2014 — 328p. — (Methods in Molecular Biology 1130) — ISBN: 978-1-62703-989-5 (eBook), 978-1-62703-988-8 (Hardcover). Skeletal Development and Repair: Methods and Protocols is a compilation of a variety of skeletal research protocols utilizing the laboratory mouse as the platform for surgical manipulation and/or transplantation as well as the source of tissues and...
Humana Press, 2014. — 295 p. Series: Methods in Molecular Biology, № 1166 The book details many of the methods and protocols commonly used to study plant cold acclimation and freezing tolerance, breeding, genetics, physiology or molecular biology, or any combination of these specialties. Chapters focus on interdisciplinary approaches, experimental methods, and concepts from...
Humana, 2021. —456 p. This detailed book explores the fundamentals of myofibroblast biology in tissue repair, fibrosis, and tumors as well as providing cutting-edge laboratory methods used to investigate myofibroblast functions in physiological and pathological settings in vitro and in vivo, as written by leading academic scientists. Section I of this volume focuses on...
Humana Press, 2015 — 216p. — (Methods in Molecular Biology 1318) — ISBN: 978-1-4939-2742-5 (eBook), 978-1-4939-2741-8 (Hardcover). This volume is a practical biochemical guide to the Enzyme-Linked Immunosorbent Assay (ELISA), used to detect a target substance in a liquid sample. The ELISA is an important and widely used diagnostic tool in medicine, animal health, botany and...
Humana Press, 2017. — 392 p. — (Methods in Molecular Biology 1628). This vital book collects key methods that have supported advancements in the field of ebolavirus molecular biology given the pressing need for the advancement of techniques for diagnostics, the development of vaccines and antivirals, and for furthering our understanding of ebolavirus biology. After an...
Humana Press, 2016 — 186p. — (Methods in Molecular Biology 1409) — ISBN: 978-1-4939-3515-4 (eBook), 978-1-4939-3513-0 (Hardcover). This volume explores the evolution of bacterial cancer therapy and describes the modern techniques used in therapy today. The chapters in this book cover a broad range of topics such as the development of tumor-targeting Salmonella typhimurium A1-R,...
Humana Press, 2019. — 330 p. — (Methods in Molecular Biology 1866). This book explores the methionine dependence of cancer and its effects on aging, a great story of science that is not widely known. The chapters in this book describe the discovery of methionine dependence of cancer; the molecular basis for the increased methionine demand of cancer cells and tumors; the clinical...
2nd edition. — Humana Press, 2023. — 321 p. — (Methods in Molecular Biology 2684). — ISBN 978-1-0716-3290-1. This fully updated volume explores recently improved avenues to study urothelial carcinomas. Beginning with several novel chapters on molecular characterization and urothelial carcinogenesis, the book continues with sections on cellular and animal models, biomarkers, and...
2nd edition. — Humana Press, 2023. — 321 p. — (Methods in Molecular Biology 2684). — ISBN 978-1-0716-3291-8. This fully updated volume explores recently improved avenues to study urothelial carcinomas. Beginning with several novel chapters on molecular characterization and urothelial carcinogenesis, the book continues with sections on cellular and animal models, biomarkers, and...
Humana Press, 2003. — 400 p. — (Methods in Molecular Biology 209). The generation of genetically modified mice is absolutely crucial to gene function studies today, primarily because mice are genetically similar to man and because gene function studies in mice are in the context of a whole organism, making them particularly useful. In Transgenic Mouse Methods and Protocols,...
Humana Press, 2000. — 341 p.
Series: Methods in Molecular Biology, № 145
The interest of investigators across a broad spectrum of scientific dis- plines has been steadily stimulated by the field of bacterial toxin research, an area that makes use of a large variety of biological, chemical, physicochemical, and medically oriented approaches. Researchers studying bacterial...
2nd Edition. — Springer Science+Business Media LLC, 2017. — 204 p. — ISBN: 9781493969562. Series: Methods in Molecular Biology, № 1600 This second edition volume expands on the previous editions with detailed protocols on (cyano) bacterial and mold fungus toxins, and a specific focus on Gram-negative endotoxins (lipopolysaccharides). The chapters in Microbial Toxins: Methods...
Humana Press, 2013. — 314 p. Series: Methods in Molecular Biology, № 957 Mammalian oocytes occupy a critical nexus in reproduction as they not only contribute half the genetic makeup of the embryo but also provide virtually all of the cytoplasmic building blocks required for sustaining embryogenesis. The journey that transforms a primordial germ cell into a mature oocyte (or...
Humana Press, 2000. — 403 p. — (Methods in Molecular Biology 32). With the molecular mechanisms underlying the various forms of muscular dystrophy now rapidly clarifying, precise diagnosis has become a reality, and even a requirement in clinical practice. In Muscular Dystrophy: Methods and Protocols, Katherine Bushby and Louise Anderson have assembled an outstanding collection of...
Humana Press, 2012. — 556 p. During the 20th century Xenopus has flourished as an accessible experimental model system for studying vertebrate biology. The second edition of Xenopus Protocols:Post-Genomic Approachesexpands upon the previous edition with novel approaches inspired by Xenopus tropicalis and the sequencing of its genome. Xenopus tropicalis genomics and tractable...
Humana Press, 2006. — 429 p. — (Methods in Molecular Biology 333).
Technical innovations in the laboratory over the past ten years have greatly improved our understanding of the immunological mechanisms of transplanted organ rejection. In Transplantation Immunology: Methods and Protocols, leading experts in solid organ transplantation review the current status of the field and...
Humana Press, 2022. — 375 p. — (Methods in Molecular Biology 2458). — ISBN 978-1-0716-2139-4. This volume provides cutting-edge techniques to further the study chromatin biology. Chapters include both novel and well-established methods for the analysis of DNA-associated proteins, DNA methylation, three-dimensional chromatin interactions, deep sequencing-based tools, and data...
Humana Press, 2019. — 290 p. — (Methods in Molecular Biology 1901). This volume covers methods for determination of autoantibodies in rheumatic connective tissue diseases and organ-specific diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents,...
Humana Press, 2015. — 366 p. — (Methods in Molecular Biology 1348).
This extensive volume covers basic and advanced aspects of peptide antibody production, characterization and uses. Although peptide antibodies have been available for many years, they continue to be a field of active research and method development. For example, peptide antibodies which are dependent on...
New York: Humana Press, 2021. — 347 p. This detailed book covers methods for studying, producing, and analyzing therapeutic antibodies, measuring their concentration, developing neutralizing antibodies for them, and for predicting and monitoring their therapeutic efficacy and clinical effects. These biologics are the fastest growing pharmaceutical drug group and have had...
Humana Press, 1993. - 379 p. ISBN: 0896032264 Series: Methods in Molecular Biology, №14 A General Strategy for Glycoprotein Oligosaccharide Analysis. Analysis of Asparagine-Linked Oligosaccharides by Sequential Lectin-Affinity Chromatography. Sequence and Linkage Analysis of N- and O-Linked Glycans by Fast Atom Bombardment Mass Spectrometry. Gas Chromatography and Gas...
Humana Press, 1995. — 278 p. ISBN: 0896032973 Series: Methods in Molecular Biology, №46 Antibiotic Resistance Profiling Bacteriocin Typing Bacteriophage Typing The Analysis of Bacterial Proteins by SDS Polyacrylamide Gel Electrophoresis Lipopolysaccharide Chemotyping Multilocus Enzyme Electrophoresis Fast Atom Bombardment-Mass Spectrometry
Humana Press, 2019. — 273 p. — (Methods in Molecular Biology 1876). This volume provides an up-to-date, in-depth overview of the methods that have been applied to studying the complex metalloproteins at a molecular level. Chapters cover a wide range of approaches focusing on genetic, biochemical, spectroscopic, chemical methods, and theoretical calculations. Written in the highly...
New York: Humana, 2022. — 395 p. This volume provides a comprehensive compilation of protocols in T cell repertoire analysis, from the leading experts in the field, representing both well-established methods and cutting-edge advances. Chapters broadly cover the emerging new T cell subsets, sequencing technologies for capturing TCR repertoire, and computational tools for...
Springer, 2017. — 283 p. — (Methods in Molecular Biology 1617). This thorough volume provides an in-depth introduction to and discussion of microRNAs (miRs) and their targets, miR functions, and computational techniques applied in miR research, thus serving the need for a comprehensive book focusing on miR target genes, miR regulation mechanisms, miR functions performed in...
Humana Press, 2018. — 418 p. — (Methods in Molecular Biology 1754). This volume introduces the reader to the latest experimental and bioinformatics methods for DNA sequencing, RNA sequencing, cell-free tumour DNA sequencing, single cell sequencing, single-cell proteomics and metabolomics. Chapters detail advanced analysis methods, such as Genome-Wide Association Studies (GWAS),...
Humana Press, 2023. — 327 p. — (Methods in Molecular Biology 2695). — ISBN 978-1-0716-3345-8. This book analyzes liquid biopsy applications in cancer and other diseases. Chapters guide readers through the latest technologies and analysis methods for liquid biopsy,liquid biopsy in cancer, role of liquid biopsies in rheumatoid arthriti, cell-free circulating DNA profiling in...
Humana Press, 2023. — 327 p. — (Methods in Molecular Biology 2695). — ISBN 978-1-0716-3346-5. This book analyzes liquid biopsy applications in cancer and other diseases. Chapters guide readers through the latest technologies and analysis methods for liquid biopsy,liquid biopsy in cancer, role of liquid biopsies in rheumatoid arthriti, cell-free circulating DNA profiling in...
Humana Press, 2023. — 290 p. — (Methods in Molecular Biology 2683). This detailed volume presents validated and well-adapted procedures involving humanized and/or stem cell-based neural model systems that have proven helpful in better understanding the essential brain functions involved in the pathogenesis of brain disorders. The book explores the generation of multiple neural...
New York: Humana Press, 2015. — 535 p. — (Methods in Molecular Biology 1220). The book expands upon the previous edition with current, detailed reviews that cover topics of interest to mast cell neophytes and cognoscenti alike. With new chapters on methods for the enumeration of tissue mast cells and isolation of mature mast cells and mast cell progenitors from mammalian tissues,...
Humana Press, 2004. - 409 p. Series: Methods in Molecular Biology, №296 This collection of cutting–edge techniques for the study of the eukaryotic cell cycle and its key regulatory molecules includes overviews of cell cycle regulatory mechanisms in many major research organisms. Described in step-by-step detail, these readily reproducible methods enable fundamental research on...
Humana Press, 2015. — 237 p. Series: Methods in Molecular Biology, № 1255 This volume presents the most recent studies on mRNA polyadenylation in plants. Chapters are divided into three sections covering recent development of the use of bioinformatics tools in the field. Numerous molecular, biochemical, and methods used to characterize polyadenylation sites on a genome-wide scale....
Humana Press, 2011. - 447 p. - Methods in Molecular Biology, Volume 726. - Due to their unique size-dependent properties, nanomaterials have the potential to revolutionize the detection, diagnosis, and treatment of disease by offering superior capabilities compared to conventionally-used materials. Biomedical Nanotechnology: Methods and Protocols brings together experts from a...
Springer Science+Business Media, 2020. — 337 p. — (Methods in Molecular Biology 2103). — ISBN: 978-1-0716-0226-3. This book provides a variety of procedures for synthetically producing peptides and their derivatives, ensuring the kind of precision that is of paramount importance for successful synthesis. Numerous techniques relevant to drugs and vaccines are explored, such as...
New York: Humana Press, 2023. — 368 p. This detailed volume explores the development of technologies and protocols that are currently being used to understand the nature and activities of the plant cytoskeleton. A focus for many of the chapters is on sample preparation, as the quality of plant organ/tissue preparation, from single to multicellular samples, determines the...
Humana Press, 2018. - 556 p. - Volume 1701 of Methods in Molecular Biology. This volume provides comprehensive explanations and detailed examples of different antibody libraries, along with novel approaches for antibody discovery. The chapters in this book are divided into four sections: 1) construction of antibody libraries; 2) selection strategies for antibodies; 3)...
2nd edition. — Humana Press, 2023. — 598 p. — (Methods in Molecular Biology 2702). — ISBN 978-1-0716-3380-9. This second edition details new and updated methods on different antibody libraries, along with novel approaches for antibody discovery. Chapters focuses on the construction of antibody libraries, antibody expression, complementary approaches for antibody selection, and...
Humana Press, 1993. — 443 p. ISBN: 0896032396 Series: Methods in Molecular Biology, №21 Culturing and Biological Cloning of Trypanosoma brucei Culturing and Biological Cloning of Trypanosoma cruzi In Vitro Cultivation and Biological Cloning of Leishmania Simple In Vitro Cultivation of the Malaria Parasite Plasmodium falciparum (Erythrocytic Stages) Suitable for Large-Scale...
Humana, 2022. — 481 p. This volume provides state-of-the-art methodology to functionalize microtubules and construct microtubule-based materials. Chapters consists of six sections detailing modification of microtubule structures, observation and control of microtubule movement, material applications of microtubules in vitro, development of microtubule-binding molecules,...
Humana Press, 2005. — 225 p. — (Methods in Molecular Biology 311). — ISBN: 1-59259-957-5. History and Overview Pharmacogenomics: Historical Perspective and Current Status Functional Analysis of Gene Variation Transfection Assays With Allele-Specific Constructs: Functional Analysis of Udp-glucuronosyltransferase Variants Snapshot of the Allele-Specific Variation in Human Gene...
Springer, 2019. — 227 p. — (Methods in Molecular Biology 1968). This volume looks at the newest methods, materials, equipment, and technologies developed to study the cell biology of the pneumococcus. The chapters, which cover a wide range of topics, are organized into six parts: Part one discusses the cultivation of Streptococcus pneumoniae in vitro; Part Two talks about the...
Humana Press, 2001. — 514 p. ISBN 978-0-89603-759-5 Series: Methods in Molecular Biology, №171 Isolation of Proteoglycans from Cell Cultures and Tissues. (Masaki Yanagishita) Isolation of Proteoglycans from Tendon. (Kathryn G. Vogel and Julie A. Peters) Purification of Proteoglycans from Mineralized Tissues. (Neal S. Fedarko) Purification of Perlecan from Endothelial Cells....
Humana Press, 1993. - 260 p.ISBN: 9780896032545 Series: Methods in Molecular Biology, №28 About Nonradioactive Nucleic Acid Detection Isolation of DNA from Plants Isolation of High-Molecular-Weight DNA from Animal Cells Restriction Enzyme Digestion, Gel Electrophoresis, and Vacuum Blotting of DNA to Nylon Membranes Isolation of Plant RNA Isolation of Total and PolyA + RNA from...
Humana Press, 2004. — 408 p. Series: Methods in Molecular Biology, № 269 Recombinant vaccinia viruses have become important in recent years, both as protein expression vectors, and-in the age of bioterrorism-as powerful tools for developing new smallpox vaccines, diagnostics, and understanding of poxvirus pathogenesis. In Vaccinia Virus and Poxvirology: Methods and Protocols,...
Humana Press, 2018. — 175 p. Series: Methods in Molecular Biology, № 1763 This detailed volume presents a number of chapters that will aid researchers in jumping the hurdle preventing the popularization of advanced intravital imaging technology usage, namely the complicated experimental protocols. Leading researchers undertaking imaging studies in the field of bone and immune...
Humana Press, 2016 — 275p. — (Methods in Molecular Biology 1434) — ISBN: 978-1-4939-3652-6 (eBook), 978-1-4939-3650-2 (Hardcover).
Presenting topics from the basic application of molecular genetics to more complex gene expression analysis using different models of study, this detailed volume explores asthma through the lens of genetics, considered to play an essential role in...
Humana Press, 2020. — 288 p. — (Springer Protocols Handbooks). — ISBN: 978-1-0716-0615-5. This volume details the fundamentals of the CRISPR-Cas system, and its protocols illustrate advances in CRISPR-Cas techniques for efficient genome editing. Introductory chapters provide a wide horizon of CRISPR/Cas-based methods and applications. Additional chapters guide readers through...
Humana Press, 2021. — 397 p. — ISBN 978-1-0716-1656-7. This second volume provides new and updated methods detailing advancements in CRISPR-Cas technical protocols. Chapters guide readers through protocols on prime editing, base editing, multiplex editing, editing in cell-free extract, in silico analysis of gRNA secondary structure and CRISPR-diagnosis. Authoritative and...
New York: Humana, 2022. — 225 p. This volume discusses protocols that cover synthesis, screening by selection, and analysis of DNA-encoded chemical libraries (DEL). Chapters in this book focus on methods used to practice DEL technology and include solution phase library synthesis using a variety of chemistries; DNA encoding of chemical structure; design, preparation and...
Springer, 2016. — 345 p. — (Methods in Molecular Biology 1422). — ISBN 978-1-4939-3601-4. Желудочнокишечная Физиология и Болезни: Методы и Протоколы This volume provides a comprehensive collection of classical and cutting edge protocols and techniques to examine the normal development and physiological functions of the gastrointestinal system and to model the most common...
Humana Press, 2022. — 359 p. — (Methods in Molecular Biology 2515). — ISBN 978-1-0716-2408-1. This volume covers comprehensive methods on ways to assess structural and ultrastructural changes in the mitochondria, cytoskeleton, and microglia using state-of-the-art microscopy techniques including super-resolution imaging, electron microscopy, and ultra-high field MRI. The...
Humana Press, Springer Science + Business Media, 2008. — 251 p. Series: Methods in Molecular Biology, №437 In this concise and systematic book, a team of experts select the most important, cutting-edge technologies used in drug delivery systems. They take into account significant drugs, new technologies such as nanoparticles, and therapeutic applications. The chapters present...
New York: Humana Press, 2015. — 199 p. The book highlights the numerous molecular, cellular and in vivo tools now available to conduct human rhinovirus (HRV) research in an effort to increase understanding of the clinical disease caused by HRVs as well as the functions of its individual proteins and its replication. Human rhinoviruses are the major cause of common colds as well as...
Humana Press, 2015. — 414 p. — (Methods in Molecular Biology 1271). — ISBN: 978-1-4939-2330-4 (eBook), 978-1-4939-2329-8 (Hardcover). This volume explores the technological improvements in protein engineering, expression, purification, and crystallization of several rhodopsin photoactive intermediates, thus increasing our understanding of rhodopsin activation. The first...
Humana Press, 1995. - 394 p. ASIN B001F7490O Series: Methods in Molecular Biology, №34 Overview of Antibody Use in Immunocytochemistry. Purification of Antibodies Using Ammonium Sulfate Fractionation or Gel Filtration. Purification of Antibodies Using Ion-Exchange Chromatography Purification of Antibodies Using Affinity Chromatography Purification of Antibodies Using Protein...
Humana, 2024. — 284 p. — ISBN-13 : 978-1071640548 This volume details procedures relevant to all disciplines, with specific emphasis on challenging aspects of working with Bacterial Extracellular Vesicle (BEVs). Chapters are divided into four parts focusing on characterization of Bacterial Extracellular Vesicles, assessment of Bacterial Extracellular Vesicle Biological Function...
New York: Humana Press, 2023. — 221 p. This detailed volume focuses on iron homeostasis in plants, iron being an essential micronutrient that serves as a cofactor in numerous metabolic processes but is harmful in excess. Specifically, the content ranges from protocols to study the iron deficiency response, the interaction between root and microbes under iron deficient...
Humana Press, 2018. — 476 p. — (Methods in Molecular Biology 1767). — ISBN: 978-1-4939-7774-1 This detailed book explores the concepts and applications of epigenome editing, as presented by leading scientists in the field. Beginning with some general and topical reviews, the collection continues by covering the design of DNA-binding devices, optimization of the effector...
Second Edition. — Humana Press, 2023. — 373 p. — (Methods in Molecular Biology 2691). This second edition volume expands on the previous edition with an update on the broad spectrum of research models, techniques, and protocols used in laboratories by basic and clinical researchers. The chapters in this book are divided into two parts. Part One discusses the latest findings on...
Humana Press, 2013. — 541 p. Series: Methods in Molecular Biology, № 949 Microfluidic techniques are becoming widely incorporated into medical diagnostic systems due to the inherent advantages of miniaturization. In this volume researchers in the field detail methods and protocols covering subjects such as microfluidic device fabrication, on-chip sample preparation, diagnostic...
3rd edition. — Humana Press, 2020. — 274 p. — (Methods in Molecular Biology 2069). — ISBN: 978-1-4939-9848-7. This third edition volume expands on the previous editions with an update on the latest techniques used for the detection, genotyping, and investigating pathogenesis of Staphylococcus aureus in vitro and in vivo. The methods covered in this book mostly focus on routine...
New York: Humana, 2022. — 256 p. This volume provides a wide spectrum of multidisciplinary approaches for studying RNA structure and dynamics, including detailed accounts of experimental and computational procedures. Chapters guide readers through cryo-electron microscopy, crystallography, isothermal titration calorimetry, small angle X-ray scattering, single-molecule Förster...
Humana Press, 2017. — 435 p. — (Methods in Molecular Biology 1487). This volume provides a collection of techniques and approaches for the study of ERK signaling. It begins with a historical perspective of genetic and molecular discoveries, followed by chapters covering specific topics in a broad range of experimental systems, including in vitro assays of EGFR and ERK activities;...
2nd edition. — Humana Press, 2021. — 235 p. — (Methods in Molecular Biology 2170). — ISBN 978-1-0716-0742-8. This updated book covers a wide range of techniques on RNA extraction, detection, quantification, visualization, and genome-wide profiling, from conventional methods to state-of-the-art high throughput approaches. It includes detailed techniques to examine mRNAs, small...
New York: Humana Press, 2022. — 492 p. This volume covers the latest techniques that study the synthesis of melatonin, its receptor function, and its effects at the cellular and systemic level. The chapters are organized into three parts. Part One describes methods for the detection of melatonin and its biological derivatives in various biological samples, the manipulation of...
New York: Humana Press, 2006. — 508 p. Although Xenopus oocytes are very hardy and robust in terms of experimentation and offer unusually simple and inexpensive opportunities for the delivery of mRNA to the cytoplasm and DNA to the nucleus, they are underutilized as an experimental model. In Xenopus Protocols: Cell Biology and Signal Transduction, hands-on researchers describe...
2nd Edition. - Humana Press, 2018. - 397 p. - Volume 1683 of Methods in Molecular Biology. This second edition details some of the recent trends in HCS/HCA/HCI. New and updated chapters guide readers through methods that utilize reagents and kits that have been developed to measure cells and subpopulation classifications, control measures, overviews of the data handling issues...
Humana Press, 1993. — 274 p. ISBN: 0896032329 Series: Methods in Molecular Biology, №22 This superb survey of the possible applications of physiochemical techniques to the analysis of biological macromolecules is designed for the practicing biochemist or biologist who wants to use the technique but lacks the experience. The contributors emphasize practical aspects, such as...
Humana Press Inc.,1993. – 406 p. – ISBN: 9780896032156. Series: Methods in Molecular Biology, №17 The main strength of this volume...is the extensive attention paid to the pragmatic and mundane issues that are only gleaned with difficulty from the literature, yet are absolutely critical for success. Those contemplating initiating studies employing these physical methods would...
Humana Press, 1995. — 457 p. ISBN: 089603321X Series: Methods in Molecular Biology, №49 In Plant Gene Transfer and Expression Protocols, leading experts present the latest step-by-step recipes for introducing genes into model and agricultural plants and for studying gene activity using reporter enzyme assays, RNA techniques, and immunological methods. The book contains not only...
Humana Press, 2014. — 297 p. Series: Methods in Molecular Biology, № 1157 Listeria monocytogenes is still a major threat to public health. A new book in the Methods in Molecular Biology series addresses its titular pathogen with protocols and methodologies used in research to gain a better understanding of Listeria at a molecular level. The topics covered include sampling in order...
Jorrin-Novo J.V., Valledor L., Castillejo Sanchez M.A., Rey M.-D. — Springer, 2020. — 432 p. — (Methods in Molecular Biology 2139). — ISBN: 1071605275. This third edition details recent advances made in the field of proteomics and the application to plant biology and translational research. In recent years, improvements have been made to protocols on high-throughput proteomics...
Humana Press, 2013. — 335 p. — (Methods in Molecular Biology 1041). Key discoveries concerning the different biological functions of microglia in health and disease have attracted scientists from various fields. In this volume, expert researchers in the field detail methods for selection of the key cellular, molecular and biochemical techniques that are used in studying the many...
Springer, 2024. — 607 p. This second edition details new and updated protocols that cover techniques used to study secretion systems. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and...
Humana Press, 2017. — 521 p. — (Methods in Molecular Biology 1615). — ISBN: 978-1-4939-7031-5. This volume details protocols that cover the broad arsenal of techniques used to study a secretion system from A to Z. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging...
Humana Press, 2015. — 314 p. — ISBN: 1493931059, 9781493931057 Series: Methods in Molecular Biology, № 1362 This valuable collection aims to provide a collection of frequently used statistical methods in the field of proteomics. Although there is a large overlap between statistical methods for the different ‘omics’ fields, methods for analyzing data from proteomics experiments...
Humana Press, 2013. — 325 p. Series: Methods in Molecular Biology, № 964 Dopamine, a catecholamine transmitter, plays a number of vital physiological roles in the brain and body, and, in recent years, studies on the role of dopamine in disease have opened new avenues of research and discovery. In this volume experts and key figures within the field provide detailed protocols on...
Humana, 2021. — 398 p. This volume explores methods and protocols for detecting epistasis from genetic data. Chapters provide methods and protocols demonstrating approaches to identify epistasis, genetic epistasis testing, genome-wide epistatic SNP networks, epistasis detection through machine learning, and complex interaction analysis using trigenic synthetic genetic array...
Humana Press, 2019. — 307 p. — (Methods in Molecular Biology 2098) — ISBN: 978-1-07-160206-5 This volume focuses on various methods used by researchers to study mucosal-associated invariant T (MAIT) cells in the aspects described below. The chapters are organized into Four Parts: part One looks at the function and importance of MAIT cells in health and disease. It covers...
ITexLi, 2018. — 121 p. — ISBN 1789237912 9781789237917 1838816410 9781838816414. This book focuses on the transcriptional and post-transcriptional gene regulations and presents a detailed portrait of many novel aspects related to highlighting the importance of key TFs in some vital biological processes, the role of certain TFs to control some infectious diseases, the role of...
Humana Press, 2005. — 300 p. — (Methods in Molecular Biology 302). — eISBN 1-59259-903-6. Elispot Assay Setup And Data Analysis The Elispot Assay: A Personal Retrospective Chemistry And Biology Of The Elispot Assay Membranes And Membrane Plates Used In Elispot Standardization And Validation Issues Of The Elispot Assay A Cell-Detachment Solution Can Reduce Background Staining In...
2nd Edition. - Humana Press, 2017. — 288 p. — (Methods in Molecular Biology 1554). This second edition volume provides detailed protocols that address the challenges of signal-transduction IHC. This book delves into chapters that discuss the nature of signal transduction phenomena and approaches to making phosphor-specific antibodies, as well as numerous bona fide methods methods...
Humana Press, 2019. — 194 p. — (Methods in Molecular Biology 1887). Isolation and Identification of Lactic Acid Bacteria from Environmental Samples Basic Antibacterial Assay to Screen for Bacteriocinogenic Lactic Acid Bacteria and to Elementarily Characterize Their Bacteriocins Assaying d-Body Amino Acids as d-Alanine and Amino Acid Racemase (AARase) Activity Using NADH...
ITexLi, 2021. — 99 p. — ISBN 1-83969-121-2 978-1-83969-121-8 1-83969-122-0 978-1-83969-122-5. This book examines the role of RBCs in immunology. Chapters cover such topics as an iron deficiency in erythrocytes, the modulation of oxidative stress (OS) in erythrocytes in bacterial and viral infections, using human foetal astrocytes (HFAs) as an experimental model to measure early...
Humana Press, 2022. — 566 p. — (Methods in Molecular Biology 2429). — ISBN 978-1-0716-1978-0. This volume explores the fields of stem cell biology, regenerative medicine, and cancer biology. The chapters in this book cover topics such as embryonic stem cells (ES) and induced pluripotent cells (iPS); ES/iPSC derived tissue stem cells; tissue resident stem cells; assays for...
Humana Press, 2002. — 322 p. —— (Methods in Molecular Biology 194). — ISBN: 0-89603-678-2 The majority of all proteins undergo posttranslational modifications that significantly alter theirphysical and chemical properties, including their folding and conformation distribution, their stability, and, consequently, their activity and function. In Posttranslational Modifications of...
Humana Press, 2013. — 353 p. Series: Methods in Molecular Biology, № 1006 Microsatellites or simple sequence repeats (SSRs) have become the markers of choice for a variety of molecular studies because of their versatility, operational flexibility, and lower cost than other marker systems. The book brings together experts in the field to cover this significant area of research....
Humana Press, 2013. — 229 p. Series: Methods in Molecular Biology, № 1036 Embryonic stem cells and adult stem cells are the two major types of stem cells that have been used for experimental and clinical studies. Embryonic stem cells are totipotent cells that have the capability to differentiate into any type of cell in the body. In this volume expert researchers in the field...
ITexLi, 2024. — 157 p. — ISBN 183769009X 9781837690091 1837690081 9781837690084 1837690103 9781837690107. With the support of this comprehensive guide, discover the world of cutting-edge biosensing technological advancements. Prominent specialists explore the theoretical underpinnings and real-world applications of wearable sensors, solid-state sensors, electrochemical sensors,...
New York: Humana, 2022. — 437 p. This detailed volume explores perspectives and methods using cell-free expression (CFE) to enable next-generation synthetic biology applications. The first section focuses on tools for CFE systems, including a primer on DNA handling and reproducibility, as well as methods for cell extract preparation from diverse organisms and enabling...
Humana Press, 2018. — 553 p. — (Methods in Molecular Biology). — ISBN: 1493977768, 9781493977765. This volume explores and explains how digital PCRs (dPCRs) help in the study of numerous topics, such as infectious diseases, evolution of cancer and treatment responses, somatic mosaicism, genome editing and cell therapy, and food testing for GMOs and pathogens. Written in the...
2nd edition. — Humana Press, 2023. — 289 p. — (Methods in Molecular Biology 2620). — ISBN 978-1-0716-2941-3. This second edition provides updated and new chapters that detail comprehensive overview of all the existing methods on analyzing protein arginylation. Chapters guide readers through the early methods utilizing crude protein preparations and whole-cell assays to the...
Humana Press, 2023. — 377 p. — (Methods in Molecular Biology 2660). — ISBN 978-1-0716-3162-1. This thorough volume explores recent advances that have revolutionized the field of precision oncology. The chapters, contributed by experts in the areas of cancer systems and integrative biology, provide hands-on guidance toward developing tools to monitor spatial and temporal changes...
Humana Press, 2023. — 899 p. — (Methods in Molecular Biology 2660). — ISBN 978-1-0716-3163-8. This thorough volume explores recent advances that have revolutionized the field of precision oncology. The chapters, contributed by experts in the areas of cancer systems and integrative biology, provide hands-on guidance toward developing tools to monitor spatial and temporal changes...
Springer, 2021. — 322 p. — (Methods in Molecular Biology, 2231). — ISBN 978-1-0716-1035-0. This volume is a collection of protocols to install and run tools for calculation and visualiza- tion of multiple sequence alignment (MSA) and other analyses related to MSA. Following the policy of Methods in Molecular Biology, each chapter basically consists of a brief back- ground and a...
2nd edition. — Humana Press, 2023. — 380 p. — (Methods in Molecular Biology 2698). — ISBN 978-1-0716-3353-3. This second edition details protocols that analyze and explore gene regulatory networks (GRNs). Chapters guide readers through experimental techniques used to study genes and their regulatory interactions in plants, and computational approaches used for the integration...
Humana Press, 2012. - 435 p. Over the last decade Life Science has undergone an accelerated evolution, culminating in the -omics era characterized by the development of a multitude of high throughput methods that are becoming more routinely applied in biochemistry labs. In Functional Genomics: Methods and Protocols, Second Edition expert researchers in the field detail many of...
3rd Ed. — Humana Press, 2017. — 385 p. — (Methods in Molecular Biology). — ISBN10: 1493972308, 13 978-1493972302. Reflecting developments in genome editing, this third edition volume fully updates a collection of key techniques for the study of functional genomics. The book is broken up into sections on bioinformatics, DNA, RNA, and protein analysis, as well as a closing...
Humana Press, 2012. — 240 p. — (Methods in Molecular Biology 865). Reports of influenza-like illnesses date back to the Middle Ages, and outbreaks of influenza likely afflicted humans long before that. Over the last half century, influenza virus research has led to the development of two classes of antivirals – ion channel and neuraminidase inhibitors. Recently, a method of the...
Humana Press, 2017. - 284 p. Volume 1500 of Methods in Molecular Biology. This detailed volume presents a comprehensive technical overview of DNA nanotechnology with an emphasis on 3D DNA nanostructure design and applications. Coverage spans from basic design principles for DNA and RNA nanostructures to their cutting-edge applications in a variety of fields, with the book...
Humana Press, 2017. — 234 p. Series: Methods in Molecular Biology, № 1549 This thorough book covers the most recent proteomics techniques, databases, bioinformatics tools, and computational approaches that are used for the identification and functional annotation of proteins and their structure. The most recent proteomic resources widely used in the biomedical scientific...
2nd Edition. — Springer Science+Business Media, New York, USA, 2017. — 491 p. — (Methods in Molecular Biology 1525) — ISBN: 1493966200 This second edition provides updated and expanded chapters covering a broad sampling of useful and current methods in the rapidly developing and expanding field of bioinformatics. Bioinformatics, Volume I: Data, Sequence Analysis, and Evolution,...
2nd ed. — Humana Press, 2017. — 422 p. — (Methods in Molecular Biology). — ISBN10: 1493966111. — ISBN13: 978-1493966110. This second edition provides updated and expanded chapters covering a broad sampling of useful and current methods in the rapidly developing and expanding field of bioinformatics. Bioinformatics, Volume II: Structure, Function, and Applications, Second...
Humana Press, 1995. - 301 p. ISBN: 0896032981 Series: Methods in Molecular Biology, №41 This collection of self-contained, "hands-on" descriptions of established laboratory protocols concentrates on receptor-mediated cell signaling, with particular attention devoted to those receptors that are part of the G-protein-linked superfamily. It offers those already working in the...
Humana Press, 1992. - 338 p. ISBN: 0896032132 Series: Methods in Molecular Biology Series , №11 Thiophilic Adsorption Chromatography. Immobilized Metal Ion Affinity Chromatography. Histidine Ligand Affinity Chromatography. Boronic Acid Matrices for the Affinity Purification of Glycoproteins and Enzymes. Calcium-Dependent Hydrophobic Interaction Chromatography. Lectin Affinity...
Humana Press, 2015. — 413 p. — (Methods in Molecular Biology 1249). — ISBN: 149392012X, 9781493920129. Includes cutting-edge methods and protocols for researching cervical cancer and high-risk HPV types Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Representing the most relevant procedures and...
Humana Press, 2011. - 336 p. Series: Methods in Molecular Biology, №671 In Biological Microarrays: Methods and Protocols, expert researchers explore exciting new developments in the field, providing a comprehensive approach to biological microarrays that conveys not only the state-of-the-art fundamentals, but also includes applications of the most innovative methods....
Humana Press, 2016 — 408p. — (Methods in Molecular Biology 1408) — ISBN: 978-1-4939-3512-3 (eBook), 978-1-4939-3510-9 (Hardcover). This volume provides researchers with up-to-date protocols and takes a close look at current research and promising applications. Optogenetics: Methods and Protocols contains a collection of recently developed technical protocols on optogenetic...
Humana Press, 2014. — 297 p. Series: Methods in Molecular Biology, № 1150 The book collects techniques used to increase our understanding of the underlying transcriptional programs of stem cells that promote self-renewal and differentiation. The volume opens with a section on next-generation sequencing library preparation and data analysis. Continuing with a collection of...
3rd Edition. - Springer, 2014. - 267 p. - Volume 1137 of Methods in Molecular Biology. - Protein Structure Prediction, Third Edition expands on previous editions by focusing on software and web servers. With new chapters that provide instructions on how to use a computational method with examples of prediction by the method. Written in the highly successful Methods in Molecular...
Humana Press, 2023. — 333 p. — (Methods in Molecular Biology 2651). — ISBN 978-1-0716-3083-9. This detailed volume compiles key methods and techniques used to establish some of the structural and functional aspects of Z-form nucleic acids. Beginning with chapters on characterizing Z-DNA, the book continues by exploring structure determination techniques, gene- and...
4th edition. — Humana Press, 2022. — 491 p. — (Methods in Molecular Biology 2524). — ISBN 978-1-0716-2452-4. This detailed collection explores recent advances in molecular imaging techniques involving bioluminescence, currently employed in biolaboratories around the world. Volume 1 delves into techniques for the establishment of luciferins and luciferases, basic in vitro and in...
Humana Press, 2021. — 447 p. — ISBN 978-1-0716-1257-6. This detailed book highlights recent advances in molecular imaging techniques and protocols, designed to be immediately applicable in global bio-laboratories. The chapters are categorized into seven major groups according to the reporter materials, such as imaging with passive optical readouts, activatable bioluminescent...
Humana Press, 2022. — 522 p. — (Neuromethods 179). — ISBN 978-1-0716-2082-3. This volume explores the latest techniques used to study brain function and pathophysiology of major depressive disorder (MDD), and includes suggestions of new therapeutic approaches for the treatment of MDD. The chapters into this book are organized into five parts. Part One discusses advanced...
Humana Press, 2012. - 329 p. - Super secondary structure(SSS) helps to understand the relationship between primary and tertiary structure of proteins. In Protein Supersecondary Structure: Methods and Protocols expert researchers in the field detail the usefulness of the study of super secondary structure in different areas of protein research. This is done through four main...
3rd ed. — Humana Press, 2025. — 410 p. This new edition delves into the latest developments in the field and new techniques used to study secondary and supersecondary structures (SSS) in proteins. Beyond the tremendous advances in the field from the AI-based AlphaFold algorithm, researchers continue to untangle how specific structures and protein folds come to be, and these...
Humana Press, 2017. — 289 p. — (Methods in Molecular Biology 1497). This volume aims to present a representative cross-section of modern experimental approaches relevant to Plant Hormone Biology, ranging from relatively simple physiological to highly sophisticated methods. Chapters describe physiological, developmental, microscopy-based techniques, measure hormone contents, and...
Humana Press, 2013. — 475 p. Series: Methods in Molecular Biology, № 974 Biological membranes are the essential structuring elements of all living cells. Many enzymatic reactions take place at the membrane-water interface. To gain detailed insight into membrane properties, it is therefore of great importance to understand the complex nature of the interactions of membrane...
2nd ed. — Humana Press, 2024. — 360 p. This second edition volume expands on the previous edition with updates on the latest methods, resources, and studies concerning analysis and prediction of various structural and functional aspects of proteins and ncRNAs. The chapters in this book cover topics such as secondary structure characterization and prediction; the use and impact...
Humana Press, 2015. — 230 p. — (Methods in Molecular Biology 1289). — ISBN: 978-1-4939-2486-8 (eBook), 978-1-4939-2485-1 (Hardcover). This volume covers the techniques necessary for a successful fragment-based drug design project, beginning from defining the problem in terms of preparing the protein model, identifying potential binding sites, and the consideration of various...
Humana Press, 1994. - 425 p. ISBN: 0896032566 Series: Methods in Molecular Biology, №30 DNase I Footprinting Footprinting with Exonuclease III Hydroxyl Radical Footprinting Hydroxyl Radical Interference 1,10-Phenanthroline-Copper Ion Nuclease Footprinting of DNA-Protein Complexes in Situ Following Mobility-Shift Electrophoresis Assays Identification of Protein-DNA Contacts with...
Springer Science+Business Media, 2016. — 769 p. — (Methods in Molecular Biology 1462). — ISBN: 1493938142 This volume discusses experimental brain injury models that contain valuable information carefully chosen to widen the researchers’ horizon about neurotrauma. Injury Models of Central Nervous System: Methods and Protocols contains relevant experimental design approaches...
Springer Science+Business Media, 2016. — 769 p. — (Methods in Molecular Biology 1462). — ISBN: 1493938142. This volume discusses experimental brain injury models that contain valuable information carefully chosen to widen the researchers’ horizon about neurotrauma. Injury Models of Central Nervous System: Methods and Protocols contains relevant experimental design approaches...
2nd Ed. — Humana Press, 2017. — 423 p. — (Methods in Molecular Biology 1598). — ISBN10: 1493969501, ISBN13: 978-1493969500. Preview ebook This new edition provides updated and novel protocols of neuroproteomics methods that encompass both global-scale as well as targeted and specialized topics, which are timely additions for the molecular and phenotypic analysis of the central...
Humana Press, 2013. — 353 p. Series: Methods in Molecular Biology, №951 Glycosylation is the most abundant post-translational modification of proteins. Estimates vary widely, but a common assessment is that upwards of 50% of eukaryotic proteins are modified by some type of glycan. In Mass Spectrometry of Glycoproteins: Methods and Protocols, expert researchers in the field...
Humana Press, 2004 — 355 p. — e-ISBN: 1-59259-804-8 Series: Methods in Molecular Biology, № 277 Mouse Models of Triplet Repeat Diseases Analysis of triplet repeat DNAs and RNAs Analysis of Triplet Repeat Replication by Two-Dimensional Gel Electrophoresis Genetic Analysis for Triplet Repeat Instability in Yeast Detection and Isolation of Trinucleotide Repeat Expansions Using the...
Humana Press, 2021. — 330 p. — ISBN 978-1-0716-1440-2. This volume explores the latest engineering methods of mammalian cells that are useful for controlling the performance of engineered mammalian cells for future cell-based therapeutics and for better understanding of complex biological systems. The chapters in this book are organized into five parts. Part One described...
Humana Press, 2021. — 330 p. — ISBN 978-1-0716-1440-2. This volume explores the latest engineering methods of mammalian cells that are useful for controlling the performance of engineered mammalian cells for future cell-based therapeutics and for better understanding of complex biological systems. The chapters in this book are organized into five parts. Part One described...
Humana, 2024. — 337 p. Since the publication of the previous edition, the use of 3D cell and organoid cultures has become more widespread across laboratories. This second edition volume expands on the previous edition with discussions about the latest organoid models developed for many more organs; new hydrogels and devices for 3D culture; and the organoid systems that have...
Humana Press, 2012. — 255 p. — (Methods in Molecular Biology 904). Stem cell mobilization represents a transient increase in the levels of circulating stem and progenitor cells. In this volume expert researchers in the field detail cell mobilization methodology and recent developments in the field for basic and biomedical research community. Specifically clinical hematopoietic...
Humana Press, 2013. — 312 p. — (Methods in Molecular Biology 1039). — ISBN 978-1-62703-534-7. In Nucleic Acid Chemistry: Methods and Protocols, expert researches in the field detail techniques and approaches for the detection of DNA and RNA. These techniques include the recovery of trace amounts of DNA for amplification and analysis, new qPCR chemistries, new application of...
Humana Press, 2014. — 279 p. Series: Methods in Molecular Biology, № 1171 Mitogen-activated protein kinases (MAPKs) are versatile phosphorylating enzymes which regulate multiple proteins involved in gene expression, cell architecture, plant development and reaction to diverse abiotic and biotic factors. The main aim of Plant MAP Kinases: Methods and Protocols is to provide...
Humana Press, 2013. — 283 p. Series: Methods in Molecular Biology, № 1024 microRNAs (miRNAs) are small non-coding RNAs that regulate various biological phenomena, such as development and homeostasis. The dysregulation of miRNA leads to disease progression, particularly of cancer. In this volume expert researchers in the field detail recent advances in the isolation,...
2nd Edition. — Springer Science+Business Media, New York, 2017. — 256 p. — (Methods in Molecular Biology 1456) — ISBN10: 1489977066 This volume provides a variety of protocols to analyze various epigenetic changes, including differential expression of non-coding RNAs, changes in DNA methylation, and histone modifications in plants. Chapters detail protocols with different...
Humana, 2020. — 934 p. The edition details methods to study intrinsically disordered proteins (IDPs) including recent topics such as extremely high-affinity disordered complexes, kinetics that evade established concepts, liquid-liquid phase separation, and novel disorder-driven allosteric mechanisms. Written in the highly successful Methods in Molecular Biology series format,...
Humana Press, 2017. — 403 p. — (Methods in Molecular Biology 1510). This detailed collection covers how the biological functions of histone deacetylases (HDACs) and histone acetyltransferases (HATs) can be detected in various experimental settings, both in vivo and in vitro. The book also covers the generation and specificity of deacetylase inhibitors and how such agents can be...
2nd edition. — Humana Press, 2023. — 508 p. — (Methods in Molecular Biology 2589). — ISBN 978-1-0716-2787-7. This fully updated edition provides a series of methods for how best to assess functions of histone deacetylases and acetyltransferases. The disease-relevance of dysregulated protein deacetylation by overexpressed or aberrantly activated histone deacetylases has spurred...
Humana Press, 2017. — 279 p. — (Methods in Molecular Biology 1499). This volume provides an overview of the field and practical hints for vaccinologists in academia and industry. Chapters provide protocols on self-replicating RNA vectors, non-replication mRNA vectors, adjuvantation and delivery, and preclinical and clinical development. Written in the highly successful Methods in...
Humana Press, 2019. — 279 p. — (Methods in Molecular Biology 1878). This volume covers a wide variety of state of the art cancer-related methods and tools for data analysis and interpretation. Chapters were designed to attract a broad readership, ranging from active researchers in computational biology and bioinformatics developers, clinical oncologists, and anti-cancer drug...
Humana Press, 2022. — 321 p. — (Methods in Molecular Biology 2424). — ISBN 978-1-0716-1955-1. This detailed volume provides a robust set of methods to understand variation between patients with ovarian cancers, in vitro models to better study different stages of the disease, and in vivo models to test therapies. Beginning with clinical perspectives to orient basic scientists,...
2nd edition. — Humana Press, 2024 — 434 p. — (Methods in Molecular Biology 2772). — ISBN 978-1-0716-3709-8. This second edition provides new and updated methods detailing techniques and state-of-the-art approaches on the structure and function of plant endoplasmic reticulum (ER). Chapters guide readers through modern microscopy techniques, software protocols, purification, and...
2nd edition. — Humana Press, 2024 — 434 p. — (Methods in Molecular Biology 2772). — ISBN 978-1-0716-3710-4. This second edition provides new and updated methods detailing techniques and state-of-the-art approaches on the structure and function of plant endoplasmic reticulum (ER). Chapters guide readers through modern microscopy techniques, software protocols, purification, and...
Humana Press, 2013. — 255 p. Series: Methods in Molecular Biology, № 1020 Recently, many ground-breaking steps have been made towards better understanding NO/cGMP/PKG pathways, its components, substrates, and their localization within a given cell. These advances were only possible due to the development of sophisticated new techniques in the field. In this volume expert...
New York: Humana, 2023. — 266 p. This volume provides a comprehensive collection of experimental protocols for investigating ferroptosis in different systems, including cultured cells, animal models, and human tissues. The techniques covered in this book look at various aspects of ferroptosis ranging from the detection of lipid peroxidation to the measurement of glutathione...
New York: Humana, 2023. — 266 p. This volume provides a comprehensive collection of experimental protocols for investigating ferroptosis in different systems, including cultured cells, animal models, and human tissues. The techniques covered in this book look at various aspects of ferroptosis ranging from the detection of lipid peroxidation to the measurement of glutathione...
Humana Press, 2024. — 230 p. — (Methods in Molecular Biology 2769). — ISBN 978-1-0716-3693-0. This volume discusses the latest advancements in modern methodologies used to study liver carcinogenesis. The first half of this book describes pertinent preclinical models of hepatocellular carcinoma (HCC) and cholangiocarcinoma (CCA), established either through orthotopic induction...
Humana Press, 2024. — 230 p. — (Methods in Molecular Biology 2769). — ISBN 978-1-0716-3694-7. This volume discusses the latest advancements in modern methodologies used to study liver carcinogenesis. The first half of this book describes pertinent preclinical models of hepatocellular carcinoma (HCC) and cholangiocarcinoma (CCA), established either through orthotopic induction...
Humana Press, 2015. — 284 p. Series: Methods in Molecular Biology №1347. This volume mirrors the holistic feature of whole genome amplification (WGA) technology by combining reviews, detailed basic methods and advanced sample workflows. The first part of the book covers an overview of the development of WGA techniques throughout recent years including general considerations on...
Humana Press, 2023. — 247 p. — (Methods in Molecular Biology 2658). — ISBN 978-1-0716-3154-6. This detailed volume aims to elucidate the molecular mechanisms that underlie antifungal resistance. The book highlights methods to identify and characterize antifungal activity, to define and characterize strains with altered responses to antifungal drugs, to investigate the genetic...
Humana Press, 2022. — 316 p. — (Methods in Molecular Biology 2523). — ISBN 978-1-0716-2448-7. This volume presents a collection of protocols to study effector-triggered immunity (ETI) in both plants and animals from eminent groups in the field. The chapters in this book cover topics such as genetic manipulation of plant and animal pathogens, host cells, and the analysis of key...
Humana Press, 2022. — 691 p. — (Methods in Molecular Biology 2523). — ISBN 978-1-0716-2449-4. This volume presents a collection of protocols to study effector-triggered immunity (ETI) in both plants and animals from eminent groups in the field. The chapters in this book cover topics such as genetic manipulation of plant and animal pathogens, host cells, and the analysis of key...
Humana, 2024. — 377 p. This volume explores the latest technologies used to study the co-existence of neuronal and neuron-glia synapses in the brain. The chapters in this volume are organized into three parts. Part One covers recent advancements in the technical approaches for studying glutamatergic signalling between neurons. Part Two highlights approaches to study the...
2nd Edition. - Springer, 2015. - 474 p. Series Methods in Molecular Biology, Vоlume 1215 Molecular Modeling of Proteins, Second Edition provides a theoretical background of various methods available and enables non-specialists to apply methods to their problems by including updated chapters and new material not covered in the first edition. This detailed volume opens by...
New York: Humana Press, 2015. — 369 p. — (Methods in Molecular Biology 1290). This detailed volume focuses on best practices and conditions for maintaining the most commonly used salamander species in the laboratory. Salamanders in Regeneration Research: Methods and Protocols guides readers through experimental manipulations in vivo and in vitro, respectively. With methods on...
N Y: Humana Press, 2014. — 288 p. Series: Methods in Molecular Biology, № 1159 Biomedical Literature Mining, discusses the multiple facets of modern biomedical literature mining and its many applications in genomics and systems biology. The volume is divided into three sections focusing on information retrieval, integrated text-mining approaches and domain-specific mining methods....
Humana Press, 2014 — 3rd Ed. — 799p. — (Methods in Molecular Biology 1117) — ISBN: 978-1-62703-776-1 (eBook), 978-1-62703-775-4 (Hardcover). This third edition of Electron Microscopy: Methods and Protocols expands upon the previous editions with current, detailed protocols on biological and molecular research techniques based on TEM and SEM as well as other closely related...
Humana Press, 2019. - 439 p. - Volume 1956 of Methods in Molecular Biology. This second edition volume expands on the previous edition with descriptions of recent developments in the field. The new and updated chapters discuss the latest approaches for studying single cell RNA sequencing, whole genome sequencing data, CRISPR/Cas9-mediated gene knockout, protein interactions by...
Humana Press, 2013. — 329 p. Series: Methods in Molecular Biology, № 971 Lymphomas are lymphoid malignancies derived from B or T lymphocytes, and their study has been and still is paradigmatic for many aspects of cancer research. This volume presents and discusses key methods that are used in lymphoma research, partly specific for lymphoma research but often adaptable to the...
Humana Press, 2025. — 499 p. Since the publication of the second edition of this book on methods in lymphoma research in 2019, a variety of additional methods have been developed or further refined that can now be used to study the molecular biology of lymphomas. Therefore, this new edition includes several new chapters that describe approaches for single-cell B-cell and T-cell...
Humana Press, 2015 — 386p. — (Methods in Molecular Biology 1314) — ISBN: 978-1-4939-2718-0 (eBook), 978-1-4939-2717-3 (Hardcover). This detailed volume provides methods and techniques for detection after blotting. Chapters guide readers through a number of variations on the theme of protein transfer to solid support followed by detection, presenting adaptations of traditional...
Humana Press, 2015 — 509p. — (Methods in Molecular Biology 1312) — ISBN: 978-1-4939-2694-7 (eBook), 978-1-4939-2693-0 (Hardcover).
This volume covers past and present western blot techniques, such as diffusion blotting, slice blotting, blotting of high and low molecular weight proteins, single cell blotting and automated blotting. Written in the highly successful Methods in...
Humana Press, 2018. — 283 p. — (Methods in Molecular Biology). — ISBN10: 1493987445, 13 978-1493987443. This volume expands upon the collection of techniques published in Protein Electrophoresis: Methods and Protocols (2012) with more practical and reproducible methods to study protein gel detection and imaging. The chapters in this book cover topics such as coomassie-brilliant...
New York: Humana Press, 2012. — 269 p. Protein and lipid kinases are often the master regulators of cell signaling in eukaryotic systems. The human genome codes for more than 500 of these enzymes and their misregulation has been shown to be involved in the onset and progression of many diseases including cancer and inflammation. Therefore, small molecule kinase inhibitors have...
Humana Press, 2002. — 288 p.
Series: Methods in Molecular Biology, №212
Pui-Yan Kwok, MD, PhD, has assembled a comprehensive collection of readily reproducible techniques for the difficult process of single nucleotide polymorphisms (SNP) discovery and genotyping. These cutting-edge protocols for mutation/SNP detection utilize denaturing high-performance liquid chromatography...
Humana Press, 2014 — 555p. — (Methods in Molecular Biology 1129) — ISBN: 978-1-62703-977-2 (eBook), 978-1-62703-976-5 (Hardcover). Proteins are the most diverse group of biologically important substances. With the recent technological advances in the genomics area and the efforts in proteomics research, the rate of discovery for new proteins with unknown structure and function...
Humana Press, 2018. - 301 p. - Volume 1686 of Methods in Molecular Biology. This detailed volume explores methods and protocols that aim to increase our understanding of how cells enter a quiescent state during homeostasis and how cells exit quiescence and re-enter differentiating cell divisions to restore damaged tissues, essential for developing new approaches in regenerative...
2nd ed. — Humana Press, 2025. — 502 p. This second edition details a collection of state-of-art methods including identification of novel ncRNAs and their targets, functional annotation and disease association in different biological contexts. Chapters guide readers through an overview of disease-specific ncRNAs, computational methods and workflows for ncRNA discovery,...
Humana Press, 2019. — 454 p. — (Methods in Molecular Biology 1912). This volume details a collection of state-of-art methods including identification of novel ncRNAs and their targets, functional annotation and disease association in different biological contexts. Chapters guide readers through an overview of disease-specific ncRNAs, computational methods and workflows for...
Humana Press, 2013. — 303 p. Series: Methods in Molecular Biology, № 997 Human pluripotent stem cells such as human embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC) with their unique developmental plasticity hold immense potential as cellular models for drug discovery and in regenerative medicine as a source for cell replacement. While hESC are derived from...
Humana Press, 2023. — 269 p. — (Methods in Molecular Biology 2534). This detailed volume presents status and management protocols with the objective of identifying proper guidelines and materials for research related to the most common malignant neoplasm in the thyroid gland, papillary thyroid carcinoma. After an introduction, the book explores diagnostic approaches and initial...
Third Edition, Humana Press, 2012. — 359 p. — ISBN: 1627030859 Series: Methods in Molecular Biology, №937 The regulation of intracellular Ca 2+ has continued to be a powerful area of study since the publication of the first and second editions of Calcium Signaling Protocols, and the developments in the field have also, naturally, continued. With the third edition, expert...
2nd ed. — Humana Press, 2006. — 225 p. — ISBN: 1-59259-949-4 Series: Methods in Molecular Biology, № 312 Fluorescent Measurement of [Ca 2+ ]c: Basic Practical Considerations Measurement of [Ca 2+ ]i in Whole Cell Suspensions Using Fura-2 Measurement of [Ca 2+ ]i in Cell Suspensions Using Indo-1 Confocal Microscopy: Theory and Applications for Cellular Signaling Single-Cell and...
Springer, 2022. — 607 p. — (Methods in Molecular Biology 2453). — ISBN 978-1-0716-2115-8. The immunogenetics domain has rapidly evolved in the last ten years or so, mainly through the introduction of high-throughput technologies. With these new technologies, unprecedented insight into the adaptive immune receptor repertoire could be obtained with much more sequencing depth and...
2nd edition. — Humana Press, 2019. — 270 p. — (Methods in Molecular Biology 1872). — ISBN: 978-1-4939-8822-8. This second edition provides detailed LC-MS(/MS) procedures for the analysis of compounds of clinical and toxicological significance. Chapters detail new and updated methods for analyzing drugs focusing on advances in technology, alternate matrices, and rapidly-changing...
2nd edition. — Humana Press, 2023. — 258 p. — (Methods in Molecular Biology 2655). — ISBN 978-1-0716-3142-3. This volume explores the latest technologies used in the Polycomb Group of proteins field and helps scientists--working on PcG proteins--investigate all functions of PcG proteins in diverse cellular contexts. The chapters in this book cover topics such the distribution...
2nd edition. — Humana Press, 2023. — 537 p. — (Methods in Molecular Biology 2655). — ISBN 978-1-0716-3143-0. This volume explores the latest technologies used in the Polycomb Group of proteins field and helps scientists--working on PcG proteins--investigate all functions of PcG proteins in diverse cellular contexts. The chapters in this book cover topics such the distribution...
2nd Edition. - Humana Press, 2012. - 381 p. Recent advances in drug discovery have been rapid. The second edition of Bioinformatics and Drug Discovery has been completely updated to include topics that range from new technologies in target identification, genomic analysis, cheminformatics, protein analysis, and network or pathway analysis. Each chapter provides an extended...
3rd Edition. — Humana Press, 2019. — 323 p. Volume 1939 of Methods in Molecular Biology. This third edition volume expands on the previous editions with new topics that cover drug discovery through translational bioinformatics, informatics, clinical research informatics, as well as clinical informatics. The chapters discuss new methods to study target identification, genome...
Humana Press, 2017. — 275 p. — (Methods in Molecular Biology 1503). This volume discusses detailed protocols for the analysis of glycosylation at the level of free glycans and glycopeptides. The book covers topics such as the importance of glycans in eukaryotic life (with a focus on mammals and particularly humans); recent technologies that allow the characterization of larger...
Humana, 2024. — 357 p. This fully updated volume presents a diverse collection of protocols and methodologies for studying various molecular motors. The book explores the dynamics of the bacterial flagellar motor, the mechanisms of ATP synthase, the assembly and function of cytoskeletal motors, and the role of nucleic acid motors in cellular processes, while placing a strong...
Humana Press, 2014. — 589 p. Series: Methods in Molecular Biology, № 1139 The book explores the manipulation and modification of immune cells, the manipulation and modification of tumor cells as well as the manipulation of immune/tumor interactions and various delivery mechanisms, with the overall end goal of evoking a tumor-specific response and overcoming the immuno-evasive...
Humana Press, 2016 — 290p. — (Methods in Molecular Biology 1431) — ISBN: 978-1-4939-3631-1 (eBook), 978-1-4939-3629-8 (Hardcover).
This volume details a valuable collection of protocols and reviews, such as emerging experimental and theoretical approaches. These approaches have resulted in a substantial improvement in the understanding of chromosome architecture. Chromosome...
4th edition. — Humana Press, 2015. — 347 p. — (Methods in Molecular Biology 1334).
This fourth edition reflects the most recent technical advances in DNA-protein interaction research. With fully updated chapters that describe techniques proven by their continuous value, the volume also many new chapters have been added that mostly deal with larger-scale experiments, reflecting...
Humana, 2002. — 266 p. Psychiatric Genetics provides the reader with a complete view of the methodological problems encountered in psychiatry genetics and proposes solutions to commonly occurring questions. The best European and American specialists have given a thorough review on the advantages and disadvantages of genetic epidemiological methods, the way to choose a genetic...
Humana Press, 2017. — 271 p. — (Methods in Molecular Biology 1605). — ISBN10: 1493969862. — ISBN13: 978-1493969869. This volume provides an overview of zygotic genome activation (ZGA) and the use of recent tools to elucidate the events that occur during ZGA. The chapters in this book cover topics such as: clearance of maternal RNAs, detection of miRNA in mammalian oocytes and...
Humana Press, 2022. — 270 р. — (Methods in Molecular Biology 2437). — ISBN 978-1-0716-2029-8. This volume presents updated methods and new developments in the field of mass spectrometry imaging. Chapters guide readers through four parts covering imaging, software, data analysis, new instrumentation, and new methodological approaches. Written in the highly successful Methods in...
Humana Press, 2015 — 2nd Ed. — 414p. — (Methods in Molecular Biology 1315) — ISBN: 978-1-4939-2715-9 (eBook), 978-1-4939-2714-2 (Hardcover).
The primary purpose of this volume is to demonstrate the range of applications of the Pyrosequencing technology in research and diagnostics and to provide detailed protocols. Beginning with an up-to-date overview of the biochemistry, the...
Humana Press, 2007. — 585 p. Series: Methods in Molecular Biology vol.372 Among life scientists, the mitochondrial community originally formed a rather separate and isolated subgroup studying a peculiar organelle. The relevance of mitochondrial research was thought to be limited to the field of bioenergeties. This view changed considerably during the last 10 to 15 years when it...
Humana Press, 2018. - 375 p. - Volume 1673 of Methods in Molecular Biology. This detailed volume provides scientists interested in quorum sensing with a broad spectrum of methods and protocols useful for studying bacterial communication processes at the chemico-physical, molecular, and physiological level. Divided into three sections, the content covers detection and...
Humana Press, 2015 — 255p. — (Methods in Molecular Biology 1331) — ISBN: 978-1-4939-2874-3 (eBook), 978-1-4939-2873-6 (Hardcover). This volume summarizes current cutting-edge methods related to carbohydrate-based vaccines, from the identification of a suitable carbohydrate antigen via the preparation of glycoconjugate vaccines to the characterization of vaccine candidates for...
Humana Press, 2014. — 469 p. Series: Methods in Molecular Biology, № 1092 In this volume, expert researchers in the field detail many of the protocols used to study mouse embryology. These include protocols and techniques that are "close to the embryo": such as, manipulating embryonic gene expression, culturing explanted embryonic tissue and harvesting embryonic RNA. With...
New York: Humana Press, 2015. — 358 p. — (Methods in Molecular Biology 1298). This volume covers the latest technological advances in the characterization of the biosynthesis and functions of Rab GTPases and their regulation by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). The book consists of 28 chapters and starts with an overview of the Rab...
2nd edition. — Humana Press, 2019. — 492 p. — (Neuromethods 146) — ISBN: 978-1-4939-9662-9 This second edition volume expands on the previous edition with updates on the latest techniques used in the field of proteomics. Tissue and cellular proteins are now being analyzed at near proteome-wide levels and mass spectrometers are achieving higher sensitivity and speed, resolution,...
3rd Edition. — 2019. — 191 p. — (Methods in Molecular Biology 2050). — ISBN: 1493997394. This third edition provides in-depth knowledge on the delivery of naked DNA and small-interfering RNA (siRNA) to the targeted microorganism, mammalian single cells, tissues, and animals for prevention and treatment of disease. It builds on the success of the first edition and on the...
New York: Humana, 2021. — 248 p. This detailed book explores the technical breakthroughs with biophysical and cell biological approaches that have advanced the study of molecular mechanisms underlying the generation and transduction of the hedgehog (HH) signal, indicating the important role of sterols in this pathway. Within its pages, the volume examines techniques involving...
Humana Press, 2022. — 258 p. — (Methods in Molecular Biology 2530). — ISBN 978-1-0716-2488-3. This volume provides updated protocols for chemical protein synthesis. Chapters guide readers through development methods, strategies, and applications of protein chemical synthesis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter...
Humana Press, 2022. — 548 p. — (Methods in Molecular Biology 2530). — ISBN 978-1-0716-2489-0. This volume provides updated protocols for chemical protein synthesis. Chapters guide readers through development methods, strategies, and applications of protein chemical synthesis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter...
Humana Press, 2001. — 378 p. Series: Methods in Molecular Biology, № 176 Research into steroid receptor biology holds great promise for the treatment of cancer and endocrine disorders and for elucidating the fundamental biological mechanisms of gene expression. In this volume a distinguished team of principal investigators and their associates describe in step-by-step detail a...
Humana Press, 2004. — 350 p. — (Methods in Molecular Biology 241). In recent years, cell cycle checkpoints, cellular mechanisms that control the cell cycle and ensure genomic stability, have emerged as significant factors in carcinogenesis and in cancer cells. In Cell Cycle Checkpoint Control Protocols, leading investigators present their best methodologies to probe the...
Humana Press, 2005 — 513 p. — eISBN 1-59259-848-X Series: Methods in Molecular Biology, № 292 The application of modern methods in molecular biology and biotechnology to the study of human, animal, and plant viruses continues to revitalize the age-old discipline of virology. Modern virology remains at the vanguard of contemporary biomedical research largely owing to the impact of...
Humana Press, 2021. — 321 p. — (Methods in Molecular Biology 2232). — ISBN: 978-1-0716-1039-8 This volume provides methods, protocols, and reviews that are useful for new and experienced plant microbiome researchers. Chapters guide readers through the investigation of microbiomes associated with seeds, sampling microbiomes from plant compartments and tissues, culture-based...
Humana Press, 2017. — 295 p. — ISBN: 1493969234. Series: Methods in Molecular Biology, № 1592 This volume provides a collection of methodologies for basic research, clinical diagnosis, and treatment pertaining to food allergens, including food allergen production, purification, characterization, detection, quantification, and bioinformatics approaches to modern food allergen...
Humana Press, 2016 — 288p. — (Methods in Molecular Biology 1421) — ISBN: 978-1-4939-3591-8 (eBook), 978-1-4939-3589-5 (Hardcover).
This detailed volume explores the continuing techniques of studying RNA-protein complexes and interactions as research in these areas
expand. After an introductory chapter, the book continues with ways to purify RNA-protein complexes assembled in...
New York: Humana Press, 2012. — 249 p. Today, cells are commonly analyzed en masse, with thousands of cells per sample, yielding results on the average response of the cells. However, cellular heterogeneity implies that in order to learn more about cellular behaviour, it is important to study how individual cells respond, one by one. In Single-Cell Analysis: Methods and...
2nd ed. — Humana Press, 2024. — 201 p. This second edition volume expands on the previous edition with updates on the latest techniques and technologies used in FOXO research. The chapters in this book are organized into five parts. Part One covers biochemical techniques used to analyze phosphorylation status of FOXO proteins, identify FOXO binding partners, and assess...
Humana Press, 2017. — 4394 p. — (Methods in Molecular Biology 1508). This detailed volume presents timely and authoritative content offering a comprehensive overview of the current state of the art in fungal diagnostics. Moreover, it addresses on-going developments expected to provide a basis for targeted treatment strategies resulting in improved outcome of invasive mycoses. The...
Humana Press, 2015 — 271p. — (Methods in Molecular Biology 1319) — ISBN: 978-1-4939-2748-7 (eBook), 978-1-4939-2747-0 (Hardcover). In addition to research and discovery, yeast surface display technology has found applications in industrial processes such as biofuel production and environmental pollutant absorption and degradation. Yeast Surface Display: Methods, Protocols, and...
New York: Humana Press, 2014. — 292 p. — (Methods in Molecular Biology 1084). In this book, expert researchers in the field detail both experimental and computational methods to interrogate molecular level fluctuations. Chapters detail best-practice recipes covering both experimental and computational techniques, reflecting modern protein research. Written in the highly...
New York: Humana Press, 2023. — 253 p. This volume presents a broad collection of state-of-the-art methods to study the biology of thermogenic fat using in vitro cell culture and animal models. Chapters guide the readers through protocols on differentiation of human pluripotent stem cells and murine adipocyte precursors; methods for measuring mitochondrial respiration, heat...
New York: Humana Press, 2022. — 408 p. — ISBN-13 9781071627839. — ISBN-10 107162783X. This second edition compiles state-of-art protocols to study the molecular mechanisms that determine proteome composition and contribute to maintaining protein homeostasis. Chapters describe methods that range from the in vitro analysis of enzyme cascades to real-time in vivo studies. Written...
Humana Press, 1992. - 373 p. ISBN: 0896031993 Series: Methods in Molecular Biology №13 Separation of Large DNA Molecules by Pulsed-Field Gel Electrophoresis. The Isolation of Genomic DNA from Invertebrates. Analysis of Mihondrial DNA Mutations. The Design and Use of Oligonucleotides. The Use of Degenerate Oligonucleotides for Polymerase Chain-Reaction-Based Isolation of Related...
Humana Press Inc., 2002. — 229 p Series: Methods in Molecular Biology, № 184 Dedication Statistical Contributions to Molecular Biology Linking Image Quantitation and Data Analysis Introduction to Microarray Experimentation and Analysis Statistical Methods for Proteomics Statistical Methods for Assessing Biomarkers Power and Sample Size Considerations in Molecular Biology Models...
Humana Press, 2022. — 341 p. — (Methods in Molecular Biology 2517). — ISBN 978-1-0716-2416-6. This volume covers the latest experimental protocols used by researchers to study the recently emerged fungal pathogen of humans, Candida auris, and also explores the pathogen’s antifungal resistance, virulence, and pathogenicity. The chapters in this book cover topics such as the...
New York: Humana Press, 2015. — 411 p. This volume represents a valuable and readily reproducible collection of established and emerging techniques for neuronal cell death research. Conveniently divided into four parts, sections cover a series of techniques for the molecular, structural, functional and genomic characterization of dying neurons, a number of protocols that are of...
3rd edition. — Humana Press, 2023. — 495 p. — (Methods in Molecular Biology 2699). — ISBN13 9781071633618. — ISBN10 1071633619. This third edition expands on the previous editions with updated and new chapters on protein chromatography. Chapters detail protein stability and storage, avoiding proteolysis, protein quantitation methods, generation and purification of recombinant...
Humana Press, 2000. — 529 p. — ISBN-10 0896035808; ISBN-13 978-0896035805. Review. ".does an excellent job of describing the current methodologies in their development, what clinical benefit can be expected, and what is known of their mode of action.has thoroughly covered the current state of the art. " -Antiviral Research. "The present book contains 41 chapters by experts in...
3rd ed. — Humana Press, 2012 — 430 p. — (Methods in Molecular Biology 877) — ISBN: 978-1-61779-818-4. Cell culture methodologies have become standard procedures in most plant laboratories. Currently, facilities for in vitro cell cultures are found in practically every plant biology laboratory, serving different purposes since tissue culture has turned into a basic asset for...
New York: Humana Press, 2015. — 261 p. This volume opens by covering two main types of approaches widely used to determine essential genes: single-gene knockouts and transposon mutagenesis, in both prokaryotes and Candida albicans. Given the significant advancement in the computational predictions of microbial essential genes, the second half of the book examines four main types...
Humana Press, 2021. — 297 p. — (Methods in Molecular Biology 2225). — ISBN: 978-1-0716-1011-4. This detailed volume explores methods and techniques reflecting how viruses can be a beneficial force for all levels of life as well as the mammalian microbiota. Beginning with methods for the development and study of viruses, the book continues with current techniques and approaches...
2nd edition. — Humana Press, 2022 — 351 p. — (Methods in Molecular Biology 2395). — ISBN 978-1-0716-1814-1. This second edition volume expands on the previous edition with an update on the latest techniques used to study plant systems biology on three specific scales: the molecular level, the tissue level, and the whole plant. Chapters cover topics such as gene regulatory...
2nd edition. — Humana Press, 2022 — 766 p. — (Methods in Molecular Biology 2395). — ISBN 978-1-0716-1816-5. This second edition volume expands on the previous edition with an update on the latest techniques used to study plant systems biology on three specific scales: the molecular level, the tissue level, and the whole plant. Chapters cover topics such as gene regulatory...
Humana Press, 2022. — 491 p. — (Methods in Molecular Biology 2536). — ISBN 978-1-0716-2516-3. This volume covers the latest developments in different areas of plant pathology. The chapters in this volume are organized into seven parts. Part One provides traditional methods for isolation and identification of invasive pathogens and root disease. Part Two looks at new and rapid...
Humana Press, 2017. — 272 p. — (Methods in Molecular Biology 1514). This volume provides protocols to successfully apply cutting-edge technologies to characterize the biology of T cells at an unprecedented level of complexity. Chapters guide readers through flow cytometry and fluorescence-activated cell sorting, the behaviour of single T cells after adoptive cell transfer (ACT),...
Humana Press, 2005 — 337 p. — (Methods in Molecular Biology 307). — ISBN: 1-59259-901-X. Study of Cyclic Adenosine Monophosphate Microdomains in Cells High-Resolution Measurements of Cyclic Adenosine Monophosphate Signals in 3D Microdomains Cygnets: In Vivo Characterization of Novel cGMP Indicators and In Vivo Imaging of Intracellular cGMP High-Throughput Screening of...
Humana Press, 2015 — 366p. — (Methods in Molecular Biology 1311) — ISBN: 978-1-4939-2687-9 (eBook), 978-1-4939-2686-2 (Hardcover). This volume presents a list of cutting-edge protocols for the study of CRISPR-Cas defense systems and their applications at the genomic, genetic, biochemical and structural levels. CRISPR: Methods and Protocols guides readers through techniques that...
Humana Press, 2022. — 365 p. — (Methods in Molecular Biology 2569). — ISBN 978-1-0716-2690-0. This volume explores the latest techniques used to study environmental microbial evolution, with a focus on methods capable of addressing deep evolution at long timescales. The chapters in this book are organized into three parts. Part One introduces molecular dating approaches and...
Humana Press, 2019. - 356 p. - Volume 1961 of Methods in Molecular Biology. This detailed volume guides readers through strategic planning and user-friendly guidelines in order to select the most suitable CRISPR-Cas system and target sites with high activity and specificity. Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantification (indel...
Humana Press, 2016 — 3rd Ed. — 191p. — (Methods in Molecular Biology 1392) — ISBN: 978-1-4939-3360-0 (eBook), 978-1-4939-3358-7 (Hardcover).
This updated volume explores a wide variety of clinical applications of PCR such as detecting DNA methylation, detection of viruses and protozoa in infectious diseases, estimation of gene copy number aberrations, primer extension coupled...
2nd Edition. — Humana Press, 2019. — 242 p. — (Methods in Molecular Biology 2020). This second edition volume expands on the previous edition with new insights into the molecular mechanisms involved in allergy through the use of various protocols that cover disciplines like allergy, immunology, cell biology, and histology. The chapters in this book cover topics such as...
Humana Press, 2013. — 311 p. Series: Methods in Molecular Biology, № 953 The study of plant mineral nutrition has both academic and applied aspects to it. Today, research into plant mineral nutrition is more pertinent than ever in the face of a growing world population and the increasing need for sustainable agriculture. In this volume, expert researchers in the field detail a...
New York: Humana Press, 2016. — 299 p. This volume encompasses all major methodologies to interrogate endocannabinoid systems (ECS) and endocannabinoids (eCBs) signaling. With increasing interest towards the manifold activities of eCBs, this book discusses the chemical, biochemical, and molecular biological assays, and activity of distinct elements of the ECS. These include...
2nd edition. — Humana Press, 2023. — 502 p. — (Methods in Molecular Biology 2576). — ISBN 978-1-0716-2727-3. This second edition provides new and updated chapters detailing all major elements of the ECB system. Chapters guide readers through identification of drug targets, electrophysiology, computational chemistry, and machine learning. Written in the successful Methods in...
Humana Press, 2016 — 2nd Ed. — 296p. — (Methods in Molecular Biology 1385) — ISBN: 978-1-4939-3289-4 (eBook), 978-1-4939-3288-7 (Hardcover).
This volume provides up-to-date scientific achievements from the world’s top researchers. Recombinant Proteins from Plants: Methods and Protocols, Second Edition guides readers through protocolsfor use with a variety of plant expression...
Humana Press, 2001. — 335 р. (Methods in Molecular Biology 177). The yeast two-hybrid system is one of the most widely used and productive techniques available for investigating the macromolecular interactions that affect virtually all biological processes. In Two-Hybrid Systems: Methods and Protocols, Paul N. MacDonald has assembled a collection of these powerful molecular tools...
Humana Press, 2012. - 404 p. ISBN: 1617799793 Progenitor cells have become important in regenerative medicine therapies, due to their potential to differentiate into many cell types. This capability, and understanding how to regulate the cells, will provide the basis for future cell therapies aimed at correcting tissue and organ dysfunction as a result of disease or injury. In,...
Springer Science+Business Media, 2021. — 360 p. — (Methods in Molecular Biology 2234). Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts This detailed book collects standard techniques and cutting-edge methods that are frequently used by the research community studying the fungus...
Humana Press, 2000. — 557 p. — (Methods in Molecular Biology 126). In this volume Curtis Machida and a panel of expert investigators present a comprehensive collection of modern molecular methods for analyzing adrenergic receptors and corresponding second messenger systems. These proven and readily reproducible techniques utilize genetic, RNA, protein expression, transactivator,...
Humana Press, 2017. — 546 p. — (Methods in Molecular Biology). — ISBN10: 1493967606. — ISBN13: 978-1493967605. This volume covers an array of techniques available for studying SH2 domains and phosphotyrosine signaling. The book is divided into six parts: Part I outlines the history of SH2, technology development, and cell signaling; Part II focuses on computational approaches...
Humana Press, 2022. — 358 p. — (Methods in Molecular Biology 2397). — ISBN 978-1-0716-1825-7. This book provides comprehensive methods and protocols about enzyme design. The chapters are grouped by main topic, starting with methodologies describing library preparation and screening, state of the art techniques in directed evolution and rational design, followed by examples of...
Humana, 2022. — 483 p. This volume provides computational methods and reviews various aspects of computational studies of protein aggregation. Chapters discuss the relationship between protein misfolding and protein aggregation, methods of prediction of aggregation propensities of protein, peptides, protein structure, results of computer simulations of aggregation, and...
Humana Press, 2020. — 891 p. — (Methods in Molecular Biology 2101). — ISBN 978-1-0716-0219-5. This volume details comprehensive state-of-the-art methods on actin microfilaments and microtubules and how they work to achieve different cellular functions in different cellular contexts. Chapters guide readers through protein purification, in vitro reconstitution of several...
Humana Press, 2015. — 285 p. — (Methods in Molecular Biology 1282). — ISBN: 978-1-4939-2438-7 (eBook), 978-1-4939-2437-0 (Hardcover). This volume aims to describe a variety of techniques that reflects the wide range of research currently performed in the field of coronavirology, and begins with an overview of current understandings of coronavirus replication and pathogenesis to...
Humana, 2025. — 262 p. B-lymphocytes produce antibodies, mediate humoral responses, and act as a key to the adaptive immune system of our body. The development and function of B cells are largely dependent on B cell antigen receptors (BCRs), which transmit a multitude of signals upon binding to the cognate antigen and activate the survival, proliferation, and differentiation of...
Humana Press, 2007. — 496 p. — (Methods in Molecular Biology 393). — ISBN: 1-58829-993-7. Plant Secondary Metabolites provides reliable assays that will equip researchers to meet the unprecedented challenge of fulfilling the huge demand for feed driven by increasing demand of animal protein in developing countries. Using more than a decade of experience working with the...
Humana Press, 2013. — 311 p. Series: Methods in Molecular Biology, № 1054 In this volume expert researchers in the field detail many of the methods which are now commonly used to study DNA using electrophoresis as the major approach. A powerful tool that allows separating DNA molecules according to their size and shape, this volume includes methods and techniques such as...
Humana Press, 2013. — 430 p. — (Methods in Molecular Biology 1049). — ISBN: 162703546X. Ovarian cancer is the most lethal malignancy of the female reproductive system and is also the fifth leading cause of cancer death in women. Sharing common characteristics of cancer, ovarian malignancy possess several clinical and biological particularities In Ovarian Cancer: Methods and...
Humana Press, 2019. — 279 p. — (Methods in Molecular Biology 1881). — ISBN 978-1-4939-8875-4. This book aims to provide scientists with tools and well-researched protocols to enable their research and to facilitate further progress in this common leukemia. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their...
Humana Press, 2014. — 469 p. Series: Methods in Molecular Biology, № 1132 In this volume, expert researchers in the field detail many of the methods which are now commonly used in chloroplast molecular biology. Chapters focus on essential background information, applications in tobacco and protocols for plastid transformation in crops and Chlamydomonas and Bryophytes. Written in...
2nd edition. — Humana Press, 2016. — 565 p. — (Methods in Molecular Biology 2317). — ISBN 978-1-0716-1471-6. This second edition volume expands on the previous edition with updated chapters in model systems and new crops. The book contains protocols for plastid engineering in leaves, tissue culture cells, and the shoot apex of plants, as well as for marker excision from the...
New York: Humana Press, 2015. — 269 p. This volume covers microbiological, clinical and patophysiological aspects of sepsis and also provides general overview chapters with every chapter discussing the real clinical impact of the discussed diagnostic approaches. Sepsis is a major clinical problem that takes an inordinate toll on human lives and economical resources. It is widely...
Humana, 2024. — 331 p. This volume details the development of updated dry lab and wet lab based methods for the reconstruction of Gene regulatory networks (GRN). Chapters guide readers through culprit genes, in-silico drug discovery techniques, genome-wide ChIP-X data, high-Throughput Transcriptomic Data Exome Sequencing, Next-Generation Sequencing, Fuorescence Spectroscopy,...
Humana Press, 2016. — 509 p. — (Methods in Molecular Biology 1382). — ISBN: 978-1-4939-3271-9. This volume provides a clear and detailed roadmap of how to design and execute a gene therapy experiment in order to obtain consistent results. Chapters in this book disseminate bits of unknown information that are important to consider during the course of experimentation and will...
Humana Press, 2019. - 328 p. - Volume 1937 of Methods in Molecular Biology. This volume discusses protocols, ranging from vector production to delivery methods, used to execute gene therapy applications. Chapters are divided into four parts, and cover topics such as design, construction, and application of transcription activation-like effectors; multi-modal production of...
MMB vol.010, Humana Press, 1992, 474 р. ISBN: 0896032043 Production of PolyclonaI Antisera Efficient Elution of Purified Proteins from Polyvinylidene Difluoride Membranes (Immobilon) After Transfer from SDS-PAGE and Their Use as Immunogens Raising Polyclonal Antibodies Using Nitrocellulose-Bound Antigen Synthesis of Pep tides for Use as Immunogens Production and...
Humana Press, 2018. — 375 p. — (Methods in Molecular Biology 1729). — ISBN 978-1-4939-7576-1. This volume covers a wide range of up-to-date technologies that have been successfully applied to study the chemosensing behavior of the traditional model species, such as Escherichia coli and Salmonella typhimurium, while being also applicable to a wide spectrum of other species....
Humana Press, 2020. — 241 p. — (Methods in Molecular Biology 2092). — ISBN 978-1-0716-0174-7. This volume details commonly used molecular and cellular techniques and specialized methodologies for studying retina neuronal subtypes and electrophysiology. Chapters describe techniques for anatomical studies of retinal ganglion cell morphology, gap-junction-mediated neuronal...
Humana Press, 2014. — 327 p. — ISBN: 9781493905621
Series: Methods in Molecular Biology, № 1152
Yeast Metabolic Engineering: Methods and Protocols provides the widely established basic tools used in yeast metabolic engineering, while describing in deeper detail novel and innovative methods that have valuable potential to improve metabolic engineering strategies in industrial...
Humana Press, 2015 — 358p. — (Methods in Molecular Biology 1244) — ISBN: 978-1-4939-1878-2 (eBook), 978-1-4939-1877-5 (Hardcover).
This volume provides complete coverage of the computational approaches currently used in Synthetic Biology. Chapters focus on computational methods and algorithms for the design of bio-components, insight on CAD programs, analysis techniques, and...
2nd edition. — Humana Press, 2021. — 484 p. — (Methods in Molecular Biology 2228). — ISBN 978-1-0716-1023-7. This second edition provides new and updated methods on the principles underlying modern protein analysis, from statistical issues to gel-based and mass spectrometry-based applications. Chapters detail protein quantification as basis for realisation of quantitative...
2nd edition. — Humana Press, 2021. — 484 p. — (Methods in Molecular Biology 2228). — ISBN 978-1-0716-1023-7. This second edition provides new and updated methods on the principles underlying modern protein analysis, from statistical issues to gel-based and mass spectrometry-based applications. Chapters detail protein quantification as basis for realisation of quantitative...
Humana Press, 2016 — 331p. — (Methods in Molecular Biology 1384) — ISBN: 978-1-4939-3255-9 (eBook), 978-1-4939-3254-2 (Hardcover). Exploring the 2-D gel mapping field, the chapters in this book are separated into four different categories: Part I talks about 2-D maps reproducibility and maps modeling; Part II describes the image analysis tools that provide spot volume datasets;...
New York: Humana Press, 2023. — 337 p. This detailed volume provides a comprehensive resource covering the existing and state-of-the-art tools in the field of profiling chromatin accessibility and its dynamics. Beginning with a section on bulk-cell methods for profiling chromatin accessibility and nucleosome positioning that rely on enzymatic cleavage of accessible DNA and...
Humana Press, 2022. — 179 p. — (Methods in Molecular Biology 2514). — ISBN 978-1-0716-2402-9. This volume provides detailed protocols for the identification and understanding of vasculogenic mimicry process in vitro and in vivo, in addition to protocols for microscopy and histology. Chapters guide readers through different materials, commercial and homemade scaffolds, Matrigel,...
Humana Press, 2022. — 408 p. — (Methods in Molecular Biology 2514). — ISBN 978-1-0716-2403-6. This volume provides detailed protocols for the identification and understanding of vasculogenic mimicry process in vitro and in vivo, in addition to protocols for microscopy and histology. Chapters guide readers through different materials, commercial and homemade scaffolds, Matrigel,...
Humana Press, 2017. — 357 p. — (Methods in Molecular Biology 1501). This volume provides key molecular biological protocols and experimental strategies currently employed to study the various stages of mammary gland development. Chapters are divided into four parts covering genetically modified mouse models that exhibit prenatal and pubertal mammary gland phenotypes, 2D and...
Humana Press, 2016 — 321p. — (Methods in Molecular Biology 1399) — ISBN: 978-1-4939-3369-3 (eBook), 978-1-4939-3367-9 (Hardcover). This volume seeks to understand how organisms and gene functions are influenced by environmental cues while accounting for variation that takes place within and among environmental populations and communities. Microbial Environmental Genomics (MEG)...
Humana Press, 2016 — 341p. — (Methods in Molecular Biology 1387) — ISBN: 978-1-4939-3292-4 (eBook), 978-1-4939-3291-7 (Hardcover). This volume describes the most common laboratory procedures for isolation, identification and characterization of polioviruses used in clinical and research laboratories. Written for the Methods in Molecular Biology series, chapters include...
2nd edition. — Humana Press, 2014. — 400 p. — (Methods in Molecular Biology 1183). — ISBN: 978-1-4939-1095-3. Since its inception, patch-clamp has continued to be widely considered the gold standard method to record ion channel activity. Patch-Clamp Methods and Protocols, Second Edition, provides a comprehensive collection of new techniques for the development of automated,...
Springer, 2014. - 507 p. - Volume 1156 of Methods in Molecular Biology. - Shotgun Proteomics: Methods and Protocols serves as a vital collection of protocols through which thousands of proteins can be simultaneously identified, quantified and characterized in a high throughput manner. Beginning with the history of proteomics centered on the vital role of mass spectrometry in its...
Humana Press, 2011. - 307 p. - Methods in Molecular Biology, Volume 778. - The last fifteen years have witnessed the birth and maturation of many original methods and the development of protocols specific to single molecule measurements and their analysis, including techniques involving optical imaging, electron microscopy, optical and magnetic trapping, and developments in...
Humana, 2024. — 573 p. This detailed book delves into the diverse techniques and applications to target, isolate, image, phenotype, and analyze tissue-resident and monocyte-derived macrophages. The contents aim to describe the current knowledge about macrophage development and function which forces the scientific field to move beyond the previously described M1/M2 macrophage...
Humana Press, 2016. — 431 p. — (Methods in Molecular Biology 1418). — ISBN: 1493935763, 9781493935765 EISBN: 9781493935789 This volume expands on statistical analysis of genomic data by discussing cross-cutting groundwork material, public data repositories, common applications, and representative tools for operating on genomic data. Statistical Genomics: Methods and Protocols is...
Humana, 2025. — 292 p. — ISBN 978-1071644331 This volume explores a collection of methodologies used to study the complexities of biological membranes and the associated surfaceome. The chapters in this book aim to facilitate the adoption of innovative methods to help researchers advance their understanding of the biology of membranes and their role in health and disease....
New York: Humana Press, 2016. — 234 p. Series: Methods in Molecular Biology. ISBN: 978-1-4939-3039-5 This third edition of Mitochondrial DNA : Methods and Protocols describes these new methodologies for mitochondrial DNA analysis and, combined with well-established protocols that are considered the gold standard in the fi eld, comprises a compendium of detailed methods for the...
2nd Edition. — Humana Press, 2013. — 417 p. Series: Methods in Molecular Biology, № 1007 Since the publishing of the first edition, the methodologies and instrumentation involved in the field of mass spectrometry-based proteomics has improved considerably. Fully revised and expanded, Mass Spectrometry Data Analysis in Proteomics, Second Edition presents expert chapters on...
Humana Press, 2016. — 505 p. — ISBN: 1493937545 Series: Methods in Molecular Biology, №1449 This volume highlights the role of proteostasis in human health and associated disease model systems, reflecting its rising importance which has led to the development of new technologies to obtain insight into underling protein mechanistic events. Written in the highly successful...
Humana Press, 2009. — 400 p. — ISBN10: 1603271937 Series: Methods in Molecular Biology, №593 Integrated bioinformatics solutions have become increasingly valuable in past years, as technological advances have allowed researchers to consider the potential of omics for clinical diagnosis, prognosis, and therapeutic purposes, and as the costs of such techniques have begun to...
Humana Press, 2009. — 400 p. — ISBN10: 1603271937 Series: Methods in Molecular Biology, №593 This volume discusses the latest developments in clinical proteomics and describes in detail the algorithms used in publicly available software tools. It should be considered as a proteomics-bioinformatics resource and offers the opportunity to understand the details of the various...
Humana Press, 2018. - 466 p. - Volume 1824 of Methods in Molecular Biology. This volume covers several aspects of rational drug design, such as synthesis of novel bioactive drugs; development and application of new methodologies; computational methods valuable for the establishment of new approaches in drug discovery; and the effects of physical-chemical and ADMET properties of...
Springer, 2021. — 344 p. — (Methods in Molecular Biology 2207). — ISBN: 978-1-0716-0919-4. This detailed book aims to provide readers with critical information to accomplish the synthesis of nanosystems for the purpose of supramolecular entities complexing with drugs, targeted drug delivery system characterization, as well as the study of the physical-chemical interactions that...
Humana Press, 2015. — 668 p. — (Methods in Molecular Biology 1280). — ISBN: 978-1-4939-2422-6 (eBook), 978-1-4939-2421-9 (Hardcover). Focusing on essential insight into the methods and techniques required to dissect the complex mechanisms of NF-κB activation, regulation, and function. NF-kappaB: Methods and Protocols guides readers through standard approaches to detect NF-κB...
Humana Press, 2022. — 226 p. — (Methods in Molecular Biology 2435). — ISBN 978-1-0716-2013-7. This volume provides methods and techniques to further the study of cancer immunoprevention. Chapters describe tumor-associated antigens, cancer immune-preventive vaccines, generation of TILs, development of monoclonal antibodies, immunoprofiling technologies, tissue multispectral...
Humana Press, 2022. — 490 p. — (Methods in Molecular Biology 2435). — ISBN 978-1-0716-2014-4. This volume provides methods and techniques to further the study of cancer immunoprevention. Chapters describe tumor-associated antigens, cancer immune-preventive vaccines, generation of TILs, development of monoclonal antibodies, immunoprofiling technologies, tissue multispectral...
Humana Press, 2013. — 252 p. Series: Methods in Molecular Biology, № 1004 Cell death is an essential process in development, and a major contributor to a wide range of human diseases. Three major classifications of cell death, apoptosis, autophagic cell death and necrosis, have been described for years, and the existence of many more forms of cell death is now accepted. In this...
Humana Press, 2016 — 2nd Ed. — 455p. — (Methods in Molecular Biology 1390) — ISBN: 978-1-4939-3335-8 (eBook), 978-1-4939-3333-4 (Hardcover). This second edition volume expands on the first edition with new developments on Toll-Like Receptors (TLRs) controlling events such as cross-priming of associated pattern recognition receptors, post-transcriptional regulation, interaction...
Humana Press, 2019. — 261 p. — (Methods in Molecular Biology, № 2039). — ISBN10: 1493996770, 13 978-1493996773. This volume exploresexperimental and computational approaches to measuring the most widely studied protein assemblies, including condensed liquid phases, aggregates, and crystals. The chapters in this book are organized into three parts: Part One looks at the...
Humana Press, 2019. — 261 p. — (Methods in Molecular Biology, № 2039). — ISBN10: 1493996770, 13 978-1493996773. This volume exploresexperimental and computational approaches to measuring the most widely studied protein assemblies, including condensed liquid phases, aggregates, and crystals. The chapters in this book are organized into three parts: Part One looks at the...
2nd Edition. - Humana Press, 2018. - 250 p. - Volume 1682 of Methods in Molecular Biology. This second edition volume expands on the first edition by providing up-to-date protocols to characterize nanomaterials used as drug delivery agents. The chapters in this book are divided into 5 parts and cover topics such as: advances and obstacles in nanomedicine research; methods to test...
Humana Press, 2023. — 201 p. — (Methods in Molecular Biology 2708). — ISBN 978-1-0716-3408-0. This collection provides methods and techniques employed when culturing, manipulating, quantifying, and functionally assessing retinal ganglion cells (RGCs), vital for better understanding various ocular diseases and for developing novel therapies. The book features exhaustive and...
Humana Press, 2020. — 411 p. — (Methods in Molecular Biology 2052). — ISBN 978-1-4939-9747-3. This book encompasses broad aspects of Cryptosporidium research with established methods that have been improved and expanded over the years as well as recent cutting-edge techniques. Within this collection are numerous molecular methods as well as protocols for genotyping and...
2nd ed. — Humana Press, 2024. — 224 p. This fully updated volume serves as multidisciplinary compendium of approaches and techniques employed to analyze the role of different molecules, processes, or strategies used by different guests to survive and proliferate in their associations with eukaryotic hosts. Beginning with animal-pathogen interactions, the book then continues...
Humana, 2024. — 271 p. Dedication Preface Contributors Introduction Host-Pathogen Interaction: Biology and Public Health Genetic Association Studies in Host-Pathogen Interaction Analysis Viral-Host Interactions Live-Cell Fluorescence Imaging for Virus-Host Interactions Construction of a Mycoviral Infectious Clone for Reverse Genetics in Botrytis cinerea Plant-Pathogens...
Second Edition, Humana Press, 2012. - 614 p. ISBN: 1627030255 Over the past ten years, many powerful new techniques have been developed that have dramatically changed malaria research. The second edition of Malaria: Methods and Protocols expands upon the previous edition with current, detailed techniques for laboratory research. With new chapters on parasite culture techniques,...
Humana Press, 2008. — 291 p. The field of toxicogenomics has been growing quickly, yet with rapid growth comes the need to periodically reflect on the nature of the field. Essential Concepts in Toxicogenomics collects reviews, opinion pieces and case studies from the leading experts in this dynamic field with a focus on its application to pharmaceuticals. Topics covered include...
Springer Science+Business Media, LLC, 2018. — 297 p. — (Methods in Molecular Biology 1747). — ISBN: 149397694X. This volume explores three main aspects of nitric oxide (NO) research: NO treatment and detection, NO modifications, and NO detoxification. The book also covers methods used to study human/animal and plant nitric oxide. The chapters are divided into three parts: part...
Springer Science+Business Media, LLC, 2018. — 297 p. — (Methods in Molecular Biology 1747). — ISBN: 149397694X. This volume explores three main aspects of nitric oxide (NO) research: NO treatment and detection, NO modifications, and NO detoxification. The book also covers methods used to study human/animal and plant nitric oxide. The chapters are divided into three parts: part...
2nd edition. — Humana Press, 2021. — 268 p. — (Methods in Molecular Biology). — ISBN 978-1-0716-1098-5. This completely revised edition explores novel discoveries in bacterial genomic research, with a focus on technical and computational improvements as well as methods used for bacterial pangenome analysis, which relies on microbiome studies and metagenomic data. Beginning with...
2nd edition. — Humana Press, 2021. — 268 p. — (Methods in Molecular Biology). — ISBN 978-1-0716-1098-5. This completely revised edition explores novel discoveries in bacterial genomic research, with a focus on technical and computational improvements as well as methods used for bacterial pangenome analysis, which relies on microbiome studies and metagenomic data. Beginning with...
New York: Humana Press, 2015. — 309 p. Bacterial genomics is a mature research interdisciplinary field, which is approached by ecologists, geneticists, bacteriologists, molecular biologists and evolutionary biologists working in medical, industrial and basic science. Thanks to the large diffusion of bacterial genome analysis, Bacterial Pangenomics: Methods and Protocols is able to...
Springer, 2021. — 356 p. — (Methods in Molecular Biology 2229). — ISBN 978-1-0716-1031-2. This volume provides clear and direct protocols to implement automated Design-Build-Test-Learn (DBTL) into synthetic biology research. Chapters detail techniques to model and simulate biological systems, redesign biological systems, setting up of an automated biolaboratory, step-by-step...
Humana Press, 2023. — 240 p. — (Methods in Molecular Biology 2706). — ISBN13 9781071633960. — ISBN10 1071633961. This volume presents both theoretical guidance and protocols on chemogenomics including chemogenomics library assembly, compound profiling, and phenotypic assays. The chapters in this book cover topics such as the assembly and use of Kinase Chemogenomics; data mining...
Humana Press, 2003. — 255 p. — (Methods in Molecular Biology 219). Cellular and gene-based experimental techniques promise powerful new therapeutic agents for the remediation of both acquired and inherited cardiovascular disease. In Cardiac Cell and Gene Transfer: Principles, Protocols, and Applications, Joseph M. Metzger and a panel of leading scientific experts detail many...
Humana Press, 2022. — 298 p. — (Methods in Molecular Biology 2529). This detailed volume explores techniques to study reactive oxygen species (ROS) in plants and to characterize their roles in development and stress responses. Beginning with a section on strategies to induce ROS production, the book continues with methods to visualize ROS and detect changes in redox...
Humana Press, 2016 — 244p. — (Methods in Molecular Biology 1333) — ISBN: 978-1-4939-2854-5 (eBook), 978-1-4939-2853-8 (Hardcover). This volume presents a comprehensive collection of methods that have been instrumental to the current understanding of bacterial persisters. Chapters in the book cover topics ranging from general methods for measuring persister levels in Escherichia...
Humana Press, 1998. — 344 p. — (Methods in Molecular Biology 104). In this volume Roger Miles and Robin Nicholas present a collection of cutting-edge methods for the detection, isolation, identification, characterization, and genetic manipulation of the pathogenic mycoplasmas. These step-by-step methods are crafted for successful reproducibility and include biochemical,...
Humana Press, 2014. — 441 p. — (Methods in Molecular Biology 1135). Providing a selection of the key techniques that are used in characterizing cerebral angiogenesis, the book aims to define the cellular and molecular mechanisms underlying this important process. Divided into six parts, this detailed volume examines cerebral angiogenesis occurring in different scenarios, a variety...
Humana Press, 2010. - 444 p. - Methods in Molecular Biology, Volume 623. - From the early days when RNA interference was a strange artifact in worms to the 2006 Noble Prize received by Fire and Mello and the current clinical trials, the field of RNA interference has grown at a breakneck pace. In RNA Interference: From Biology to Clinical Applications, expert contributors...
Humana Press, 2017. — 318 p. — (Methods in Molecular Biology 1593). — ISBN10: 1493969269. — ISBN13: 978-1493969265. This volume examines the structure and dynamics of the bacterial flagellum using bacterial genetics, molecular biology, biochemistry, structural biology, biophysics, cell biology, and molecular dynamics simulation. The chapters are divided into 4 parts: Part I...
Humana Press, 2017. — 273 p. — (Methods in Molecular Biology). — ISBN10: 1493966537. — ISBN13: 978-1493966530. This volume explores data from the applications of molecular biological methods and the applications of recent immunological and cytogenetic methods in Epstein-Barr Virus (EBV) that will offer readers possible new solutions to the unresolved problems in the EBV field....
2nd Edition. - Humana Press, 2017. - 231 p. Volume 1504 of Methods in Molecular Biology. This volume introduces the reader to the field of enzyme stabilization and the different theories of enzyme stabilization, including the use of immobilization as a stabilization technique. The first part of the book focuses on protocols for enzyme stabilization in solutions including...
Humana, 2021. — 467 p. This second edition provides new and updated chapters useful for the study of Regulatory B cells. Organized in four sections, chapters detail basic methods for the isolation and immunophenotypical analysis of these cells, experimental approaches for the ex vivo generation/expansion of IL-10 producing B cells, molecular biology techniques for the analysis...
Humana Press, 2023. — 443 p. — (Methods in Molecular Biology 2649). — ISBN 978-1-0716-3071-6. This volume describes different sequencing methods, pipelines and tools for metagenome data analyses. Chapters guide readers through quality control of raw sequence data, metagenomics databases for bacterial annotations such Greengenes, SILVA, RDP and GTDB, guide to 16S rRNA microbiome...
3rd ed. — Humana Press, 2012. — 426 p. — ISBN: 978-1-61779-367-7 Series: Methods in Molecular Biology, № 806 Preface Contributors Introduction to Cell Culture Isolation and Cultivation of Dermal Stem Cells that Differentiate into Functional Epidermal Melanocytes Isolation, Cultivation, and Application of Human Alveolar Epithelial Cells Culture of Parathyroid Cells Peyman...
Springer, 2021. — 286 p. — (Methods in Molecular Biology 2258). — ISBN-13. 978-1-0716-1174-6 This detailed book explores techniques for understanding and engineering programs that naturally control and drive formation of tissues and organs in order to open powerful opportunities to produce physiologically relevant tissues of interest, generate models to study human disease, and...
Humana Press, 2022. — 294 p. — (Methods in Molecular Biology 2409). — ISBN 978-1-0716-1878-3. This detailed volume explores various aspects of the dengue virus and its four serotypes (DENV1-4). Beginning with a section on protocols to produce DENV and its proteins that are fundamental for many biophysical, biochemical, immunological, and cellular studies, the book continues by...
Humana Press, 2022. — 640 p. — (Methods in Molecular Biology 2409). — ISBN 978-1-0716-1879-0. This detailed volume explores various aspects of the dengue virus and its four serotypes (DENV1-4). Beginning with a section on protocols to produce DENV and its proteins that are fundamental for many biophysical, biochemical, immunological, and cellular studies, the book continues by...
Humana, 2025. — 274 p. — ISBN-13 : 978-1071644171 This third edition book explores breakthrough techniques in the field of drug target identification and validation. With technologies and methodologies ranging from target identification to the analysis of clinical samples, as well as newer areas like artificial intelligence and machine learning applications, the book will aid...
Humana Press, 2013. — 389 p. Series: Methods in Molecular Biology, № 986 The major reason for the elevated costs of drug development in the pharmaceutical industry is the high attrition rate. In this volume expert researchers in the field detail many of the methods which are now commonly used to identify and validate new target. These include methods and approaches covering...
2nd edition. — Humana Press, 2019. — 316 p. — (Methods in Molecular Biology 1953). — ISBN: 978-1-4939-9144-0. This second edition book explores breakthrough technologies in the field of drug target identification and validation. The volume emphasizes particularly revolutionary technologies, such as CRISPR-related screening, “big data,” and in silico approaches, as well as in...
Humana Press, 2013. — 445 p. Series: Methods in Molecular Biology, № 992 The haemostatic system is one the most important physiological systems for maintaining health and well being, and thus the investigation of the haemostatic system remains a research priority. Disturbances of the haemostatic system in the broader sense, such as heart disease and strokes, arguably constitute...
Humana Press, 2017. — 277 p. — (Methods in Molecular Biology 1505). This detailed book collects the main methodologies used for the analysis of the activity, localization, and regulation of the components of the Mitotic Exit Network (MEN) pathway during mitotic exit in Saccharomyces cerevisiae, as well as for the evaluation of the roles of these proteins in other cellular...
Humana Press, 2013. — 697 p. — ISBN: 1627030166. Over the past 40 years the field of molecular simulations has evolved from picosecond studies of isolated macromolecules in vacuum to studies of complex, chemically heterogeneous biological systems consisting of millions of atoms, with the simulation time scales spanning up to milliseconds. In Biomolecular Simulations: Methods...
Humana, 2010. - 322 p. - In recent years, the critical role of microRNAs has been revealed within the biology of cells that constitute the immune system. In MicroRNAs and the Immune System: Methods and Protocols, expert researchers explore the latest techniques for studying miRNA expression, including the most up-to-date data on splinted ligation and qRT-PCR assays, as well as...
New York: Humana Press, 2015. — 361 p. This volume presents a valuable and readily reproducible collection of established and emerging techniques on modern genetic analyses. Chapters focus on statistical or data mining analyses, genetic architecture, the burden of multiple testing, genetic variance, measuring epistasis, multifactor dimensionality reduction, and ReliefF. Written in...
2nd edition. — Humana Press, New York, 2015. — 208 p. — ISBN: 1493916602, 9781493916603 Series: Methods in Molecular Biology, N1219 Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts In Apoptosis and Cancer: Methods and Protocols, Second Edition, expert...
Humana Press, 2017. — 373 p. — (Methods in Molecular Biology 1542). This thorough volume explores the possibility of detecting and identifying toxigenic fungi, able to produce secondary metabolites known as mycotoxins, which cause severe health problems in humans and animals after exposure to contaminated food and feed, having a broad range of toxic effects, including...
3rd Edition. - Humana Press, 2009. - 674 p. - Gene expression can mean the difference between a functional and non-functional genome, between health and disease, and with the development of transgenic crops, the difference between survival and starvation. In DNA-Protein Interactions: Principles and Protocols, Third Edition, this vital subject is brought up to date with...
New York: Humana Press, 2023. — 323 p. This volume details protocols on animal cloning by Somatic cell nuclear transfer for basic research and biotechnological applications. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on...
Humana Press, 2001. — 331 p. Series: Methods in Molecular Biology, № 62 The use of molecular technologies to study Parkinson's disease in the laboratory is beginning to have a major impact in clinical medicine. In this volume internationally recognized biomedical investigators describe in detail the major techniques employed in molecular and cellular studies of Parkinson's...
Humana Press, 2023. — 290 p. — (Methods in Molecular Biology 2645). — ISBN 978-1-0716-3055-6. This volume explores the latest collection of cell models that are used in preclinical cancer research, and covers both two-dimensional and three-dimensional culturing techniques. The chapters in this book are divided into two parts. Part One discusses two-dimensional cancer cell...
Humana Press, 2018. — xi + 274 p. — ISBN: 978-1-4939-8681-1. The aim of this book is to compile the most comprehensive and up-to-date collection of cutting-edge protocols used in wet and dry lab to investigate the viral communities harbored within and on the human body. A practical and experience-based book, it includes methods for collection, isolation, identification, and...
Humana, 2023. — 480 p. This detailed volume provides a comprehensive collection of classic and cutting-edge methods and techniques in mapping protein-protein interactions. The chapters include a variety of in vitro and in vivo experimental methods covering cell biology, biochemistry, and biophysics. In addition, the book also explores in silico methods including sequence-,...
Humana Press, 2021. — 307 p. — ISBN 978-1-0716-1533-1. This book provides methods and techniques used in construction of global transcriptional regulatory networks in diverse systems, various layers of gene regulation and mathematical as well as computational modeling of transcriptional gene regulation. Written in the highly successful Methods in Molecular Biology series...
Humana Press, 2021. — 307 p. — ISBN 978-1-0716-1533-1. This book provides methods and techniques used in construction of global transcriptional regulatory networks in diverse systems, various layers of gene regulation and mathematical as well as computational modeling of transcriptional gene regulation. Written in the highly successful Methods in Molecular Biology series...
Humana Press, 2022. — 415 p. — (Methods in Molecular Biology 2376). — ISBN 978-1-0716-1715-1. This volume provides comprehensive protocols on experimental and computational methods that are used to study probe protein folding reactions and mechanisms. Chapters divided into five parts detail protein engineering, protein chemistry, experimental approaches to investigate the...
Humana Press, 2016. — 231 p. — (Methods in Molecular Biology 1469). — ISBN 978-1-4939-4929-8. This volume assembles protocols for chromosome engineering and genome editing in two recently developed approaches for manipulating chromosomal and genomic DNA in plants. The first approach is a “plant chromosome vector” system, which allows the introduction of desired genes or DNA...
Humana Press, 2016 — 3rd Ed. — 508p. — (Methods in Molecular Biology 1350) — ISBN: 978-1-4939-3043-2 (eBook), 978-1-4939-3042-5 (Hardcover). The third edition of this volume expands upon the previous two editions with new and up-to-date methods and protocols. Chapters include step-by-step procedures involved in quantifying cell growth, baculovirus infection and cell metabolism,...
MMB vol.018, Humana Press, 1993, ISBN: 0896032450, 436р. Introduction to Transgenesis Transgenic Rodents and the Study of the Central Nervous System Transgenic Animals and the Study of Cancer Transgenic Animals and the Study of the Immune System The Commercial and Agricultural Applications of Animal Transgenesis Commercial and Agricultural Appllcations P Element-Mediated...
Humana Press, 2001. — 252 pp. Series: Methods in Molecular Biology, Volume 168. With the huge increase in available data on the DNA sequences of proteins, there is a growing need to understand and characterize how proteins fold into their biologically active native states and the basis for the stability of these states. In Protein Structure, Stability, and Folding, Kenneth P....
Humana Press, 2014. — 267 p. Series: Methods in Molecular Biology, № 1162 The book brings together a diverse set of techniques for the study of the mechanisms underlying central nervous system axon growth, consequently providing a resource that will aid in the development of repair strategies. After an introductory section, this detailed volume continues with sections focusing on...
MMB vol.007 Humana Press, 1991, ISBN: 0896031780, 416р. Murray's new handbook on Gene Transfer and Expression Protocols sets forth both current and new methodologies in a clear, concise, easy-to-follow manner, following the successful formula of the classic volumes in Humana's Methods in Molecular Biology series. Each chapter is devoted to a thorough exposition of a single...
2nd edition. — Humana Press, 2011. — 512 p. — (Methods in Molecular Biology 755) — ISBN: 978-1-61779-162-8 Laser microdissection techniques have revolutionized the ability of researchers in general, and pathologists in particular, to carry out molecular analysis on specific types of normal and diseased cells and to fully utilize the power of current molecular technologies...
Humana Press, 2018. — 234 p. — (Methods in Molecular Biology 1810). — ISBN 978-1-4939-8578-4. This volume features a comprehensive set of protocols featuring a range of both old and new technologies that can be used to analyze drugs of abuse, including prescription drugs, new psychoactive substances and psychoactive plants. Chapters guide readers through the application of...
Humana Press, 2018. - 654 p. - Volume 1672 of Methods in Molecular Biology. This volume presents forty-two methods and protocols to analyze diverse aspects of genome instability. Chapters detail mutagenesis and repair, methods to quantify and analyze the properties of DNA double-strand breaks, profile replication, replication proteins strand-specifically, genome instability,...
New York: Humana Press, 2023. — 342 p. This volume details applications, methods, and protocols on nucleic acid amplification. Chapters detail the management of organ and stem cell transplantation, constitutional genetics and genomics, epidemiology, public health, nucleic acid detection, methods for amplification and sequencing, nuclease-assisted mutant allele enrichment,...
Humana: 2024. — 361 p. This second edition volume expands on the previous edition with updated protocols on basic techniques of radioligand binding, allosteric modulation, visualization and antibody use, and autoradiography and non-neuronal acetylcholine detection. The updated chapters also cover the latest fluorescent techniques including fluorescence anisotropy, and provide...
Humana Press, 2015. — 321 p. Series: Methods in Molecular Biology, № 1287 This volume provides readers with a comprehensive review of various gene silencing methodologies and its applications. It provides a historical overview of gene silencing mechanisms in plants, vectors, and strategies available for plant gene silencing, practical applications of gene silencing, bioinformatics...
2nd edition. — Humana Press, 2021. — 881 p. — (Methods in Molecular Biology, 2342). — ISBN 978-1-0716-1553-9. This second edition further develops the principles of applying kinetic principles to drug metabolizing enzymes and transporters. Chapters are divided into six sections detailing fundamental principles of enzyme kinetics, enzyme and transporter structures, highlighting...
Humana Press, 2010. — 444 p. Given the vital importance of immune system research, the gathering of clear, consistent, and informative protocols involving the study of dendritic cells is paramount. Bringing the popular first edition fully up to date, Dendritic Cell Protocols, Second Edition presents protocols from experts in the field that cover the basics and more complex...
Humana Press, 2015 — 351p. — (Methods in Molecular Biology 1262) — ISBN: 978-1-4939-2253-6 (eBook), 978-1-4939-2252-9 (Hardcover). This volume focuses on cytological, biochemical, and molecular biological methods to identify and examine the function of each nuclear body, with an emphasis on the analysis of long non-coding RNAs. Chapters focus on exploring recent studies that...
Humana Press, 2017. — 205 p. — (Methods in Molecular Biology). — ISBN10: 1493968297, 13 978-1493968299. This volume focuses and describes tools, assays, and techniques used to enhance the understanding of the role of auxins and cytokinins. This volume focuses and describes tools, assays, and techniques used to enhance the understanding of the role of auxins and cytokinins. The...
New York: Humana Press, 2021. — 391 p. Although we have advanced the field of microbial systems biology significantly during the last decade, our foray into analyzing exciting and evermore complex systems such as gut microbiome or autotroph-bacterial interactions requires examining all available data at our disposal. Systems biology analyses today usually use multiple types of...
Springer, 2012. — 611 p. — (Methods in Molecular Biology). — ISBN 978-1-61779-826-9. This second edition provides new and updated chapters describing the utility and attributes of different tools that are used for studying microbial systems. Chapters detail a number of methods, including in silico system level analyses by MetaFlux, Kbase genome-scale model builder, COBRA...
New York: Humana Press, 2015. — 350 p. The book highlights major techniques, both experimental and computational, for the study of tissue morphogenesis, divided into several sections, with specific focus on techniques to image, manipulate, model and analyze tissue morphogenesis. Chapters focus on imagining analysis of tissue morphogenesis, culture models of tissue morphogenesis,...
Humana Press, 2016. — 293 p. — ISBN: 1493937901 Series: Methods in Molecular Biology, № 1455 This volume provides an up-to-date compilation of current methodological approaches utilized for the exploration of nucleolar structure and function. Chapters cover a diversity of protocols that include imaging of the nucleolus, analysis of ribosomal RNA transcription and processing,...
Humana Press, 2022. — 459 p. — (Methods in Molecular Biology 2503). — ISBN 978-1-0716-2333-6. This volume provides a compilation of methods that will aid researchers studying virology, immunology, and vaccinology of this devastating disease of swine. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the...
Humana Press, 2022. — 223 p. — (Methods in Molecular Biology 2503). — ISBN 978-1-0716-2332-9. This volume provides a compilation of methods that will aid researchers studying virology, immunology, and vaccinology of this devastating disease of swine. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the...
Humana Press, 2022. — 264 p. — (Methods in Molecular Biology 2531). — ISBN 978-1-0716-2492-0. This volume details aspects and applications of interfacing capillary electrophoresis (CE) with mass spectrometry (MS). Chapters guide readers through approaches based on different types of CE-MS interfaces such as (nano)sheath liquid, porous tip, and liquid junction, as well as...
3rd edition. — Humana Press, 2018. — 536 p. — (Methods in Molecular Biology 1827). — ISBN 978-1-4939-8647-7. This detailed new edition provides complete and easy access to a variety of antibody engineering techniques. The volume explores topics such as the generation of native, synthetic, or immune antibody libraries, the selection of lead candidates via the different powerful...
New York: Humana Press, 2023. — 387 p. This volume focuses on the computational modeling of cell signaling networks and the application of these models and model-based analysis to systems and personalized medicine. Chapters guide readers through various modeling approaches for signaling networks, new methods and techniques that facilitate model development and analysis, and new...
Humana Press, 2023. — 465 p. — (Methods in Molecular Biology 2615). — ISBN 978-1-0716-2921-5. This volume compiles a comprehensive range of methods to study key aspects of mitochondrial DNA including nucleoid structure and packaging, replication, genome integrity, and disease. Chapters are organized into eight methodological sections that cover in vitro and in vivo methods,...
Humana Press, 2013. — 317 p. Series: Methods in Molecular Biology, № 967 The receptor-associated JAK protein kinases and their substrates, the STAT transcriptional activators, transmit signals following cytokine and growth factor binding to receptors expressed on the cell surface, to result in specific transcriptional and cellular responses. Over the last two decades, the field...
Humana Press, 2022. — 401 p. — (Methods in Molecular Biology 2510). — ISBN 978-1-0716-2383-1. This detailed volume covers diverse aspects of P2X7 receptor analysis, ranging from its molecular structure to related pharmacological and immunological tools, via its analysis in heterologous expression systems as well as assays using primary cells and whole animal models. After three...
Humana Press, 1995. - 358 p. ISBN: 089603304X Series: Methods in Molecular Biology, №48 Contents Theory and Instrumentation. Electroporation Theory: Concepts and Mechanisms. Effects of Pulse Length and Strength on Electroporation Efficiency. Instrumentation. Electroporation Prools. The Introduction of Proteins into Mammalian Cells by Electroporation. Electroporation of...
MMB vol.047 Humana Press, 1995, ISBN: 0896033104, 369р. Electroporation Protocols for Microorganisms is the first complete guide to the electroporation of nearly all microorganisms of importance used in biological and biomedical research. It includes reproducible protocols for diverse bacterial, fungal, and protist species-many of which are important in human disease-as well as...
Humana, 2024. — 441 p. This second eidtion explores new and updated techniques used to understand solid target-specific models in computational toxicology. Chapters are divided into four sections, detailing molecular descriptors, QSAR and read-across, molecular and data modeling techniques, computational toxicology in drug discovery, molecular fingerprints, AI techniques, and...
Humana Press, 2010. — 344 p. — ISBN: 1588299139 Series: Methods in Molecular Biology, №703 Recent insight into the transcripts generated from the mammalian genome (i.e. the transcriptome) has revealed that transcription is a far more complex phenomenon than previously thought. In RNA: Methods and Protocols, expert researchers provide the procedures and methods used to describe...
Second Edition. — Humana Press, 2014. — 245 p. Series: Methods in Molecular Biology, № 1050 Exemplifying and illustrating recent exciting advances in PNA chemistry, the second edition serves as a vital complement to the first edition of the book. Since the discovery of peptide nucleic acids, many interesting new derivatives and analogues in terms of nucleic acid recognition...
Humana Press, 2002. — 259 p. Series: Methods in Molecular Biology, № 208 Peptide nucleic acid (PNA) technology continues to grow in importance throughout molecular biology, genetic diagnostics, and molecular medicine. In Peptide Nucleic Acids: Methods and Protocols, Peter Eigil Nielsen has assembled a critically evaluated collection of PNA protocols that are either already well...
Humana Press, 2004. — 333 p. Series: Methods in Molecular Biology, № 283 Contemporary approaches to the synthesis of chemically modified proteins not only require efficient means to control conjugation and the specific site of attachment of the conjugated moiety, but also the effective use of recent developments in microarray technologies and nanobiotechnology. In...
Humana Press, 2005. — 537 p. — eISBN 1-59259-912-5 Series: Methods in Molecular Biology, № 305 Isothermal Titration Calorimetry Direct Optical Detection of Protein–Ligand Interactions Label-Free Detection of Protein–Ligand Interactions by the Quartz Crystal Microbalance Measurement of Solvent Accessibility at Protein–Protein Interfaces Hydrophobic Interaction Chromatography:...
Humana Press, 2017. — 219 p. — (Methods in Molecular Biology 1534). This book offers in a single volume a unique collection of the state-of the-art experimental procedures utilized for the induction, detection, and modeling of this complex cellular program of oncogene-induced senescence. The book encompasses protocols for studying this multi-step program in human specimens and...
Humana Press, 2017. — 221 p. — (Methods in Molecular Biology 1531). This volume discusses various basic and advanced methods and protocols that have been proven to be successful among certain bacterial species, or a family of species, in type III secretion systems (T3S system). The chapters in this book cover topics such as: site-directed mutagenesis and its application in...
Humana Press, 2018. - 453 p. - Volume 1777 of Methods in Molecular Biology. This volume details methods and protocols on b-sheet assemblies and collagen. Divided into three parts chapters focus on expanding use of solid-state NMR as a powerful method to enhance structural understanding of self-assembled peptide materials, methods for the design, synthesis, and application of...
Humana Press, 2014. — 282 p. Series: Methods in Molecular Biology, № 1088 The book features biological methods for the preparation of peptide phage display libraries using both filamentous and lytic phage. With contributions from renowned authors in the field, the book also explores selection and screening of the prepared peptide libraries for peptides with the desired function...
Humana Press, 2021. — 349 p. — (Methods in Molecular Biology 2445). — ISBN 978-1-0716-2070-0. This volume includes comprehensive methods and protocols on autophagic pathways in cancer disorders. Chapters cover basic principles of autophagy methodology in cancer cells, translational approaches, systematic computational analyses of TCGA (The Cancer Genome Atlas) cohorts, CCLE...
Humana Press, 2023. — 370 p. — (Methods in Molecular Biology 2656). — ISBN 978-1-0716-3138-6. This volume explores the latest techniques used in the field of mammalian germ cell biology to study key experimental approaches and perspectives for critical analysis of spermatogonial subsets including the rare spermatogonial stem cells (SSCs) pool. Written in the highly successful...
Humana Press, 2012. — 237 p. Series: Methods in Molecular Biology, № 826 Increasing evidence suggests that liver stem cells have the capacity to differentiate into parenchymal hepatocytes or into bile ductular cells. These stem cells may be activated to proliferate after severe liver injury or exposure to hepatocarcinogens. Stem cell replacement strategies are being...
Humana Press, 2013. — 219 p. Series: Methods in Molecular Biology, № 968 Invasive fungal infections are a significant cause of morbidity and mortality. Over the past decade there has been a concerted effort to develop reliable methods for the detection of such infections. In this volume, expert researchers in the field detail the introduction of new technology into a diagnostic...
Humana Press, 2014 — 289p. — (Methods in Molecular Biology 1111) — ISBN: 978-1-62703-755-6 (eBook), 978-1-62703-754-9 (Hardcover). Artificial riboswitches and other ligand-responsive gene regulators make it possible to switch protein synthesis ON or OFF with arbitrary ligand molecules. Artificial Riboswitches: Methods and Protocols focuses on the state-of-the-art methods...
Humana Press, 1995. - 312 p. ISBN: 0896032825 Series Methods in Molecular Biology, №43 Alternative Method of Assessing Toxicity. Target Organ Toxicity: LLC-RK1 Cell Screening Test for Nephrotoxicity. Preparation and Use of Cultured Astrocytes for Assay of Gliotoxicity. Human Thyroid Culture. Thyroid Follicular Cells in Monolayer Culture: In Vitro Models for Thyroid Toxicity...
Humana Press, 2018. - 310 p. - Volume 1664 of Methods in Molecular Biology. This volume provides a comprehensive study of this key method of gel-based proteomics. Chapters in Difference Gel Electrophoresis: Methods and Protocols detail methods on principles of differential protein labeling, two-dimensional gel electrophoresis, techniques on optimized proteomic workflows, advanced...
Humana Press, 2018. - 262 p. - Volume 1680 of Methods in Molecular Biology. This volume covers methods that analyze various Argonaute proteins from a variety of organisms to help researchers better understand their properties ranging from a molecular level to an organismal level. The chapters in this book explore the following topics: identification and expression analysis of...
Humana, 2021. — 317 p. This volume highlights various techniques used to study multiple aspects of mGlu receptor function. Chapters in this book cover topics such as modern approaches for the development and screening of allosteric modulators; fluctuations in intracellular calcium induced by mGlu receptor activation; dopamine-glutamate interactions in the central nervous...
New York: Humana Press, 2022. — 276 p. This volume provides methods and models for investigating the immunosuppressive subset of CD4+ T-cells, regulatory T-cells (Treg). Chapters guide readers through, basic protocols for analyzing Treg in mice and humans, single-cell analysis for analyzing Treg, and methods for the analysis of Treg in clinical application. Written in the...
Springer Science+Business Media, New York, 2017. — 358 p. — (Methods in Molecular Biology. Vol.1479) — ISBN: 1493963627 This volume provides a unique forum to review cell microencapsulation in a broad sense by exploring various cell types that have been encapsulated for different purposes, different approaches and devices used for microencapsulation, the biomaterials used in...
Humana Press, 2023. — 273 p. — (Methods in Molecular Biology 2650). This volume details the most up-to-date methods of intestinal differentiation program. Chapters guide readers through techniques to characterize intestinal differentiated cell, RNAseq, single-cell RNAseq transcriptional profiling methods, isolation of intestinal crypts, and methods for studying intestinal...
New York: Humana Press, 2015. — 201 p. Series: Methods in Molecular Biology, № 1252 This volume emphasizes new techniques to help understand protein cages and to apply them to a variety of technologies, highlighting the expertise of researchers based on three continents. Protein cages are currently inspiring diverse scientific disciplines and are therefore at the crossroads of...
Humana Press, 2017. — 265 p. — (Methods in Molecular Biology 1468). — ISBN 978-1-4939-4033-2. This volume provides a comprehensive overview of the experimental and computational methodologies used to study the function of long non-coding RNA (ncRNAs) expressed from enhancers. Chapter detail both wet-lab and dry-lab techniques and annotating long ncRNAs and exploring...
Humana Press, 2015 — 227p. — (Methods in Molecular Biology 1336) — ISBN: 978-1-4939-2926-9 (eBook), 978-1-4939-2925-2 (Hardcover). This volume contains a collection of relevant information for drug discovery in cell cycle research. Protocols to develop screening assays or to identify novel CDK inhibitors are discussed in the first part of the book. The second part of the book...
New York: Humana Press, 2015. — 259 p. — (Methods in Molecular Biology 1292). This volume focuses on detecting different cellular stresses, measuring pathological consequences within the cell, and investigating the role of cellular stresses in select diseases. In addition, this book reviews the crosstalk between different stress pathways, stress responses during ageing, and...
Humana Press, 2022. — 923 p. — (Methods in Molecular Biology 2393). — ISBN 978-1-0716-1802-8. This volume provides detailed technical protocols on current biosensors and imaging technologies and Chapters focus on optical, electrochemical, Quartz crystal microbalance (QCM) biosensors and on medical imaging technologies such as tomography, MRI, and NMR. Written in the format of...
Humana Press, 2014. — 591 p. Series: Methods in Molecular Biology, № 1131 The book expands upon the previous edition with current, detailed modern approaches to isolate and characterize monoclonal antibodies against carefully selected epitopes. This edition includes new chapters covering the key steps to generate high quality monoclonals via different methods, from antigen...
Humana Press, 2022. — 301 p. — (Methods in Molecular Biology 2490). — ISBN 978-1-0716-2280-3. This book aims to deliver the most up-to-date protocols in using epiblast stem cells (EpiSC) to answer critical questions on mammalian development. Beginning with a section on the spectrum of mouse primed pluripotent stem cells, the volume continues with chapters on toolkits to...
Humana Press, 2008. — 482 p. — (Methods in Molecular Biology 416). The book contains a comprehensive collection of experimental and computational strategies and techniques for microbial genome-scale essentiality studies, developed and presented by the leading groups in the field. In addition to wet-lab protocols, the book describes (i) statistical methods essential for planning...
Humana Press, 2008. — 310 p. — (Methods In Molecular Biology 494). Due to their high specificity and low toxicity profile, peptides have once again become central to the development of new drugs. In Peptide-Based Drug Design: Methods and Protocols, expert researchers provide a handbook which offers a selection of research and production tools suitable for transforming a...
Humana Press, 2017. - 222 p. Volume 1491 of Methods in Molecular Biology. This volume focuses on explorative activity-based proteomics,biomedical applications of activity-based proteomics, and chemical strategies in activity-based proteomics providing a concise overview of activity-based protein profiling. Written in the highly successful Methods in Molecular Biology series...
Humana Press, 2015 — 2nd Ed. — 375p. — (Methods in Molecular Biology 1261) — ISBN: 978-1-4939-2230-7 (eBook), 978-1-4939-2229-1 (Hardcover).
This updated and expanded volume reflects the current state of the structural protein field with improved and refined protocols that have been applied to particularly challenging proteins, notably integral membrane proteins and...
3rd edition. — Humana Press, 2021. — 350 p. — (Methods in Molecular Biology, 2305). — ISBN 978-1-0716-1405-1. This updated and expanded volume reflects the current state of the structural protein field with improved and refined protocols that have been applied to particularly challenging proteins. Beginning with a section on structural bioinformatics, the book continues with...
3rd edition. — Humana Press, 2021. — 350 p. — (Methods in Molecular Biology, 2305). — ISBN 978-1-0716-1405-1. This updated and expanded volume reflects the current state of the structural protein field with improved and refined protocols that have been applied to particularly challenging proteins. Beginning with a section on structural bioinformatics, the book continues with...
New York: Humana Press, 2014. — 385 p. — (Methods in Molecular Biology 1075). The book takes the researcher from the bench top through the imaging process, to the page. Protocols for the preparation of tissues from many model organisms including worms, flies and mice have been included as well as chapters on confocal imaging of living cells, three dimensional analysis, and the...
2nd ed. — Humana, 2025. — 234 p. — ISBN 978-1071645413 This volume provides readers with an overview on the latest advances in cancer vaccines, and the major issues that need to be addressed in their development. The chapters in this book cover recent progress in immunotherapy; methods for generating relevant mouse models and engineering viral-based vaccines; oncolytic virus;...
Humana Press, 2014. — 419 p. Series: Methods in Molecular Biology, № 1138 Infection by flaviviruses such as dengue virus serotypes (DENV 1-4), Japanese encephalitis virus (JEV), tick-borne encephalitis virus (TBE), yellow fever virus (YFV) and West Nile virus (WNV) impact millions of lives and cause tens of thousands of mortalities each year. Dengue is a global public health...
2nd Edition. - Humana Press, 2018. - 419 p. - Volume 1782 of Methods in Molecular Biology. This second edition volume expands on the previous edition with chapters discussing the latest developments and research initiatives in mitochondrial functions. The chapters in this book explore topics such as high-resolution fluorespirometry and OXPHOS protocols in human cells, analysis of...
Humana Press, 2012. - 340 p. - Increasing interest in mitochondrial bioenergetics is being driven by the impact of drug and environmental chemical-induced disturbances of mitochondrial function as well as hereditary deficiencies and the progressive deterioration of bioenergetic performance with age. These initiatives have fostered the investigation of genetic and environmental...
3rd ed. — Humana Press, 2024. — 300 p. This updated volume provides a concise set of protocols on the conceptual principles and practical considerations pertinent to research in mitochondrial function. Driven by the impact of drug- and environmental chemical-induced disturbances on mitochondrial function, hereditary deficiencies, and the age-related decline in bioenergetic...
New York: Humana Press, 2015. — 215 p. In this volume expert researchers in the field detail the many methods for further research into regulation of mitochondrial function. Chapters focus on mitochondria with other cellular components, discussing how these interactions influence the dynamics of mitochondrial structure and biogenesis. Written in the highly successful Methods in...
Humana Press, 2010. — 232 p. — (Neuromethods 46) — ISBN: 978-1-60761-540-8 Affecting over a hundred million individuals worldwide, retinal diseases are among the leading causes of irreversible visual impairment and blindness, and appropriate study models, especially animal models, are essential to furthering our understanding of the etiology, pathology, and progression of these...
Humana Press, 2018. — 224 p. — (Methods in Molecular Biology 1692). — ISBN: 978-1-4939-7400-9. This detailed volume gathers a comprehensive collection of methods, protocols, and procedures used for the identification, characterization, and selection of cancer stem cells. These include surface marker expression, side population, spheres formation, ALDH activity, and numerous...
Humana Press, 2010. — 363 p. — (Methods in Molecular Biology 591) — ISBN: 978-1-60761-403-6 Now a routine tool in biomedical and life science research, live cell imaging has made major progress enabling this core biochemical, cell, and molecular biology technique to become even more powerful, versatile, and affordable. In Live Cell Imaging: Methods and Protocols, a panel of...
Humana Press, 2022. — 861 p. — (Methods in Molecular Biology 2509). — ISBN 978-1-0716-2380-0. This detailed volume explores newly-developed methods in PIWI-interacting RNAs (piRNAs) research, methods currently applied to other ncRNAs involved in nuclear regulation which can be used to study piRNAs, and piRNA methods applied in non-classical organisms. It also includes several...
Humana Press, 2022. — 396 p. — (Methods in Molecular Biology 2509). — ISBN 978-1-0716-2379-4. This detailed volume explores newly-developed methods in PIWI-interacting RNAs (piRNAs) research, methods currently applied to other ncRNAs involved in nuclear regulation which can be used to study piRNAs, and piRNA methods applied in non-classical organisms. It also includes several...
Humana Press, 2018. — 216 p. — ISBN10: 1493976516, ISBN13: 978-1493976515. This volume provides a collection of methods and protocols detailing extracellular RNA and hopes to be a useful resource for those interested in this exciting and emerging field. Chapters are divided into four sections covering an overview of the field, approaches for the isolation of exRNA, protocols...
Humana Press, 2018. — 216 p. — ISBN10: 1493976516, ISBN13: 978-1493976515. This volume provides a collection of methods and protocols detailing extracellular RNA and hopes to be a useful resource for those interested in this exciting and emerging field. Chapters are divided into four sections covering an overview of the field, approaches for the isolation of exRNA, protocols...
Humana Press, 2005. — 392 p. — (Methods in Molecular Biology 301). — eISBN 1-59259-895-1. Biochemical Methods to Study the Ubiquitin–Proteasome System Small-Molecule Inhibitors of Proteasome Activity Purification of E1 and E1-Like Enzymes Assays for RING Family Ubiquitin Ligases Ubiquitin Chain Synthesis Purification of Proteasomes, Proteasome Subcomplexes, and...
Humana Press, 2014. — 241 p. Series: Methods in Molecular Biology, № 1096 An up-to-date view of molecular mechanisms for investigating microbial communities and their biological activities, this new volume looks at recent advances that are having a big impact on the field such as metagenomics and other “omics” technologies, NanoSIMS, as well as stable isotope probing and more....
Humana Press, 2013. — 255 p. Series: Methods in Molecular Biology, № 990 Meiosis is one of the most critical processes in eukaryotes, required for continuation of species and generation of new variation. In plants, meiotic recombination is by far the most important source of genetic variation. In this volume, expert researchers in the field detail methods for molecular...
Humana Press, 2020. — 477 p. — (Neuromethods 153). — ISBN: 978-1-0716-0426-7. This volume explores major light microscopic imaging modalities that can be used to view nervous tissue, and discusses the steps needed to use each of them, and ways to interpret the data. The chapters in this book cover topics such as atlasing of insect brain; neuroanatomical tracing through...
New York: Humana Press, 2020. — 356 p. This volume aims to cover all major methodological aspects of research into purinergic signaling and to provide a foundation for studying them at molecular, biochemical, pharmacological, and physiological levels. Chapters guide readers through current knock-out and knock-in mouse models, in silico modeling, knock down purinoceptor...
Humana Press, 2018. — 360 p. — (Methods in Molecular Biology 1769). — ISBN 978-1-4939-7779-6. This detailed book presents an up-to-date view on methods and experimental approaches developed to identify and explore the chromothripsis phenomenon. Beginning with a section exploring the genesis and impact of chromothripsis, the collection continues by covering the identification of...
Springer Science+Business Media LLC, 2017. — 335 p. — (Methods in Molecular Biology 1560) — ISBN: 1493967878 The story of histochemistry goes a long way back and, in the last 150 years, has evolved in parallel with the operating instruments, from bright field microscopy to super-resolution microscopy. In this evolution resides the uniqueness of this science. Compared to the...
Humana Press, 2021. — 284 p. — (Methods in Molecular Biology 2567). — ISBN 978-1-0716-2678-8. This detailed volume collects updates on the technical advances in hematopoietic stem cell research and incorporates new techniques focused on the molecular/genetic, cellular, and whole organism levels. Exploring methods that apply stress to hematopoiesis, the book also contains...
Humana Press, 2005 — 452 p. — eISBN 1-59259-827-7 Series: Methods in Molecular Biology, № 286 Transgenic Plants: An Historical Perspective Transformation Plant Transformation: Agrobacterium-Mediated Gene Transfer Production Of Hairy Root Cultures And Transgenic Plants By Agrobacterium Rhizogenes-Mediated Transformation Stable Transformation Of Plant Cells By Particle...
2nd edition. — Humana Press, 2021. — 164 p. — (Methods in Molecular Biology 2319). — ISBN 978-1-0716-1479-2. This second edition brings together a variety of method used in the field of cardiovascular development research. Chapters guide readers through protocols on lymphatic development, lymphatic staining techniques, lymphatic endothelial, tissue clearing techniques, and...
Humana Press, 2017. — 359 p. — (Methods in Molecular Biology 1556). This volume provides leading-edge protocols in the study of the molecular and cellular biology of muscle stem cells. Chapters detail current and updated methods for muscle stem cell isolation, culture, molecular analysis, cellular analysis, and reintroduction in vivo as well as protocols for studying myogenic stem...
Humana Press, 2017. — 289 p. Series: Methods in Molecular Biology, № 1602 The International Committee on Taxonomy of Viruses (ICTV) classifies RNA viruses as those that belong to Group III, Group IV, or Group V of the Baltimore classification system and contain ribonucleic acid (RNA) as genetic material throughout their entire life cycle. Group III includes double-stranded RNA...
Humana Press, 2021. — 396 p. — (Neuromethods 160). — ISBN: 978-1-0716-0911-8. This volume looks at major clinical trials for motor and non-motor symptoms in Parkinson’s Disease (PD) and covers important aspects, including trial design, sample selection, and outcome selection. Chapters in this book discuss topics such as toxin-based rodent or genetic models of PD; clinical...
Humana Press, 2018. - 369 p. - Volume 1665 of Methods in Molecular Biology. The aim of this volume is to provide a broad overview of single-molecule approaches applied to biomolecules. Chapters in Single Molecule Techniques: Methods and Protocols, Second Edition detail the most widely used single-molecule techniques, such as DNA, DNA-binding proteins, motor proteins, and are...
2nd Edition. — Springer Science+Business Media LLC, 2017. — 342 p. – (Methods in Molecular Biology 1570) — ISBN: 978-1-4939-6838-1 Nanoscience and technology focuses on the synthesis and application of structures that have at least one dimension on the sub-100 nm length scale. It deals with investigating the fundamental properties of such structures, which usually differ...
Humana Press, 2021. — 595 p. — (Methods in Molecular Biology 2183). — ISBN 978-1-0716-0794-7. This volume discusses the vaccine development process and the role delivery concepts contribute to a global goal of effective health outcomes. The chapters in this book cover a wide range of topics such as antigen discovery methods; genetic and protein antigen preparation; preparation...
2nd Edition. - Humana Press, 2019. - 270 p. - Volume 1972 of Methods in Molecular Biology. This second edition volume provides a valuable source of information on the application of capillary electrophoresis (CE) and the many different aspects of clinical medicine. Chapters divided into seven parts focus on applications in clinical chemistry and small molecule analysis,...
Humana Press, 2013. — 305 p. Series: Methods in Molecular Biology, № 919 Capillary electrophoresis (CE) is a powerful and rapid tool for performing complex analyses of a number of different molecular species ranging from small inorganic ions to large nucleic acid fragments and proteins. It is quickly becoming established as a useful tool in clinical medicine due to its...
Humana Press, 2023. — 360 p. — (Neuromethods 198). — ISBN 978-1-0716-3217-8. This volume explores the latest experimental techniques in animal models of PTSD and humans affected by PTSD. The methods discussed in this book cover topics such as translational research; addressing sex differences; highlighting the state-of-the-art of biomarker discovery in the development and...
Humana Press, 2022. — 231 p. — (Methods in Molecular Biology 2387). — ISBN 978-1-0716-1778-6. This volume explores the latest techniques used to study Mycobacterium ulcerans, and more specifically M. ulcerans disease (Buruli ulcer). The chapters in this book are organized into three parts and cover methods for the detection of M. ulcerans and the analysis of host-pathogen...
Humana Press, 2022. — 479 p. — (Methods in Molecular Biology 2387). — ISBN 978-1-0716-1779-3. This volume explores the latest techniques used to study Mycobacterium ulcerans, and more specifically M. ulcerans disease (Buruli ulcer). The chapters in this book are organized into three parts and cover methods for the detection of M. ulcerans and the analysis of host-pathogen...
Humana Press, 2021. — 1789 p. — (Methods in Molecular Biology 2216). — ISBN 978-1-0716-0978-1. This volume provides readers with an protocol collection and wide-ranging recommendations for preclinical renal MRI used in translational research. The chapters in this book are interdisciplinary in nature and bridge the gaps between physics, physiology, and medicine. They are...
Humana Press, 2021. — 713 p. — (Methods in Molecular Biology 2216). — ISBN 978-1-0716-0977-4. This volume provides readers with an protocol collection and wide-ranging recommendations for preclinical renal MRI used in translational research. The chapters in this book are interdisciplinary in nature and bridge the gaps between physics, physiology, and medicine. They are designed...
Humana Press, 2013. — 226 р. — (Methods in Molecular Biology 1073). — ISBN: 978-1-62703-625-2 In Synthetic Biology, expert researchers in the field provide the latest developments in molecular biology techniques used in Synthetic Biology. Focusing on computational tools that will aid in systematising the design and construction of parts and systems. Written in the highly...
Humana Press, 1990. — 557 р. — ISBN: 0685382125 Series: Methods in Molecular Biology, №6 Selection of Media for Tissue and Cell Culture Growth Determination and Medium Analysis Measurements of Viability Suitable for Plant Tissue Cultures Cryopreservation of Plant Cells Hormonal Control of Growth and Development The Initiation and Maintenance of Callus Cultures
Humana Press, 2012. - 424 p. Серия: Methods in Molecular Biology, Volume 794 Even though they are present in nature, non-proteinogenic amino acids are usually defined as unnatural or non-natural. Beside their structural diversity, interest in these compounds is due to their occurrence in nature, their biological properties, the analytical aspects, their use as probes, and their...
Humana Press, 2016. — 289 p. — (Methods in Molecular Biology. Vol. 1459) — ISBN: 1493938029 This volume presents relevant background information to understanding the molecular basis governing unconventional protein secretion (UPS), and in particular explores the latest techniques and protocols that have been successfully applied for the study of this topic. Detailed chapters...
Third Edition. — Humana Press, 2014. — 489 p. Series: Methods in Molecular Biology, № 1104 The book constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture from lab to final production. The volume is divided into five parts that...
Humana Press, 2009. — 358 p. — Methods in Molecular Biology, №537 The storage, processing, description, transmission, connection, and analysis of the waves of new genomic data have made bioinformatics skills essential for scientists working with DNA sequences. In Bioinformatics for DNA Sequence Analysis, experts in the field provide practical guidance and troubleshooting advice...
New York: Humana Press, 2015. — 519 p. — (Methods in Molecular Biology). This volume presents the latest developments of the main pillars of protein analysis, such as sample preparation, separation and characterization. The book begins by describing basic but important sample preparation protocols. It then goes on to describe more sophisticated procedures on enriching specific...
New York: Springer, 2020. — 322 p. This volume provides recent advances in the field of biophysics of membrane proteins. Chapters are divided into several parts: detailing biochemistry and functional analysis, experimental and theoretical structural determinations, membrane protein dynamics, and conformation studies. Written in the highly successful Methods in Molecular Biology...
Humana Press, 2017. — 366 p. — (Methods in Molecular Biology 1538). This detailed volume collects protocols for experimentation into how neurons connect to produce the extraordinary functionalities of the nervous system. Contributed by experts and pioneers in their respective techniques, the book covers synapses in the brain and in culture, their constituents, their structures,...
Humana Press, 2007. — 332 p. — (Methods in Molecular Biology 210). Databases HLA Informatics: Accessing HLA Sequences from Sequence Databases Accessing HLA Sequencing Data Through the 6ace Database Polymorphism in Classical and Nonclassical HLA Genes HLA Typing by Restriction Fragment Length Polymorphism Analysis PCR-Restriction Fragment Length Polymorphism Typing of Class I...
Humana Press, 2016 — 3rd Ed. — 383p. — (Methods in Molecular Biology 1354) — ISBN: 978-1-4939-3046-3 (eBook), 978-1-4939-3045-6 (Hardcover). This third edition volume expands upon the previous editions with new research topics and numerous leading-edge HIV protocols. This book contains chapters that cover various aspects of HIV-1 replication and are helpful for investigating...
Humana Press, 2015 — 296p. — (Methods in Molecular Biology 1272) — ISBN: 978-1-4939-2336-6 (eBook), 978-1-4939-2335-9 (Hardcover). This volume explores the considerable efforts that have been directed towards the development of G Protein-Coupled Receptors (GPCR) screening assays in order to disclose GPCR acting compounds, elucidate signaling mechanisms or evaluate compound’s...
Humana Press, 2020. — 417 p. — (Methods in Molecular Biology 2136). — ISBN: 978-1071604663 This volume details Group A Streptococcus (GAS) research and provides the reader with an extensive collection of research protocols within this important field. Chapters guide readers through standard genetic protocols such as whole genome sequencing, transcriptome analyses, proteome...
Humana Press, 2020. — 417 p. — (Methods in Molecular Biology 2136). — ISBN: 978-1071604663. This volume details Group A Streptococcus (GAS) research and provides the reader with an extensive collection of research protocols within this important field. Chapters guide readers through standard genetic protocols such as whole genome sequencing, transcriptome analyses, proteome...
Humana Press, 2015. — 349 p. — (Methods in Molecular Biology 1239). This new edition explores current and emerging mutagenesis methods focusing specifically on mammalian systems and commonly used model organisms through comprehensive coverage and detailed protocols. Since the first edition, major advances and discoveries have made chromosomal mutagenesis a widely used technique...
Humana, 2016. — 405 p. This book provides coverage, methodology, and laboratory protocols on the more essential aspects of protein tyrosine phosphatase (PTP) function and regulation, including the use of standardized in vitro functional assays, suitable cell systems, and animal and microorganism models. Chapters covering state-of-the-art technical approaches suitable to...
ITexLi, 2022. — 275 p. — ISBN 183968805X, 9781839688058, 1839688041, 9781839688041, 1839688068, 9781839688065. This book covers broad but important aspects of animal modeling for scientific medicine as well as for translational systems and biological sciences. Alternative methods such as cell culture and in vitro experiments that do not require the sacrifice of an animal are...
Humana Press, 2019. — 359 p. — (Methods in Molecular Biology 1917). — ISBN: 978-1-4939-8991-1 This volume provides readers with wide-ranging coverage of CRISPR systems and their applications in various plant species. The chapters in this book discuss topics such as plant DNA repair and genome editing; analysis of CRISPR-induced mutations; multiplexed CRISPR/Cas9 systems;...
Humana Press, 2014 — 2nd Ed. — 551p. — (Methods in Molecular Biology 1124) — ISBN: 978-1-62703-845-4 (eBook), 978-1-62703-844-7 (Hardcover).
Neutrophils, the most abundant white cells in humans, serve as the primary cellular defense against infection. Neutrophil Methods and Protocols , Second Edition provides a concise set of protocols written by leading researchers in the...
Humana Press, 2013. — 337 p. Series: Methods in Molecular Biology, № 969 Synthetic mRNA is an attractive tool for mammalian cell reprogramming that can be used in basic research, as well as in clinical applications. Present mRNA in vitro synthesis is a rather simple procedure, which delivers a high yield of quality product. Various modifications may be introduced into the mRNA...
Humana Press, 2019. - 252 p. - Volume 1925 of Methods in Molecular Biology. This volume looks at the latest developments in studying calcium signaling. The chapters in this book discuss topics such as in vitro and in vivo analyses in model systems; methods to measure Ca 2+ in different subcellular compartments; single Ca 2+ channels; methods to measure cellular ROS and ATP; and...
Springer, 2018. — 387 p. — (Methods in Molecular Biology 1783). — ISBN: 1493978330. This volume provides experimental and bioinformatics approaches related to different aspects of gene expression analysis. Divided in three sections chapters detail wet-lab protocols, bioinformatics approaches, single-cell gene expression, highly multiplexed amplicon sequencing, multi-omics...
Springer, 2018. — 387 p. — (Methods in Molecular Biology 1783). — ISBN: 1493978330. This volume provides experimental and bioinformatics approaches related to different aspects of gene expression analysis. Divided in three sections chapters detail wet-lab protocols, bioinformatics approaches, single-cell gene expression, highly multiplexed amplicon sequencing, multi-omics...
2nd ed. — Humana, 2025. — 373 p. — ISBN-13 978-1071642757 This second edition volume expands on the previous edition with updates on the latest methodologies in the transcriptomics field. The chapters in this book cover topics such as spatial omics, long-read sequencing technology, tissue microarrays, analysis of saliva and extracellular vesicles, machine learning and...
New York: Humana, 2022. — 272 p. This volume provides an overview of methods for plant species, mainly species with high economic value, to achieve in vitro propagation through somatic embryogenesis. Chapters guide readers through somatic embryogenesis protocols, somatic embryos in plants, and new methodologies that are used out to solve in vitro recalcitrance of many species...
Cardiff: Humana Press, 2021. — 871 p. This volume provides detailed, up-to-date methods used in research on Atherosclerosis. Chapters guide readers through an overview of the pathogenesis of atherosclerosis and model systems together with in vitro, ex vivo, in vivo and emerging methods in atherosclerosis research. Written in the highly successful Methods in Molecular Biology...
Humana Press, 2007. — 438 p.
Series: Methods in Molecular Biology, № 382
Microarray Technology, Volumes 1 and 2, present information in designing and fabricating arrays and binding studies with biological analytes while providing the reader with a broad description of microarray technology tools and their potential applications. The first volume deals with methods and...
Humana Press, 2007. — 469 p.
Series: Methods in Molecular Biology, № 381
Microarray Technology, Volumes 1 and 2, present information in designing and fabricating arrays and binding studies with biological analytes while providing the reader with a broad description of microarray technology tools and their potential applications. The first volume deals with methods and...
Humana Press, 2017. — 247 p. — (Methods in Molecular Biology 1509). This volume includes comprehensive descriptions of miRNA biogenesis and their role in the development and progression of various human diseases. The first few chapters of MicroRNA Profiling: Methods and Protocols discuss the effects of over-expressing and repressing of a target miRNA and their effects on cell...
Humana Press, 2022. — 368 p. — (Methods in Molecular Biology 2316). — ISBN 978-1-0716-1463-1. This volume explores the latest methods used by researchers to study the detection, characterization, and various aspects of viroids. The chapters in this book are organized into seven parts and cover topics such as detection methods based on the biology of viroids; detection...
Springer, 2013. — 400 p. — (Methods in Molecular Biology 1033). — ISBN: 1627034862 Membrane proteins and membrane lipids form complex interactive systems that are highly dynamic and able to be studied only by combinations of different in vivo and in vitro techniques? In Membrane Biogenesis: Methods and Protocols, experts in the field present a broad collection of methods to...
Humana Press, 1998. — 259 p.
Series: Methods in Molecular Biology, № 86
The book offers biological investigators a powerful and highly useful collection of up-to-date methods for the isolation of RNA from a variety of sources, including bacterial, plant, and mammalian cells. These fine-tuned protocols take both the experienced and inexperienced investigator through the nuances...
New York: Humana Press, 2022. — 907 p. This volume provides detailed technical protocols on current biomedical technologies and examples of their applications and capabilities. Chapters focus on molecular and cellular analytical methods, experimental new drug delivery approaches, guided surgery, implants and tissue engineering. Written in the format of the highly successful...
Humana Press, 2009. — 452 p. — (Methods in Molecular Biology 503). The primary aim of this book is to describe the basic types and the basic elements of biosensors from methods point of view. We tried to include manuscripts that represent the major technologies in the field and to include enough technical detail so that the informed reader can both understand the technology and...
2nd Edition. — Springer Science+Business Media LLC, 2017. — 464 p. — (Methods in Molecular Biology 1571) — ISBN: 978-1-4939-6846-6 This volume provides comprehensive and detailed technical protocols on current biosensor and biodetection technologies and examples of their applications and capabilities. Chapters in Biosensors and Biodetection: Methods and Protocols, Volume 1:...
2nd Edition. — Springer Science+Business Media LLC, 2017. — 579 p. – (Methods in Molecular Biology 1572) — ISBN: 978-1-4939-6910-4 This volume provides comprehensive and detailed technical protocols on current biosensor and biodetection technologies and examples of their applications and capabilities. Chapters in Biosensors and Biodetection: Methods and Protocols Volume 2,...
Humana Press, 2015. — 512 p.
Series: Methods in Molecular Biology, N1256
This volume represents a valuable collection of mobile health (mHealth) emerging technologies. Chapters focus on three main areas of mHealth: technologies for in vitro and environmental testing, mHealth technologies for physiological and anatomical measurements and mHealth technologies for imaging. This...
New York: Humana Press, 2011. — 314 p. Immunology has made significant progress in the past decade, driven forward by rapidly advancing technology and a renewed interest in the vast realm of innate immunity. The receptors that mediate these functions are at the front lines of both protective and regulative roles of the immune system. In Immune Receptors: Methods and Protocols,...
Humana, 2024. — 642 p. This volume contains cutting-edge molecular biology methods on neuroprotective mechanisms and specific preclinical models of the CNS injury, iseases and planning translation. Chapters guide readers through neuropathology, neuroprotection, Alzheimer’s disease, amyotrophic lateral sclerosis, ALS, Huntington’s disease , multiple sclerosis, Parkinson’s...
Humana Press, 2019. — 280 p. — (Methods in Molecular Biology 2019) — ISBN: 978-1-4939-9584-4 This volume explores the latest synthetic procedures for producing receptor-specific retinoids and rexinoids, molecular biology methods, and new technologies to demonstrate the therapeutic activities of molecules. The chapters in this book cover topics such as lentiviral delivery of...
Humana Press, 2019. — 608 p. — (Methods in Molecular Biology 2019) — ISBN: 978-1-4939-9585-1 This volume explores the latest synthetic procedures for producing receptor-specific retinoids and rexinoids, molecular biology methods, and new technologies to demonstrate the therapeutic activities of molecules. The chapters in this book cover topics such as lentiviral delivery of...
New York: Humana Press, 1997. — 271 p. Neurotransmitter Methods is intended as a bench-side companion for researchers who seek to identify, locaHze, or measure neurotransmitters and/or to identify sites of neurotransmitter action. Each method is detailed in a user-friendly "recipe" format and the protocols are accompanied by extensive notes to high light and explain crucial...
Humana Press, 2023. — 513 p. — (Methods in Molecular Biology 2673). — ISBN 978-1-0716-3238-3. This volume explores computational vaccine design and the technologies that support it. Chapters have been divided into four parts detailing immunonics and system immunology, databases, prediction of antigenicity and immunogenicity, and computational vaccinology. Written in the format...
New York: Humana Press, 2015. — 251 p. — (Methods in Molecular Biology 1296). This volume contains state-of-the-art methods tackling all aspects of small non-coding RNAs biology. The book guides readers through customized dedicated protocols and technologies that will be of valuable help to all those willing to contribute deciphering the numerous functions of small non-coding...
2nd Edition. — Springer, 2021. — 266 p. — (Methods in Molecular Biology 2300). — ISBN 978-1-0716-1385-6. This second edition provides new and updated chapters detailing methods tackling all aspects of small non-coding RNAs biology. Chapters guide readers through customized dedicated protocols and technologies that will be of valuable help decipher the numerous functions of...
Humana Press, 2017. — 529p. — (Methods in Molecular Biology 1498). — ISBN: 978-1-4939-6472-7 In vitro mutagenesis remains a critical experimental approach for investigating gene and protein function at the cellular level. This volume provides a wide variety of updated and novel approaches for performing in vitro mutagenesis using such methods as genome editing, transposon (Tn)...
Humana Press, 2024. — 239 p. — (Methods in Molecular Biology 2735). — ISBN 978-1-0716-3526-1. This volume covers the latest advances in technologies that look at familial cardiomyopathies in greater detail, and provides new computational and experimental models that model, study, and detect disease at earlier stages. Together, this allows interdisciplinary research experiments...
Humana Press, 2024. — 239 p. — (Methods in Molecular Biology 2735). — ISBN 978-1-0716-3527-8. This volume covers the latest advances in technologies that look at familial cardiomyopathies in greater detail, and provides new computational and experimental models that model, study, and detect disease at earlier stages. Together, this allows interdisciplinary research experiments...
Humana Press, 2016 — 313p. — (Methods in Molecular Biology 1394) — ISBN: 978-1-4939-3341-9 (eBook), 978-1-4939-3339-6 (Hardcover). This volume presents an overview of contemporary quantitative proteomics methods along with instructions on data interpretation, while providing examples on how to implement proteomics into systems biology. Written in the highly successful Methods...
Humana Press, 2018. — 433 p. — (Methods in Molecular Biology 1799). — ISBN 978-1-4939-7895-3. This book provides researchers the opportunity to investigate type-2-associated diseases in their laboratories. Beginning with chapters describing various models of type-2 immunity, the volume then continues by detailing cellular protocols designed to identify, characterize, and assess...
2nd Edition. — New York: Humana Press, 2022. — 216 p. This second edition provides new and updated methods and protocols for studying tumor angiogenesis in vitro and in vivo. Chapters detail morphological aspects of tumor angiogenesis, aortic ring, ex vivo tissue culture model for anti-angiogenic drug testing, transgenic zebrafish, orthotopic models of ovarian cancer, and...
New York: Humana Press, 2015. — 285 p. The book provides a range of techniques for studying vascular morphogenesis in vivo and in vitro, reflecting advances in the field. Endothelial cell signaling is currently believed to promote fundamental cues for cell fate specification, embryo patterning, organ differentiation and postnatal tissue remodeling. Understanding the concept of...
2nd edition. — Humana Press, 2021. — 297 p. — (Methods in Molecular Biology 2206). — ISBN 978-1-0716-0915-6. This detailed new edition aims to provide a range of methods and protocols for studying vascular morphogenesis in vivo and in vitro to reflect up-to-date advances in the field. Of note, the book explores endothelial cell signaling is currently understood to promote...
Humana Press, 2011. - 337 p. - Methods in Molecular Biology, Volume 766. - Few problems in protein biochemistry have proven to be as challenging and recalcitrant as the molecular description of nitrogenase, the catalyst of one of the most remarkable chemical transformations in biological systems: the nucleotide-dependent reduction of atmospheric dinitrogen to bioavailable...
Humana Press, 2019. — 292 p. — (Methods in Molecular Biology 2056) — ISBN: 978-1-4939-9783-1 This detailed book contains techniques to explore the unusual properties of the peculiar microsatellites known as trinucleotide repeats. Beginning with technical challenges raised by secondary structure-forming repeats and their propensity to contract and expand over time, the book...
Humana Press, 1995. - 412 p. ISBN: 0896032728 Series: Methods in Molecular Biology, №39 Baculovirus Expression Protocols offers both industrial and university-based researchers a comprehensive comilation of the latest baculovirus techniques along with step-by-step instructions and time-saving techniques. The contributors-leading authorities in the field-present the assorted...
Humana Press, 2014 — 475p. — (Methods in Molecular Biology 1110) — ISBN: 978-1-4614-9408-9 (eBook), 978-1-4614-9407-2 (Hardcover).
In Flower Development: Methods and Protocols , researchers in the field detail protocols for experimental approaches that are currently used to study the formation of flowers, from genetic methods and phenotypic analyses, to genome-wide...
2nd edition. — Humana Press, 2023 — 586 p. — (Methods in Molecular Biology 2686). — ISBN 978-1-0716-3298-7. This second edition details new and updated protocols for experimental approaches that are currently used to study the formation of flowers. Chapters guide readers on genetic methods, phenotypic analyses, genome-wide experiments, modeling, and system-wide approaches....
Third Edition. — Humana Press, 2014. — 321 p. Series: Methods in Molecular Biology, № 1143 The book explores innovative approaches and technologies used to design, deliver, and enhance the efficacy of DNA vaccines. Featuring applications which should be of great value in moving vaccines from research to clinic, this detailed volume includes sections on DNA vaccine design and...
Springer US; Humana, 2020. — 245 p. — (Methods in Molecular Biology 2081). — ISBN-13 9781493999408. This volume provides detailed protocols and methodologies required to perform bioluminescent imaging in multiple stages, enabling the reader to integrate this technology into their laboratory-based imaging experiments. Written in the highly successful Methods in Molecular Biology...
New York: Springer, 2024. — 306 p. This volume provides comprehensive methods on RNA design. Chapters details traditional RNA design in secondary structure, RNA design in tertiary structure, and RNA design applications and assessments. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of...
2nd Edition. — Humana Press, 2016. — 305 p. — ISBN: 1493963597 Series: Methods in Molecular Biology, №1476 This second edition provides 21 new chapters on methods used in laboratories for investigating the physiology and molecular genetics of the pathogen Clostridium difficile. Chapters detail up-to -date experimental techniques for gene editing and transcriptional analysis...
3rd edition. — New York: Humana Press, 2020. — 299 p. This fully updated edition provides the most recent advances in cancer cell signaling knowledge combined with some discussion of the current challenges and prospects in cancer therapy. Beginning with an important section on cancer resistance, the main obstacle to effective cancer therapy, the book continues with chapters...
Humana Press, 2014. — 277 p. Series: Methods in Molecular Biology, № 1165 The book brings together the most recent advances in cancer cell signaling knowledge along with recent progress in the development of novel strategies and experimental cell models to study human cancer. Opening with a section on targeting cancer cell metabolism as an anticancer strategy, this detailed volume...
Springer, 2019. — 353 p. — (Methods in Molecular Biology 1889). — ISBN: 1493988964. This detailed volume collects many of the common experimental approaches used to study myogenesis. It covers subjects ranging from isolation and purification protocols, manipulation of muscle cells, transcriptomics and proteomics, metabolism and exercise, and tissue engineering. Presented...
Humana Press, 2014 — 377p. — (Methods in Molecular Biology 1125) — ISBN: 978-1-62703-971-0 (eBook), 978-1-62703-970-3 (Hardcover).
In Polyadenylation: Methods and Protocols , expert researchers in the field detail many of the protocols which are now commonly used to study polyadenylation. Focusing on recent advances in the fast-moving polyadenylation filed, that has recently...
New York: Humana Press, 2007. — 593 p. — (Methods in Molecular Biology 362). The aim of Circadian Rhythms is to provide a resource that can be adopted by several types of users: those who are new to circadian biology, those who are already active in the field but are interested in learning new techniques and researchers who are considering moving to a new a model system or...
Humana Press, 2014. — 597 p. Series: Methods in Molecular Biology, № 1154 The book is intended for all practitioners of reproductive medicine and ART, as well as for embryologists and reproductive, developmental, cell and molecular biologists and others in the biomedical sciences. The volume presents straight-forward manner best practice approaches for overcoming a host of...
Humana Press, 2021. — 439 p. — (Methods in Molecular Biology, 2262). — ISBN 978-1-0716-1189-0. This volume compiles new experimental approaches and concepts focusing mostly, but not solely, on ways to manipulate and regulate Ras activity and its downstream signaling output. Chapters detail standard methodologies, biochemical methods, Ras processing trafficking and localization,...
Humana Press, 2021. — 439 p. — (Methods in Molecular Biology, 2262). — ISBN 978-1-0716-1189-0. This volume compiles new experimental approaches and concepts focusing mostly, but not solely, on ways to manipulate and regulate Ras activity and its downstream signaling output. Chapters detail standard methodologies, biochemical methods, Ras processing trafficking and localization,...
Humana Press, 2016 — 314p. — (Methods in Molecular Biology 1395) — ISBN: 978-1-4939-3347-1 (eBook), 978-1-4939-3345-7 (Hardcover). This volume discusses the latest advancements and technologies used in cancer drug resistance research. Cancer Drug Resistance: Overviews and Methods contains chapters that cover topics such as: studying the mechanics of resistance to DNA damaging...
Humana, 2013. — 173 p. Remarkably, while G protein-coupled receptors (GPCRs) are highly prevalent in animals and yeast, very few candidate GPCRs have been identified in plants. In G Protein-Coupled Receptor Signaling in Plants: Methods and Protocols, experts in the field describe techniques used in the study of small GTPases and related proteins. Beginning with a chapter on...
Humana, 2000. — 272 p. The past decade has seen an extraordinary growth in research interest in neurotrophic factors, and the study of the neurotrophin family has led this activity. Nevertheless, this area of research has often struggled as a result of techniques that were either inadequate or just emerging from other research fields and disciplines. Neurotrophin Protocols has...
Humana Press, 2014. — 289 p. Series: Methods in Molecular Biology, №1079 From basic performing of sequence alignment through a proficiency at understanding how most industry-standard alignment algorithms achieve their results, Multiple Sequence Alignment Methods describes numerous algorithms and their nuances in chapters written by the experts who developed these algorithms....
Humana, 2025. — 343 p. — ISBN-13 : 978-1071642832 This detailed volume explores various methods through which nutrient sensing can be studied, particularly in multicellular eukaryotes. Beginning with a section on the monitoring of cellular energy status by sensing of adenine nucleotides, the book continues by delving into numerous techniques for researching both intracellular...
Humana Press, 2017. - 209 p. Volume 1564 of Methods in Molecular Biology. This detailed volume compiles state-of-the-art methodologies for the study of brassinosteroid hormones, contributed by recognized researchers in the field, in order to bring together different experimental and theoretical biology techniques for addressing the questions of how brassinosteroids function in...
Humana Press, 2021. — 495 p. — ISBN: 1071608754 This book provides an overview of methods and experimental protocols that are currently used to analyze the presence and abundance of non-canonical DNA nucleotides in different biological systems. Focusing particularly on the newly discovered and less studied DNA modifications that are enzymatically produced and are likely to play...
Humana Press, 2015. — 249 p. — (Methods in Molecular Biology 1326) — ISBN: 978-1-4939-2838-5 This volume provides detailed molecular analysis of the associated pathways and cell types involved in Celiac Disease, and embraces new technologies such as next generation sequencing. The book is divided into three parts: Part I gives a background overview with a number of reviews to...
Humana Press, 2018. — 251 p. — (Methods in Molecular Biology 1861). This book details recently developed technologies and conventionally employed cytological proceduresfor the study of X-Chromosome Inactivation. Chapters detail live imaging, bioinformatic methods, fluorescence in situ hybridization, and immunofluorescence, and procedures to optimize the study of molecular...
Humana Press, 2012. — 573 p. The development of the hybridoma technology created the possibility to obtain unlimited amounts of monoclonal antibodies (mAb) with high specificity and affinity for any target and to introduce mAbs in a wide range of applications; however, the bulky size of mAbs, costly production, and cumbersome engineering hampered regularly their streamlined...
Springer, 2019. — 255 p. — (Methods in Molecular Biology 1944). — ISBN: 978-1-4939-9094-8. This detailed volume compiles state-of-the-art protocols that will serve as recipes for scientists researching collagen, an abundant protein with great importance to health and disease, as well as in applications like food, cosmetics, pharmaceuticals, cosmetic surgery, artificial skin,...
Humana, 2024. — 234 p. — ISBN-13 : 978-1071639092 This volume provides references for methods about the proteomics of microbial communities, also called metaproteomics. Chapters guide readers first through specific protein extractions from different environments and/or ecological niches crowded by heterogeneous microbial communities, then deepening the possible complete...
Humana Press, 2016 — 324p. — (Methods in Molecular Biology 1388) — ISBN: 978-1-4939-3299-3 (eBook), 978-1-4939-3297-9 (Hardcover). This volume provides detailed descriptions of prevailing and novel techniques used by experts in the study of PTEN function in disease and biology. The book begins with chapters exploring methods to detect expression levels of PTEN in normal and...
Humana Press, 2018. — 416 p. — (Methods in Molecular Biology). — ISBN: 9781493969807, 9781493969814. This volume presents protocols that analyze and explore hemorrhagic fever viruses (HFV). This book is divided into 5 parts: Part I begins with an overview on predicting viral pandemics and then covers methods for surveillance, diagnosis, and classification of HFV. This includes...
Boston: Elsevier, 1998. — 398 p. Computational biology is a rapidly expanding field, and the number and variety of computational methods used for DNA and protein sequence analysis is growing every day. These algorithms are extremely valuable to biotechnology companies and to researchers and teachers in universities. This book explains the latest computer technology for analyzing...
Humana Press, 2017. - 449 p. Volume 1529 of Methods in Molecular Biology. The aim this volume is to present the methods, challenges, software, and applications of this widespread and yet still evolving and maturing field. Computational Protein Design, the first book with this title, guides readers through computational protein design approaches, software and tailored solutions...
Humana Press, 2013. - 265 p. - Methods in Molecular Biology v .978. - Whether the pursuit is commercially motivated or purely academic, engineering a novel biological catalyst is an enticing challenge. High-resolution protein structure analysis allows for rational alteration of enzyme function, yet many useful enzyme variants are the product of well-designed selection schemes...
Springer, 2018. — 433 p. — (Methods and Protocols 1883). — ISBN: 1493988816. This volume explores recent techniques for the computational inference of gene regulatory networks (GRNs). The chapters in this book cover topics such as methods to infer GRNs from time-varying data; the extraction of causal information from biological data; GRN inference from multiple heterogeneous...
Humana, 2023. — 318 p. This volume details bioinformatics analysis, biochemical assays, recombinant protein expression and purification, methods to investigate protease activity in cell-based, organoids and in vivo systems, proteomics, transcriptomics, machine learning and novel approaches to target dysregulated protease activity in cancer. Written in the highly successful...
Humana Press, 2019. - 252 p. - Volume 1927 of Methods in Molecular Biology. This volume covers a wide array of topics that will aid researchers in the task of engineering complex biological systems. This book is divided into three parts: Part One discusses the discovery and identification of relevant biosynthetic pathways for engineering; Part Two looks at the development of...
Springer, 2019. — 369 p. — (Methods in Molecular Biology 1886). — ISBN: 149398893X. This book aims to provide examples of applications of atomic force microscopy (AFM) using biological samples, showing different methods for AFM sample preparation, data acquisition and processing, and avoiding technical problems. Divided into two sections, chapters guide readers through image...
2nd edition. — Humana Press, 2023. — 407 p. — (Methods in Molecular Biology 2601). — ISBN 978-1-0716-2854-6. This second edition provides state-of-the-art and novel methods on antibiotic isolation and purification, identification of antimicrobial killing mechanisms, as well as methods for the analysis and detection of microbial responses and adaptation strategies. Antibiotics:...
Humana Press, 2017. — 352 p. — (Methods in Molecular Biology). — ISBN10: 1493966324. — ISBN13: 978-1493966325. This volume provides state-of-the-art and novel methods on antibiotic isolation and purification, identification of antimicrobial killing mechanisms, and methods for the analysis and detection of microbial adaptation strategies. Antibiotics: Methods and Protocols...
Springer Science+Business Media, New York, 2016. — 275 p. — (Methods in Molecular Biology 1454). — ISBN: 1493937871 This volume is the result of an explosion of molecular-based research on Cilia, which began with the discovery of the universality of intraflagellar transport (IFT) and ciliary genomics/proteomics. The chapters in this book cover topics such as: high resolution...
Humana Press, 2011. — 297 p. — (Methods in Molecular Biology 716). — ISBN: 978-1-61779-011-9. Research in the pharmaceutical sciences and medicinal chemistry has taken an important new direction in the past two decades with a focus on large molecules, especially peptides and proteins, as well as DNA therapeutics. In Drug Design and Discovery: Methods and Protocols, leading...
Humana Press, 2023. — 451 p. — (Methods in Molecular Biology 2631). — ISBN 978-1-0716-2989-5. This detailed volume focuses on genotyping and validation in addition to information on how to produce gene edited cells and animals for research. Future advances in biomedical research will benefit greatly from the use of precise gene targeting of transgenes in the genome as CRISPR...
Humana Press, 2023. — 251 p. — (Methods in Molecular Biology 2633). — ISBN 978-1-0716-3003-7. This volume details technologies on recombinant DNA and nucleic acid manipulation that underpin much of the biological sciences and instructions on how to conduct them successfully. Chapters guide the reader through the basics of oligonucleotide synthesis and DNA sequencing;...
3rd Edition. — Springer, 2021. — 215 p. — (Methods in Molecular Biology 2182). — ISBN: 978-1-0716-0791-6. This third edition expands upon the previous edition with current, detailed methods on different aspects, and landmarks advancing knowledge on Salmonella research. With new chapters on molecular assays for detection, identification and serotyping of Salmonella, quantitative...
Humana Press, 2015. — 303 p. Series: Methods in Molecular Biology, № 1225 The book presents detailed methods on a variety of aspects of Salmonella research, focusing on those which provide landmarks for future discovery. Highlighted topics include molecular genotyping, rapid and accurate new methods for sensitive determination of antibiotic resistance profiles, the...
Humana Press, 2022. — 356 p. — ISBN 978-1-0716-2521-7 This detailed volume collects commonly used and cutting-edge methods to analyze alternative splicing, a key step in gene regulation. After an introduction of the alternative splicing mechanism and its targeting for therapeutic strategies, the book continues with techniques for analyzing alternative splicing profiles in...
Humana Press, 2022. — 345 p. — (Methods in Molecular Biology 2480). — ISBN 978-1-0716-2240-7. This volume provided methods and protocols on recombinant protein production in different plant systems, downstream processing, and strategies to optimize protein expression. Chapters guide readers through recombinant protein production in important plant systems, protein recovery and...
Humana Press, 2022. — 813 p. — (Methods in Molecular Biology 2480). — ISBN 978-1-0716-2241-4. This volume provided methods and protocols on recombinant protein production in different plant systems, downstream processing, and strategies to optimize protein expression. Chapters guide readers through recombinant protein production in important plant systems, protein recovery and...
New York: Humana Press, 2015. — 231 p. In this volume expert researchers in the field detail many of the methods which are now commonly used to study RNA. These methods are presented as a guidebook to scientists who are experienced with RNA research and want to brush up on a new technique. Written in the highly successful Methods in Molecular Biology series format, chapters...
Humana Press, 2013. — 587 p. Series: Methods in Molecular Biology, № 955 The basic principle of electron crystallography is to calculate a 3D density map by combining the amplitudes obtained from electron diffraction patterns with the experimental phases calculated from images of two-dimensional crystals of membrane or soluble proteins. This technology is very well developed...
Humana, 2021. — 359 p. — (Methods in Molecular Biology, 2302). — ISBN 978-1-0716-1393-1. This book examines detailed experimental and computational approaches for the analysis of many aspects vital to the understanding of membrane protein structure and function. Readers will receive guidance on the selection and use of methods for over-expression and purification, tools to...
Humana, 2021. — 359 p. — (Methods in Molecular Biology, 2302). — ISBN 978-1-0716-1393-1. This book examines detailed experimental and computational approaches for the analysis of many aspects vital to the understanding of membrane protein structure and function. Readers will receive guidance on the selection and use of methods for over-expression and purification, tools to...
Humana Press, 2016. — 492 p This volume guides readers through the field of systems medicine by defining the terminology, and describing how established computational methods form bioinformatics and systems biology can be taken forward to an integrative systems medicine approach. Chapters provide an outlook on the role that systems medicine may or should play in various medical...
Humana Press, 2000. — 206 p. — (Methods in Molecular Biology 140). In this volume Christine Schneider has assembled a unique collection of readily reproducible protocols for the study of chaperonins, intracellular proteins critical to many biological processes. Written by experienced investigators who have successfully honed their methods to a fineness, the protocols focus on the...
Humana Press, 2004. — 331 p. — (Methods in Molecular Biology 280). Intracellular checkpoint controls constitute a network of signal transduction pathways that protect cells from external stresses and internal errors by means of cell cycle arrest, DNA repair, or apoptosis. Failure of this machinery can lead to embryonic death, genetic diseases, and cancer. In Checkpoint Controls...
Humana Press, 2004. — 333 p. — (Methods in Molecular Biology 281). Intracellular checkpoint controls constitute a network of signal transduction pathways that protect cells from external stresses and internal errors by means of cell cycle arrest, DNA repair, or apoptosis. Failure of this machinery can lead to embryonic death, genetic diseases, and cancer. In Checkpoint Controls...
Humana, 2024. — 503 p. This updated edition explores essential techniques and newly emerged applications used in the expanding field of peptidomics. The opening chapters describe sample preparation and basic techniques. Subsequent chapters delve into quantitative peptidomics, bioinformatics, deep learning approaches, non-human model organisms, and peptide toxins. The last...
2nd edition. — Humana Press, 2023. — 507 p. — (Methods in Molecular Biology 2643). — ISBN 978-1-0716-3047-1. This fully updated volume assembles a comprehensive collection of methods, techniques, and strategies to investigate the molecular and cellular biology of peroxisomes in different organisms. Peroxisome research is on the rise, as novel functions and proteins of this...
Humana Press, 2017. — 234 p. — (Methods in Molecular Biology 1561). This volume covers an array of techniques available for studying peptide-protein docking and design. The book is divided into four sections: peptide binding site prediction; peptide-protein docking; prediction and design of peptide binding specificity; and the design of inhibitory peptides. The chapters in...
Humana Press, 2017. — 605 p. — (Methods in Molecular Biology 1488). This volume focuses on the use of system genetic methods and the use of murine models to study the role of gene variants and environmental factors on human health and disease—what is now often called personalized or precision health care. The protocols in this book will help readers analyze genetic causes of...
Humana Press, 2015. — 243 p. Series: Methods in Molecular Biology, № 1306 This detailed volume addresses recent developments in phosphoproteomic techniques with a particular focus on the plant system. Over the recent decades, proteomic methods were refined to study the significance and dynamics of protein phosphorylation in various biological contexts. However, working with plant...
Humana Press, 2013. — 526 p. Series: Methods in Molecular Biology, № 970 There is a demand for analytical methods that are able to discriminate between enantiomers in order to analyze the enantiomeric purity of compounds from natural or chemical sources not only in pharmaceutical sciences but in any field on bioactive compounds including chemistry, biology, biochemistry,...
Humana Press, 2016 — 2nd Ed. — 306p. — (Methods in Molecular Biology 1410) — ISBN: 978-1-4939-3524-6 (eBook), 978-1-4939-3522-2 (Hardcover). This volume describes prominent methodologies developed by laboratories that have been leading the field of quantitative proteomics by mass spectrometry. The procedures for performing the experiments are described in an easy-to-understand...
Humana Press, 2019. — 253 p. — (Methods in Molecular Biology 1969). — ISBN: 978-1-4939-9201-0. This volume provides methods to analyze the meningococcus and its interactions with biologically relevant host cells and sites, to interrogate the population structure and biology of the meningococcus that defines its capacity to cause disease, and to aid in vaccine development and...
Humana Press, 2003. — 386 p. Series: Methods in Molecular Biology, №228 A collection of key techniques for the study of receptors and transport proteins. The book provides examples of how different membrane proteins can be overexpressed in both prokaryotic and eukaryotic expression systems, how natural and overexpressed proteins can be solubilized from their host membranes, and...
Humana, 2024. — 622 p. This second edition provides new and updated chapters covering computational and mathematical techniques and concepts related to the field of comparative genomics. The topics covered in the chapters range from those that address general techniques and concepts that apply to all organisms to others that are specialized and apply to specific biological...
New York: Humana, 2022. — 330 p. This detailed volume explores a variety of cutting-edge techniques used to interrogate spatial genome organization. Beginning with a section covering the vital chromosome conformation capture (3C) technique, this collection continues with chapters on targeted Hi-C approaches, sequencing-based approaches to assess nuclear environment, as well as...
2nd Edition. - Humana Press, 2017. — 499 p. — (Methods in Molecular Biology 1537). This second edition volume presents a selection of cellular and molecular techniques that can be adapted to cover a range of applications and diseases. The book is divided into three sections: saliva and oral diseases, molecular biosciences, and cell and tissues. The first section contains chapters...
3rd edition. — Humana Press, 2023. — 522 p. — (Methods in Molecular Biology 2588). — ISBN-10 1071627791. — ISBN-13 978-1071627792. This fully revised new edition explores advances in the prevention and treatment of oral diseases. Beyond the updated chapters, the book delves into regenerative biology, gene editing and the use of CRISPR in oral biology, as well as histone...
Humana Press, 2011. — 262 p. — (Methods in Molecular Biology 680) — ISBN: 978-1-60761-900-0 In recent years, molecular imaging techniques have grown to be invaluable tools for molecular biology research and, to a more modest extent, clinical medicine. In Molecular Imaging: Methods and Protocols, expert researchers explore the latest advances in the field, describing a rich...
Humana Press, 2017. — 354 p. — (Methods in Molecular Biology 1578). This volume covers protocols on techniques ranging from MAMP isolations from diverse microorganisms, PRR identifications from different plant species, MAMP-PRR binding, and a series of signaling responses and events revealed by various biochemical, cellular, genetic and bioinformatic tools. Written in the highly...
2nd Edition. - Humana Press, 2019. - 214 p. - Volume 1963 of Methods in Molecular Biology. This fully updated second edition explores protocols that address the most challenging aspects of experimental work in ancient DNA, such as preparing ancient samples for DNA extraction, the DNA extraction itself, and transforming extracted ancient DNA molecules for sequencing library...
New York: Humana Press, 2022. — 328 p. This volume brings together cutting-edge laboratory protocols to characterize the novel fluorescent proteins (FPs) and approaches based on fluorescent proteins that aim to answer some of the key cell biological questions. The book covers topics ranging from the database of fluorescent proteins to their characterization and adaptation to a...
InTech, 2012. - 328 p. - This is a concise book on applied methods of enzymes used in drug testing. The present volume will serve the purpose of applied drug evaluation methods in research projects, as well as relatively experienced enzyme scientists who might wish to develop their experiments further. Chapters are arranged in the order of basic concepts of enzyme inhibition...
Humana Press, 2014. — 309 p. Series: Methods in Molecular Biology, № 1136 The book provides state-of-the-art overviews on the most important approaches currently used in mitosis research spanning from the analysis of single molecules in isolation to their utilization within the complex environment of the cell. The volume is divided into four parts, each focused on methods...
Humana Press, 2023. — 1100 p. — (Methods in Molecular Biology 2638). — ISBN 978-1-0716-3024-2. This thorough volume presents a wide range of existing methods, from the very popular to the more exotic, in the area of plant genotyping. Many methods of plant genotyping were initially developed for medical research, but all genotyping methods, if they are to be successful, should...
Humana Press, 2023. — 464 p. — (Methods in Molecular Biology 2638). — ISBN 978-1-0716-3023-5. This thorough volume presents a wide range of existing methods, from the very popular to the more exotic, in the area of plant genotyping. Many methods of plant genotyping were initially developed for medical research, but all genotyping methods, if they are to be successful, should be...
Humana Press, 2022. — 408 p. — (Methods in Molecular Biology 2473). — ISBN 978-1-0716-2208-7. This detailed volume presents a series of methods exploring membrane trafficking research, ranging from genetics and high-resolution imaging to in vitro biochemical and biophysical assays. Covering virtually all the major trafficking branches, the book delves into the exocytic pathway,...
Humana Press, 2017. — 365 p. — (Methods in Molecular Biology 1650). This volume discusses recent advances in avian and reptilian biology that have caused this diverse field to re-emerge. The chapters in this book are divided into 4 parts: genomics and transcriptomics, genetic manipulation, stem cells, and new model systems. Part I details how to perform genomic and...
Humana Press, 2014. — 215 p. Series: Methods in Molecular Biology, № 1142 The book expands upon the first edition to present new and current techniques for the research of arthritis and related conditions. A compendium of leaders in the field contribute chapters that cover practical research methods such as the intravital multiphoton microscopy technique, techniques for evaluating...
Humana Press, 1995. - 368 p. ISBN: 0896033015 Series: Methods in Molecular Biology, №40 Noncovalent Forces Important to the Conformational Stability of Protein Structures. Degradative Covalent Reactions Important to Protein Stability Fluorescence Spectroscopy. Ultraviolet Absorption Spectroscopy. Circular Dichroism. Infrared Spectroscopy. Identifying Sites of Posttranslational...
Humana Press, 2013. — 234 p. — (Methods in Molecular Biology 1038). The new genetic revolution is fuelled by Deep Sequencing (or Next Generation Sequencing) apparatuses which, in essence, read billions of nucleotides per reaction. Effectively, when carefully planned, any experimental question which can be translated into reading nucleic acids can be applied.In Deep Sequencing Data...
Humana Press, 2020. — 230 p. — (Methods in Molecular Biology 2064) — ISBN: 978-1-4939-9829-6 This volume explores the latest techniques and workflow for the analysis of single cells metabolism. The chapters in this book cover topics such as the development of mass spectrometry-based single cell approaches, Pico-ESI-MS for single-cell metabolomics analysis; laser capture...
Humana Press, 2022. — 244 p. — (Methods in Molecular Biology 2396). — ISBN 978-1-0716-1821-9. This volume looks at the latest techniques used by researchers to study various aspects of plant metabolic engineering. The chapters in this book cover topics such as bioinformatics tools used to discover new genes and pathways; heterologous expression of biosynthetic genes in plant...
Humana Press, 2022. — 506 p. — (Methods in Molecular Biology 2396). — ISBN 978-1-0716-1822-6. This volume looks at the latest techniques used by researchers to study various aspects of plant metabolic engineering. The chapters in this book cover topics such as bioinformatics tools used to discover new genes and pathways; heterologous expression of biosynthetic genes in plant...
Humana Press, 2018. - 346 p. - Volume 1684 of Methods in Molecular Biology. This volume provides readers with the latest techniques and approaches used to study K+ channels. There are a wide range of topics covered in the book, such as channel structure; K+ channels and their roles in physiology and disease; single-molecule FRET; proteins expression levels in vivo; real-time...
3rd Edition. - Humana Press, 2018. - 542 p. - Volume 1779 of Methods in Molecular Biology. This third edition volume expands on the previous editions with many new chapters that cover the latest techniques and topics that were not addressed in the previous volumes. The chapters in this book are divided into three parts: Part One covers in vitro assays that focus on a variety of...
2nd Edition. — Humana Press, 2012. — 541 p. Series: Methods in Molecular Biology, № 849 Amyloid diseases are characterized by the deposition of insoluble fibrous amyloid proteins. The word “amyloid” indicates a starch-like compound, and though a misnomer, continues to be the accepted term for this group of protein conformational disorders. The second edition of Amyloid Proteins...
Humana Press, 2019. - 421 p. - Volume 1851 of Methods in Molecular Biology. This volume presents a diverse collection of methodologies used to study various problems at the protein sequence and structure level. The chapters in this book look at issues ranging from broad concepts like protein space to specifics like antibody modeling. Topics include point mutations, gene...
Humana Press, 2023. — 288 p. — (Methods in Molecular Biology 2599). — ISBN 978-1-0716-2846-1. This volume details protocols emphasizing systems-level approaches that can be applied to genomic analyses. Chapters detail techniques for optimized application in in vivo systems, spatial, physiological, environmental contexts, imaging-based techniques, single-molecule approaches,...
Springer Science+Business Media, 2022. — 427 p. — (Methods in Molecular Biology 2405). — ISBN 978-1-0716-1854-7; ISBN 978-1-0716-1855-4. For over 35 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach...
New York: Humana Press, 2018. — 257 p. The study of human cancer stem cell (CSC) populations is fraught with technical challenges and seemingly insurmountable experimental tribulations, including limited and highly precious patient-derived samples, low cell numbers, optimal growth conditions, and difficulties in sorting and purifying heterogeneous cell populations derived from...
Springer, 2014. — 491 p. Mouse Genetics: Methods and Protocols provide selected mouse genetic techniques and their application in modeling varieties of human diseases. The chapters are mainly focused on the generation of different transgenic mice to accomplish the manipulation of genes of interest, tracing cell lineages and modeling human diseases. Composed in the highly...
Humana Press, 2018. — 281 p. — ISBN: 1493986961. —Series: Methods in Molecular Biology, № 1842 This second edition volume expands on the previous edition with updated discussions on new genetic, molecular, and cellular methods used to study somatic stem cells. The chapters in this book focus on the isolation, classification, purity, and plasticity of these stem cells in a...
Humana Press, 2018. — 281 p. — ISBN: 1493986961. —Series: Methods in Molecular Biology, № 1842 This second edition volume expands on the previous edition with updated discussions on new genetic, molecular, and cellular methods used to study somatic stem cells. The chapters in this book focus on the isolation, classification, purity, and plasticity of these stem cells in a...
Humana Press, 2018. — 239 p. — (Methods in Molecular Biology 1721). — ISBN 978-1-4939-7545-7. This volume presents various laboratory protocols, reviews, specific techniques and applications related to Schizosaccharomyces pombe. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the...
Humana Press, 2014. — 252 p. Series: Methods in Molecular Biology, № 1093 PIWI-interacting RNAs (piRNAs) are the third and most-recently discovered group of silencing-inducible small RNAs in animals. PIWI-Interacting RNAs: Methods and Protocols provides the most recent methods and protocols for studying piRNAs in the gonads of a wide range of species, as well as in any other...
Humana Press, 2018. - 230 p. - Volume 1831 of Methods in Molecular Biology. This volume looks at the latest techniques used to enable therapeutic molecules to be targeted for site-specific delivery. The chapters in this book discuss topics such as approaches to express biologically derived and synthetic nanocarriers; strategies to assist in nanocarrier targeting to specific cells...
New York: Humana Press, 2023. — 384 p. This detailed volume provides methods that can be used to study dendritic cell (DC) ontogeny, isolation, migration, and functions. After an introduction to murine and human DC subsets and their unique transcriptional, phenotypic, and functional properties, the book continues with sections covering in vivo studies, in vitro differentiation,...
2nd Edition. - Humana Press, 2018. - 455 p. - Volume 1727 of Methods in Molecular Biology. This volume collects protocols and procedures utilizing cellular, tissue, and whole animal models that can be applied to the investigation of neurotrophic factors and other agents impacting on these systems. It begins with chapters on the culture of neurons and glia from the central and...
New York: Humana Press, 2015. — 227 p. — (Methods in Molecular Biology 1299). This volume has been assembled for scientists interested in basic and applied biomedical research directed toward understanding the development, genetics and function of cardiomyocytes. The methods and protocols contained within it address cell culture techniques, cardiomyocyte differentiation and...
Humana, 2025. — 389 p. — ISBN-13 : 978-1071644294 This book seeks to provide an overview of traditional and emerging protocols used to examine the aetiology, mechanisms and pathophysiology of GVHD, as well as those used to identify novel biomarkers and to test existing and new therapies to prevent or treat GVHD. Written in the format of the highly successful Methods in...
Humana Press, 2005 — 497 p. E-ISBN: 1-59259-922-2 Series: Methods in Molecular Biology, № 308 With the recent completion of the sequencing of the human genome, it is widely anticipated that the number of potential new protein drugs and targets will escalate at an even greater rate than that observed in recent years. However, identification of a potential target is only part of...
Humana Press, 2017. — 429 p. — (Methods in Molecular Biology). — ISBN10: 1493965964. — ISBN13: 978-1493965960. This detailed volume gathers basic and advanced methods and protocols from in vitro assays and in vivo models to address the molecular and functional aspects of tau physiopathology. Divided into five parts that illustrate the underlying molecular mechanisms of Tau...
Humana Press, 1996. — 374 р. — (Methods in Molecular Biology 64). — ISBN: 0-89603-353-8. This book belongs to the well-known series Methods in Molecular Biology, mainly devoted to procedures and methodologies. It collects together protocols for the separation, purification and sequencing of peptides and proteins. It is composed of thirty short chapters written by specialists in...
Humana Press, 2014. — 391 p. Series: Methods in Molecular Biology, №1205 Yeast Genetics: Methods and Protocols is a collection of methods to best study and manipulate Saccharomyces cerevisiae, a truly genetic powerhouse. The simple nature of a single cell eukaryotic organism, the relative ease of manipulating its genome and the ability to interchangeably exist in both haploid and...
2nd edition. — Humana Press, 2016. — 565 p. — (Methods in Molecular Biology 1427). — ISBN 978-1-4939-3613-7. This second edition expands upon the previous volume with new and updated chapters. Auditory and Vestibular Research: Methods and Protocols, Second Edition guides readers through protocols on cell culture, tissue engineering, nanotechnology, high-throughput screening,...
Humana, 2022. — 413 p. This volume details methods on several aspects of circadian research. Chapters guide readers through the latest techniques and a wide variety of daily rhythmic processes, model organisms, circadian rhythms in the SCN and in peripheral organs, and describing in vitro systems and in silico methods. Written in the format of the highly successful Methods in...
Humana Press, 2001. — 272 p. — (Methods in Molecular Biology 156). — ISBN: 978-1-59259-062-9. The processing and presentation of antigens by major histocompatibility complex (MHC) molecules is a crucial immunological phenomenon in infectious disease, malignancy, autoimmune disease, and transplantation. In Antigen Processing and Presentation Protocols, well-recognized and...
Humana Press. 2011. 203 p. New and rapid advances in technology have equipped us with a variety of tools and platforms to ask fundamental questions of telomere regulation and have allowed investigators to carry out experiments using diverse model systems. For example, proteomic, genomic, and molecular approaches have afforded us unprecedented insight into the complex protein...
2nd edition. — Humana Press, 2021. — 352 p. — ISBN 978-1-0716-1475-4. This second edition provides new and updated chapters detailing recent advances in MYC research and current techniques. Chapters guide readers through protocols on how to express and purify MYC protein, X-ray crystallography, NMR, techniques to study how MYC is modified, apoptosis, senescence, proliferation,...
Humana Press, 2021. — 334 p. — (Methods in Molecular Biology, volume 2197). — ISBN: 978-1-0716-0871-5. This volume details practical procedures on the latest DNA vaccine technology. Chapters guide readers through methods and protocols on DNA vaccine design, the adjuvant influence, production and purification methodologies, delivery systems, and approaches of the influence of...
Humana Press, 2013. — 504 p. Series: Methods in Molecular Biology, № 950 For more than a century, microscopy has been a centerpiece of extraordinary discoveries in biology. Along the way, remarkable imaging tools have been developed allowing scientists to dissect the complexity of cellular processes at the nano length molecular scales. This volume presents a diverse collection...
2nd ed. — Humana, 2025. — 290 p. — ISBN-13 : 978-1071644454 This fully updated volume brings together synapse development methods that span from the macroscopic to the nanoscale in order to foster novel perspectives and encourage interdisciplinary integration. Beginning with a section on in vitro formation and manipulation of synapses, including cutting-edge approaches for...
3rd Edition. — Springer, 2020. — 447 p. — (Methods in Molecular Biology 2123). — ISBN 978-1-0716-0345-1. This third edition aims to provide new and updated methods on animal influenza viruses as well as more advanced protocols that will guide the reader in designing research. Chapters detail influenza in peridomestic animals, marine mammals, savian influenza, swine influenza,...
Humana Press, 2008. — 147 p. — (Methods in Molecular Biology 436). With the growing global fear of a major pandemic, avian influenza virus research has increased greatly in importance during this young century. In Avian Influenza Virus, an expert team of researchers and diagnosticians examine the fundamental yet essential virological methods for AI virus research and...
Humana Press, 2015. — 323 p. Series: Methods in Molecular Biology, № 1230 The book serves as a comprehensive guide to both key new techniques and established methods for the investigation of genetics, structural biology, transcription, and post-transcriptional events of opioid receptors. Other methods cover the cellular detection and trafficking of opioid receptors in vitro and in...
CRC Press LLC, 2003. — 218 р. — ISBN: 1584883278.
Although less than a decade old, the field of microarray data analysis is now thriving and growing at a remarkable pace. Biologists, geneticists, and computer scientists as well as statisticians all need an accessible, systematic treatment of the techniques used for analyzing the vast amounts of data generated by large-scale...
Humana Press, 2004 — 570 p. — E-ISBN: 1-59259-766-1 Series: Methods in Molecular Biology, № 268 Public Health Microbiology: Methods and Protocols is focused on microorganisms that can present a hazard to human health in the course of everyday life. There are chapters dealing with organisms that are directly pathogenic to humans, including bacteria, viruses, and fungi; on...
Humana Press, 2022. — 288 p. — (Methods in Molecular Biology 2548). — ISBN 978-1-0716-2580-4. This volume provides the most widely used protocols and techniques employed in lipopolysaccharide( LPS) transport studies. Chapters detail identification and analysis of essential genes and crucial mutations in LPS transport-related genes, LPS profile determination, LPS chemical...
Humana Press, 2014 — 245p. — (Methods in Molecular Biology 1112) — ISBN: 978-1-62703-773-0 (eBook), 978-1-62703-772-3 (Hardcover). Many fundamental discoveries concerning epigenetics and the elucidation of mechanisms of epigenetic regulation have developed from studies performed in plants. In Plant Epigenetics and Epigenomics: Methods and Protocols , leading scientists in the...
New York: Humana Press, 2014. — 307 p. — (Methods in Molecular Biology 1082). Providing widely used techniques in genetic model systems and many complementing animal models, the book focuses its expert contributions on two key technical aspects of developmental neurobiology: detection of gene expression and functional characterization of developmental control genes. Covering...
2nd edition. — Humana Press, 2020. — 520 p. — (Methods in Molecular Biology 2047). — ISBN 978-1-4939-9731-2. This book provides a thorough introduction to widely used techniques for the study of the intersection between developmental biology and neuroscience, an exceptional area to address and investigate impacting biological questions. The fully updated volume examines...
Humana Press, 2014. — 317 p. Series: Methods in Molecular Biology, №1083 In this volume, expert researchers in the field present the latest methods on quantitative analysis of plant metabolism. The methods focus on measurements, analyses and simulations of molecules, fluxes, and ultimately entire metabolic pathways and networks. Written in the highly successful Methods in...
Humana Press, 2014. — 375 p. Series: Methods in Molecular Biology, № 1158 The book provides a collection of protocols to investigate clock-controlled parameters including transcript and small RNA levels, promoter activity using luciferase reporters, protein levels and posttranslational modification, protein-protein interaction, in vivo DNA-protein interaction and RNA-protein...
New York: Humana, 2022. — 383 p. This volume details cutting-edge methods and protocols for the development, characterization, and applications of multienzyme assemblies. Chapters guide readers through up-to-date techniques applied for the development and emerging applications of multi-enzymatic systems for biotransformations, biosensing, molecular-scale diagnostics and...
Humana Press, 2016. — 429 p. In Genomics Protocols , 2nd Edition, a team of expert researchers share the most current information in a field that has recently switched emphasis from gene identification to functional genomics and the characterization of genes and gene products. This volume approaches its complex subject with a broad perspective to supply its reader with a vital...
Humana Press, 2019. — 216 p. — (Methods in Molecular Biology 1907). This book presents protocols for identification of genetic drivers of cancer. Chapters guide readers through a brief history of cancer gene discovery, in silico approaches, in vitro approaches, and in vivo approaches using forward genetic screens in mice. Written in the highly successful Methods in Molecular...
Humana Press, 2015 — 439p. — (Methods in Molecular Biology 1308) — ISBN: 978-1-4939-2684-8 (eBook), 978-1-4939-2683-1 (Hardcover).
This volume provides a fundamental overview of the current state of the art in natural products from marine algae, linking the complex and diverse natural resource with recent developments in extraction, analytical and bioactivity testing...
Humana Press, 2011. — 485 p. Recent outbreaks of swine influenza and avian influenza, along with the remaining and in some cases expanding threats from HIV, dengue virus, and the viruses causing hepatitis, have reinforced the need for rapid, accurate and cost-effective diagnosis of viral disease. Diagnostic Virology Protocols, Second Edition brings the field fully up-to-date...
Humana Press, 2017. — 365 p. — (Methods in Molecular Biology 1506). This volume features up-to-date protocols for the isolation, preservation, and validation of various cell sources comprising large and small animal models, examining the impact of cell transplantation on acute and chronic liver diseases. Hepatocyte Transplantation: Methods and Protocols guides readers through...
2nd edition. — Humana Press, 2020. — 673 p. — (Methods in Molecular Biology 2076) — ISBN: 978-1-4939-9882-1. This second edition volume expands on the previous editions with updates on the commonly used techniques and analysis used by clinicians and scientists to study diabetes. The chapters in this book cover topics such as nutritional models of type 2 diabetes mellitus; the...
2nd edition. — Humana Press, 2020. — 300 p. — (Methods in Molecular Biology 2076) — ISBN: 978-1-4939-9880-7. This second edition volume expands on the previous editions with updates on the commonly used techniques and analysis used by clinicians and scientists to study diabetes. The chapters in this book cover topics such as nutritional models of type 2 diabetes mellitus; the...
Humana Press, 2014. — 365 p. — (Methods in Molecular Biology 1094). In Functional Analysis of DNA and Chromatin, expert researchers in the field provide an overview of standard and more recent methods for the functional analysis of the genetic material. These include methods on DNA-dye binding modes, chromatin staining, nuclear dispersion assays, DNA labeling in vivo, sister...
Humana Press, 2011. — 253 p. — (Methods in Molecular Biology 740). — ISBN 978-1-61779-107-9. Whether the question is one of basic cell survival, or whether it is being used to correlate cell number to some other factor such as matrix synthesis, an estimate of cell viability is universally required. In Mammalian Cell Viability: Methods and Protocols, experts in the field...
Humana, 2022. — 355 p. This detailed volume features techniques to explore the complex interface that separates the systemic circulation from the central nervous system, known as the blood-brain barrier (BBB). Beginning with an introduction to its physiology, the book continues with sections on using pluripotent stem cells in models of the BBB, co-culture, permeability and...
Humana Press, 2014 — 463p. — (Methods in Molecular Biology 1114) — ISBN: 978-1-62703-761-7 (eBook), 978-1-62703-760-0 (Hardcover). Gene correction is a technology that gives us the tools for both repairing and mutating DNA, for discovering gene functions and for engineering new genetic variants. Gene Correction: Methods and Protocols provides a user friendly, detailed and...
New York: Humana Press, 2015. — 488 p. The book is the first one solely dedicated to methodological approaches designed to study galectin function. The galectin family represents one of the most pleiotropic families, with individual members having been implicated in various aspects of nearly every biological process described, from RNA splicing to complex regulatory circuits that...
New York: Humana Press, 2016. — 214 p. — (Methods in Molecular Biology 1379). — ISBN: 978-1-4939-3190-3, 978-1-4939-3191-0. Despite the advent of personalized therapies, cancer still remains a leading cause of death worldwide. The number of cancer cases is rising yearly and is expected to double in the next 20 years. The largest increase of new cases will occur mainly in lower...
2nd ed. — Humana Pres, 2016. — 310 p. — ISBN: 1493966898. Series: Methods in Molecular Biology, No 1539. This second edition explores up-to-date tools in various function-based technologies currently used in metagenomics. The chapters in this book discuss all of the working steps involved in these technologies, such as: DNA isolation from soils and marine samples followed by...
New York: Humana Press, 2000. — 341 p. A comprehensive protocol book featuring cutting-edge methods and techniques to study molecular, cellular, biochemical, and biophysical aspects of the extracellular matrix Bench-tested in-vivo, ex-vivo and in-vitro methods written by experts who provided detailed step-by-step instructions and trouble-shooting Easy-to-follow techniques that...
Humana Press, 2009. — 352 p. Series: Methods in Molecular Biology, vol.554 Since the publication of the first edition of Mitochondrial DNA: Methods and Protocols in 2002, the number of unique heritable mtDNA mutations recognized as being associated with bioenergetic dysfunction, cell death, and disease has grown apace. At the same time, our understanding of the basic biology of...
Springer, 2012. — 206 p. — (Methods in Molecular Biology, №862).
Molecular cloning and DNA-based analysis have become part of every molecular life science laboratory. The rapid adoption of DNA-based techniques has been facilitated by the introduction of the polymerase chain reaction (PCR), which has made cloning and characterization of DNA quick and relatively simple. PCR is...
Humana Press, 2013. — 431 p. — (Methods in Molecular Biology 940). Biolistic transfection represents a direct physical gene transfer approach in which nucleic acids are precipitated on biologically inert high-density microparticles (usually gold or tungsten) and delivered directly through cell walls and/or membranes into the nucleus of target cells by high-velocity acceleration...
Humana Press, 2024. — 248 p. — (Methods in Molecular Biology 2720). — ISBN 978-1-0716-3468-4. This volume covers the latest key aspects of therapeutic protein applications. Chapters in this book cover topics such as the discovery, production, and conjugation of protein-proteins with discussions on the direction of future development and advancements; ways to use these...
Humana Press, 2014. — 269 p. Series: Methods in Molecular Biology, № 1151 The book focuses on laboratory procedures used in recent efforts for constructing synthetic multicellular systems and their applications. In particular, constructing multicellular systems to form various microbial ecosystems has been extensively explored to examine evolution and interactions of microbial...
Humana Press, 2016. — 226 p. — (Methods in Molecular Biology 1367). — ISBN: 978-1-4939-3130-9 (eBook), 978-1-4939-3129-3 (Hardcover). This volume details methods and protocols for the synthesis and characterization of glycopolymers and their biomedical applications. Chapters are divided into three parts covering synthesis and characterization of glycopolymers;...
Humana, 2022. — 416 p. This detailed collection explores recent advances in molecular imaging techniques involving bioluminescence, currently employed in biolaboratories around the world. Volume 2 delves into techniques for heterogeneous conjugates, protein fragment-complementation assays, BRET-based imaging, as well as instrumentation and software. Written for the highly...
Humana Press, 2010. — 386 р. — (Methods in Molecular Biology 639). — ISBN: 978-1-60761-701-3. A number of abiotic factors such as drought, salinity, extreme temperatures (very low and very high), low or high light intensity, deficiency or toxic levels of nutrients have huge impacts on crop productivity. In the last few decades, we are witnessing tremendous efforts to understand...
New York: Humana Press, 2012. — 334 p. Protein–protein interactions (PPIs) are strongly predictive of functional relationships among proteins in virtually all processes that take place in the living cell. Therefore, the comprehensive exploration of interactome networks is one of the major goals in systems biology. The aim of Two Hybrid Technologies: Methods and Protocols is to...
Humana Press, 1996. — 319 p. — ISBN: 0896033589, 9780896033580
Series: Methods in molecular biology, N. 070.
Computers have revolutionized the analysis of sequencing data. It is unlikely that any sequencing projects have been performed in the last few years without the aid of computers. Recently their role has taken a further major step forward. Computers have become smaller...
Humana Press, 2013. — 569 p. Series: Methods in Molecular Biology, № 931 Cell Imaging is rapidly evolving as new technologies and new imaging advances continue to be introduced. In the second edition of this book expands upon the previous editions with current techniques that includes confocal microscopy, transmission electron microscopy, atomic force microscopy, and laser...
Humana Press, 2009. — 331 р. — (Methods in Molecular Biology 520). Biomarkers are molecular indicators of a biological status and, as biochemical species, can be interrogated to evaluate disease status and therapeutic interventions. Biomarkers may be detectable in the blood, other body fluids, or tissues. The expectation is that the level of an informative biomarker is related to...
New York: Humana Press, 2022. — 424 p. This second edition volume expands on the previous edition with updated methodologies and practical tips to overcome obstacles associated with experimentation pertaining to chemistry, biology, physiology, pathology, medical and dental sciences, and pharmacology of CCN proteins. The chapters in this book cover topics such as CCN4...
New York: Humana Press, 2017. — 532 p. This detailed volume describes cutting-edge techniques in three distinct and complementary areas of contemporary kinase biology research. Beginning with a section on synthetic biology, chemical biology, and screening approaches to kinase signaling networks, the book continues with sections on mass spectrometry and metabolic analysis of...
ITexLi, 2018. — 469 p. — ISBN 1789236754 9781789236750 1789236746 9781789236743 1838812725 9781838812720. This book presents up-to-date knowledge about mitochondrial disease and its complex relation to some diseases such as cardiac failure, cancer, and Alzheimer's and Parkinson's diseases. This book will be essential for readers who are interested in life sciences, especially...
Humana Press, 2017. — 337 p. — (Methods in Molecular Biology 1511). This book brings together the major techniques used in the isolation or enrichment of individual populations of organelles and other subcellular structures from plants with the goal that, by being able to isolate subcellular structures, the research and understanding of various facets of compartmentalized...
Humana Press, 2017. — 496 p. — (Methods in Molecular Biology 1493). This volume arranged into three sections describes biochemical, in vitro, and in vivo protocols on Semaphorins. Chapters focus on approaches that would allow the novice to study Semaphorins and employ robust assays to characterize mechanisms of action. Written in the highly successful Methods in Molecular Biology...
Humana Press, 2024. — 266 p. — (Methods in Molecular Biology 2711). — ISBN 978-1-0716-3428-8. This volume focuses on the latest research methods and techniques used by researchers to study vascular permeability/hyperpermeability in a science laboratory setting. The chapters in this book cover topics such as determination of solute permeability of microvascular endothelial cell...
Humana Press, 2018. — 285 p. — (Methods in Molecular Biology 1738). — ISBN 978-1-4939-7642-3. This volume explores the different approaches and techniques used by researchers to study the recent challenges and developments in metabolic profiling. This book is divided into IV parts. Part I contains chapters that highlight basic concepts, such as experimental design, data...
Humana, 2002. — 214 p. Bimal D. Theophilus and Ralph Rapley provide biological and clinical investigators with a comprehensive collection of new, recent, and updated PCR-based screening methods suitable for detecting the presence of both known and novel mutations. The methods cover point mutations (e.g., ASO-PCR, SSCP, DGGE, chemical cleavage), deletions (multiplex PCR, FISH,...
2nd edition. — Humana Press, 2022. — 701 p. — (Methods in Molecular Biology 2410). — ISBN 978-1-0716-1883-7. This volume provides a practical guide providing step-by-step protocol to design and develop vaccines for human diseases. Divided into three volumes, Volume 1: Vaccines for Human Diseases guides readers through an introductory section on future challenges for...
2nd edition. — Humana Press, 2022. — 535 p. — (Methods in Molecular Biology 2412). — ISBN 978-1-0716-1891-2. This volume provides a practical guide providing step-by-step methods and protocols on vaccine development and production. Divided into three volumes, Volume 3: Resources for Vaccine Development guides readers through chapters on vaccine adjuvants, vaccine vectors,...
2nd edition. — Humana Press, 2022. — 360 p. — (Methods in Molecular Biology 2411). — ISBN 978-1-0716-1887-5. This volume provides a practical guide providing step-by-step protocol to explore vaccines for farm and companion animals, as well as for fish and insects. Divided into three volumes, Volume 2: Vaccines for Veterinary Diseases guides readers through veterinary vaccines,...
Humana Press, 2019. — 730 p. — (Methods in Molecular Biology 2055) — ISBN: 978-1-4939-9772-5 This book provides the immune oncology (IO) community with a deeper understanding of the scope of the biomarker methods to potentially improve the outcome from immunotherapy. The editors secured the input from experts in the field dedicated to translating scientific research from bench...
Humana Press, 2014. — 711 p. Series: Methods in Molecular Biology, №1102 In this volume, expert researchers and clinicians in the field of melanoma provide updated information on biomarkers and assays for diagnosis, prognosis, and assays predicting response to treatment for routine testing. The focus of the volume is on biomarkers with established clinical validity rather than...
Humana, 2016. — 425 p. This second edition expands on the first edition with new chapters describing methods for studying cell movement, molecular components involved in chemotaxis, spatiotemporal dynamics of signaling components, and quantitative modeling, as well as several updated chapters from the first edition. Various methods to investigate directional cell growth and...
Humana Press, 2017. — 317 p. — (Methods in Molecular Biology 1551). This volume details protocols for genetic, molecular, cytological, and bioinformatic methods for determining haplotypes. Haplotyping: Methods and Protocols guides readers through methods that directly type haploid cells, difficult-to-resolve gene families, high-resolution, short range haplotyping for targeted...
Humana Press, 2017. — 420 p. — (Methods in Molecular Biology 1575). This detailed volume presents a set of protocols useful for researchers in the field of recombinant immunoglobulin and alternative scaffold engineering, aptamer development, and generation of molecularly imprinted polymers (MIPs). Part I includes methods that deal with amino-acid based synthetic antibodies....
New York: Humana Press, 2018. — 191 p. This volume explores the latest techniques and approaches used to study this emerging form of cell death. The chapters in this book cover topics such as distinguishing necroptosis from apoptosis; detecting MLKL oligomerization during programmed necrosis; analyzing the RIPK3 necrosome complex; using RIPK1 kinase small molecule inhibitors in...
3rd Edition. — Springer, 2020. — 410 p. — (Methods in Molecular Biology 2131). — ISBN: 978-1-0716-0388-8. This book covers a wide range of diverse immunoinformatics research topics, involving tools and databases of potential epitope prediction, HLA gene analysis, MHC characterizing, in silico vaccine design, mathematical modeling of host-pathogen interactions, and network...
Humana Press, 2022. — 972 p. — (Methods in Molecular Biology 2497). — ISBN 978-1-0716-2309-1. This volume details comprehensive protocols and methodologies to assess mitochondrial bioenergetics and dynamics in different tissues and cells involving health and pathological states. Chapters guide readers through methods for assessment of the energy metabolism including Oxygen...
Humana Press, 2022. — 432 p. — (Methods in Molecular Biology 2497). — ISBN 978-1-0716-2308-4. This volume details comprehensive protocols and methodologies to assess mitochondrial bioenergetics and dynamics in different tissues and cells involving health and pathological states. Chapters guide readers through methods for assessment of the energy metabolism including Oxygen...
Humana Press, 2011. - 337 p. - Volume 790 of Methods in Molecular Biology. As two relatively new fields of study, proteomics and nanotechnology have developed in parallel with each other to allow an increased precision in the identification of post-translational protein modifications as well as to provide a more automated isolation and detection of rare proteins in both serum...
Springer, 2020. — 469 p. – (Methods in Molecular Biology 2071). — ISBN: 978-1-4939-9856-2. This volume covers a diverse collection of protocols currently being used by the Toxoplasma research community, and also looks at innovative methods that are pushing the boundaries of possibilities. Chapters in this book discuss topics such as isolation and genotyping of Toxoplasma gondii...
Humana Press, 2024. — 244 p. — (Methods in Molecular Biology 2727). — ISBN 978-1-0716-3490-5. This detailed volume explores methods currently used to investigate the cell wall of various bacterial species and pathogens. By using a combination of genetic, molecular, biochemical, and cytological techniques, the protocols address many fundamental questions involving the...
Humana Press, 2017. — 434 p. — (Methods in Molecular Biology 1527). This volume provides comprehensive protocols that researchers can use to dissect out fundamental principles and molecular mechanisms of hypertension, extending from genetics of experimental hypertension to biomarkers in clinical hypertension. Hypertension: Methods and Protocols guides readers through chapters on...
Humana Press, 2014 — 425p. — (Methods in Molecular Biology 1120) — ISBN: 978-1-62703-791-4 (eBook), 978-1-62703-790-7 (Hardcover).
Featuring experimental approaches that shed light on the complexity of Ras GTPase biological functions, Ras Signaling: Methods and Protocols contains general overviews and detailed applications of both well-established and recently developed...
Humana, 2022. — 642 p. — (Methods in Molecular Biology 2491). — ISBN-13 9781071622841. Поверхностный дисплей This detailed volume explores a wide variety of applications of yeast surface display, an extensively used protein engineering technology. Beginning with detailed protocols for the construction and efficient selection/screening of yeast surface display libraries, as well...
Humana Press, 2016. — 261 p. — (Methods in Molecular Biology 1442). — ISBN: 978-1-4939-3687-8 This volume summarizes current techniques; ranging from protocols for virus growth, isolation, quantification and generation of recombinant RSV virus to procedures for the efficient characterization of the host immune response to RSV infection. These techniques are used in numerous...
Humana, 2022. — 402 p. This volume details the history of Retinitis Pigmentosa and current treatment options. Chapters guide readers through CRISPR, gene therapy, stem cell therapy, next-generation sequencing methods, gene editing, and translational applications of other therapies to the treatment of Retinitis Pigmentosa. Written in the successful Methods in Molecular Biology...
Humana, 2023. — 427 p. This volume provides detailed protocols for the isolation, enumeration, characterization of diverse bacteriophages, including both small and jumbo bacteriophages, from soil, fecal, municipal wastewater, and from food niche samples. Chapters highlight the diversity of bacteriophages in different environments, quantifications using culture and molecular...
Humana Press, 2016 — 3rd Ed. — 449p. — (Methods in Molecular Biology 1341) — ISBN: 978-1-4939-2954-2 (eBook), 978-1-4939-2953-5 (Hardcover). This extensive volume explores areas of intense activity related to the very early commitment of stem cells to particular lineages and the progression of differentiation to mature cell stages. Research on embryonic stem cells continues to...
Humana Press, 2005. — 499 p. Series: Methods in Molecular Biology, № 289 In recent years, our ability to understand and manipulate epidermal cells has increased tremendously, opening significant new possibilities in both basic science research and in regenerative medicine, including would healing and transplantation. This volume covers different methods and models for culturing...
Humana Press, 2013. — 320 p. Series: Methods in Molecular Biology, № 989 During the last decade, an increased interest in somatic stem cells has led to a flurry of research on one of the most accessible tissues of the body: skin. Much effort has focused on such topics as understanding the heterogeneity of stem cell pools within the epidermis and dermis, and their comparative...
Humana Press, 2015 — 210p. — (Methods in Molecular Biology 1212) — ISBN: 978-1-4939-2590-2 (eBook), 978-1-4939-2589-6 (Hardcover).
This volume examines cell-cell interactions and stem cell renewal, two topics that are now inexorably linked as science strives to understand the stem cell niche and its function. Gathering a number of representative protocols, this detailed...
2nd Edition. — Humana Press, 2019. — 353 p. — (Methods in Molecular Biology 2045). — ISBN: 978-1-4939-9712-1. This fully updated book collects protocols that reflect current investigations into how stem cell populations change during organismal aging. These methodologies are well-established and described in easy to follow fashion so as to be valuable for not only experts but...
Humana Press, 2013. — 180 p. Series: Methods in Molecular Biology, № 976 Over time, it has become clear that changes in stem cells do occur during aging, not only in their number but also in their relationship to their microenvironment and their functionality as reflected in changes to their metabolome. This volume brings together chapters from expert contributors with...
Humana Press, 2015. — 220 p. — (Methods in Molecular Biology 1283). — ISBN: 978-1-4939-2435-6 (eBook), 978-1-4939-2434-9 (Hardcover). This volume collects a series of protocols describing the kinds of infrastructures, training, and standard operating procedures currently available to actualize the potential of stem cells for regenerative therapies. Stem Cells and Good...
Humana Press, 2002. - 516 p. Series: Methods in Molecular Biology, №185 The book could be used effectively by experienced or novice researchers in the field since the protocols are written clearly and concisely. There are 35 brief chapters in this book by over 80 authors who are experienced and knowledgeable researchers in the field.Among the many experimental protocols...
Humana, 2022. — 278 p. This new edition brings together some of the latest developments and protocols reflecting the rapidity with which bioreactor technologies are advancing and being applied. Given that the use of bioreactors in cell biology is becoming more commonplace as attempts are made to scale-up production of various types of cells for regenerative medicine and...
Humana Press, 2016 — 496p. — (Methods in Molecular Biology 1357) — ISBN: 978-1-4939-3055-5 (eBook), 978-1-4939-3054-8 (Hardcover). This volume aims to be a collection of essential protocols in iPS cell field. Induced Pluripotent Stem (iPS) Cells: Methods and Protocols guides readers through multiple facets of stem cell biology, lineage commitment and differentiation. Written...
2nd edition. — Humana Press, 2022 — 829 p. — (Methods in Molecular Biology 2454). — ISBN 978-1-0716-2118-9. This extensive new edition presents protocols reflecting the great strides made in the study of induced pluripotent stem (iPS) cells. The collection explores new and improved methods for the generation, expansion, and maintenance of iPS cells from different tissue types,...
Humana Press, 2015 — 443 p. — (Methods in Molecular Biology 1212) — ISBN: 978-1-4939-2590-2 This volume examines cell-cell interactions and stem cell renewal, two topics that are now inexorably linked as science strives to understand the stem cell niche and its function. Gathering a number of representative protocols, this detailed collection promises to provide readers with...
Humana, 2016. — 295 p. This volume provides protocols and procedures for determining and modeling RNA structure. Chapters guide the reader through protocols for RNA secondary structure prediction, single sequence modeling, Crumple, RNAstructure to model conserved secondary structures with multiple homologs, the prediction of bimolecular secondary structures with RNAstructure,...
ITexLi, 2022. — 110 p. — ISBN 1839693533 9781839693533 1839693525 9781839693526 1839693541 9781839693540. The book explores distinct aspects of protein purification and characterization steps. It discusses solubility problems, resin selection tricks, and essential credentials in a purification process. The book examines aggregation and proteinopathy-related protein detection...
ITexLi, 2021. — 264 p. — ISBN 1839692553 9781839692550 183969257X 9781839692574. This book discusses parasite biology, antimalarial drugs and their mechanism of action, and the dynamic situation of evolving drug resistance of parasites, which has become a pressing issue. The book provides insight into the plasmodium species, the role of cytokines in activating immune response...
Humana Press, 2000. — 318 p. Series: Methods in Molecular Biology, № 130 In Transcription Factor Protocols, Martin Tymms has created a powerful compendium of the major techniques for the study of those DNA sequences and protein factors that regulate the transcription of protein encoding genes. With chapters contributed by leading investigators well versed in the methods, this...
Humana Press, 1994. - 405 p. ISBN: 0896032884 Series: Methods in Molecular Biology, №37 This book provides molecular biology laboratories with the most powerful techniques for employing in vitro transcription and translation systems. Detailed experimental protocols are provided for prokaryotic transcription and translation systems, along with protocols for the many techniques...
New York: Humana Press, 2023. — 449 p. This volume covers a wide range of approaches utilized to decipher cellular and molecular mechanisms that contribute to successful nerve regeneration leading to functional recovery. Chapters detail a variety of models utilizing both in vivo and in vitro approaches, physical injury models, methods for the isolation/analysis of various...
Humana, 2023. — 422 p. This volume provides the latest methods for synthesis, structural analysis, and elucidation of the mechanism. Chapters guide readers through methods on protein cages for nanotechnology, analyze designed protein cages, determine the structures, and even perform theoretical analysis. Written in the format of the highly successful Methods in Molecular...
Humana Press, 2020. — 271 p. — (Methods in Molecular Biology 2161). This volume focuses on RNAs interacting with chromatin and their function. Chapters guide readers through transcription, splicing, non-coding RNA function and manipulation of gene expression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their...
Humana Press, 2023. — 160 p. — (Methods in Molecular Biology 2687). — ISBN 978-1-0716-3306-9. This volumes presents current methods used in the research of schizophrenia. Chapters guide readers through molecular techniques, in vivo approaches, cell cultures and heterologous expression systems, and even research methods involving human studies. Written in the highly successful...
Humana Press, 2017. — 264 p. — (Methods in Molecular Biology 1518). Small molecule microarrays (SMM) were introduced in 1999 and within just a short span of time, have established themselves as a vibrant next generation platform for high-throughput screening. Small Molecule Microarrays: Methods and Protocols consolidates a significant collection of examples, which serve to guide...
Humana Press, 2015. — 295 p. Series: Methods in Molecular Biology, № 1236 The book addresses recent developments in genome analyses and cytological technologies being used today to learn more about plant virology. Opening with chapters covering techniques relevant to the detection of unknown viruses and disease diagnosis, this detailed volume continues with chapters on the...
Humana Press, 2022. — 568 p. — (Methods in Molecular Biology 2431). — ISBN 978-1-0716-1989-6. This volume covers a wide range of model systems from invertebrate to humans and in vitro and in vivo. Chapters detail methods on squid, aplysia, xenopus, mouse in vivo, ex vivo, rodent primary neurons, human-derived neurons, zebrafish, drosophila in vivo, primary neurons, c. elegans,...
Humana Press, 2012. - 362 p. - Membrane proteins play key roles in numerous cellular processes, in particular mediating cell-to-cell communication and signaling events that lead to a multitude of biological effects. Membrane proteins have also been implicated in many critical diseases such as atherosclerosis, hypertension, diabetes and cancer. In Membrane Protein Structure...
Humana Press, 2023. — 316 p. — (Methods in Molecular Biology 2668). — ISBN 978-1-0716-3202-4. This detailed volume presents hands-on technological protocols used to target an array of cell-secreted extracellular vesicles (EVs) in a variety of biological systems. Beginning with methods for EV purification and analysis, the book continues with sections on the study of EV...
Humana, 2024. — 298 p. This volume looks at the latest advancements used by researchers to study psychophysiology and cognitive neurosciences. The chapters in this book cover topics such as classical event-related brain responses (P300, MMN, and CNV); anatomical structures and physiological mechanisms underlying the capability of feeling pain and smelling;...
New York: Humana, 2023. — 351 p. This volume detials diverse methodological approaches on the assembly and applications of DNA origami assemblies. Chapters guide readers through different synthetic and computational methods, isolation and structural characterization of 2D and 3D DNA origami nanoarchitectures, nanophotonics, drug delivery, biophysics, and synthetic...
Humana Press, 2023. — 777 p. — (Methods in Molecular Biology 2639). — ISBN 978-1-0716-3028-0. This volume detials diverse methodological approaches on the assembly and applications of DNA origami assemblies. Chapters guide readers through different synthetic and computational methods, isolation and structural characterization of 2D and 3D DNA origami nanoarchitectures,...
Humana Press, 2014 — 303p. — (Methods in Molecular Biology 1116) — ISBN: 978-1-62703-764-8 (eBook), 978-1-62703-763-1 (Hardcover). In DNA Cloning and Assembly Methods, expert researchers in the field detail many of the methods which are now commonly used for DNA cloning and make cloning procedures faster, more reliable and also suitable for high-throughput handling. These...
Humana Press, 2017. — 337 p. — (Methods in Molecular Biology). — ISBN10: 1493966561. — ISBN13: 978-1493966561. This volume introduces databases containing the results from the recent revolution in sequencing technologies. Chapters in Plant Genomics Databases: Methods and Protocols describe database content, as well as typical use-cases. Some chapters explore databases that...
Humana Press, 2014. — 383 p. Series: Methods in Molecular Biology, № 1172 Cytokines are pleiotropic regulatory proteins involved in essentially all biological processes and associated with a wide variety of diseases, including inflammatory disorders as well as many types of cancer and leukemia. Knowledge about the quantitative and qualitative nature of cytokine production is...
Humana Press, 2022. — 384 p. — (Methods in Molecular Biology 2385). — ISBN 978-1-0716-1766-3. This detailed book provides an overview of various classes of computational techniques, including machine learning techniques, commonly used for evaluating kinetic parameters of biological systems. Focusing on three distinct situations, the volume covers the prediction of the kinetics...
Humana Press, 2015. — 305 p. — (Methods in Molecular Biology 1325).
This volume focuses on malaria vaccinology, and introduces researchers to a complete overview of the protocols and tools used by molecular and cellular malariologists. The chapters within this book talk about interventions concerning all aspects of life cycle progression--measuring antibody responses to blood...
Humana, 2024. — 254 p. — ISBN-13 : 978-1071639337 This volume explores the latest advancements made in the field of mass spectrometry (MS) based single cell proteomics. The chapters in this book focus on technologies, methods, and biological applications and together cover the entire single cell proteomics analysis workflow, from sample collection and preparation through liquid...
Humana Press, 2011. — 552 p. — (Methods in Molecular Biology 698). — ISBN: 978-1-60761-998-7. Mesenchymal Stem Cells have seen an unprecedented level of interest in the last decade, primarily due to their relative ease of isolation, the large numbers of cells present in the adult, and the ability to propagate these cells in culture. In Mesenchymal Stem Cell Assays and...
New York: Humana Press, 2015. — 307 p. — (Methods in Molecular Biology 1300). Since the discovery of DNA structure and throughout the ensuing "DNA era," the field of DNA replication has expanded to cover a vast number of experimental systems. In DNA Replication: Methods and Protocols, expert researchers present a collection of techniques and approaches used to investigate DNA...
Humana Press, Springer Science+Business Media, LLC, 2009. — 497 p. — (Methods in Molecular Biology 471) — ISBN: 9781588299781. This two-volume set compiles areas of research that cover etiological factors or determinants that contribute in the development of cancer as well as describe the latest technologies in cancer epidemiology. Emphasis is placed on translating clinical...
Humana Press, Springer Science+Business Media, LLC, 2009. — 481 p. — (Methods in Molecular Biology 472) — ISBN: 9781603274913. This two-volume set compiles areas of research that cover etiological factors or determinants that contribute in the development of cancer as well as describe the latest technologies in cancer epidemiology. Emphasis is placed on translating clinical...
Humana Press, 2019. - 160 p. - Volume 1930 of Methods in Molecular Biology. This volume discusses the latest developments in cellular, molecular, biochemical, and imaging assays to study the biology and functions of T-cells. The chapters in this book cover topics such as LFA-1/ICAM-1 interactions in T-cell motility; using 3D-SIM to dissect signaling cross-talks in motile T-cells;...
Humana Press, 2015 — 271p. — (Methods in Molecular Biology 1330) — ISBN: 978-1-4939-2848-4 (eBook), 978-1-4939-2847-7 (Hardcover). This volume provides an understanding of the factors involved in nuclear reprogramming, which is essential for the success of reprogramming. The book is aimed at reprogramming differentiated cells and germ line transmission of pluripotent stem cells...
Humana Press, 2015. — 298 р.
Series Methods in Molecular Biology, №1251
This volume provides an overview of advanced fluorescence microscopy, covering a broad range of methods. Each chapter focuses on a different method and provides a practical guide for application in biological systems. Written in the highly successful Methods in Molecular Biology series format, chapters...
Humana Press, 2014. — 353 p. Series: Methods in Molecular Biology, № 1087 The book collects key experimental protocols that have provided the basis of the major discoveries of the field. Split into five sections, this detailed volume covers mapping of the HIV life cycle, isolation, co-receptor use, and cell tropism of HIV-1, in vivo quantification of HIV-1, biological aspects of...
Humana Press, 2020. — 514 p. — (Methods in Molecular Biology 2099). — ISBN 978-1-0716-0211-9. This volume provides various techniques and methodologies currently used in the study of MERS-CoV. Chapters are divided into four parts detailing evolution and entry of MERS-coronavirus, genetic alteration and structural determination of MERS-coronavirus proteins, quantitation of virus...
Humana Press, 2018. - 247 p. - Volume 1689 of Methods in Molecular Biology. This up-to-date volume includes protocols that illustrate the broad use of chromatin immunoprecipitation (ChIP) and ChIP-related methods in a variety of biological research areas. The collection also includes protocols designed to improve the performance of ChIP for specific applications. Written in the...
Humana Press, 2013. — 264 p. Series: Methods in Molecular Biology, № 1005 The use of proteomics to study complex diseases such as cardiovascular disease, the leading cause of death in developed countries, has grown exponentially in recent years. Proteomics is a rapidly expanding investigation platform in cardiovascular medicine and is becoming integrated and incorporated into...
Humana Press, 2013. — 243 p. — (Methods in Molecular Biology 1000). Proteomics is a rapidly expanding investigation platform in cardiovascular medicine. Driven by major improvements in mass spectrometry (MS) instrumentation and data analysis, the proteomics field has flourished in recent years particularly in the study of complex diseases. These recent advances are...
2nd edition. — Humana Press, 2022. — 353 p. — (Methods in Molecular Biology 2471). — ISBN 978-1-0716-2192-9. This second edition provides an overview of recent developments and approaches used by researchers to investigate the properties and functions of mammary epithelial and stem cells, which will contribute to understand the heterogeneity of the mammary gland and of breast...
Humana Press, 2021. — 361 p. — ISBN 978-1-0716-1739-7. This volume details methods of identifying synthetic lethal, genetic interactions by various approaches in different model systems including human cancer cells. Chapters guide readers through genetic interactions in model organisms, RNA interference, CRISPR/Cas9 based genome editing technologies, drug-gene interactions,...
Humana Press, 2021. — 361 p. — ISBN 978-1-0716-1739-7. This volume details methods of identifying synthetic lethal, genetic interactions by various approaches in different model systems including human cancer cells. Chapters guide readers through genetic interactions in model organisms, RNA interference, CRISPR/Cas9 based genome editing technologies, drug-gene interactions,...
ITexLi, 2021. — 127 p. — ISBN 1839626860 9781839626869 1839628162 9781839628160. This book evaluates and comprehensively summarizes the scientific findings that have been achieved through RNA-sequencing (RNA-Seq) technology. RNA-Seq transcriptome profiling of healthy and diseased tissues allows FOR understanding the alterations in cellular phenotypes through the expression of...
New York: Humana Press, 2002. — 435 p. Calcium-binding proteins play an important role in a variety of vital biological processes, ranging from blood clotting and signal transduction in cells, to attaching proteins to membranes and serving as an integral source of calcium. In Calcium-Binding Protocols- Volume 1: Reviews and Case Histories and Volume 2: Methods and...
Humana Press, 2013. — 373 p. Series: Methods in Molecular Biology, № 984 Capillary electrophoresis (CE) is a relatively new separation technique suitable for handling small amounts of sample very important in bioanalytical research and in various clinical, diagnostic, genetic, and forensic applications. In this volume, expert researchers in the field provide key techniques to...
Humana Press, 2004. — 272 p. Series: Methods in Molecular Biology, №262 Determination of Mitotic Recombination Rates by Fluctuation Analysis in Saccharomyces cerevisiae Determination of Intrachromosomal Recombination Rates in Cultured Mammalian Cells Intrachromosomal Homologous Recombination in Arabidopsis thaliana Analysis of Recombinational Repair of DNA Double-Strand Breaks...
Humana Press, 2014. — 343 p. Series: Methods in Molecular Biology, № 1086 Focusing on in vitro and intracellular RNA structure formation, this volume provides a comprehensive collection of experimental protocols which are suitable to dissect RNA folding pathways and to characterize the structure of RNA folding intermediates at nucleotide or even atomic resolution. The presented...
MMB vol.004, Humana Press, 1988, ISBN: 0896031276 Electrophoresis of RNA Denatured with Glyoxal or Formaldehyde Computer Analysis of DNA Sequences Detection of Sequence-Specific Protein-DNA Interactions by the DNA-Footprinting Technique Dligonucleotide Synthesis Using the Manual Phosphotriester Method
Humana Press, 1988. - 524 p. ISBN: 0896031268 Series: Methods in Molecular Biology, №3 Prevention of Unwanted Proteolysis The Bradford Method for Protein Quantitation Amino Acid Analysis by Precolumn Derivatization Identification of N-Terminal Amino Acids by High-Performance Liquid Chromatography Enzymatic Methods for Cleaving Proteins Chemical Cleavage of Proteins Chemical...
Humana Press, 1984. - 366 p. ISBN: 978-0-89603-064-0 (Print) 978-1-59259-489-4 (Online) Series: Methods in Molecular Biology, №2 The Burton Assay for DNA DABA Fluorescence Assay for Submicrogram Amounts of DNA Preparation of RNase-Free DNase by Alkylation The Isolation of Satellite DNA by Density Gradient Centrifugation The Isolation of High Molecular Weight Eukaryotic DNA...
Humana Press, 1984. — 356 р. — ISBN: 0896030628 Series: Methods in Molecular Biology, №01 The most accurate method of determIning protem concentration is probably acid hydrolysis followed by amino acid analysis. Most other methods are sensitive to the amino acid composition of the protein and absolute concentrations cannot be obtained The procedure of Lowry et al. (1) IS no...
MMB vol.032, Humana Press, 26 апр. 1994. - 490р. ISBN: 0896032698 Basic Protein and Peptide Protocols offers an excellent collection of reproducible, step-by-step laboratory methods covering three major areas: (1) the quantitation and characterization of proteins, (2) the electrophoretic and blotting procedures used in protein isolation and characterization, and (3) the...
Humana Press, 1990. - 704 p. ISBN: 0896031500 Series: Methods in Molecular Biology , №5 Animal Cell Culture, the latest volume in Humana's highly successful Methods in Molecular Biology series, provides detailed practical techniques for the culture of a broad spectrum of basic cell cell types. Chapters offer hands-on methods for creating mammalian fibroblastic cell cultures and...
2nd Edition. — Springer, 2017. — 423 p. — (Methods in Molecular Biology 1485). — ISBN10: 1493964100. This second edition expands on the previous edition with new chapters that are suitable for newcomers, as well as more detailed chapters that cover protein stability and storage, avoiding proteolysis during chromatography, protein quantitation methods including immuno-qPCR, and...
New York: Humana Press, 2022. — 342 p. Describes a broad array of different strategies and methods such as for immunogene therapy, antitumor vaccination, oncolytic virotherapy, and suicide gene therapy Provides state-of-the art protocols and overview chapters for cancer gene therapy, along with step-by-step procedures and instructions for gene therapeutic strategies, including...
Humana Press, 2016. — 339 p. — (Methods in Molecular Biology 1541). — ISBN10: 1493967010. This volume provides readers with detailed protocols covering the main cancer cytogenetics techniques needed for clinical utilization and research purposes. The chapters in this book cover topics such as chromosome preparation for myeloid malignancies; chromosome bandings; fluorescence in...
Humana, — 2022. — 317 p. — ISBN: 1071618342 This volume discusses traditional and current techniques that are successfully used to diagnose plant viruses and study molecular plant-virus interactions. The chapters in this book cover topics such as in vivo detection of double-stranded RNA, developing rice mutant using CRISPR-Cas9-based technology, protein-protein interaction...
Humana Press, 2012. — 418 p. — (Methods in Molecular Biology 802). Recent improvements in the efficiency, quality, and cost of genome-wide sequencing have prompted biologists and biomedical researchers to move away from microarray-based technology to ultra high-throughput, massively parallel genomic sequencing (Next Generation Sequencing, NGS) technology. In Next Generation...
Humana Press, 2022. — 267 p. — (Methods in Molecular Biology 2464). — ISBN 13 9781071621639. This detailed volume collects protocols from scientists who are actively engaged in developing or using protoplast technology. The book begins with chapters that focus on basic protoplast techniques and their utilities, such as protocols on protoplast isolation, transfection, and...
Humana Press, 2019. — 476 p. — (Methods in Molecular Biology 1871). This book seeks to fill in the current technology gap with a specific collection of technologies developed for the study of protein function at a proteome scale. Chapters explore topics from protein functions to other aspects of protein analysis, especially in post-translational modification, as most proteomes use...
Humana Press, 2018. — 239 p. — (Methods in Molecular Biology 1751). — ISBN: 9781493977093 EISBN 9781493977109. This detailed volume provides comprehensive practical guidance on transcriptome data analysis for a variety of scientific purposes. Beginning with general protocols, the collection moves on to explore protocols for gene characterization analysis with RNA-seq data as...
New York: Humana Press, 2017. — 298 p. — (Methods in Molecular Biology, №1652). — ISBN 978-1-4939-7218-0. This volume contains protocols specifically designed for studying cell signaling mediated by ErbB receptors, all applicable to the study of a broad range of ErbB receptor-mediated signaling from basic research to clinic applications, from cultured cells to various animal...
Humana Press, 2022. — 248 p. — (Methods in Molecular Biology 2579). — ISBN 978-1-0716-2735-8. This volume covers a broad range of cell types including cultured cell lines, primary cells, and various unicellular organisms such as fission yeast, budding yeast, parasite Leishmania amazonensis, and parasite Trypanosoma brucei. The chapters in this book are organized into four...
Humana Press, 2002. - 389 p. - Andrew Ward and a team of experienced researchers have brought together a collection of optimized classic and vanguard techniques for the identification and analysis of imprinted genes. Protocols are included for identifying and cloning imprinted genes, for analyzing imprinted gene expression, for the study of DNA methylation and...
2nd edition. — Humana Press, 2016. — 257 p. — (Methods in Molecular Biology 1376).
In this second edition methods are described to measure the synthesis of lipids such as the phosphoinositides, ceramides and sphingomyelin, as well as techniques to molecularly characterize the various kinases and phosphatases that regulate the intracellular metabolism of these lipids. Lipid...
Humana Press, 2013. — 375 p. — (Methods in Molecular Biology 935). Over the past decades, knowledge about the cellular and molecular basis underlying the visual process has remarkably increased. In this volume expert researchers in the field provide a guide of relevant and state-of-the-art methods for studying retinal homeostasis and disease. These include methods and...
2nd ed. — Humana Press, 2019. — 413 p. — (Methods in Molecular Biology 1834). — ISBN: 9781493986682, EISBN 9781493986699. This volume provides key updates on several methods from the first edition as well as including new novel techniques to address the most recent technological developments and their applications in retinal research Chapters guide readers through gene...
Humana Press, 2014. — 481 p. Series: Methods in Molecular Biology, № 1146 In this volume, expert researchers in the field detail many of the methods which are now commonly used to study flavins and flavoproteins. These include review style methods and protocols to exemplify the variety, the power and the success of modern techniques and methods in application to flavoproteins....
Methods in Molecular Biology, vol.143, Humana Press, 2001, 410 p. World-class investigators detail their most successful methods-and the theory behind them-for delineating the shape, form, and function of proteins. The protocols range from basic to advanced and include sequence alignment, the prediction of transmembrane protein structure, and the development of suitable folding...
Humana Press, 2018. — 663 p. — (Methods in Molecular Biology 1776). — ISBN 978-1-4939-7806-9. This volume details protocols on virus-derived nanoparticles (VNPs) for a number of different applications. Chapters guide readers through the production of VNPs derived from plant, animal and bacterial viruses, prokaryotic and eukaryotic expression systems, encapsulation of...
Humana Press, 2018. — 1517 p. — (Methods in Molecular Biology 1776). — ISBN 978-1-4939-7808-3. This volume details protocols on virus-derived nanoparticles (VNPs) for a number of different applications. Chapters guide readers through the production of VNPs derived from plant, animal and bacterial viruses, prokaryotic and eukaryotic expression systems, encapsulation of...
New York: Humana Press, 2023. — 246 p. This volume provides researchers with protocols that help them investigate known or putative O2 sensing proteins and pathways. The chapters in this book discuss techniques ranging from anaerobic redox midpoint measurement to approaches to control expression of globin genes, which provide detailed methods for researchers interested in...
Humana Press, 2023. — 316 p. — (Methods in Molecular Biology 2669). — ISBN 978-1-0716-3206-2. This detailed volume explores hepatic stellate cells, which have become a spotlight of liver cell biology due to their pleiotropic functions that extend well beyond extracellular matrix production to pivotal roles in hepatic homeostasis, immunity, and metabolism. The book features...
Humana Press, 2016. — 232 p. — (Methods in Molecular Biology 1304).
This detailed volume covers molecular biology, cellular biology, biomarkers, imaging, and neuropathological methods and techniques to explore multiple sclerosis (MS), with a special emphasis on disease models. With so much effort needed to elucidate basic disease mechanisms, to clone disease relevant genes, to...
Humana Press, 2010. - 559 p. - In Liposomes: Methods and Protocols, leading experts in the related fields explore cutting-edge experimental methods involving all aspects of lipids as essential components of the cell membrane. Volume 1: Pharmaceutical Nanocarriers focuses on the state-of-the-art aspects of developing liposome-based nanoscale drug delivery techniques, delving...
Humana Press, 2010. - 543 p. - With nearly one hundred years of intensive study, lipids have proven to be a vital and ever-more-promising area of cell biological research. In Liposomes: Methods and Protocols, leading experts in the related fields explore cutting-edge experimental methods involving all aspects of lipids as essential components of the cell membrane. Volume 2:...
Humana Press, 2019. — 394 p. — (Methods in Molecular Biology 2000). This volume details protocols on formulation, surface modification, characterization, and application of a variety of pharmaceutical nanocarriers such as micelles, nanoparticles, dendrimers, carbon dots, polymersomes, and others. Chapters are targeted toward investigators working in academic and industrial...
Humana Press, 2015. — xxvi+480 p. — Серия: Methods in Molecular Biology, 1264 This expert volume covers an interdisciplinary and rapidly growing area of biomedical research comprising genetic, biochemical, pathological, and clinical studies aimed at the diagnosis and therapy of human diseases which are either caused by or associated with mitochondrial dysfunction. It dedicates...
Humana Press, 2015. — xxiv+ 447. — Серия: Methods in Molecular Biology, 1265 This expert volume covers an interdisciplinary and rapidly growing area of biomedical research comprising genetic, biochemical, pathological, and clinical studies aimed at the diagnosis and therapy of human diseases which are either caused by or associated with mitochondrial dysfunction. It dedicates...
2nd edition. — Humana Press, 2021. — 462 p. — (Methods Molecular Biology 2277). — ISBN 978-1-0716-1269-9. This second edition offers 88 chapters divided among three volumes providing the most comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine. Volume III guides readers through chapters on intercellular mitochondrial transfer, Cytoplasmic...
Humana Press, 2025. — 418 р. — ISBN 978-1-0716-4175-0. This detailed volume explores techniques for researching the diverse and specialized mechanisms for mRNA degradation, both in the cytoplasm and the nucleus. From classical methods for studying RNA degradation at the single RNA level to the latest transcriptome-wide approaches involving long-read sequencing and metabolic...
Humana Press, 2025. — 418 р. — ISBN 978-1-0716-4176-7. This detailed volume explores techniques for researching the diverse and specialized mechanisms for mRNA degradation, both in the cytoplasm and the nucleus. From classical methods for studying RNA degradation at the single RNA level to the latest transcriptome-wide approaches involving long-read sequencing and metabolic...
Humana Press, 2025. — 418 р. — ISBN 978-1-0716-4176-7. This detailed volume explores techniques for researching the diverse and specialized mechanisms for mRNA degradation, both in the cytoplasm and the nucleus. From classical methods for studying RNA degradation at the single RNA level to the latest transcriptome-wide approaches involving long-read sequencing and metabolic...
Humana Press, 2025. — 418 р. — ISBN 978-1-0716-4176-7. This detailed volume explores techniques for researching the diverse and specialized mechanisms for mRNA degradation, both in the cytoplasm and the nucleus. From classical methods for studying RNA degradation at the single RNA level to the latest transcriptome-wide approaches involving long-read sequencing and metabolic...
Humana Press, 2017. — 222 p. — (Methods in Molecular Biology 1552). This volume aims to provide a new perspective on the broader usage of Hidden Markov Models (HMMs) in biology. Hidden Markov Models: Methods and Protocols guides readers through chapters on biological systems; ranging from single biomolecule, cellular level, and to organism level and the use of HMMs in unravelling...
Humana Press, 2015. — 329 p. Series: Methods in Molecular Biology, № 1305 The chapters compiled in this detailed collection outline a number of methods used to study plant mitochondria today, starting from the isolation of mitochondria to detailed analyses of RNA, protein and enzymatic activities. Given that the ability to uncover mitochondria’s unique features is underpinned by...
Humana Press, 1993. - 386 p. ISBN: 0896032442 Series: Methods in Molecular Biology, №15 Polymerase Chain Reaction. Selection of Primers for Polymerase Chain Reaction. Direct Radioactive Labeling of Polymerase Chain Reaction Products. Use of Arithmetic Polymerase Chain Reaction for Synthesis of Single-Stranded Probes for S1 Nuclease Assays. Nonradioactive Labeling of Polymerase...
Humana Press, 2017. — 256 p. — (Methods in Molecular Biology 1492). This volume explores a broad range of different genotyping techniques. Genotyping: Methods and Protocols consists of chapters that cover numerous topics such as: an overview of multiplexed microsatellite analysis; High Resolution Melt analysis and TaqMan-based assays; in situ analysis of variants in single RNA...
Springer, 2021. — 316 p. — (Methods in Molecular Biology 2237). — ISBN: 978-1071610633; ISBN: 978-1071610640. This detailed book presents a technical overview and practical methodology of a variety of antibody array formats and technologies. As advantages and disadvantages of antibody array types are explored, the volume also delves into practical applications of antibody...
Humana, 2004. — 420 p. This new edition combines updates of key chapters from the first edition with a large number of new key methodologies that have emerged more recently for studying G protein-coupled receptors (GPCRs) and events immediately downstream of their activation. The methods are focused primarily on events at the receptor level, including ligand binding, the...
Humana Press, 2000. — 272 p. Series: Methods in Molecular Biology, № 13 Because of their unique properties and relatively low environmental impact, supercritical fluids have proven highly useful in the extraction and separation of organic compounds, in particle production, as reaction media, and for the destruction of toxic waste. In Supercritical Fluid Methods and Protocols,...
2nd Edition. — Humana Press, 2013. — 524 p. — Volume 1008 of Methods in Molecular Biology. Proteins are the cell’s workers, their messengers and overseers. In these roles, proteins specifically bind small molecules, nucleic acid and other protein partners. Cellular systems are closely regulated and biologically significant changes in populations of particular protein complexes...
Humana, 2024. — 454 p. This detailed volume explores key concepts and experimental design related to Positron Emission Tomography (PET) imaging that have revolutionized our understanding of human biology. The first part focuses on recent advances in radiotracer probe development to enable the detection of materials, from large macromolecules to complicated drug-like structures....
3rd Edition. — Humana Press, 2015. — 525 p. Series: Methods in Molecular Biology, №1257 This volume provides a variety of standard protocols used to cryopreserve or freeze-dry different types of specimens. In addition, it provides chapters focused on the fundamental principles of cryopreservation, vitrification, and freeze-drying. Several state of the art microscopic,...
4th edition. — Springer, 2021. — 730 p. — (Methods in Molecular Biology 2180). — ISBN: 978-1-0716-0782-4. This fourth edition explores fully up-to-date standardly used cryopreservation, vitrification, and freeze-drying protocols for specimens that are used for research purposes, conservation of genetic reserves, and applications in agriculture and medicine. Beginning with a...
Humana Press, 2019. — 355 p. — (Neuromethods 152). — ISBN: 978-1-4939-9943-9. This volume explores the latest techniques and improved methods used to study important neurobiological and associated neuropathological conditions. Chapters cover topics such as the application of semiconductor quantum dots as photostable fluorophores used for labeling and tracking; GCaMP6...
Humana Press, 2017. — 474 p. Series: Methods in Molecular Biology, № 1558 This volume introduces bioinformatics research methods for proteins, with special focus on protein post-translational modifications (PTMs) and networks. This book is organized into four parts and covers the basic framework and major resources for analysis of protein sequence, structure, and function;...
Springer Science+Business Media LLC, Humana Press, 2017. — 217 p. — (Methods in Molecular Biology). — ISBN: 149396819X. This volume presents a collection of protocols that describe methodologies to study thermogenic fat biology from various angles. This book is divided into 2 parts. Part 1 focuses on establishing in vitro culture systems. The chapters in this section introduce...
2nd Edition. - Humana Press, 2018. - 222 p. - Volume 1699 of Methods in Molecular Biology. This volume details basic principles of experimental and computational methods for the study of microRNAs in cancer research and, therefore, provides a firm grounding for those who wish to develop further applications. Written in the highly successful Methods in Molecular Biology series...
Humana Press; 1st Edition. edition (March 3, 2011) 386 pages ISBN-10: 1617790427 Protein microarrays have been used for a wide variety of important tasks, such as identifying protein-protein interactions, discovering disease biomarkers, identifying DNA-binding specificity by protein variants, and for characterization of the humoral immune response. In Protein Microarray for...
4th Edition. — Springer, 2021. — 259 p. — (Methods in Molecular Biology 2196). — ISBN: 978-1-0716-0867-8.8-5. This fourth edition provides all new methods on yeast that have not been included in the past three editions. Divided in four parts, chapters guide readers through general applications for genetic manipulation in yeast cells, genome-wide studies, systematically...
2nd ed. — Humana Press, 2006. — 406 p. — E-ISBN: 1-59259-958-3 Series: Methods in Molecular Biology, № 313 Basic Investigations in Saccharomyces cerevisiae Isolation of Nucleic Acids Purification of Yeast Peroxisomes Isolation of Yeast Plasma Membranes Isolation of Yeast Mitochondria Extraction of Yeast Lipids Two-Dimensional Gel Electrophoresis of Total Yeast Proteins...
Humana Press, 2015. — 283 p. — (Neuromethods 104). — ISBN: 978-1-4939-2708-1. Providing a spectrum of models that is reflective of the various species that can be utilized in experimentation on disorders across a broad range of developmental disabilities, this volume collects expert contributions involved in investigation of the causes, outcomes, treatment, and prevention....
Humana Press, 2013. — 225 p. — ISBN: 1603273360, 9781603273367. Series: Methods in Molecular Biology, №972. Microarrays for simultaneous measurement of redundancy of RNA species are used in fundamental biology as well as in medical research. Statistically,a microarray may be considered as an observation of very high dimensionality equal to the number of expression levels...
Humana Press, 2013. — 225 p. — ISBN: 1603273360, 9781603273367. Series: Methods in Molecular Biology, №972. Microarrays for simultaneous measurement of redundancy of RNA species are used in fundamental biology as well as in medical research. Statistically,a microarray may be considered as an observation of very high dimensionality equal to the number of expression levels...
Humana Press, 2019. - 237 p. - Volume 1924 of Methods in Molecular Biology. This book aims to promote studies on the entire spectrum of phototropic phenomena in higher and lower plants and fungi. Chapters detail phototropism in many plant species induced by far-red, red, blue and UV lights. They also include methods for auxin biology and analysis of cytoskeleton and phototropin....
Humana Press, 2018. — 660 p. — (Methods in Molecular Biology 1836). This book provides researchers with widely used techniques for the study of virology, focusing on molecular biology and imaging to encourage mechanistic investigation of virus-host interactions. Chapters detail a broad range of methods from diagnosis, virus propagation, proteomics, haploid screening, lentiviral...
3rd ed. — Humana Press, 2025. — 395 p. This fully-updated third edition provides systems-based methodologies and innovative technologies that can be used for solving complicated problems of complex systems. A wide range of theoretical and experimental approaches are introduced for practical applications, as the content explores the basic and novel concepts in...
2nd edition. — Humana Press, 2018. — 383 p. — (Methods in Molecular Biology 1781). — ISBN: 978-1-4939-7828-1 This fully-updated book provides systems-based methodologies and innovative technologies that can be used for solving complicated problems of complex systems. A wide range of theoretical and experimental approaches are introduced for practical applications, as the content...
Nova Science Publishers, 2006. — 355 p. There are two types of brain tumours: primary brain tumours that originate in the brain and metastatic (secondary) brain tumours that originate from cancer cells that have migrated from other parts of the body. Primary brain cancer rarely spreads beyond the central nervous system, and death results from uncontrolled tumour growth within the...
Humana Press, 2023. — 382 p. — (Methods in Molecular Biology 2653). — ISBN 978-1-0716-3130-0. This volume provides readers with a collection of the latest protocols used to study plant genome editing and trait engineering. The chapters in this book are organized into five parts: genome engineering systems; machinery design and validation; delivery tools; generation and analysis...
Humana, 2025. — 376 p. — ISBN-13 : 978-1071641590 This detailed volume covers new and classic methods for vaccinia virus (the prototype poxvirus) but also extends to methods and protocols specifically tailored for mpox virus, lumpy skin disease virus, and myxoma virus. As these viruses continue to expand their territories, research into the impact of poxviruses on human and...
New York: Humana Press, 2022. — 809 p. This volume provides readers with a collection of new and classical methods, techniques, and applications used to address enduring questions about the structure and functions of the Golgi complex. The chapters in this volume cover diverse topics ranging from model systems; live and fixed cell imaging techniques; in vitro biochemical...
Springer, 2020. — 246 p. — (Methods in Molecular Biology 2176). — ISBN: 978-1-0716-0770-1. This volume presents a comprehensive collection of detailed state-of-the-art protocols for gapmer-mediated RNA knockdown from leaders in the field. Chapters detail a historical and contemporary perspective of RNase H-inducing antisense oligonucleotides called gapmers and gapmer-mediated...
Humana Press, 2022. — 234 p. — (Methods in Molecular Biology 2462). — ISBN 978-1-0716-2155-4. This volume presents an all-inclusive overview of molecular biological methods to study ABA signaling transport, and metabolism in several plant species. The chapters in this book cover topics such as biochemical and molecular biological characterization of ABA receptors; assay systems...
Humana Press, 2014 — 368p. — (Methods in Molecular Biology 1119) — ISBN: 978-1-62703-788-4 (eBook), 978-1-62703-787-7 (Hardcover). With the significant medical role that human cytomegalovirus plays in human disease (from acute disease following primary infection, to chronic disease due to life-long viral persistence), the need to better understand human cytomegalovirus biology...
New York: Humana Press, 2015. — 233 p. — (Methods in Molecular Biology 1294). This detailed volume encompasses new technological developments that specifically address questions related to adenosine 3′,5′-monophosphate (cAMP) compartmentalization, that probe relevant protein-protein interactions, that increase the spatial and temporal resolution of cAMP signal detection, and that...
Humana Press, 2005. — 293 p. — (Methods in Molecular Biology 310). — E-ISBN: 1-59259-148-6. Introduction to Chemical Genomics Chemistry for Chemical Genomics Computer-Aided Design of Small Molecules for Chemical Genomics Design, Synthesis, and Screening of Biomimetic Ligands for Affinity Chromatography The Role and Application of In Silico Docking in Chemical Genomics Research...
Humana Press, 2013. — 221 p. — (Methods in Molecular Biology 1029). Bone marrow stem cells are the most transplanted cells worldwide. These cells are used as a replacement therapy for patients suffering from a diverse number of hematopoietic diseases and immunodeficiencies. However, the use of bone marrow cells in regenerative medicine has so far remained without much success. In...
Humana Press, 2017. - 407 p. Volume 1530 of Methods in Molecular Biology. This detailed volume presents protocols for advancing the utility of nanotechnology in cancer research toward improving our understanding of cancer biology, prevention, diagnosis, and therapy. There are continuous new discoveries in the field of nanotechnology, thus creating new imaging systems or...
Humana, 2024. — 327 p. This collection explores the use of pluripotent cells to generate early embryo-like structures in vitro, with the resultant 3D embryo-like structures in vitro phenocopying many of the developmental landmarks and 3D architectures seen normally in vivo. The protocols gathered herein cover diverse aspects of the topic and exemplify some of the key...
ITexLi, 2022. — 138 p. — ISBN 1839694467 9781839694462 1839694459 9781839694455 1839694475 9781839694479. Cell culture is cell cloning technology that simulates in vivo environment conditions such as asepsis, appropriate temperature, and pH as well as certain nutritional conditions to enable cells to survive, grow, reproduce, and maintain their structure and function. Cell...
Springer, 2017. — 306 p. — (Methods in Molecular Biology 1622). This detailed collection provides an accessible compendium of up-to-date methods focused on the study of RNAi and small regulatory miRNAs in stem cells. Beginning with a brief introductory section, the volume continues by exploring methods and protocols for RNAi screening, transfection, and the knockdown of...
Humana Press, 2013. — 274 p. Series: Methods in Molecular Biology, № 958 Cotton is the most important textile and cash crop and is widely cultivated in more than 70 countries, including the United States, China, and India. Because its long life cycle and complicated genetic background, it is hard to improve cotton using traditional breeding techniques although it has made much...
Humana Press, 2010. — 261 p. Series: Methods in Molecular Biology, № 650 Recent stem cell research has revealed that miRNA and RNAi-mediated gene regulation is one of the vital determinates controlling the state of cell differentiation, with the small RNAs serving as key elements involved in regulatory network control of pluripotent cell fate determination. In this volume...
New York: Humana Press, 2014. — 254 p. — (Methods in Molecular Biology 1071). In this book, experts in the field have assembled a series of protocols describing several methods in which fluorescent protein-based reporters can be used to gain unique insights into the regulation of cellular signal transduction. Genetically encodable fluorescent biosensors have allowed researchers to...
Springer, 2019. - 369 p. - Volume 1894 of Methods in Molecular Biolog. This volume explores the latest techniques used to investigate nanotoxicity. The protocols covered in this book range from routine nanotoxicity assays to genomic and proteomic approaches in vivo and in vitro. The chapters discuss specific topics such as preparation and utilization of 3D human liver microtissue...
New York: Springer, 2022. — 435 p. This book provides state-of-the-art information on gene essentiality screenings in a wide variety of organisms, i.e. screening for protein-coding genes and other genomic elements that are required by an organism to survive under specific conditions. With a focus on the two techniques that have revolutionized the field, the collection begins...
Springer, 2022. — 435 p. — (Methods in Molecular Biology 2377). — ISBN 978-1-0716-1719-9. This book provides state-of-the-art information on gene essentiality screenings in a wide variety of organisms, i.e. screening for protein-coding genes and other genomic elements that are required by an organism to survive under specific conditions. With a focus on the two techniques that...
Humana Press, 2022. — 560 p. — (Methods in Molecular Biology 2375). — ISBN 978-1-0716-1708-3. This volume explores the latest techniques used to study the field of tissue engineered vascular grafts (TEVGs). The chapters in this book cover a wide array of topics such as deriving vascular cells from monocytes and induced pluripotent stem cells; engineering vascular grafts using...
Humana Press, 2022. — 264 p. — (Methods in Molecular Biology 2375). — ISBN 978-1-0716-1707-6. This volume explores the latest techniques used to study the field of tissue engineered vascular grafts (TEVGs). The chapters in this book cover a wide array of topics such as deriving vascular cells from monocytes and induced pluripotent stem cells; engineering vascular grafts using...
Humana Press, 2019. - 277 p. - Volume 1931 of Methods in Molecular Biology. This book details sorghum breeding technologies, grain compounds, nutrition and digestibility, biotechnology methods, broad renewable applications and an economic study. Chapters are divided into five review chapters, five case study chapters, and nine protocol chapters providing comprehensive reviews, new...
Humana, 2021. — 376 p. This collection explores detailed experimental protocols necessary for setting up a variety of in vitro cytochrome P450 (CYP) assays that are vital in selecting drug candidates in a drug discovery pipeline. Major factors affecting drug metabolism include CYP expression levels, kinetic parameters for individual CYP enzymes, CYP inhibition and induction,...
New York: Humana Press, 2022. — 243 p. This detailed volume is devoted to the recent development of quantitative experiments and computational methods driving new transforming growth factor beta (TGF-β) and other cell signaling knowledge. Many chapters cover quantitative assays for TGF-β signaling studies, with others exploring the increasing role of both modeling and...
Humana Press, 2016. — 398 p. — ISBN 978-1-4939-3306-8. The aim of this book is to improve pyrosequencing protocols as well as instrumentation for better clinical use by describing improvements and novel applications of pyrosequencing technology. Divided into five parts, the book’s thirty chapters explore advances in pyrosequencing template preparation, pyrosequencing technology...
Humana Press, 2010. - 492 p. - (Methods in Molecular Biology, Vol. 596) - With the devastating complication of cancer cells becoming simultaneously resistant to many structurally and mechanistically unrelated drugs, the efficacy of chemotherapeutic management of cancer often becomes severely limited. In Multi-Drug Resistance in Cancer, leading researchers in the field provide...
Humana Press, 2013. - 317 p. - Volume 1002 of Methods in Molecular Biology. With the advent of proteomics came the development of technologies, primarily mass spectrometry, which allowed high-throughput identification of proteins in complex mixtures. While the mass spectrometer resides at the heart of proteomics, its ability to characterize biological samples is only as good as...
London: Humana Press, 2015. — 351 с. — ISBN: 978-1-4939-2284-0 Series: Methods in Molecular Biology, № 1268 Computational peptidology is a newly emerging subfi eld that focuses on the use of computational, theoretical, and bioinformatic approaches to treat peptide-related problems. In Computational Peptidology: Methods and Protocols, expert researchers in relevant fields detail...
Humana Press, 2013. — 363 p. Series: Methods in Molecular Biology, № 1018 Understanding the molecular and cellular mechanisms underlying the development of specific neural circuits is not just an intellectual curiosity but also central to our ability to develop therapeutic approaches to repair damaged pathways in the future. In this volume, experts in the field contribute commonly...
Humana Press, 2017. — 315 p. — (Methods in Molecular Biology 1484). This thorough volume explores predicting one-dimensional functional properties, functional sites in particular, from protein sequences, an area which is getting more and more attention. Beginning with secondary structure prediction based on sequence only, the book continues by exploring secondary structure...
Humana Press, 2005. — 499 p. — e-ISBN: 1-59259-907-9 Series: Methods in Molecular Biology, № 304 Retroviruses are a large and diverse family of enveloped, single-strand RNA viruses characterized by the unique replicative strategy that includes reverse transcription of the virion RNA into linear double-stranded DNA and the subsequent integration of this DNA into the genome of...
Humana Press, 2019. — 318 p. — (Methods in Molecular Biology 1888). This volume explores the latest available wet-lab techniques and computational methods to study in-cell small-molecule behavior and interactions with their targets. The chapters in this book discuss topics such as disease-relevant models for chemical biology studies, target engagement using cellular thermal shift...
New York: Humana Press, 2023. — 410 p. This detailed new edition broadens the scope of the first edition by moving beyond classical display technologies. This book explores methodologies for the generation of natively paired antibody libraries, single cell technologies, alternative scaffolds, and in silico antibody sequence assessments are described. The application of these...
Humana Press, 2011. — 430 p. — (Methods in Molecular Biology 747). — ISBN: 978-1-61779-135-2. As a key component of human survival, a safe and sufficient food supply is essential for a healthy and productive population throughout the world, so assurance that the food supply is clean and free of harmful substances is a global concern. In Mass Spectrometry in Food Safety: Methods...
Humana, 2025. — 735 p. — ISBN-13 : 978-1071644058 This volume covers a broad range of procedures and protocols that discuss the steps for designing experimental setups for studying sperm, including analytical tools for diverse species. The chapters in this book are organized into 5 parts: Part One looks at the latest classical methods of analysis of sperm quality parameters;...
Springer Science+Business Media LLC, 2017. — 314 p. — (Methods in Molecular Biology 1594) — ISBN: 1493969323. This volume provides laboratory protocols essential for studies on lysosomal biology. Chapters aim to guide researchers in their exploration of lysosomes, both under normal conditions and in pathological processes. Written in the highly successful Methods in Molecular...
Humana Press, 2024. — 201 p. — (Methods in Molecular Biology 2773). — ISBN 978-1-0716-3713-5. This detailed volume presents a variety of mouse models for cancer studies, from leukemia and lymphoma models to different types of subcutaneous and orthotopic models. Models for the healing process and the assessment of the immune response after local ablative therapies are also...
Humana Press, 2024. — 201 p. — (Methods in Molecular Biology 2773). — ISBN 978-1-0716-3714-2. This detailed volume presents a variety of mouse models for cancer studies, from leukemia and lymphoma models to different types of subcutaneous and orthotopic models. Models for the healing process and the assessment of the immune response after local ablative therapies are also...
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